Mutagenetix > Incidental Mutation

Incidental Mutation 'R9604:Tas2r125'

723828

Institutional Source

Beutler Lab

Gene Symbol

Tas2r125

Ensembl Gene

ENSMUSG00000059410

Gene Name

taste receptor, type 2, member 125

Synonyms

mGR25, mt2r59, Tas2r25, T2R26

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R9604 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

132886614-132887549 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 132887023 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 137 (I137T)

Ref Sequence

ENSEMBL: ENSMUSP00000075483 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076119]

AlphaFold

Q7M710

Predicted Effect

probably benign
Transcript: ENSMUST00000076119
AA Change: I137T

PolyPhen 2 Score 0.176 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000075483
Gene: ENSMUSG00000059410
AA Change: I137T

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	1.1e-90	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg7	A	G	16: 56,597,570 (GRCm39)	F79S	probably damaging	Het
Ankrd60	A	T	2: 173,412,987 (GRCm39)	I167N	probably benign	Het
Atp13a3	G	T	16: 30,168,506 (GRCm39)	D494E	probably damaging	Het
Cep83	A	C	10: 94,554,939 (GRCm39)	H35P	possibly damaging	Het
Cpne3	A	G	4: 19,555,477 (GRCm39)	V6A	probably benign	Het
Dnm3	G	A	1: 161,838,584 (GRCm39)	A259V	possibly damaging	Het
Eftud2	T	C	11: 102,737,056 (GRCm39)	I575V	probably benign	Het
Eif4g1	A	G	16: 20,500,255 (GRCm39)	N676S	possibly damaging	Het
Foxp1	TTGCTGCTGCTGCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGCTGCTGCTG	6: 99,052,926 (GRCm39)		probably benign	Het
Gal3st4	T	A	5: 138,264,011 (GRCm39)	K329N	probably benign	Het
Gm15446	T	C	5: 110,088,314 (GRCm39)	V9A	probably damaging	Het
Golim4	A	T	3: 75,815,435 (GRCm39)		probably null	Het
Harbi1	T	C	2: 91,542,689 (GRCm39)	V50A	probably damaging	Het
Hcn2	T	C	10: 79,564,787 (GRCm39)	C400R	probably damaging	Het
Heatr6	T	C	11: 83,668,188 (GRCm39)	M894T	probably damaging	Het
Hid1	A	T	11: 115,243,466 (GRCm39)	H527Q		Het
Ifitm1	C	T	7: 140,548,227 (GRCm39)	A20V	probably benign	Het
Klk1b11	T	A	7: 43,427,850 (GRCm39)	L213Q		Het
Lepr	A	G	4: 101,590,473 (GRCm39)	Y17C	probably benign	Het
N4bp3	A	T	11: 51,536,493 (GRCm39)	V113E	probably damaging	Het
Neb	C	T	2: 52,182,680 (GRCm39)	R1062K	probably benign	Het
Nlrp4b	T	A	7: 10,444,295 (GRCm39)	N81K	probably benign	Het
Nol6	A	G	4: 41,120,298 (GRCm39)	I473T	probably benign	Het
Nsd1	T	A	13: 55,381,807 (GRCm39)	S344T	probably benign	Het
Pals2	C	A	6: 50,173,597 (GRCm39)	A487E	probably benign	Het
Pde12	A	G	14: 26,390,008 (GRCm39)	Y234H	possibly damaging	Het
Pla2g4e	C	T	2: 120,015,680 (GRCm39)	C288Y	probably benign	Het
Plekhd1	A	G	12: 80,739,731 (GRCm39)	T27A	probably damaging	Het
Pls1	T	A	9: 95,644,057 (GRCm39)	I480F	probably damaging	Het
Prom1	A	G	5: 44,187,075 (GRCm39)	I394T	probably damaging	Het
Ros1	A	T	10: 51,994,249 (GRCm39)	C1365S	probably damaging	Het
Rpgrip1l	G	A	8: 92,031,433 (GRCm39)	T148M	possibly damaging	Het
Slc14a1	C	A	18: 78,152,807 (GRCm39)	A367S	probably damaging	Het
Tap1	A	C	17: 34,412,172 (GRCm39)	D459A	probably damaging	Het
Tent5a	C	A	9: 85,206,677 (GRCm39)	V374L	probably benign	Het
Ubr4	T	C	4: 139,159,827 (GRCm39)		probably null	Het
Vmn1r172	T	C	7: 23,359,193 (GRCm39)	I26T	possibly damaging	Het

Other mutations in Tas2r125

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03252:Tas2r125	APN	6	132,887,553 (GRCm39)	splice site	probably null
R0090:Tas2r125	UTSW	6	132,887,361 (GRCm39)	missense	probably benign	0.21
R0725:Tas2r125	UTSW	6	132,887,085 (GRCm39)	missense	probably benign	0.44
R1901:Tas2r125	UTSW	6	132,887,139 (GRCm39)	missense	probably benign	0.13
R1902:Tas2r125	UTSW	6	132,887,139 (GRCm39)	missense	probably benign	0.13
R2679:Tas2r125	UTSW	6	132,887,190 (GRCm39)	missense	probably benign	0.43
R4770:Tas2r125	UTSW	6	132,886,750 (GRCm39)	missense	probably damaging	1.00
R4784:Tas2r125	UTSW	6	132,886,866 (GRCm39)	missense	probably benign	0.16
R5795:Tas2r125	UTSW	6	132,886,621 (GRCm39)	missense	probably damaging	1.00
R7535:Tas2r125	UTSW	6	132,887,287 (GRCm39)	missense	probably damaging	1.00
R7820:Tas2r125	UTSW	6	132,886,841 (GRCm39)	missense	probably benign	0.02
R9071:Tas2r125	UTSW	6	132,887,400 (GRCm39)	missense	probably benign	0.00
Z1177:Tas2r125	UTSW	6	132,886,619 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GGATCCAGATATTAAAATGGCTGTG -3'
(R):5'- GTTTTCAGGTGTCTCCACAGG -3'

Sequencing Primer
(F):5'- GGCTGTGAAAATCATTAGAATAAGC -3'
(R):5'- GCCAACAGGGACACAGTG -3'

Posted On

2022-09-12