Mutagenetix > Incidental Mutation

Incidental Mutation 'R9585:Ddx25'

722752

Institutional Source

Beutler Lab

Gene Symbol

Ddx25

Ensembl Gene

ENSMUSG00000032101

Gene Name

DEAD box helicase 25

Synonyms

DEAD (Asp-Glu-Ala-Asp) box polypeptide 25, GRTH

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.318) question?

Stock #

R9585 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

35453144-35469766 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 35455009 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 426 (Y426*)

Ref Sequence

ENSEMBL: ENSMUSP00000034612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034612]

AlphaFold

Q9QY15

Predicted Effect

probably null
Transcript: ENSMUST00000034612
AA Change: Y426*

SMART Domains

Protein: ENSMUSP00000034612
Gene: ENSMUSG00000032101
AA Change: Y426*

Domain	Start	End	E-Value	Type
low complexity region	50	61	N/A	INTRINSIC
low complexity region	101	111	N/A	INTRINSIC
DEXDc	117	316	1.26e-41	SMART
HELICc	353	440	6.18e-27	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice display male infertility, arrest of spermatogenesis at step 8, abnormal Leydig cells, and increased germ cell apoptosis in males. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	T	A	3: 124,199,993 (GRCm39)	D533V	possibly damaging	Het
4933411K16Rik	A	G	19: 42,041,352 (GRCm39)	E161G	probably benign	Het
Abca12	A	G	1: 71,342,745 (GRCm39)	S912P	probably damaging	Het
Abca3	G	A	17: 24,619,486 (GRCm39)	M1196I	probably benign	Het
Adprs	A	T	4: 126,211,786 (GRCm39)	D175E	probably benign	Het
Asrgl1	A	G	19: 9,090,398 (GRCm39)	L316P	probably benign	Het
Avl9	T	A	6: 56,734,299 (GRCm39)	M626K	probably damaging	Het
Birc6	A	G	17: 74,916,265 (GRCm39)	N1727S	probably damaging	Het
Casp14	G	A	10: 78,549,194 (GRCm39)	R251W	probably damaging	Het
Cfap69	A	G	5: 5,631,269 (GRCm39)	I919T	possibly damaging	Het
Cibar2	T	C	8: 120,901,450 (GRCm39)	E85G	probably null	Het
Cps1	T	A	1: 67,195,341 (GRCm39)	M254K	probably damaging	Het
Ctc1	A	G	11: 68,925,490 (GRCm39)	E1009G	probably damaging	Het
Dok3	A	G	13: 55,672,057 (GRCm39)	F207S	probably damaging	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Fcrla	A	T	1: 170,749,868 (GRCm39)	M1K	probably null	Het
Heatr4	A	T	12: 84,014,472 (GRCm39)	S588R	probably damaging	Het
Iglv3	A	G	16: 19,059,960 (GRCm39)	*123Q	probably null	Het
Igsf9b	T	C	9: 27,233,532 (GRCm39)	I344T	probably damaging	Het
Il18	T	C	9: 50,490,661 (GRCm39)	S99P	probably damaging	Het
Krt36	G	A	11: 99,994,892 (GRCm39)	L227F	probably damaging	Het
Lrriq1	A	G	10: 103,051,250 (GRCm39)	S501P	probably benign	Het
Lvrn	T	C	18: 47,011,411 (GRCm39)		probably null	Het
Myo18a	T	A	11: 77,709,495 (GRCm39)	M535K	probably benign	Het
Myocd	T	A	11: 65,095,192 (GRCm39)	S158C	probably damaging	Het
Naip6	A	T	13: 100,436,577 (GRCm39)	C649S	probably damaging	Het
Oasl2	T	C	5: 115,035,901 (GRCm39)	V59A	probably damaging	Het
Obscn	C	A	11: 58,965,831 (GRCm39)	V2942F	probably benign	Het
Or10a49	T	A	7: 108,467,552 (GRCm39)	T270S	probably benign	Het
Or5l14	T	A	2: 87,792,919 (GRCm39)	T106S	probably benign	Het
Osbpl6	A	G	2: 76,354,438 (GRCm39)	T18A	probably benign	Het
Pcf11	A	T	7: 92,311,006 (GRCm39)	D327E	probably benign	Het
Per3	G	T	4: 151,097,138 (GRCm39)	Q796K	probably benign	Het
Pex5l	T	C	3: 33,060,091 (GRCm39)	T227A	probably benign	Het
Phf11b	G	T	14: 59,568,704 (GRCm39)	P70T	probably benign	Het
Pkd1l1	T	G	11: 8,804,390 (GRCm39)	I2184L		Het
Polr3a	A	T	14: 24,502,289 (GRCm39)	M1288K	probably damaging	Het
Pth1r	T	A	9: 110,573,847 (GRCm39)	R3S	probably benign	Het
Ptprk	C	A	10: 28,369,147 (GRCm39)	Y706*	probably null	Het
Rmi2	C	T	16: 10,703,983 (GRCm39)	T108I	probably benign	Het
Rrbp1	T	C	2: 143,799,479 (GRCm39)	N1076S	probably benign	Het
Setd3	A	C	12: 108,074,814 (GRCm39)		probably null	Het
Slc4a1	A	G	11: 102,247,915 (GRCm39)	Y360H	probably benign	Het
Sox21	A	G	14: 118,472,993 (GRCm39)	S19P	possibly damaging	Het
Speer4a2	T	C	5: 26,291,542 (GRCm39)	H88R	possibly damaging	Het
Stx18	T	A	5: 38,249,916 (GRCm39)	N76K	possibly damaging	Het
Sv2c	T	C	13: 96,122,466 (GRCm39)	T437A	probably benign	Het
Trrap	G	A	5: 144,777,330 (GRCm39)	V3043M	probably damaging	Het
Vps50	T	A	6: 3,600,348 (GRCm39)	S936T	probably benign	Het

