Incidental Mutation 'R9589:Zfp28'
| ID |
722928 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp28
|
| Ensembl Gene |
ENSMUSG00000062861 |
| Gene Name |
zinc finger protein 28 |
| Synonyms |
2810438M17Rik, mkr-5, Zfp-28 |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.138)
|
| Stock # |
R9589 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
6386317-6399636 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 6392816 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Methionine to Lysine
at position 164
(M164K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000079812
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000081022]
|
| AlphaFold |
P10078 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000081022
AA Change: M164K
PolyPhen 2
Score 0.094 (Sensitivity: 0.93; Specificity: 0.85)
|
| SMART Domains |
Protein: ENSMUSP00000079812
Gene: ENSMUSG00000062861
AA Change: M164K
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
27 |
50 |
N/A |
INTRINSIC |
|
KRAB
|
103 |
163 |
3.53e-33 |
SMART |
|
ZnF_C2H2
|
377 |
399 |
3.95e-4 |
SMART |
|
ZnF_C2H2
|
405 |
427 |
6.88e-4 |
SMART |
|
ZnF_C2H2
|
433 |
456 |
1.2e-3 |
SMART |
|
ZnF_C2H2
|
462 |
484 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
490 |
512 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
518 |
540 |
1.82e-3 |
SMART |
|
ZnF_C2H2
|
546 |
568 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
574 |
596 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
602 |
624 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
630 |
652 |
4.87e-4 |
SMART |
|
ZnF_C2H2
|
658 |
680 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
686 |
708 |
2.36e-2 |
SMART |
|
ZnF_C2H2
|
714 |
736 |
6.42e-4 |
SMART |
|
ZnF_C2H2
|
742 |
764 |
2.4e-3 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000207086
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000207465
AA Change: M29K
PolyPhen 2
Score 0.957 (Sensitivity: 0.78; Specificity: 0.95)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000207809
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000208338
AA Change: M21K
PolyPhen 2
Score 0.957 (Sensitivity: 0.78; Specificity: 0.95)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000208949
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.9%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 36 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adgrg3 |
A |
G |
8: 95,760,093 (GRCm39) |
D104G |
possibly damaging |
Het |
| Ahnak2 |
G |
T |
12: 112,746,831 (GRCm39) |
D1166E |
|
Het |
| C2cd3 |
T |
A |
7: 100,081,756 (GRCm39) |
S1133T |
|
Het |
| Ccdc17 |
A |
G |
4: 116,454,791 (GRCm39) |
Q168R |
probably benign |
Het |
| Fn1 |
A |
T |
1: 71,668,941 (GRCm39) |
I668N |
probably damaging |
Het |
| Fyb2 |
C |
A |
4: 104,872,833 (GRCm39) |
N735K |
probably damaging |
Het |
| Gad1 |
T |
C |
2: 70,416,284 (GRCm39) |
S248P |
possibly damaging |
Het |
| Gcnt1 |
G |
A |
19: 17,307,422 (GRCm39) |
A101V |
probably benign |
Het |
| Herc1 |
T |
A |
9: 66,372,840 (GRCm39) |
M3132K |
possibly damaging |
Het |
| Htra3 |
T |
C |
5: 35,821,409 (GRCm39) |
I323V |
probably damaging |
Het |
| Ighv11-2 |
A |
T |
12: 114,012,047 (GRCm39) |
V56D |
probably damaging |
Het |
| Itga8 |
C |
T |
2: 12,237,701 (GRCm39) |
G340R |
probably damaging |
Het |
| Kcnk15 |
C |
T |
2: 163,700,127 (GRCm39) |
T122M |
probably damaging |
Het |
| Kif13a |
G |
T |
13: 46,956,020 (GRCm39) |
Q658K |
probably benign |
Het |
| Kif13b |
T |
C |
14: 65,013,759 (GRCm39) |
I1303T |
possibly damaging |
Het |
| Kifc3 |
A |
T |
8: 95,861,372 (GRCm39) |
S94R |
possibly damaging |
Het |
| Lrrc63 |
A |
T |
14: 75,322,379 (GRCm39) |
F575Y |
possibly damaging |
Het |
| Map1a |
T |
C |
2: 121,136,398 (GRCm39) |
S2405P |
probably damaging |
Het |
| Map2 |
A |
G |
1: 66,449,753 (GRCm39) |
T159A |
probably benign |
Het |
| Mgam |
A |
T |
6: 40,727,519 (GRCm39) |
I1315F |
probably damaging |
Het |
| Or51a39 |
T |
C |
7: 102,363,372 (GRCm39) |
T83A |
probably damaging |
Het |
| Pah |
T |
A |
10: 87,403,197 (GRCm39) |
F191I |
probably damaging |
Het |
| Plekhh2 |
G |
A |
17: 84,854,918 (GRCm39) |
D51N |
possibly damaging |
Het |
| Ppp4r2 |
C |
T |
6: 100,838,409 (GRCm39) |
T109M |
probably damaging |
Het |
| Rfx7 |
T |
C |
9: 72,525,122 (GRCm39) |
S771P |
possibly damaging |
Het |
| Sec11a |
T |
C |
7: 80,565,899 (GRCm39) |
*171W |
probably null |
Het |
| Secisbp2l |
C |
A |
2: 125,589,425 (GRCm39) |
V708L |
probably benign |
Het |
| Secisbp2l |
T |
G |
2: 125,589,430 (GRCm39) |
D706A |
probably damaging |
Het |
| Shpk |
C |
T |
11: 73,104,267 (GRCm39) |
P139S |
possibly damaging |
