Mutagenetix > Incidental Mutation

Incidental Mutation 'R9596:Or6c65'

723287

Institutional Source

Beutler Lab

Gene Symbol

Or6c65

Ensembl Gene

ENSMUSG00000049894

Gene Name

olfactory receptor family 6 subfamily C member 65

Synonyms

GA_x6K02T2PULF-11446184-11447122, Olfr808, MOR112-2

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R9596 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

129603367-129604305 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 129603988 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 208 (T208A)

Ref Sequence

ENSEMBL: ENSMUSP00000145315 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060636] [ENSMUST00000203236]

AlphaFold

Q8VGI8

Predicted Effect

probably damaging
Transcript: ENSMUST00000060636
AA Change: T208A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000059714
Gene: ENSMUSG00000049894
AA Change: T208A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	308	1.8e-53	PFAM
Pfam:7tm_1	39	288	7.7e-25	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000203236
AA Change: T208A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000145315
Gene: ENSMUSG00000049894
AA Change: T208A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	308	1.8e-53	PFAM
Pfam:7tm_1	39	288	7.7e-25	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aamp	G	T	1: 74,320,262 (GRCm39)	Q355K	probably benign	Het
Acte1	G	T	7: 143,434,902 (GRCm39)		probably null	Het
AI661453	T	C	17: 47,780,411 (GRCm39)	S188P	unknown	Het
Atp10a	A	G	7: 58,477,553 (GRCm39)	S1251G	probably damaging	Het
Ccr10	A	G	11: 101,065,018 (GRCm39)	F171L	probably benign	Het
Clec10a	A	T	11: 70,059,973 (GRCm39)	D65V	probably damaging	Het
Clybl	A	T	14: 122,548,768 (GRCm39)	I57L	probably benign	Het
Col5a1	G	T	2: 27,819,551 (GRCm39)	E218*	probably null	Het
Diaph1	G	A	18: 38,024,111 (GRCm39)	P576S	unknown	Het
Dsg1c	T	A	18: 20,416,361 (GRCm39)	I754N	probably benign	Het
Fam47e	G	C	5: 92,726,395 (GRCm39)	R111P	probably benign	Het
Fastkd3	G	A	13: 68,735,806 (GRCm39)	V519M	probably damaging	Het
Fhod3	T	A	18: 25,193,392 (GRCm39)	Y634*	probably null	Het
Frem3	G	A	8: 81,341,951 (GRCm39)	V1415I	probably benign	Het
Herc2	G	A	7: 55,834,595 (GRCm39)	S3191N		Het
Kif19a	A	T	11: 114,676,752 (GRCm39)	E527V	probably benign	Het
Lmbr1	C	A	5: 29,440,105 (GRCm39)	G420*	probably null	Het
Map3k9	A	G	12: 81,777,404 (GRCm39)	S526P	probably damaging	Het
Mink1	T	A	11: 70,497,915 (GRCm39)	L506Q	possibly damaging	Het
Mroh9	T	C	1: 162,893,576 (GRCm39)	I200V	probably damaging	Het
Mrps14	T	C	1: 160,027,122 (GRCm39)	V110A	possibly damaging	Het
Mta1	A	G	12: 113,090,470 (GRCm39)	Q209R	probably damaging	Het
Mtss2	G	A	8: 111,458,689 (GRCm39)	S195N		Het
Nlgn1	T	A	3: 25,488,587 (GRCm39)	I583F	probably damaging	Het
Ntng1	T	C	3: 110,042,956 (GRCm39)		probably benign	Het
Or10w1	A	G	19: 13,632,002 (GRCm39)	S70G	probably damaging	Het
Or1p1c	C	T	11: 74,160,289 (GRCm39)	H25Y	probably benign	Het
Or2ag13	A	T	7: 106,313,412 (GRCm39)	Y159N	probably benign	Het
Or5ac25	A	G	16: 59,181,942 (GRCm39)	I213T	possibly damaging	Het
Pcdha12	A	T	18: 37,154,302 (GRCm39)	L340F	probably benign	Het
Pdk4	T	A	6: 5,491,842 (GRCm39)	M173L	probably benign	Het
Pds5a	A	G	5: 65,772,830 (GRCm39)	S1258P	probably benign	Het
Plcd1	A	T	9: 118,917,183 (GRCm39)	L22Q	probably benign	Het
Prag1	A	G	8: 36,570,113 (GRCm39)	D232G	probably damaging	Het
Rest	C	T	5: 77,423,141 (GRCm39)	T315M	probably damaging	Het
Scrn2	G	A	11: 96,923,907 (GRCm39)	V264M	probably damaging	Het
Sipa1l3	C	A	7: 29,031,691 (GRCm39)	V1513F	probably benign	Het
Skic3	T	C	13: 76,330,968 (GRCm39)	V1466A	possibly damaging	Het
Slc44a1	T	A	4: 53,544,553 (GRCm39)	N421K	probably benign	Het
Smc6	G	T	12: 11,345,045 (GRCm39)	R662I	probably damaging	Het
Spata31e5	C	A	1: 28,815,688 (GRCm39)	M781I	probably benign	Het
Srcap	T	A	7: 127,131,036 (GRCm39)	W751R	probably damaging	Het
Sv2b	A	G	7: 74,767,462 (GRCm39)	F645L	probably damaging	Het
Sycp2	A	G	2: 177,990,212 (GRCm39)		probably null	Het
Syne4	A	G	7: 30,014,504 (GRCm39)	T41A	probably benign	Het
Tns3	G	A	11: 8,401,142 (GRCm39)	T1052M	possibly damaging	Het
Trap1	A	T	16: 3,871,374 (GRCm39)	I381N	probably damaging	Het
Tshz1	A	G	18: 84,031,904 (GRCm39)	S835P	possibly damaging	Het
Ube3b	T	A	5: 114,527,171 (GRCm39)	W130R	probably damaging	Het
Ubr5	G	A	15: 37,986,213 (GRCm39)	T2207I		Het
Vmn1r43	G	A	6: 89,846,877 (GRCm39)	T203M	probably damaging	Het
Vmn2r11	T	G	5: 109,201,563 (GRCm39)	I314L	possibly damaging	Het
Vmn2r15	T	A	5: 109,440,791 (GRCm39)	I356L	probably benign	Het
Vmn2r31	G	T	7: 7,397,292 (GRCm39)	T322N	probably benign	Het
Vmn2r8	A	T	5: 108,947,196 (GRCm39)	F519I	possibly damaging	Het
Wdr35	T	C	12: 9,036,092 (GRCm39)	F288L	probably benign	Het
Xab2	A	T	8: 3,663,018 (GRCm39)	V521D	probably damaging	Het
Zfp639	T	C	3: 32,574,269 (GRCm39)	F298S	probably damaging	Het
Zfp786	G	A	6: 47,797,406 (GRCm39)	R511*	probably null	Het
Zfp936	T	A	7: 42,839,834 (GRCm39)	C434S	probably damaging	Het
Zranb1	T	C	7: 132,552,146 (GRCm39)	F266L	probably benign	Het

