Mutagenetix > Incidental Mutation

Incidental Mutation 'R9561:Zfp81'

721033

Institutional Source

Beutler Lab

Gene Symbol

Zfp81

Ensembl Gene

ENSMUSG00000003929

Gene Name

zinc finger protein 81

Synonyms

Hszfp36, D330034E10Rik, KRAB13, C330034P10Rik, Zfp78

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.339) question?

Stock #

R9561 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

33548316-33577852 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 33553774 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 347 (T347A)

Ref Sequence

ENSEMBL: ENSMUSP00000050728 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054072]

AlphaFold

B8JJZ7

Predicted Effect

probably benign
Transcript: ENSMUST00000054072
AA Change: T347A

PolyPhen 2 Score 0.154 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000050728
Gene: ENSMUSG00000003929
AA Change: T347A

Domain	Start	End	E-Value	Type
KRAB	4	55	1.15e-14	SMART
ZnF_C2H2	222	244	1.04e-3	SMART
ZnF_C2H2	250	272	1.53e-1	SMART
ZnF_C2H2	278	300	1.33e-1	SMART
ZnF_C2H2	306	328	5.21e-4	SMART
ZnF_C2H2	334	356	2.2e-2	SMART
ZnF_C2H2	362	384	1.58e-3	SMART
ZnF_C2H2	390	412	1.64e-1	SMART
ZnF_C2H2	418	440	7.67e-2	SMART
ZnF_C2H2	446	468	1.89e-1	SMART
ZnF_C2H2	474	496	2.86e-1	SMART
ZnF_C2H2	502	524	1.08e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930562C15Rik	A	G	16: 4,680,980 (GRCm39)	I106V	possibly damaging	Het
Aars1	C	A	8: 111,763,615 (GRCm39)	F18L	probably damaging	Het
Abca7	C	T	10: 79,837,535 (GRCm39)	T473I	probably damaging	Het
Ap1m2	A	T	9: 21,209,524 (GRCm39)	I384N	probably damaging	Het
Atg16l2	G	A	7: 100,948,248 (GRCm39)	Q99*	probably null	Het
Bptf	A	T	11: 106,964,954 (GRCm39)	Y1413*	probably null	Het
Cat	T	C	2: 103,307,250 (GRCm39)	T28A	probably damaging	Het
Ccdc65	T	A	15: 98,620,759 (GRCm39)	V418D	probably benign	Het
Cd226	A	C	18: 89,265,444 (GRCm39)	I241L	probably benign	Het
Crybg1	A	G	10: 43,873,428 (GRCm39)	S1227P	probably benign	Het
Csn2	C	T	5: 87,842,794 (GRCm39)	A78T	probably benign	Het
Cyp21a1	T	A	17: 35,021,652 (GRCm39)	H305L	possibly damaging	Het
Ddx55	A	G	5: 124,706,707 (GRCm39)	E572G	possibly damaging	Het
Dguok	A	G	6: 83,467,548 (GRCm39)	Y100H	probably damaging	Het
Dync1h1	T	A	12: 110,615,533 (GRCm39)	S2955R	probably damaging	Het
Etfbkmt	A	T	6: 149,045,640 (GRCm39)		probably benign	Het
Fam222a	T	A	5: 114,749,347 (GRCm39)	I181N	probably damaging	Het
Fam24b	T	C	7: 130,927,877 (GRCm39)	D104G	probably benign	Het
Fbn2	A	T	18: 58,181,611 (GRCm39)	C1883*	probably null	Het
Fchsd2	T	G	7: 100,920,778 (GRCm39)	L461R	probably benign	Het
Hdgfl1	C	T	13: 26,953,239 (GRCm39)	G278E	probably damaging	Het
Hectd4	G	A	5: 121,472,532 (GRCm39)	R2756Q	possibly damaging	Het
Ints14	A	G	9: 64,882,932 (GRCm39)	D261G	probably damaging	Het
Mapkapk5	C	T	5: 121,672,490 (GRCm39)	A163T	probably benign	Het
Micu3	A	T	8: 40,835,156 (GRCm39)	K504*	probably null	Het
Myh1	C	G	11: 67,108,618 (GRCm39)	H1345D	possibly damaging	Het
Myh7	C	A	14: 55,216,146 (GRCm39)	R1289L	probably damaging	Het
Neb	G	T	2: 52,132,068 (GRCm39)	H280Q		Het
Nrbp1	T	A	5: 31,404,771 (GRCm39)		probably null	Het
Oacyl	T	A	18: 65,831,414 (GRCm39)	V17D	possibly damaging	Het
Obsl1	C	A	1: 75,480,157 (GRCm39)	R463L	possibly damaging	Het
Or1f19	T	A	16: 3,410,725 (GRCm39)	L155Q	probably damaging	Het
Or8b40	G	A	9: 38,028,010 (GRCm39)	S311N	probably benign	Het
Pcnt	A	T	10: 76,217,128 (GRCm39)	C2184*	probably null	Het
Pfpl	G	C	19: 12,406,297 (GRCm39)	E183Q	probably damaging	Het
Phrf1	T	G	7: 140,834,815 (GRCm39)	S198A	unknown	Het
Plekhg2	C	G	7: 28,064,249 (GRCm39)	A431P	probably damaging	Het
Ppp1r13b	C	T	12: 111,810,077 (GRCm39)	D244N	probably damaging	Het
Raph1	T	C	1: 60,564,887 (GRCm39)	E200G	possibly damaging	Het
Rnf6	A	G	5: 146,147,936 (GRCm39)	S361P	probably benign	Het
Scgb2b19	G	A	7: 32,978,039 (GRCm39)	A86V	probably benign	Het
Sec23b	T	A	2: 144,408,728 (GRCm39)	C138S	possibly damaging	Het
Slco1c1	T	C	6: 141,505,606 (GRCm39)	S511P	possibly damaging	Het
Tas2r130	A	G	6: 131,607,175 (GRCm39)	S207P	probably damaging	Het
Tdpoz1	A	T	3: 93,578,540 (GRCm39)	H81Q	probably benign	Het
Usp8	A	G	2: 126,578,414 (GRCm39)	K289E	probably damaging	Het
Vmn1r18	A	C	6: 57,367,202 (GRCm39)	N117K	probably benign	Het
Vps13c	G	T	9: 67,872,794 (GRCm39)	G3270V	probably damaging	Het
Wdr20	A	G	12: 110,760,187 (GRCm39)	T358A	probably benign	Het
Wwc1	A	C	11: 35,870,796 (GRCm39)		probably null	Het
Yars2	G	T	16: 16,127,242 (GRCm39)	V436L	possibly damaging	Het
Zfp609	G	A	9: 65,604,512 (GRCm39)	Q1324*	probably null	Het
Zgpat	T	C	2: 181,021,366 (GRCm39)	V292A	probably benign	Het

