Mutagenetix > Incidental Mutation

Incidental Mutation 'R9580:Zfp97'

722511

Institutional Source

Beutler Lab

Gene Symbol

Zfp97

Ensembl Gene

ENSMUSG00000095990

Gene Name

zinc finger protein 97

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.152) question?

Stock #

R9580 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

17341677-17367093 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 17365243 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 247 (F247L)

Ref Sequence

ENSEMBL: ENSMUSP00000114301 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000147630]

AlphaFold

E9PZP6

Predicted Effect

probably damaging
Transcript: ENSMUST00000147630
AA Change: F247L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000114301
Gene: ENSMUSG00000095990
AA Change: F247L

Domain	Start	End	E-Value	Type
KRAB	13	82	4.42e-16	SMART
ZnF_C2H2	158	180	6.88e-4	SMART
ZnF_C2H2	186	208	4.4e-2	SMART
ZnF_C2H2	236	260	4.57e0	SMART
ZnF_C2H2	266	288	6.32e-3	SMART
ZnF_C2H2	294	316	8.94e-3	SMART
ZnF_C2H2	322	344	2.53e-2	SMART
ZnF_C2H2	350	372	9.73e-4	SMART
ZnF_C2H2	378	400	3.89e-3	SMART
ZnF_C2H2	406	428	8.94e-3	SMART
ZnF_C2H2	434	456	1.18e-2	SMART
ZnF_C2H2	462	484	7.9e-4	SMART
ZnF_C2H2	490	512	7.78e-3	SMART
ZnF_C2H2	518	540	3.95e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adora2b	T	C	11: 62,156,145 (GRCm39)	M198T	probably damaging	Het
Agbl2	A	G	2: 90,636,248 (GRCm39)	T595A	probably benign	Het
Cep89	A	T	7: 35,102,538 (GRCm39)	I84F	possibly damaging	Het
Eda	G	T	X: 99,019,775 (GRCm39)	R130L	unknown	Het
Erp44	T	A	4: 48,218,187 (GRCm39)	R161*	probably null	Het
Fcsk	A	G	8: 111,616,813 (GRCm39)	W446R	probably damaging	Het
Gm12185	T	G	11: 48,799,192 (GRCm39)	S434R	possibly damaging	Het
Hmcn2	G	T	2: 31,294,875 (GRCm39)	A2555S	probably benign	Het
Idh2	TCCCAGG	T	7: 79,748,079 (GRCm39)		probably benign	Het
Ighv1-61	A	G	12: 115,322,993 (GRCm39)	S40P	possibly damaging	Het
Kif26b	C	T	1: 178,506,643 (GRCm39)	Q240*	probably null	Het
Mapkap1	C	T	2: 34,509,878 (GRCm39)	T456M	probably damaging	Het
Ncapg2	A	G	12: 116,424,228 (GRCm39)	N1137S	probably damaging	Het
Nectin4	C	G	1: 171,211,324 (GRCm39)	R283G	probably damaging	Het
Nos2	A	G	11: 78,828,457 (GRCm39)	I259V	probably benign	Het
Or2n1d	A	T	17: 38,646,320 (GRCm39)	T91S	possibly damaging	Het
Or4c119	T	C	2: 88,987,465 (GRCm39)	N18S	probably benign	Het
Pgm2	A	T	5: 64,265,092 (GRCm39)	N371I	possibly damaging	Het
Ppargc1a	A	T	5: 51,620,139 (GRCm39)	N733K	unknown	Het
Ptpn5	A	G	7: 46,732,622 (GRCm39)	Y345H	probably damaging	Het
Ptprd	C	G	4: 75,872,315 (GRCm39)	A1134P	possibly damaging	Het
Rbm47	A	C	5: 66,183,877 (GRCm39)	M242R	possibly damaging	Het
Slc12a5	T	G	2: 164,816,896 (GRCm39)	F140V	probably damaging	Het
Slc22a2	G	T	17: 12,803,177 (GRCm39)	V4L	probably benign	Het
Stk26	C	T	X: 49,930,618 (GRCm39)		probably benign	Het
Tas2r105	A	T	6: 131,663,699 (GRCm39)	I243K	probably damaging	Het
Tmem126b	G	T	7: 90,118,231 (GRCm39)	Y216*	probably null	Het
Usp25	C	T	16: 76,880,682 (GRCm39)	T681I	probably benign	Het
Vmn1r206	C	T	13: 22,804,890 (GRCm39)	A106T	probably damaging	Het
Vmn1r39	A	T	6: 66,781,915 (GRCm39)	N134K	probably damaging	Het
Vmn1r9	T	C	6: 57,048,812 (GRCm39)	S296P	probably benign	Het

Other mutations in Zfp97

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0812:Zfp97	UTSW	17	17,365,552 (GRCm39)	missense	possibly damaging	0.77
R1550:Zfp97	UTSW	17	17,365,468 (GRCm39)	nonsense	probably null
R1920:Zfp97	UTSW	17	17,365,265 (GRCm39)	missense	probably benign	0.00
R2058:Zfp97	UTSW	17	17,365,018 (GRCm39)	missense	possibly damaging	0.57
R4896:Zfp97	UTSW	17	17,365,038 (GRCm39)	missense	probably benign	0.09
R4967:Zfp97	UTSW	17	17,364,938 (GRCm39)	nonsense	probably null
R4967:Zfp97	UTSW	17	17,365,393 (GRCm39)	missense	probably damaging	1.00
R5341:Zfp97	UTSW	17	17,365,472 (GRCm39)	missense	probably damaging	1.00
R5714:Zfp97	UTSW	17	17,365,871 (GRCm39)	missense	possibly damaging	0.86
R5866:Zfp97	UTSW	17	17,365,087 (GRCm39)	missense	possibly damaging	0.79
R6861:Zfp97	UTSW	17	17,365,437 (GRCm39)	missense	probably damaging	1.00
R7506:Zfp97	UTSW	17	17,365,542 (GRCm39)	missense	probably damaging	1.00
R9136:Zfp97	UTSW	17	17,365,215 (GRCm39)	missense	probably benign	0.00
R9516:Zfp97	UTSW	17	17,365,930 (GRCm39)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- AGGACACTGGATCTTTCATGTC -3'
(R):5'- GCTCTGCCACACTGGTTACA -3'

Sequencing Primer
(F):5'- TCAATGTGGGAAAGCCTTTGCAC -3'
(R):5'- GCCACACTGGTTACATTCATAGGG -3'

Posted On

2022-08-09