Mutagenetix > Incidental Mutation

Incidental Mutation 'R9563:Vmn2r65'

721212

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r65

Ensembl Gene

ENSMUSG00000066372

Gene Name

vomeronasal 2, receptor 65

Synonyms

ENSMUSG00000070600

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R9563 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

84589377-84613217 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 84589880 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 679 (Q679*)

Ref Sequence

ENSEMBL: ENSMUSP00000036551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044583]

AlphaFold

G3X931

Predicted Effect

probably null
Transcript: ENSMUST00000044583
AA Change: Q679*

SMART Domains

Protein: ENSMUSP00000036551
Gene: ENSMUSG00000066372
AA Change: Q679*

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	73	435	5.6e-41	PFAM
Pfam:NCD3G	501	553	1.3e-21	PFAM
Pfam:7tm_3	584	821	2.3e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 127 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	T	A	6: 121,645,009 (GRCm39)	D953E	probably damaging	Het
Acod1	A	C	14: 103,287,109 (GRCm39)	Y80S	probably damaging	Het
Actr5	A	G	2: 158,470,135 (GRCm39)	D255G	probably damaging	Het
Akna	A	G	4: 63,312,944 (GRCm39)	V393A	probably damaging	Het
Amotl1	T	C	9: 14,473,513 (GRCm39)	K562R	possibly damaging	Het
Amz1	T	A	5: 140,738,133 (GRCm39)	D464E	probably damaging	Het
Anapc1	G	A	2: 128,505,980 (GRCm39)	Q619*	probably null	Het
Arfgef3	C	T	10: 18,522,275 (GRCm39)	G584R	probably damaging	Het
Armt1	AC	A	10: 4,400,848 (GRCm39)		probably null	Het
Atr	G	C	9: 95,802,833 (GRCm39)	V1832L	probably damaging	Het
Bsn	T	C	9: 107,984,616 (GRCm39)	D374G		Het
Cap1	A	G	4: 122,758,505 (GRCm39)	V225A	probably benign	Het
Cc2d1a	A	G	8: 84,863,758 (GRCm39)	S562P	probably benign	Het
Ccdc30	T	C	4: 119,250,821 (GRCm39)	N141S	possibly damaging	Het
Cdc42bpb	A	G	12: 111,265,762 (GRCm39)	V1390A	possibly damaging	Het
Cdc42ep1	A	T	15: 78,733,782 (GRCm39)	H294L	probably benign	Het
Col6a6	C	A	9: 105,572,952 (GRCm39)	G2185C	probably benign	Het
Col7a1	T	C	9: 108,791,809 (GRCm39)	V1232A	unknown	Het
Copb1	A	G	7: 113,836,034 (GRCm39)	I449T	possibly damaging	Het
Cplane1	T	G	15: 8,216,563 (GRCm39)	L594V	probably benign	Het
Ctdsp2	A	G	10: 126,832,040 (GRCm39)	D216G	probably damaging	Het
Cttnbp2	T	A	6: 18,367,382 (GRCm39)	K1645N	probably benign	Het
Cttnbp2	A	T	6: 18,427,467 (GRCm39)	L738*	probably null	Het
Cul9	T	A	17: 46,820,897 (GRCm39)	T1927S	probably benign	Het
Cyfip2	A	G	11: 46,151,707 (GRCm39)	C531R	probably benign	Het
Cyp2j6	A	G	4: 96,414,245 (GRCm39)	I340T	probably damaging	Het
Daam1	A	G	12: 71,992,251 (GRCm39)	E322G	unknown	Het
Dab2ip	T	A	2: 35,609,915 (GRCm39)	L710*	probably null	Het
Degs1l	G	T	1: 180,882,810 (GRCm39)	V191F	possibly damaging	Het
Dph1	T	C	11: 75,076,825 (GRCm39)	T60A	possibly damaging	Het
