Mutagenetix > Incidental Mutation

Incidental Mutation 'R9568:Gfral'

721769

Institutional Source

Beutler Lab

Gene Symbol

Gfral

Ensembl Gene

ENSMUSG00000059383

Gene Name

GDNF family receptor alpha like

Synonyms

GRAL

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R9568 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

76071389-76120939 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 76104383 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 210 (C210S)

Ref Sequence

ENSEMBL: ENSMUSP00000139120 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074880] [ENSMUST00000184693]

AlphaFold

Q6SJE0

Predicted Effect

probably damaging
Transcript: ENSMUST00000074880
AA Change: C210S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000074421
Gene: ENSMUSG00000059383
AA Change: C210S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
GDNF	24	99	4.05e0	SMART
GDNF	131	210	1.15e-19	SMART
GDNF	220	316	3.15e-17	SMART
transmembrane domain	351	370	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000184693
AA Change: C210S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139120
Gene: ENSMUSG00000059383
AA Change: C210S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
GDNF	24	99	4.05e0	SMART
GDNF	131	210	1.15e-19	SMART

Meta Mutation Damage Score

0.8800

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous knockout leads to increased susceptibility to diet-induced obesity caused by overeating and reduced glucose tolerance. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam6a	T	G	12: 113,508,020 (GRCm39)	V131G	possibly damaging	Het
Agr3	G	T	12: 35,998,349 (GRCm39)	M149I	probably benign	Het
Alox12b	T	A	11: 69,054,836 (GRCm39)	C280S	possibly damaging	Het
Amhr2	C	A	15: 102,353,954 (GRCm39)	Q16K	probably benign	Het
Ank1	A	G	8: 23,609,381 (GRCm39)	T1387A	probably benign	Het
B3gnt9	A	C	8: 105,980,203 (GRCm39)	F395C	probably damaging	Het
BC048562	A	G	9: 108,322,905 (GRCm39)	D80G	probably damaging	Het
Ccdc65	T	A	15: 98,620,819 (GRCm39)	M438K	possibly damaging	Het
Ccz1	T	C	5: 143,938,251 (GRCm39)	R240G	probably damaging	Het
Cish	A	T	9: 107,177,593 (GRCm39)	S90C	probably benign	Het
Col5a2	A	T	1: 45,430,998 (GRCm39)	S881T	possibly damaging	Het
Col6a6	G	A	9: 105,657,926 (GRCm39)	T762I	possibly damaging	Het
Cpa1	A	G	6: 30,640,060 (GRCm39)	K49E	probably benign	Het
Cpb1	T	C	3: 20,320,697 (GRCm39)		probably null	Het
Csmd3	A	G	15: 48,150,942 (GRCm39)	S483P	probably damaging	Het
Cul9	G	A	17: 46,831,044 (GRCm39)	T1643I	possibly damaging	Het
Dock2	T	C	11: 34,599,638 (GRCm39)	K314E	possibly damaging	Het
Elp1	G	T	4: 56,786,711 (GRCm39)	P411T	probably damaging	Het
Fam186b	T	G	15: 99,176,571 (GRCm39)	K773T	probably damaging	Het
Fancl	T	A	11: 26,418,672 (GRCm39)	V259E	probably benign	Het
Fat4	T	G	3: 38,946,156 (GRCm39)	I1683S	probably damaging	Het
Fcamr	T	A	1: 130,732,356 (GRCm39)	H114Q	probably damaging	Het
Fndc11	T	C	2: 180,864,046 (GRCm39)	F284L	probably damaging	Het
Galns	A	G	8: 123,311,649 (GRCm39)		probably null	Het
