Mutagenetix > Incidental Mutation

Incidental Mutation 'R9575:Zfp976'

722239

Institutional Source

Beutler Lab

Gene Symbol

Zfp976

Ensembl Gene

ENSMUSG00000074158

Gene Name

zinc finger protein 976

Synonyms

9830147E19Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R9575 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

42609526-42642588 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 42612617 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 600 (T600A)

Ref Sequence

ENSEMBL: ENSMUSP00000096105 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098503] [ENSMUST00000187616]

AlphaFold

E9Q981

Predicted Effect

unknown
Transcript: ENSMUST00000098503
AA Change: T600A

SMART Domains

Protein: ENSMUSP00000096105
Gene: ENSMUSG00000074158
AA Change: T600A

Domain	Start	End	E-Value	Type
KRAB	4	66	1.73e-18	SMART
ZnF_C2H2	131	153	7.78e-3	SMART
ZnF_C2H2	159	181	1.67e-2	SMART
ZnF_C2H2	187	209	5.9e-3	SMART
ZnF_C2H2	215	237	1.38e-3	SMART
ZnF_C2H2	243	265	8.94e-3	SMART
ZnF_C2H2	271	293	2.24e-3	SMART
ZnF_C2H2	299	321	1.03e-2	SMART
ZnF_C2H2	327	349	1.58e-3	SMART
ZnF_C2H2	355	377	7.9e-4	SMART
ZnF_C2H2	383	405	5.9e-3	SMART
ZnF_C2H2	411	433	2.57e-3	SMART
ZnF_C2H2	439	461	3.16e-3	SMART
ZnF_C2H2	467	489	1.26e-2	SMART
ZnF_C2H2	495	517	8.34e-3	SMART
ZnF_C2H2	523	545	3.63e-3	SMART
ZnF_C2H2	551	573	4.79e-3	SMART
ZnF_C2H2	579	601	9.73e-4	SMART
ZnF_C2H2	607	629	3.63e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187616
AA Change: T599A

PolyPhen 2 Score 0.045 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000141023
Gene: ENSMUSG00000074158
AA Change: T599A

Domain	Start	End	E-Value	Type
KRAB	3	65	7.4e-21	SMART
ZnF_C2H2	130	152	3.2e-5	SMART
ZnF_C2H2	158	180	7.1e-5	SMART
ZnF_C2H2	186	208	2.4e-5	SMART
ZnF_C2H2	214	236	5.7e-6	SMART
ZnF_C2H2	242	264	3.8e-5	SMART
ZnF_C2H2	270	292	9.7e-6	SMART
ZnF_C2H2	298	320	4.2e-5	SMART
ZnF_C2H2	326	348	6.6e-6	SMART
ZnF_C2H2	354	376	3.3e-6	SMART
ZnF_C2H2	382	404	2.5e-5	SMART
ZnF_C2H2	410	432	1e-5	SMART
ZnF_C2H2	438	460	1.3e-5	SMART
ZnF_C2H2	466	488	5.2e-5	SMART
ZnF_C2H2	494	516	3.6e-5	SMART
ZnF_C2H2	522	544	1.6e-5	SMART
ZnF_C2H2	550	572	2e-5	SMART
ZnF_C2H2	578	600	4e-6	SMART
ZnF_C2H2	606	628	1.5e-5	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 27 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
B4galt4	T	C	16: 38,763,151	Y253H	probably damaging	Het
C330027C09Rik	T	A	16: 49,018,391	M857K	probably benign	Het
Catsperd	A	G	17: 56,628,231	R15G	unknown	Het
Cep350	G	A	1: 155,875,367	P2020S	probably benign	Het
Fancm	T	A	12: 65,105,540	D923E	possibly damaging	Het
Fastk	A	G	5: 24,445,069	S27P	probably benign	Het
Flnc	A	G	6: 29,454,400	H1937R	probably damaging	Het
Gfm2	T	C	13: 97,149,398	W132R	probably damaging	Het
Gm21119	A	T	8: 20,619,074	D148V	probably damaging	Het
Grhl1	T	C	12: 24,586,083	I348T	probably damaging	Het
Gstcd	G	T	3: 132,998,947	H515Q	probably damaging	Het
Igsf3	A	G	3: 101,431,309	Y313C	probably damaging	Het
Mbtd1	G	A	11: 93,908,938		probably null	Het
Myo9a	T	C	9: 59,905,907	S2130P	probably damaging	Het
Olfr139	A	T	11: 74,045,014	C87S	probably benign	Het
Plpp4	T	A	7: 129,323,487	F149I	probably benign	Het
Poglut1	T	C	16: 38,542,923	T165A	probably benign	Het
Rhbdf1	A	G	11: 32,213,101	I425T	probably benign	Het
Sf3a2	ACTCCAGGGGTGCACCCACCAGCTCCAGGGGTGCACCCACCAGCTCCAGGGGTGCACCCACCAGCTCCAGGGGT	ACTCCAGGGGTGCACCCACCAGCTCCAGGGGTGCACCCACCAGCTCCAGGGGT	10: 80,804,437		probably benign	Het
Slc6a9	T	C	4: 117,857,406	S175P	probably benign	Het
Slitrk3	C	T	3: 73,048,794	G882S	probably benign	Het
Spag9	A	G	11: 94,071,583	I356M	probably damaging	Het
Spef2	A	T	15: 9,596,586	L1459Q	probably damaging	Het
Tenm3	A	T	8: 48,235,761	F2264I	possibly damaging	Het
Vmn2r3	T	C	3: 64,271,314	N510S	probably benign	Het
Zfp866	A	T	8: 69,766,638	C111S	probably damaging	Het
Zfp971	T	A	2: 178,033,510	C301S	probably damaging	Het

