Mutagenetix > Incidental Mutation

Incidental Mutation 'R9586:Vmn2r45'

722797

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r45

Ensembl Gene

ENSMUSG00000090662

Gene Name

vomeronasal 2, receptor 45

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R9586 (G1)

Quality Score

151.008

Status

Not validated

Chromosome

Chromosomal Location

8474468-8491958 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 8486050 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 413 (N413D)

Ref Sequence

ENSEMBL: ENSMUSP00000129466 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164845]

AlphaFold

L7N2B5

Predicted Effect

probably benign
Transcript: ENSMUST00000164845
AA Change: N413D

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000129466
Gene: ENSMUSG00000090662
AA Change: N413D

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	469	4.5e-26	PFAM
Pfam:NCD3G	512	565	6.4e-21	PFAM
Pfam:7tm_3	598	833	2.1e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arl6ip5	C	T	6: 97,206,572 (GRCm39)	A84V	possibly damaging	Het
Cep170	G	A	1: 176,563,463 (GRCm39)	P716L	possibly damaging	Het
Chrm3	A	T	13: 9,927,480 (GRCm39)	C519S	probably damaging	Het
Dnase1l3	A	T	14: 7,967,796 (GRCm38)	V299D	possibly damaging	Het
Dync1h1	T	C	12: 110,582,975 (GRCm39)	L386P	probably damaging	Het
Elmo3	A	G	8: 106,034,760 (GRCm39)	Y382C	probably damaging	Het
Eprs1	T	C	1: 185,139,746 (GRCm39)	L945P		Het
Fstl4	A	T	11: 53,077,729 (GRCm39)	I829L	probably benign	Het
Gm36210	T	A	7: 4,909,324 (GRCm39)	R45*	probably null	Het
Homer2	C	T	7: 81,260,113 (GRCm39)	R318Q	probably benign	Het
Ice2	G	A	9: 69,314,478 (GRCm39)	E76K	probably damaging	Het
Ifi203	T	A	1: 173,754,623 (GRCm39)	R703*	probably null	Het
Insyn2a	T	C	7: 134,520,180 (GRCm39)	T117A	probably damaging	Het
Lonp1	G	A	17: 56,924,839 (GRCm39)	R523C	probably damaging	Het
Myh10	G	A	11: 68,703,820 (GRCm39)	V1881I	possibly damaging	Het
Nfe2	T	C	15: 103,157,244 (GRCm39)	D249G	probably damaging	Het
Nvl	A	T	1: 180,932,635 (GRCm39)		probably null	Het
Oog3	A	T	4: 143,884,966 (GRCm39)	C323*	probably null	Het
Ovgp1	A	G	3: 105,881,138 (GRCm39)	R3G	probably damaging	Het
Pex1	A	G	5: 3,676,047 (GRCm39)	I838M	probably damaging	Het
Rps6kc1	C	A	1: 190,514,774 (GRCm39)	M1006I	probably benign	Het
Rpusd1	A	G	17: 25,947,292 (GRCm39)	T37A	probably damaging	Het
Sema6d	C	A	2: 124,496,096 (GRCm39)	R47S	probably damaging	Het
Serpini2	G	A	3: 75,166,891 (GRCm39)	T122I	probably benign	Het
Slc10a7	A	G	8: 79,456,266 (GRCm39)	I289M	probably damaging	Het
Slc25a37	G	T	14: 69,482,421 (GRCm39)	N293K	probably benign	Het
Sspo	G	A	6: 48,458,039 (GRCm39)	A3281T	probably benign	Het
Stard13	A	T	5: 150,985,832 (GRCm39)	N559K	possibly damaging	Het
Tmem87b	T	A	2: 128,660,260 (GRCm39)	M1K	probably null	Het
Vmn1r199	C	T	13: 22,567,716 (GRCm39)	R337C	probably benign	Het
Vmn1r55	T	A	7: 5,149,770 (GRCm39)	N218Y	probably benign	Het
Vmn2r41	T	G	7: 8,141,742 (GRCm39)	Q574P		Het
Wdr33	T	C	18: 31,966,669 (GRCm39)	S170P	probably damaging	Het
Zfp385a	A	T	15: 103,241,580 (GRCm39)	I20N	probably damaging	Het
Zfp493	A	G	13: 67,932,017 (GRCm39)	S39G	possibly damaging	Het

