Mutagenetix > Incidental Mutation

Incidental Mutation 'R9603:Or4f14'

723769

Institutional Source

Beutler Lab

Gene Symbol

Or4f14

Ensembl Gene

ENSMUSG00000096566

Gene Name

olfactory receptor family 4 subfamily F member 14

Synonyms

Olfr1306, GA_x6K02T2Q125-72954873-72953935, MOR245-15

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R9603 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111742335-111743273 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 111743128 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 49 (V49A)

Ref Sequence

ENSEMBL: ENSMUSP00000151142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099607] [ENSMUST00000214844]

AlphaFold

A2BFL7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000099607
AA Change: V49A

PolyPhen 2 Score 0.686 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000097202
Gene: ENSMUSG00000096566
AA Change: V49A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	305	4.7e-43	PFAM
Pfam:7tm_1	41	287	9.7e-25	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000214844
AA Change: V49A

PolyPhen 2 Score 0.686 (Sensitivity: 0.86; Specificity: 0.92)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsl6	A	T	11: 54,225,911 (GRCm39)	N317I	probably damaging	Het
Adam18	T	A	8: 25,118,147 (GRCm39)	S590C	possibly damaging	Het
Btrc	G	A	19: 45,459,526 (GRCm39)	E103K	probably benign	Het
Cars1	A	G	7: 143,112,929 (GRCm39)	M766T	possibly damaging	Het
Cavin4	T	C	4: 48,671,999 (GRCm39)	V148A	probably benign	Het
Cdcp3	T	C	7: 130,830,643 (GRCm39)	V359A	probably damaging	Het
Cdkn2d	T	C	9: 21,202,139 (GRCm39)	D36G	possibly damaging	Het
Cubn	A	T	2: 13,292,510 (GRCm39)	D3224E	probably damaging	Het
Ebf1	A	G	11: 44,509,006 (GRCm39)	M1V	probably null	Het
Eif1ad7	T	G	12: 88,238,727 (GRCm39)	N11T	unknown	Het
Fcgbp	A	G	7: 27,802,563 (GRCm39)	D1497G	probably damaging	Het
Foxs1	A	C	2: 152,774,281 (GRCm39)	C257W	probably damaging	Het
Fstl5	C	T	3: 76,496,260 (GRCm39)	P341L	probably damaging	Het
Fzd10	G	A	5: 128,678,771 (GRCm39)	G164S	probably benign	Het
Hrh3	C	A	2: 179,742,444 (GRCm39)	E395*	probably null	Het
Hsf4	G	A	8: 105,999,435 (GRCm39)	V318M	probably damaging	Het
Il21	T	C	3: 37,281,949 (GRCm39)	E65G	possibly damaging	Het
Kank1	A	G	19: 25,408,289 (GRCm39)	D1256G	possibly damaging	Het
Klhl22	A	G	16: 17,594,915 (GRCm39)	N348S	possibly damaging	Het
Krr1	T	C	10: 111,812,672 (GRCm39)	I94T	probably damaging	Het
Lpin2	A	G	17: 71,550,410 (GRCm39)	D724G	probably damaging	Het
Mn1	C	A	5: 111,566,393 (GRCm39)	P121Q	probably damaging	Het
Mphosph9	A	T	5: 124,463,015 (GRCm39)	L10*	probably null	Het
Mtus1	A	T	8: 41,536,795 (GRCm39)	V307E	probably benign	Het
Muc20	A	G	16: 32,615,155 (GRCm39)	L74P	probably damaging	Het
Nnat	A	G	2: 157,403,701 (GRCm39)	*113W	probably null	Het
Or13a25	G	T	7: 140,247,794 (GRCm39)	C191F	probably damaging	Het
Or2f1b	C	T	6: 42,739,672 (GRCm39)	Q229*	probably null	Het
Or6b6	A	T	7: 106,571,103 (GRCm39)	Y149*	probably null	Het
Pkm	T	A	9: 59,577,831 (GRCm39)	V216E	probably damaging	Het
Pla2g12a	G	A	3: 129,674,900 (GRCm39)	V19I	unknown	Het
Pramel27	T	C	4: 143,578,267 (GRCm39)	S176P		Het
Rai14	G	A	15: 10,595,116 (GRCm39)	Q136*	probably null	Het
Rnf150	T	A	8: 83,717,208 (GRCm39)	N238K	possibly damaging	Het
Rrp1	A	G	10: 78,240,757 (GRCm39)		probably null	Het
Scaf8	T	A	17: 3,246,070 (GRCm39)	L720M	possibly damaging	Het
Slamf1	G	T	1: 171,625,771 (GRCm39)	V316L	probably damaging	Het
Slc14a1	C	A	18: 78,152,807 (GRCm39)	A367S	probably damaging	Het
Slc25a20	T	C	9: 108,549,675 (GRCm39)	F86L	probably benign	Het
Slc4a5	T	G	6: 83,217,714 (GRCm39)	S131A	probably benign	Het
Slitrk3	A	G	3: 72,958,649 (GRCm39)	I41T	probably benign	Het
Sntg1	A	T	1: 8,748,198 (GRCm39)	L94Q	probably damaging	Het
St18	T	C	1: 6,915,811 (GRCm39)	C819R	probably damaging	Het
St6galnac3	T	A	3: 153,117,177 (GRCm39)	D182V	probably benign	Het
Tubgcp2	T	A	7: 139,584,789 (GRCm39)	T549S	probably benign	Het
Vim	A	T	2: 13,579,148 (GRCm39)		probably benign	Het
Vmn2r118	C	A	17: 55,899,837 (GRCm39)	R689I	probably damaging	Het
Zbtb41	A	T	1: 139,375,255 (GRCm39)	E905V	probably damaging	Het
Zfyve9	A	T	4: 108,499,288 (GRCm39)	D1284E	possibly damaging	Het