Het |
| Slc6a18 |
A |
G |
13: 73,816,323 (GRCm39) |
L352P |
possibly damaging |
Het |
| Slitrk3 |
T |
C |
3: 72,957,981 (GRCm39) |
T264A |
probably benign |
Het |
| Smg9 |
T |
G |
7: 24,120,246 (GRCm39) |
S407A |
probably damaging |
Het |
| Stmn4 |
A |
G |
14: 66,595,338 (GRCm39) |
E121G |
probably damaging |
Het |
| Tmem237 |
G |
A |
1: 59,159,146 (GRCm39) |
P10S |
probably benign |
Het |
| Vmn2r73 |
A |
G |
7: 85,519,659 (GRCm39) |
I433T |
probably benign |
Het |
| Zswim8 |
A |
G |
14: 20,763,171 (GRCm39) |
M357V |
probably damaging |
Het |
|
| Other mutations in Zfp28 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00737:Zfp28
|
APN |
7 |
6,396,429 (GRCm39) |
makesense |
probably null |
|
|
IGL02300:Zfp28
|
APN |
7 |
6,392,495 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02541:Zfp28
|
APN |
7 |
6,396,479 (GRCm39) |
nonsense |
probably null |
|
|
FR4340:Zfp28
|
UTSW |
7 |
6,397,862 (GRCm39) |
missense |
probably damaging |
1.00 |
|
FR4342:Zfp28
|
UTSW |
7 |
6,397,862 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0442:Zfp28
|
UTSW |
7 |
6,397,998 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0462:Zfp28
|
UTSW |
7 |
6,395,239 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R0799:Zfp28
|
UTSW |
7 |
6,387,182 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
R1081:Zfp28
|
UTSW |
7 |
6,392,779 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R1674:Zfp28
|
UTSW |
7 |
6,397,942 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R1783:Zfp28
|
UTSW |
7 |
6,397,791 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2119:Zfp28
|
UTSW |
7 |
6,397,875 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2186:Zfp28
|
UTSW |
7 |
6,397,497 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4280:Zfp28
|
UTSW |
7 |
6,396,700 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4281:Zfp28
|
UTSW |
7 |
6,396,700 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4283:Zfp28
|
UTSW |
7 |
6,396,700 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4331:Zfp28
|
UTSW |
7 |
6,396,700 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4379:Zfp28
|
UTSW |
7 |
6,396,441 (GRCm39) |
missense |
probably benign |
0.11 |
|
R4380:Zfp28
|
UTSW |
7 |
6,396,441 (GRCm39) |
missense |
probably benign |
0.11 |
|
R4505:Zfp28
|
UTSW |
7 |
6,397,160 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4659:Zfp28
|
UTSW |
7 |
6,396,506 (GRCm39) |
missense |
probably benign |
0.05 |
|
R4706:Zfp28
|
UTSW |
7 |
6,392,793 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5524:Zfp28
|
UTSW |
7 |
6,397,850 (GRCm39) |
splice site |
probably null |
|
|
R6269:Zfp28
|
UTSW |
7 |
6,396,612 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6981:Zfp28
|
UTSW |
7 |
6,397,692 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7117:Zfp28
|
UTSW |
7 |
6,397,461 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7176:Zfp28
|
UTSW |
7 |
6,386,456 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
R7312:Zfp28
|
UTSW |
7 |
6,386,593 (GRCm39) |
unclassified |
probably benign |
|
|
R7422:Zfp28
|
UTSW |
7 |
6,397,748 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7423:Zfp28
|
UTSW |
7 |
6,396,956 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7937:Zfp28
|
UTSW |
7 |
6,396,785 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8110:Zfp28
|
UTSW |
7 |
6,392,828 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8704:Zfp28
|
UTSW |
7 |
6,397,637 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8804:Zfp28
|
UTSW |
7 |
6,393,399 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8854:Zfp28
|
UTSW |
7 |
6,397,938 (GRCm39) |
missense |
probably benign |
0.01 |
|
R9071:Zfp28
|
UTSW |
7 |
6,397,544 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9235:Zfp28
|
UTSW |
7 |
6,397,605 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9276:Zfp28
|
UTSW |
7 |
6,397,440 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9600:Zfp28
|
UTSW |
7 |
6,397,917 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9651:Zfp28
|
UTSW |
7 |
6,395,623 (GRCm39) |
missense |
|
|
|
R9653:Zfp28
|
UTSW |
7 |
6,395,623 (GRCm39) |
missense |
|
|
|
R9712:Zfp28
|
UTSW |
7 |
6,396,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Y4340:Zfp28
|
UTSW |
7 |
6,397,656 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1177:Zfp28
|
UTSW |
7 |
6,387,186 (GRCm39) |
missense |
probably benign |
0.28 |
|
| Predicted Primers |
PCR Primer
(F):5'- CCTGTGGTAGTGGAACCTTC -3'
(R):5'- TGACTCGGTAAGACTGACAGG -3'
Sequencing Primer
(F):5'- CCGATCCCTGTAGCGCC -3'
(R):5'- CTCGGTAAGACTGACAGGGAAAG -3'
|
| Posted On |
2022-08-09 |
Incidental Mutation