Other mutations in Or6c65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Or6c65	APN	10	129,603,455 (GRCm39)	missense	probably null	0.99
IGL01313:Or6c65	APN	10	129,603,464 (GRCm39)	missense	probably damaging	1.00
IGL01923:Or6c65	APN	10	129,603,973 (GRCm39)	missense	probably benign	0.01
IGL02010:Or6c65	APN	10	129,604,136 (GRCm39)	missense	probably benign	0.07
IGL02393:Or6c65	APN	10	129,603,662 (GRCm39)	missense	probably benign	0.06
IGL03026:Or6c65	APN	10	129,603,910 (GRCm39)	missense	probably benign	0.00
R1181:Or6c65	UTSW	10	129,604,033 (GRCm39)	missense	probably benign	0.09
R1760:Or6c65	UTSW	10	129,603,417 (GRCm39)	missense	probably benign	0.06
R1844:Or6c65	UTSW	10	129,603,725 (GRCm39)	missense	probably benign	0.03
R2697:Or6c65	UTSW	10	129,603,793 (GRCm39)	missense	probably benign	0.09
R3025:Or6c65	UTSW	10	129,603,542 (GRCm39)	missense	probably damaging	1.00
R3414:Or6c65	UTSW	10	129,604,301 (GRCm39)	missense	probably benign
R5824:Or6c65	UTSW	10	129,604,250 (GRCm39)	missense	probably damaging	1.00
R6260:Or6c65	UTSW	10	129,603,389 (GRCm39)	missense	probably benign	0.00
R6723:Or6c65	UTSW	10	129,604,284 (GRCm39)	missense	probably benign	0.38
R6758:Or6c65	UTSW	10	129,603,920 (GRCm39)	missense	probably damaging	0.97
R7305:Or6c65	UTSW	10	129,603,720 (GRCm39)	nonsense	probably null
R7422:Or6c65	UTSW	10	129,604,136 (GRCm39)	missense	possibly damaging	0.95
R8393:Or6c65	UTSW	10	129,604,304 (GRCm39)	makesense	probably null
R8444:Or6c65	UTSW	10	129,603,794 (GRCm39)	missense	probably damaging	1.00
R8702:Or6c65	UTSW	10	129,604,284 (GRCm39)	missense	probably benign	0.38
R8717:Or6c65	UTSW	10	129,604,127 (GRCm39)	missense	probably damaging	1.00
R8797:Or6c65	UTSW	10	129,603,674 (GRCm39)	missense	probably damaging	1.00
R9356:Or6c65	UTSW	10	129,604,035 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TAATTGCCTCTTGGTCAGCTG -3'
(R):5'- TTGAGCATGGCAACTCCTTTAC -3'

Sequencing Primer
(F):5'- GCCTCTTGGTCAGCTGGATTC -3'
(R):5'- GAGCATGGCAACTCCTTTACTTAGC -3'

Posted On

2022-08-09