Mutagenetix > Incidental Mutation

Incidental Mutation 'R9559:Prl3d1'

720928

Institutional Source

Beutler Lab

Gene Symbol

Prl3d1

Ensembl Gene

ENSMUSG00000057170

Gene Name

prolactin family 3, subfamily d, member 1

Synonyms

Pl-1, Csh1, PL-Ia, prolactin-like 2, Pl1, placental lactogen 1, mPL-I

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R9559 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

27278173-27284241 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 27280470 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 73 (T73I)

Ref Sequence

ENSEMBL: ENSMUSP00000080731 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082079] [ENSMUST00000225330]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000082079
AA Change: T73I

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000080731
Gene: ENSMUSG00000057170
AA Change: T73I

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	17	224	5.1e-73	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000225330
AA Change: T74I

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	G	A	7: 120,021,019 (GRCm39)		probably null	Het
Ahnak2	C	T	12: 112,749,782 (GRCm39)		probably null	Het
Arhgef37	A	T	18: 61,640,267 (GRCm39)		probably null	Het
Asb14	T	C	14: 26,637,052 (GRCm39)	V598A	possibly damaging	Het
Baz1b	T	A	5: 135,216,532 (GRCm39)	F10Y	probably benign	Het
Bbc3	T	C	7: 16,047,660 (GRCm39)	V128A	probably benign	Het
C130073F10Rik	T	A	4: 101,747,946 (GRCm39)	Y76F	possibly damaging	Het
Cdc42bpa	G	A	1: 179,939,459 (GRCm39)		probably null	Het
Cfh	G	T	1: 140,030,275 (GRCm39)	P884Q	probably benign	Het
Cltc	T	C	11: 86,613,086 (GRCm39)	Y479C	probably damaging	Het
Col18a1	C	T	10: 76,913,630 (GRCm39)	G477E	probably damaging	Het
Col7a1	A	G	9: 108,786,360 (GRCm39)	N530S	unknown	Het
Csmd2	G	T	4: 128,438,561 (GRCm39)	G3047C		Het
Cyb5r3	C	A	15: 83,043,123 (GRCm39)	E190*	probably null	Het
Dnah3	A	T	7: 119,650,951 (GRCm39)	V983D	probably benign	Het
Fcer1a	T	G	1: 173,052,884 (GRCm39)	Y104S	possibly damaging	Het
Fer1l6	A	G	15: 58,429,759 (GRCm39)	T169A	possibly damaging	Het
Gimap9	A	T	6: 48,655,134 (GRCm39)	K240N	probably benign	Het
Gmeb1	A	C	4: 131,953,140 (GRCm39)	V542G	probably benign	Het
Grb10	C	A	11: 11,895,535 (GRCm39)	R318L	probably damaging	Het
Gucy1b1	T	A	3: 81,947,054 (GRCm39)	D385V	possibly damaging	Het
Hdgfl1	C	T	13: 26,953,239 (GRCm39)	G278E	probably damaging	Het
Il18r1	T	A	1: 40,528,793 (GRCm39)	I279K	probably benign	Het
Il21r	A	G	7: 125,232,027 (GRCm39)	D485G	probably damaging	Het
Jarid2	C	T	13: 45,068,253 (GRCm39)	R1092W	possibly damaging	Het
Kdm4b	T	C	17: 56,693,228 (GRCm39)	L355P	probably damaging	Het
Kif26a	C	T	12: 112,142,004 (GRCm39)	R753W	probably damaging	Het
Krtcap2	C	T	3: 89,154,178 (GRCm39)	T33I	probably damaging	Het
Mettl17	G	A	14: 52,129,009 (GRCm39)		probably null	Het
Mov10	A	G	3: 104,708,277 (GRCm39)	F491L		Het
Mterf1a	C	T	5: 3,941,807 (GRCm39)	W20*	probably null	Het
Nck2	T	C	1: 43,593,207 (GRCm39)	V138A	probably damaging	Het
Or2h2c	C	T	17: 37,422,509 (GRCm39)	V122M	possibly damaging	Het
Or5ac24	C	A	16: 59,165,368 (GRCm39)	G232V	probably damaging	Het
Or5ak24	A	C	2: 85,260,753 (GRCm39)	V140G	possibly damaging	Het
Or7e173	A	G	9: 19,939,216 (GRCm39)	V6A	probably benign	Het
Osmr	A	G	15: 6,882,027 (GRCm39)	V39A	probably damaging	Het
Plekhh2	A	G	17: 84,899,017 (GRCm39)	H998R	probably damaging	Het
Prb1a	G	A	6: 132,184,388 (GRCm39)	S415F	unknown	Het
Scgn	A	G	13: 24,137,921 (GRCm39)	L250P	probably damaging	Het
Scyl3	T	C	1: 163,779,773 (GRCm39)	I580T	probably benign	Het
Sfi1	ACA	ACATCTTCCCAAAGCCAGTCA	11: 3,103,382 (GRCm39)		probably benign	Het
Shank2	A	T	7: 143,585,041 (GRCm39)	Q14L	probably benign	Het
Slc22a12	T	A	19: 6,587,686 (GRCm39)	N423Y	probably damaging	Het
Tenm4	T	A	7: 96,473,056 (GRCm39)	S951T	probably benign	Het
Ubash3b	G	A	9: 40,954,926 (GRCm39)	P195S	probably damaging	Het
Vmn1r61	A	G	7: 5,613,498 (GRCm39)	F272S	probably damaging	Het
Zbtb41	A	T	1: 139,358,053 (GRCm39)	I454F	probably benign	Het
Zfp112	G	T	7: 23,826,108 (GRCm39)	C696F	probably damaging	Het

Other mutations in Prl3d1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1104:Prl3d1	UTSW	13	27,283,992 (GRCm39)	missense	probably benign	0.25
R1389:Prl3d1	UTSW	13	27,282,693 (GRCm39)	nonsense	probably null
R2253:Prl3d1	UTSW	13	27,278,981 (GRCm39)	missense	possibly damaging	0.95
R5589:Prl3d1	UTSW	13	27,278,927 (GRCm39)	missense	probably damaging	1.00
R5739:Prl3d1	UTSW	13	27,283,995 (GRCm39)	missense	probably benign	0.29
R7184:Prl3d1	UTSW	13	27,282,619 (GRCm39)	missense	probably damaging	1.00
R7203:Prl3d1	UTSW	13	27,282,684 (GRCm39)	missense	possibly damaging	0.49
R7637:Prl3d1	UTSW	13	27,284,052 (GRCm39)	missense	probably damaging	1.00
R7655:Prl3d1	UTSW	13	27,284,018 (GRCm39)	missense	possibly damaging	0.80
R7656:Prl3d1	UTSW	13	27,284,018 (GRCm39)	missense	possibly damaging	0.80
R8050:Prl3d1	UTSW	13	27,284,011 (GRCm39)	nonsense	probably null
R8964:Prl3d1	UTSW	13	27,283,926 (GRCm39)	missense	possibly damaging	0.86
R9546:Prl3d1	UTSW	13	27,278,982 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- CTAGCTTGCACATGTAGGAGAAG -3'
(R):5'- ATTCTGAGATTACCACACCTCTATG -3'

Sequencing Primer
(F):5'- CTTGCACATGTAGGAGAAGTAAAG -3'
(R):5'- CCACACCTCTATGGGCTTAATAATC -3'

Posted On

2022-08-09