Eme1	T	C	11: 94,541,339 (GRCm39)	D161G	probably benign	Het
Epha3	C	T	16: 63,366,510 (GRCm39)	G980D	possibly damaging	Het
Eri3	T	A	4: 117,422,013 (GRCm39)	V136E	probably benign	Het
Espl1	T	A	15: 102,228,233 (GRCm39)	I1669N	possibly damaging	Het
Exoc6	T	C	19: 37,588,071 (GRCm39)	I568T	probably damaging	Het
F3	T	A	3: 121,527,822 (GRCm39)	L85H		Het
Fam186b	G	A	15: 99,177,616 (GRCm39)	A570V	probably damaging	Het
Fbxw14	T	C	9: 109,106,335 (GRCm39)	Y234C	probably benign	Het
Fbxw25	T	A	9: 109,483,676 (GRCm39)	N179Y		Het
Foxr1	T	A	9: 44,352,199 (GRCm39)		probably benign	Het
Gas8	T	A	8: 124,263,179 (GRCm39)	V452E	possibly damaging	Het
Gdpd4	T	C	7: 97,649,369 (GRCm39)	V450A	probably damaging	Het
Greb1	T	C	12: 16,774,824 (GRCm39)	Y191C	probably benign	Het
H3c11	T	C	13: 21,967,221 (GRCm39)	D107G	probably damaging	Het
Haus3	G	A	5: 34,325,300 (GRCm39)	R120W	probably benign	Het
Herc1	G	T	9: 66,294,193 (GRCm39)		probably null	Het
Hyal2	T	C	9: 107,447,844 (GRCm39)	W166R	probably damaging	Het
Inka1	T	A	9: 107,861,488 (GRCm39)	Y276F	probably benign	Het
Itga7	C	T	10: 128,789,669 (GRCm39)	A1003V	probably damaging	Het
Itga8	T	A	2: 12,165,219 (GRCm39)	I797F	possibly damaging	Het
Kank2	T	C	9: 21,705,852 (GRCm39)	T389A	possibly damaging	Het
Kcnb2	T	A	1: 15,779,737 (GRCm39)	I203N	probably damaging	Het
Kif21b	T	A	1: 136,077,166 (GRCm39)	L396Q	probably damaging	Het
Kng1	T	G	16: 22,879,170 (GRCm39)	I78S	probably damaging	Het
Ldlrad4	T	C	18: 68,387,551 (GRCm39)	S288P	probably benign	Het
Lmntd2	A	G	7: 140,790,701 (GRCm39)	S516P		Het
Lrp1b	T	A	2: 41,185,711 (GRCm39)	I1449F		Het
Lrrk2	T	A	15: 91,634,043 (GRCm39)	I1380K	possibly damaging	Het
Lvrn	T	G	18: 47,017,506 (GRCm39)	I612S	probably damaging	Het
Lyst	T	C	13: 13,812,408 (GRCm39)	L940P	probably benign	Het
Man2a2	T	A	7: 80,006,101 (GRCm39)	M996L	probably benign	Het
Map3k3	T	A	11: 106,041,860 (GRCm39)	I413N	probably damaging	Het
Map4k5	A	G	12: 69,863,167 (GRCm39)	V629A	probably benign	Het
Mapre2	A	G	18: 24,023,981 (GRCm39)	E325G	unknown	Het
Mbnl1	A	G	3: 60,520,715 (GRCm39)	N101S	probably benign	Het
Meltf	T	A	16: 31,703,869 (GRCm39)	C199S	probably damaging	Het
Mmab	T	C	5: 114,574,850 (GRCm39)	E141G	probably benign	Het
Mtcl2	G	A	2: 156,902,182 (GRCm39)	R278C	probably damaging	Het
Mtus2	T	C	5: 148,250,217 (GRCm39)	L1317P		Het
Muc6	T	A	7: 141,217,783 (GRCm39)	T2297S	possibly damaging	Het
Myom2	A	T	8: 15,158,399 (GRCm39)	K784*	probably null	Het
Nek1	A	G	8: 61,577,157 (GRCm39)	E1118G	probably benign	Het
Nrtn	A	G	17: 57,058,416 (GRCm39)	V195A	probably damaging	Het
Nsd3	G	A	8: 26,204,230 (GRCm39)	V420I		Het
Or52e4	T	C	7: 104,706,281 (GRCm39)	L276P	possibly damaging	Het
Or52z13	A	T	7: 103,247,225 (GRCm39)	Q234L	probably benign	Het
Or5ac23	T	C	16: 59,149,765 (GRCm39)	I36V	probably benign	Het
Or5b99	T	A	19: 12,976,983 (GRCm39)	I211N	probably damaging	Het
Or5t9	A	G	2: 86,659,098 (GRCm39)	M1V	probably null	Het