Other mutations in Or4f14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Or4f14	APN	2	111,742,381 (GRCm39)	missense	possibly damaging	0.95
IGL01310:Or4f14	APN	2	111,742,652 (GRCm39)	missense	probably benign	0.34
IGL01893:Or4f14	APN	2	111,742,589 (GRCm39)	missense	possibly damaging	0.65
IGL02433:Or4f14	APN	2	111,742,762 (GRCm39)	missense	probably damaging	1.00
IGL03302:Or4f14	APN	2	111,743,167 (GRCm39)	missense	possibly damaging	0.61
R0544:Or4f14	UTSW	2	111,742,905 (GRCm39)	nonsense	probably null
R0674:Or4f14	UTSW	2	111,743,018 (GRCm39)	missense	probably benign	0.41
R1118:Or4f14	UTSW	2	111,743,222 (GRCm39)	missense	probably benign	0.02
R1764:Or4f14	UTSW	2	111,742,526 (GRCm39)	missense	possibly damaging	0.93
R2915:Or4f14	UTSW	2	111,743,064 (GRCm39)	missense	probably damaging	1.00
R3976:Or4f14	UTSW	2	111,742,951 (GRCm39)	missense	possibly damaging	0.84
R4855:Or4f14	UTSW	2	111,742,444 (GRCm39)	missense	probably benign	0.41
R6475:Or4f14	UTSW	2	111,743,204 (GRCm39)	nonsense	probably null
R6513:Or4f14	UTSW	2	111,743,228 (GRCm39)	missense	possibly damaging	0.89
R6536:Or4f14	UTSW	2	111,743,119 (GRCm39)	missense	possibly damaging	0.94
R6748:Or4f14	UTSW	2	111,742,702 (GRCm39)	missense	possibly damaging	0.47
R6843:Or4f14	UTSW	2	111,743,260 (GRCm39)	missense	probably damaging	1.00
R7006:Or4f14	UTSW	2	111,742,601 (GRCm39)	missense	probably benign	0.16
R7169:Or4f14	UTSW	2	111,742,939 (GRCm39)	missense	possibly damaging	0.95
R7230:Or4f14	UTSW	2	111,742,906 (GRCm39)	missense	probably damaging	1.00
R7419:Or4f14	UTSW	2	111,742,435 (GRCm39)	missense	probably damaging	1.00
R7448:Or4f14	UTSW	2	111,742,637 (GRCm39)	missense	probably benign	0.00
R7753:Or4f14	UTSW	2	111,742,927 (GRCm39)	missense	probably benign	0.06
R7761:Or4f14	UTSW	2	111,743,222 (GRCm39)	missense	probably benign	0.02
R8330:Or4f14	UTSW	2	111,742,724 (GRCm39)	missense	probably benign	0.00
R8497:Or4f14	UTSW	2	111,742,964 (GRCm39)	missense	possibly damaging	0.82
R8942:Or4f14	UTSW	2	111,743,207 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- CAAAGGCCATGGCTATGAGC -3'
(R):5'- TCTGATTACTACTAGTGCTCAGC -3'

Sequencing Primer
(F):5'- GCTATGAGCAGCACCATCTC -3'
(R):5'- TCCTTCCTCCTTCAGGCAG -3'

Posted On

2022-09-12