Gch1	A	G	14: 47,426,637 (GRCm39)	S30P	probably benign	Het
Gcnt3	A	G	9: 69,942,346 (GRCm39)	I74T	probably benign	Het
Gm26566	G	A	4: 88,640,247 (GRCm39)	A13T	unknown	Het
Gm9936	A	T	5: 114,995,166 (GRCm39)	D150E	unknown	Het
Gsn	C	A	2: 35,174,003 (GRCm39)	D75E	probably benign	Het
Ighd2-7	T	C	12: 113,420,344 (GRCm39)	T5A	possibly damaging	Het
Itsn1	G	T	16: 91,649,782 (GRCm39)	R152L	probably benign	Het
Klhl3	A	T	13: 58,157,126 (GRCm39)	L620Q	probably damaging	Het
Larp7-ps	G	T	4: 92,079,915 (GRCm39)	Y24*	probably null	Het
Lpl	G	A	8: 69,340,235 (GRCm39)	V77M	probably benign	Het
Lrfn3	A	T	7: 30,058,916 (GRCm39)	H436Q	probably benign	Het
Lrp1b	A	G	2: 40,569,227 (GRCm39)	C3965R		Het
Lrrc2	G	A	9: 110,799,228 (GRCm39)		probably null	Het
Manea	C	A	4: 26,340,468 (GRCm39)	D165Y	probably damaging	Het
Myom1	A	T	17: 71,394,476 (GRCm39)	Q992L	probably damaging	Het
Naip5	T	C	13: 100,359,821 (GRCm39)	T472A	probably benign	Het
Naip5	T	A	13: 100,356,338 (GRCm39)	E1092D	probably benign	Het
Nyap1	C	T	5: 137,733,394 (GRCm39)	W546*	probably null	Het
Olfml3	T	G	3: 103,644,282 (GRCm39)	Y129S	possibly damaging	Het
Or4d5	A	T	9: 40,011,864 (GRCm39)	D307E	probably benign	Het
Or5h19	C	A	16: 58,856,213 (GRCm39)	V296F	probably damaging	Het
Or6z5	A	G	7: 6,477,334 (GRCm39)	E75G	probably damaging	Het
Or9k2b	T	A	10: 130,015,814 (GRCm39)	I312F	probably benign	Het
P2rx6	A	C	16: 17,385,300 (GRCm39)		probably null	Het
Plxnb2	A	T	15: 89,045,160 (GRCm39)	Y1095*	probably null	Het
Pmepa1	G	A	2: 173,069,794 (GRCm39)	R216C	probably damaging	Het
Prodh	A	G	16: 17,902,619 (GRCm39)		probably benign	Het
Ptch1	A	G	13: 63,689,987 (GRCm39)	Y389H	probably damaging	Het
Ptgs2	G	T	1: 149,976,842 (GRCm39)		probably null	Het
Rbm14	A	G	19: 4,861,464 (GRCm39)	Y25H	probably damaging	Het
Rfc2	T	C	5: 134,622,112 (GRCm39)	L197P	probably damaging	Het
Rnf19b	G	T	4: 128,967,397 (GRCm39)	W313L	probably damaging	Het
Sh3rf1	G	T	8: 61,825,585 (GRCm39)	A527S	probably benign	Het
Slc12a1	A	G	2: 125,032,218 (GRCm39)	Y623C	probably damaging	Het
Slc25a54	T	A	3: 109,005,932 (GRCm39)	W147R	probably damaging	Het
Slc4a1	A	G	11: 102,247,680 (GRCm39)	L401P	probably damaging	Het
Slc4a7	A	G	14: 14,796,073 (GRCm38)		probably null	Het
Stab2	A	G	10: 86,699,420 (GRCm39)	Y1933H	probably damaging	Het
Supt5	A	T	7: 28,014,688 (GRCm39)	V1037E	probably damaging	Het
Sv2b	A	T	7: 74,775,428 (GRCm39)	H466Q	probably benign	Het
Tmco5	G	A	2: 116,710,730 (GRCm39)	E17K	probably damaging	Het
Tmem69	G	A	4: 116,411,972 (GRCm39)	S12F	probably damaging	Het
Trp53	C	A	11: 69,478,392 (GRCm39)	Y100*	probably null	Het
Trpm7	A	T	2: 126,664,510 (GRCm39)	H956Q	probably benign	Het
Tsnaxip1	G	A	8: 106,569,135 (GRCm39)	R495H	probably benign	Het
Vmn1r237	A	T	17: 21,534,777 (GRCm39)	I167F	probably benign	Het
Vmn1r70	A	G	7: 10,368,292 (GRCm39)	D260G	probably benign	Het
Zbtb38	G	A	9: 96,570,944 (GRCm39)	H47Y	probably damaging	Het
Zfp184	G	T	13: 22,142,897 (GRCm39)	C201F	probably benign	Het
Zfp384	T	A	6: 125,001,796 (GRCm39)	I147N	possibly damaging	Het