Other mutations in Vmn2r45

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01287:Vmn2r45	APN	7	8,488,622 (GRCm39)	missense	probably benign	0.00
IGL01322:Vmn2r45	APN	7	8,484,332 (GRCm39)	missense	possibly damaging	0.49
IGL01402:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01404:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01529:Vmn2r45	APN	7	8,486,493 (GRCm39)	missense	probably benign
IGL01596:Vmn2r45	APN	7	8,486,272 (GRCm39)	missense	probably damaging	0.98
IGL01646:Vmn2r45	APN	7	8,486,337 (GRCm39)	missense	probably benign	0.18
IGL01819:Vmn2r45	APN	7	8,488,556 (GRCm39)	missense	probably benign	0.00
IGL02096:Vmn2r45	APN	7	8,484,443 (GRCm39)	missense	probably damaging	0.99
IGL02130:Vmn2r45	APN	7	8,486,556 (GRCm39)	missense	possibly damaging	0.46
IGL02409:Vmn2r45	APN	7	8,488,727 (GRCm39)	missense	probably benign	0.03
IGL02517:Vmn2r45	APN	7	8,486,185 (GRCm39)	nonsense	probably null
IGL02633:Vmn2r45	APN	7	8,488,728 (GRCm39)	missense	probably benign	0.01
IGL02940:Vmn2r45	APN	7	8,475,369 (GRCm39)	missense	probably damaging	0.99
IGL03281:Vmn2r45	APN	7	8,486,603 (GRCm39)	missense	probably damaging	1.00
IGL03358:Vmn2r45	APN	7	8,474,715 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
BB014:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R0382:Vmn2r45	UTSW	7	8,486,098 (GRCm39)	missense	probably benign	0.06
R0532:Vmn2r45	UTSW	7	8,474,820 (GRCm39)	missense	probably damaging	0.99
R0932:Vmn2r45	UTSW	7	8,478,380 (GRCm39)	missense	probably damaging	1.00
R1125:Vmn2r45	UTSW	7	8,488,542 (GRCm39)	missense	probably benign	0.00
R1580:Vmn2r45	UTSW	7	8,474,746 (GRCm39)	missense	possibly damaging	0.95
R1817:Vmn2r45	UTSW	7	8,475,372 (GRCm39)	missense	probably damaging	1.00
R1996:Vmn2r45	UTSW	7	8,475,024 (GRCm39)	missense	probably damaging	1.00
R2050:Vmn2r45	UTSW	7	8,475,021 (GRCm39)	missense	probably damaging	1.00
R2284:Vmn2r45	UTSW	7	8,488,765 (GRCm39)	missense	probably benign	0.02
R4019:Vmn2r45	UTSW	7	8,474,580 (GRCm39)	nonsense	probably null
R4227:Vmn2r45	UTSW	7	8,486,277 (GRCm39)	missense	probably damaging	0.98
R4381:Vmn2r45	UTSW	7	8,474,912 (GRCm39)	nonsense	probably null
R4618:Vmn2r45	UTSW	7	8,486,436 (GRCm39)	missense	probably benign	0.00
R4624:Vmn2r45	UTSW	7	8,484,341 (GRCm39)	missense	probably damaging	1.00
R4704:Vmn2r45	UTSW	7	8,486,535 (GRCm39)	nonsense	probably null
R4735:Vmn2r45	UTSW	7	8,486,472 (GRCm39)	missense	probably damaging	1.00
R4868:Vmn2r45	UTSW	7	8,484,480 (GRCm39)	missense	probably benign	0.06
R4983:Vmn2r45	UTSW	7	8,486,116 (GRCm39)	missense	probably damaging	0.99
R5246:Vmn2r45	UTSW	7	8,486,251 (GRCm39)	missense	probably benign	0.00
R5430:Vmn2r45	UTSW	7	8,486,333 (GRCm39)	nonsense	probably null
R5504:Vmn2r45	UTSW	7	8,486,176 (GRCm39)	missense	probably benign	0.03
R5511:Vmn2r45	UTSW	7	8,474,832 (GRCm39)	missense	probably benign	0.19
R5745:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R5814:Vmn2r45	UTSW	7	8,474,475 (GRCm39)	missense	probably benign	0.00
R6223:Vmn2r45	UTSW	7	8,486,301 (GRCm39)	missense	probably benign
R6267:Vmn2r45	UTSW	7	8,475,207 (GRCm39)	missense	probably benign	0.26
R6623:Vmn2r45	UTSW	7	8,474,500 (GRCm39)	missense	probably benign	0.09
R6999:Vmn2r45	UTSW	7	8,486,219 (GRCm39)	missense	probably benign
R7242:Vmn2r45	UTSW	7	8,488,612 (GRCm39)	nonsense	probably null
R7491:Vmn2r45	UTSW	7	8,484,342 (GRCm39)	missense	probably benign	0.27
R7620:Vmn2r45	UTSW	7	8,486,222 (GRCm39)	nonsense	probably null
R7719:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7720:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7853:Vmn2r45	UTSW	7	8,485,987 (GRCm39)	missense	possibly damaging	0.90
R7873:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R7882:Vmn2r45	UTSW	7	8,486,409 (GRCm39)	missense	possibly damaging	0.82
R7927:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R8684:Vmn2r45	UTSW	7	8,486,511 (GRCm39)	missense	probably damaging	0.99
R8780:Vmn2r45	UTSW	7	8,484,371 (GRCm39)	missense	possibly damaging	0.94
R8811:Vmn2r45	UTSW	7	8,474,881 (GRCm39)	missense	probably damaging	1.00
R8893:Vmn2r45	UTSW	7	8,488,619 (GRCm39)	missense	probably damaging	1.00
R9043:Vmn2r45	UTSW	7	8,474,904 (GRCm39)	missense	possibly damaging	0.92
R9208:Vmn2r45	UTSW	7	8,486,298 (GRCm39)	missense	probably damaging	1.00
R9327:Vmn2r45	UTSW	7	8,474,532 (GRCm39)	missense	probably damaging	1.00
R9599:Vmn2r45	UTSW	7	8,478,457 (GRCm39)	critical splice acceptor site	probably null
U24488:Vmn2r45	UTSW	7	8,475,361 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r45	UTSW	7	8,474,484 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- TGTGTGCTCTGTATAAAACCACAC -3'
(R):5'- CATGGTTCCATCTCAGAAACACAG -3'

Sequencing Primer
(F):5'- ACCACACATTTTCATTGCTAATCTG -3'
(R):5'- ATCTAGTAATGCAAGAGTGGAAATAC -3'

Posted On

2022-08-09