Other mutations in Ddx25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Ddx25	APN	9	35,454,891 (GRCm39)	splice site	probably benign
IGL00951:Ddx25	APN	9	35,464,131 (GRCm39)	critical splice donor site	probably null
IGL02237:Ddx25	APN	9	35,453,365 (GRCm39)	splice site	probably benign
IGL02270:Ddx25	APN	9	35,465,708 (GRCm39)	splice site	probably benign
IGL02273:Ddx25	APN	9	35,458,122 (GRCm39)	missense	possibly damaging	0.95
IGL02325:Ddx25	APN	9	35,465,804 (GRCm39)	unclassified	probably benign
IGL02422:Ddx25	APN	9	35,462,660 (GRCm39)	missense	probably null	1.00
IGL02440:Ddx25	APN	9	35,468,974 (GRCm39)	unclassified	probably benign
IGL02798:Ddx25	APN	9	35,462,693 (GRCm39)	missense	probably damaging	1.00
IGL03339:Ddx25	APN	9	35,453,299 (GRCm39)	missense	probably damaging	1.00
R0633:Ddx25	UTSW	9	35,457,268 (GRCm39)	missense	probably damaging	0.99
R0893:Ddx25	UTSW	9	35,465,686 (GRCm39)	nonsense	probably null
R1171:Ddx25	UTSW	9	35,458,142 (GRCm39)	nonsense	probably null
R1448:Ddx25	UTSW	9	35,469,034 (GRCm39)	missense	probably benign
R1453:Ddx25	UTSW	9	35,453,298 (GRCm39)	missense	probably damaging	1.00
R1582:Ddx25	UTSW	9	35,457,272 (GRCm39)	missense	probably damaging	0.97
R3055:Ddx25	UTSW	9	35,462,647 (GRCm39)	missense	probably damaging	1.00
R5960:Ddx25	UTSW	9	35,465,807 (GRCm39)	splice site	probably null
R7425:Ddx25	UTSW	9	35,465,882 (GRCm39)	missense	probably benign	0.08
R7535:Ddx25	UTSW	9	35,454,951 (GRCm39)	missense	possibly damaging	0.89
R7610:Ddx25	UTSW	9	35,465,893 (GRCm39)	missense	possibly damaging	0.90
R8758:Ddx25	UTSW	9	35,453,300 (GRCm39)	missense	probably benign
R8931:Ddx25	UTSW	9	35,465,864 (GRCm39)	missense	possibly damaging	0.85
R8984:Ddx25	UTSW	9	35,468,685 (GRCm39)	missense	probably benign
R9103:Ddx25	UTSW	9	35,458,085 (GRCm39)	missense	probably benign	0.24
R9759:Ddx25	UTSW	9	35,457,265 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCATGGCTTCTGCGTCACAC -3'
(R):5'- CACGGTGTGTAAGCAAACTGAC -3'

Sequencing Primer
(F):5'- GCTTCTGCGTCACACAACCC -3'
(R):5'- GACATAAAACCAGTTTTGATTTCCAG -3'

Posted On

2022-08-09