Or6ae1	G	A	7: 139,742,233 (GRCm39)	T210I	probably damaging	Het
Or6c33	T	C	10: 129,853,287 (GRCm39)	I19T	probably benign	Het
Or8b56	A	T	9: 38,739,014 (GRCm39)	H3L	probably benign	Het
Pde10a	G	T	17: 9,020,710 (GRCm39)	A216S	unknown	Het
Phldb2	T	C	16: 45,645,247 (GRCm39)	T400A	possibly damaging	Het
Pkd1l1	T	A	11: 8,815,502 (GRCm39)	T1920S		Het
Ptgs2	T	A	1: 149,981,419 (GRCm39)	N567K	probably benign	Het
Pwp1	T	A	10: 85,712,370 (GRCm39)	D141E	probably damaging	Het
Rab3ip	T	C	10: 116,754,668 (GRCm39)	E296G	probably null	Het
Rb1cc1	A	G	1: 6,314,339 (GRCm39)	K337R	probably benign	Het
Rbp3	A	G	14: 33,677,477 (GRCm39)	D475G	probably damaging	Het
Ric3	T	C	7: 108,637,997 (GRCm39)	E253G	possibly damaging	Het
Rictor	A	T	15: 6,797,562 (GRCm39)	N306I	possibly damaging	Het
Rnf214	A	T	9: 45,811,141 (GRCm39)	V174E	possibly damaging	Het
Robo3	A	G	9: 37,340,900 (GRCm39)	F124S	probably damaging	Het
Rrad	A	G	8: 105,355,324 (GRCm39)	F228S	probably damaging	Het
Scn3a	A	T	2: 65,291,595 (GRCm39)	M1717K	probably damaging	Het
Scn3b	A	G	9: 40,193,729 (GRCm39)	D152G	probably benign	Het
Scn5a	C	A	9: 119,315,803 (GRCm39)	R1635L	probably damaging	Het
Sec61b	A	G	4: 47,483,049 (GRCm39)	Y93C	probably damaging	Het
Shprh	T	A	10: 11,042,235 (GRCm39)	Y739*	probably null	Het
Skor2	C	T	18: 76,946,376 (GRCm39)	H33Y	unknown	Het
Slc26a7	T	C	4: 14,519,496 (GRCm39)	N508S	probably benign	Het
Slc7a12	A	G	3: 14,564,360 (GRCm39)	I349V	possibly damaging	Het
Slit1	T	C	19: 41,596,874 (GRCm39)	Y1075C	probably damaging	Het
Sltm	C	T	9: 70,480,841 (GRCm39)	A231V	unknown	Het
Smg1	A	G	7: 117,812,208 (GRCm39)	S52P	unknown	Het
Sorl1	A	G	9: 41,957,893 (GRCm39)	Y584H	probably damaging	Het
Spmip2	A	T	3: 79,356,616 (GRCm39)	M140L	probably benign	Het
Ssbp1	T	A	6: 40,454,968 (GRCm39)	S142R	probably benign	Het
Stard3	A	G	11: 98,270,797 (GRCm39)		probably null	Het
Strn3	G	T	12: 51,674,300 (GRCm39)	H531N	possibly damaging	Het
Stx17	A	G	4: 48,180,739 (GRCm39)	T195A	probably damaging	Het
Tekt2	C	T	4: 126,217,444 (GRCm39)	R207H	probably damaging	Het
Tmed5	A	C	5: 108,280,100 (GRCm39)		probably null	Het
Tmem132a	C	T	19: 10,838,960 (GRCm39)	R494H	probably benign	Het
Tom1	A	G	8: 75,787,177 (GRCm39)	K406E	probably benign	Het
Trav5d-4	A	T	14: 53,239,473 (GRCm39)	N43Y	probably damaging	Het
Trbv19	G	T	6: 41,155,945 (GRCm39)	K105N	possibly damaging	Het
Trim34a	C	T	7: 103,910,328 (GRCm39)	Q377*	probably null	Het
Trp53bp2	T	A	1: 182,276,378 (GRCm39)	N786K	probably benign	Het
Trpc3	A	G	3: 36,705,683 (GRCm39)	M504T	probably benign	Het
Ttc28	A	T	5: 111,371,092 (GRCm39)	M545L	probably benign	Het
Uggt1	A	C	1: 36,204,627 (GRCm39)	V996G	possibly damaging	Het
Wdfy4	T	C	14: 32,692,833 (GRCm39)	I2958V		Het
Zbtb37	C	T	1: 160,847,840 (GRCm39)	S389N	possibly damaging	Het
Zfp235	T	C	7: 23,841,669 (GRCm39)	V696A	possibly damaging	Het
Zzef1	T	A	11: 72,765,732 (GRCm39)	C1420S	probably damaging	Het