Other mutations in Zfp976

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Zfp976	APN	7	42613685	missense	unknown
IGL01102:Zfp976	APN	7	42613909	nonsense	probably null
IGL01111:Zfp976	APN	7	42616287	missense	probably damaging	0.99
IGL01628:Zfp976	APN	7	42612511	missense	unknown
IGL02008:Zfp976	APN	7	42614232	splice site	probably benign
IGL02548:Zfp976	APN	7	42612529	missense	unknown
R0190:Zfp976	UTSW	7	42642524	start gained	probably benign
R0685:Zfp976	UTSW	7	42613717	missense	probably damaging	0.98
R1310:Zfp976	UTSW	7	42613186	missense	probably damaging	1.00
R1353:Zfp976	UTSW	7	42616018	missense	probably damaging	0.99
R1447:Zfp976	UTSW	7	42612599	missense	possibly damaging	0.79
R1569:Zfp976	UTSW	7	42613382	missense	probably damaging	1.00
R1702:Zfp976	UTSW	7	42616000	missense	possibly damaging	0.86
R1829:Zfp976	UTSW	7	42616311	missense	probably damaging	1.00
R1939:Zfp976	UTSW	7	42613681	missense	unknown
R1978:Zfp976	UTSW	7	42613841	missense	probably damaging	1.00
R1981:Zfp976	UTSW	7	42613622	missense	probably damaging	0.99
R2160:Zfp976	UTSW	7	42613930	missense	probably benign
R2192:Zfp976	UTSW	7	42613271	missense	probably damaging	1.00
R3121:Zfp976	UTSW	7	42613514	missense	probably damaging	1.00
R4210:Zfp976	UTSW	7	42616325	missense	probably damaging	0.99
R4724:Zfp976	UTSW	7	42613033	missense	possibly damaging	0.91
R4943:Zfp976	UTSW	7	42612422	unclassified	probably benign
R5047:Zfp976	UTSW	7	42613419	nonsense	probably null
R5071:Zfp976	UTSW	7	42612930	nonsense	probably null
R5125:Zfp976	UTSW	7	42612501	splice site	probably null
R5178:Zfp976	UTSW	7	42612501	splice site	probably null
R5305:Zfp976	UTSW	7	42613478	missense	probably benign	0.00
R5777:Zfp976	UTSW	7	42614080	missense	probably benign	0.00
R6153:Zfp976	UTSW	7	42614186	missense	probably damaging	0.99
R6694:Zfp976	UTSW	7	42614186	missense	probably damaging	0.99
R7226:Zfp976	UTSW	7	42613260	nonsense	probably null
R7479:Zfp976	UTSW	7	42613179	missense	probably benign	0.01
R7561:Zfp976	UTSW	7	42616277	missense	probably damaging	1.00
R8178:Zfp976	UTSW	7	42613535	missense	probably benign	0.03
R8261:Zfp976	UTSW	7	42612701	missense	unknown
R8715:Zfp976	UTSW	7	42613445	missense	possibly damaging	0.89
R8921:Zfp976	UTSW	7	42613151	missense	possibly damaging	0.57
R9168:Zfp976	UTSW	7	42613587	nonsense	probably null
Z1088:Zfp976	UTSW	7	42612760	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- ACTCCGGTGTGAATAGGCTTCA -3'
(R):5'- TTGCAATCCCACTATCTTCGAATG -3'

Sequencing Primer
(F):5'- CGGTGTGAATAGGCTTCATCACAC -3'
(R):5'- CCAGCTATCTTCGAATGCATGAAAG -3'

Posted On

2022-08-09