Other mutations in Gfral

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01391:Gfral	APN	9	76,072,107 (GRCm39)	nonsense	probably null
IGL02383:Gfral	APN	9	76,104,374 (GRCm39)	missense	probably damaging	0.97
IGL02987:Gfral	APN	9	76,104,583 (GRCm39)	missense	possibly damaging	0.82
IGL03002:Gfral	APN	9	76,104,520 (GRCm39)	missense	possibly damaging	0.61
IGL03055:Gfral	UTSW	9	76,115,831 (GRCm39)	missense	probably benign	0.00
PIT4585001:Gfral	UTSW	9	76,104,576 (GRCm39)	missense	probably damaging	1.00
R0268:Gfral	UTSW	9	76,104,383 (GRCm39)	missense	probably damaging	1.00
R0547:Gfral	UTSW	9	76,115,924 (GRCm39)	missense	probably benign	0.16
R1146:Gfral	UTSW	9	76,074,341 (GRCm39)	missense	probably benign	0.00
R1146:Gfral	UTSW	9	76,074,341 (GRCm39)	missense	probably benign	0.00
R1275:Gfral	UTSW	9	76,104,314 (GRCm39)	missense	probably damaging	1.00
R1830:Gfral	UTSW	9	76,100,485 (GRCm39)	missense	probably benign	0.01
R2249:Gfral	UTSW	9	76,100,631 (GRCm39)	missense	probably damaging	1.00
R3709:Gfral	UTSW	9	76,100,725 (GRCm39)	nonsense	probably null
R4712:Gfral	UTSW	9	76,100,727 (GRCm39)	missense	possibly damaging	0.71
R5567:Gfral	UTSW	9	76,115,900 (GRCm39)	missense	probably benign	0.00
R5568:Gfral	UTSW	9	76,072,087 (GRCm39)	makesense	probably null
R5719:Gfral	UTSW	9	76,104,328 (GRCm39)	missense	probably benign	0.02
R5789:Gfral	UTSW	9	76,104,328 (GRCm39)	missense	probably benign	0.02
R5791:Gfral	UTSW	9	76,104,328 (GRCm39)	missense	probably benign	0.02
R7110:Gfral	UTSW	9	76,072,112 (GRCm39)	missense	possibly damaging	0.84
R7549:Gfral	UTSW	9	76,106,257 (GRCm39)	missense	probably benign	0.14
R7782:Gfral	UTSW	9	76,100,572 (GRCm39)	missense	probably benign	0.43
R7851:Gfral	UTSW	9	76,112,737 (GRCm39)	missense	probably benign	0.03
R9160:Gfral	UTSW	9	76,104,372 (GRCm39)	missense	possibly damaging	0.83
Z1177:Gfral	UTSW	9	76,112,671 (GRCm39)	missense	probably benign	0.26

Predicted Primers

PCR Primer
(F):5'- GGTGCCACAACACATGTACC -3'
(R):5'- GCATGCTCAGCAAATGGAAATC -3'

Sequencing Primer
(F):5'- CTATTTACCCTATTTTGGATGACGG -3'
(R):5'- ATCTGTGTGATGTGAAACACTGCC -3'

Posted On

2022-08-09