Other mutations in Vmn2r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Vmn2r65	APN	7	84,592,761 (GRCm39)	missense	probably damaging	0.99
IGL01104:Vmn2r65	APN	7	84,589,996 (GRCm39)	missense	possibly damaging	0.93
IGL01154:Vmn2r65	APN	7	84,592,729 (GRCm39)	missense	probably benign	0.00
IGL01453:Vmn2r65	APN	7	84,589,708 (GRCm39)	missense	probably damaging	1.00
IGL01675:Vmn2r65	APN	7	84,596,587 (GRCm39)	missense	probably benign	0.00
IGL01748:Vmn2r65	APN	7	84,589,507 (GRCm39)	missense	probably damaging	1.00
IGL01997:Vmn2r65	APN	7	84,589,978 (GRCm39)	missense	probably damaging	1.00
IGL02527:Vmn2r65	APN	7	84,595,724 (GRCm39)	missense	possibly damaging	0.82
IGL02617:Vmn2r65	APN	7	84,589,549 (GRCm39)	missense	probably damaging	1.00
IGL02676:Vmn2r65	APN	7	84,589,381 (GRCm39)	missense	possibly damaging	0.86
IGL03084:Vmn2r65	APN	7	84,592,354 (GRCm39)	missense	probably damaging	1.00
A4554:Vmn2r65	UTSW	7	84,595,791 (GRCm39)	missense	probably damaging	0.96
PIT4651001:Vmn2r65	UTSW	7	84,595,461 (GRCm39)	missense	probably benign	0.12
R0322:Vmn2r65	UTSW	7	84,595,756 (GRCm39)	missense	probably benign	0.01
R0453:Vmn2r65	UTSW	7	84,595,442 (GRCm39)	missense	probably benign	0.03
R0862:Vmn2r65	UTSW	7	84,592,853 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1508:Vmn2r65	UTSW	7	84,589,886 (GRCm39)	missense	probably benign	0.00
R1687:Vmn2r65	UTSW	7	84,590,026 (GRCm39)	missense	probably benign	0.02
R1876:Vmn2r65	UTSW	7	84,595,505 (GRCm39)	missense	probably damaging	1.00
R2136:Vmn2r65	UTSW	7	84,592,781 (GRCm39)	missense	probably damaging	0.96
R2259:Vmn2r65	UTSW	7	84,590,119 (GRCm39)	missense	possibly damaging	0.86
R2338:Vmn2r65	UTSW	7	84,590,051 (GRCm39)	missense	possibly damaging	0.86
R2880:Vmn2r65	UTSW	7	84,613,094 (GRCm39)	missense	probably damaging	1.00
R3410:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3411:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3770:Vmn2r65	UTSW	7	84,589,623 (GRCm39)	missense	probably damaging	1.00
R3800:Vmn2r65	UTSW	7	84,589,738 (GRCm39)	missense	possibly damaging	0.93
R3850:Vmn2r65	UTSW	7	84,595,859 (GRCm39)	missense	probably benign	0.00
R4105:Vmn2r65	UTSW	7	84,595,691 (GRCm39)	missense	probably benign	0.03
R4568:Vmn2r65	UTSW	7	84,596,677 (GRCm39)	missense	probably damaging	1.00
R4677:Vmn2r65	UTSW	7	84,613,082 (GRCm39)	missense	possibly damaging	0.55
R4768:Vmn2r65	UTSW	7	84,596,602 (GRCm39)	missense	probably damaging	1.00
R4778:Vmn2r65	UTSW	7	84,592,801 (GRCm39)	missense	possibly damaging	0.47
R5057:Vmn2r65	UTSW	7	84,589,819 (GRCm39)	missense	probably damaging	1.00
R5279:Vmn2r65	UTSW	7	84,589,849 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r65	UTSW	7	84,595,542 (GRCm39)	missense	probably damaging	0.99
R5394:Vmn2r65	UTSW	7	84,595,862 (GRCm39)	missense	probably benign	0.04