Other mutations in Zfp81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
feuer	UTSW	17	33,553,307 (GRCm39)	nonsense	probably null
R0143:Zfp81	UTSW	17	33,554,095 (GRCm39)	missense	possibly damaging	0.85
R0220:Zfp81	UTSW	17	33,555,698 (GRCm39)	missense	possibly damaging	0.93
R0520:Zfp81	UTSW	17	33,553,351 (GRCm39)	missense	probably damaging	0.97
R0611:Zfp81	UTSW	17	33,553,593 (GRCm39)	missense	probably benign	0.02
R1171:Zfp81	UTSW	17	33,554,254 (GRCm39)	missense	probably benign
R1613:Zfp81	UTSW	17	33,553,757 (GRCm39)	missense	probably damaging	0.98
R1779:Zfp81	UTSW	17	33,554,080 (GRCm39)	missense	probably benign	0.06
R1970:Zfp81	UTSW	17	33,554,475 (GRCm39)	missense	probably benign
R2063:Zfp81	UTSW	17	33,554,278 (GRCm39)	missense	probably benign	0.03
R2314:Zfp81	UTSW	17	33,553,597 (GRCm39)	missense	probably damaging	0.99
R2898:Zfp81	UTSW	17	33,553,274 (GRCm39)	nonsense	probably null
R3115:Zfp81	UTSW	17	33,553,537 (GRCm39)	missense	possibly damaging	0.80
R4207:Zfp81	UTSW	17	33,553,890 (GRCm39)	missense	probably damaging	1.00
R4497:Zfp81	UTSW	17	33,553,677 (GRCm39)	missense	possibly damaging	0.47
R4498:Zfp81	UTSW	17	33,553,677 (GRCm39)	missense	possibly damaging	0.47
R5756:Zfp81	UTSW	17	33,553,307 (GRCm39)	nonsense	probably null
R5964:Zfp81	UTSW	17	33,555,819 (GRCm39)	missense	probably damaging	0.99
R6671:Zfp81	UTSW	17	33,554,413 (GRCm39)	missense	probably benign	0.17
R7728:Zfp81	UTSW	17	33,555,791 (GRCm39)	missense	possibly damaging	0.95
R9047:Zfp81	UTSW	17	33,553,387 (GRCm39)	missense	probably damaging	1.00
R9800:Zfp81	UTSW	17	33,554,411 (GRCm39)	nonsense	probably null
Z1176:Zfp81	UTSW	17	33,553,803 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TACATTGGTATAAGTTCTACCCACT -3'
(R):5'- ATGTAGTTATGGTCAGTCATATGAAAG -3'

Sequencing Primer
(F):5'- GTGAATGCATGCCCACATTG -3'
(R):5'- GGAAAGCTTTCATTCATCCAAGGGC -3'

Posted On

2022-08-09