R5487:Vmn2r65	UTSW	7	84,595,529 (GRCm39)	missense	possibly damaging	0.76
R5580:Vmn2r65	UTSW	7	84,596,802 (GRCm39)	missense	probably damaging	0.99
R5638:Vmn2r65	UTSW	7	84,590,047 (GRCm39)	missense	probably damaging	1.00
R5673:Vmn2r65	UTSW	7	84,596,615 (GRCm39)	missense	probably benign	0.23
R5688:Vmn2r65	UTSW	7	84,589,900 (GRCm39)	missense	probably benign	0.00
R5935:Vmn2r65	UTSW	7	84,592,869 (GRCm39)	missense	probably benign	0.00
R6354:Vmn2r65	UTSW	7	84,589,574 (GRCm39)	missense	probably benign	0.35
R6372:Vmn2r65	UTSW	7	84,589,861 (GRCm39)	missense	probably damaging	1.00
R6924:Vmn2r65	UTSW	7	84,613,198 (GRCm39)	missense	probably benign	0.20
R7021:Vmn2r65	UTSW	7	84,596,587 (GRCm39)	missense	probably benign	0.00
R7195:Vmn2r65	UTSW	7	84,592,347 (GRCm39)	critical splice donor site	probably null
R7422:Vmn2r65	UTSW	7	84,595,569 (GRCm39)	missense	probably damaging	0.99
R7654:Vmn2r65	UTSW	7	84,590,261 (GRCm39)	missense	probably damaging	1.00
R7686:Vmn2r65	UTSW	7	84,589,744 (GRCm39)	missense	probably damaging	0.99
R7691:Vmn2r65	UTSW	7	84,592,851 (GRCm39)	missense	probably benign	0.30
R7798:Vmn2r65	UTSW	7	84,596,192 (GRCm39)	missense	probably damaging	1.00
R7798:Vmn2r65	UTSW	7	84,595,530 (GRCm39)	missense	probably benign	0.00
R8103:Vmn2r65	UTSW	7	84,595,919 (GRCm39)	missense	probably damaging	1.00
R8272:Vmn2r65	UTSW	7	84,596,817 (GRCm39)	missense	probably benign	0.02
R8303:Vmn2r65	UTSW	7	84,589,391 (GRCm39)	nonsense	probably null
R8354:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8454:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8489:Vmn2r65	UTSW	7	84,589,964 (GRCm39)	missense	possibly damaging	0.85
R8554:Vmn2r65	UTSW	7	84,595,960 (GRCm39)	missense	probably benign	0.00
R8680:Vmn2r65	UTSW	7	84,589,388 (GRCm39)	missense	probably benign	0.00
R8731:Vmn2r65	UTSW	7	84,589,447 (GRCm39)	nonsense	probably null
R8839:Vmn2r65	UTSW	7	84,595,489 (GRCm39)	nonsense	probably null
R8847:Vmn2r65	UTSW	7	84,590,212 (GRCm39)	missense	probably damaging	1.00
R8916:Vmn2r65	UTSW	7	84,595,665 (GRCm39)	missense	probably benign	0.21
R9254:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9379:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9392:Vmn2r65	UTSW	7	84,589,718 (GRCm39)	missense	possibly damaging	0.95
R9686:Vmn2r65	UTSW	7	84,590,084 (GRCm39)	missense	probably benign	0.45
X0067:Vmn2r65	UTSW	7	84,590,113 (GRCm39)	missense	probably benign	0.04
Z1088:Vmn2r65	UTSW	7	84,592,473 (GRCm39)	critical splice acceptor site	probably null
Z1177:Vmn2r65	UTSW	7	84,590,234 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GTAACCCAGTACACAGTAGAATGC -3'
(R):5'- TTGTCAAGGCGAATAACCGAC -3'

Sequencing Primer
(F):5'- GCAATAACTGAACCCTTGTTGCAG -3'
(R):5'- GGCGAATAACCGACTTCTCAG -3'

Posted On

2022-08-09