Mutagenetix > Incidental Mutation

Incidental Mutation 'R9615:Zfp85'

724527

Institutional Source

Beutler Lab

Gene Symbol

Zfp85

Ensembl Gene

ENSMUSG00000058331

Gene Name

zinc finger protein 85

Synonyms

Zfp85-rs1, KRAB19, Zfp71

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R9615 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

67895919-67903347 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 67897326 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 249 (Y249H)

Ref Sequence

ENSEMBL: ENSMUSP00000121202 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091520] [ENSMUST00000144183]

AlphaFold

Q7TMC9

Predicted Effect

probably benign
Transcript: ENSMUST00000091520

SMART Domains

Protein: ENSMUSP00000089105
Gene: ENSMUSG00000058331

Domain	Start	End	E-Value	Type
KRAB	5	65	5.78e-29	SMART
ZnF_C2H2	81	103	1.95e-3	SMART
ZnF_C2H2	109	131	1.22e-4	SMART
ZnF_C2H2	137	159	6.32e-3	SMART
ZnF_C2H2	165	187	2.95e-3	SMART
ZnF_C2H2	193	215	3.89e-3	SMART
ZnF_C2H2	221	243	3.89e-3	SMART
ZnF_C2H2	249	271	1.1e-2	SMART
ZnF_C2H2	277	299	2.91e-2	SMART
ZnF_C2H2	305	327	2.49e-1	SMART
ZnF_C2H2	333	355	2.09e-3	SMART
ZnF_C2H2	361	383	4.17e-3	SMART
ZnF_C2H2	389	411	2.95e-3	SMART
ZnF_C2H2	417	439	1.3e-4	SMART
ZnF_C2H2	445	467	1.04e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000144183
AA Change: Y249H

PolyPhen 2 Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000121202
Gene: ENSMUSG00000058331
AA Change: Y249H

Domain	Start	End	E-Value	Type
KRAB	45	105	5.78e-29	SMART
ZnF_C2H2	121	143	1.95e-3	SMART
ZnF_C2H2	149	171	1.22e-4	SMART
ZnF_C2H2	177	199	6.32e-3	SMART
ZnF_C2H2	205	227	2.95e-3	SMART
ZnF_C2H2	233	255	3.89e-3	SMART
ZnF_C2H2	261	283	3.89e-3	SMART
ZnF_C2H2	289	311	1.1e-2	SMART
ZnF_C2H2	317	339	2.91e-2	SMART
ZnF_C2H2	345	367	2.49e-1	SMART
ZnF_C2H2	373	395	2.09e-3	SMART
ZnF_C2H2	401	423	4.17e-3	SMART
ZnF_C2H2	429	451	2.95e-3	SMART
ZnF_C2H2	457	479	1.3e-4	SMART
ZnF_C2H2	485	507	1.04e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123K08Rik	T	C	5: 138,561,814 (GRCm39)	Q124R	probably damaging	Het
Abca16	T	A	7: 120,126,404 (GRCm39)	F1188I	probably benign	Het
Acat3	G	A	17: 13,147,502 (GRCm39)	Q172*	probably null	Het
Aicda	T	A	6: 122,538,113 (GRCm39)	C90*	probably null	Het
Arhgap39	C	A	15: 76,621,438 (GRCm39)	V388L	probably benign	Het
B020011L13Rik	A	T	1: 117,729,462 (GRCm39)	H323L	probably damaging	Het
Bsn	C	T	9: 107,984,430 (GRCm39)	R436Q		Het
C3	A	T	17: 57,518,669 (GRCm39)	L1238Q	probably damaging	Het
Cert1	C	T	13: 96,767,334 (GRCm39)	H495Y	possibly damaging	Het
Ces1g	A	G	8: 94,061,807 (GRCm39)	V33A	probably damaging	Het
Clstn1	A	G	4: 149,722,757 (GRCm39)	D475G	probably damaging	Het
Cmtm2a	A	G	8: 105,019,286 (GRCm39)	V101A	probably damaging	Het
Cnnm4	A	G	1: 36,511,893 (GRCm39)	M374V	probably damaging	Het
Commd9	T	C	2: 101,727,436 (GRCm39)	S77P	possibly damaging	Het
Cox7a2l	A	G	17: 83,821,701 (GRCm39)	Y2H	possibly damaging	Het
Cramp1	C	A	17: 25,201,783 (GRCm39)	K566N	probably damaging	Het
Cubn	G	A	2: 13,325,991 (GRCm39)	H2725Y	possibly damaging	Het
Cyp2c39	T	C	19: 39,501,617 (GRCm39)	F57L	probably benign	Het
Dgkh	A	T	14: 78,813,370 (GRCm39)	V1036D	possibly damaging	Het
Dip2c	G	A	13: 9,625,191 (GRCm39)	V562I	probably benign	Het
Fat3	T	C	9: 16,289,343 (GRCm39)	Q60R	probably benign	Het
Gm1527	A	G	3: 28,969,475 (GRCm39)	D275G	probably damaging	Het
Gp5	A	C	16: 30,128,393 (GRCm39)	F94V	probably damaging	Het
Grik4	T	A	9: 42,502,765 (GRCm39)	K453*	probably null	Het
Hdlbp	T	C	1: 93,358,014 (GRCm39)	T296A	probably benign	Het
Hrh4	C	A	18: 13,154,944 (GRCm39)	T161K	probably benign	Het
Ift74	A	T	4: 94,550,822 (GRCm39)		probably null	Het
Kctd5	A	T	17: 24,292,192 (GRCm39)	Y71N	probably benign	Het
Klhl42	T	C	6: 147,009,373 (GRCm39)	L404P	probably damaging	Het
Klra5	C	A	6: 129,883,686 (GRCm39)	W147L	possibly damaging	Het
Lipe	T	A	7: 25,097,326 (GRCm39)	K206*	probably null	Het
Lipo3	T	C	19: 33,754,047 (GRCm39)	I363V	probably benign	Het
Mrgpra6	T	A	7: 46,835,675 (GRCm39)	I249F	probably benign	Het
Myo15a	A	T	11: 60,374,320 (GRCm39)	N125I		Het
Nbr1	T	A	11: 101,465,978 (GRCm39)	I753N	probably benign	Het
Ndor1	T	C	2: 25,138,434 (GRCm39)	D451G	probably benign	Het
Nlrp5	C	T	7: 23,107,561 (GRCm39)	T78I	probably benign	Het
Or10j3b	A	T	1: 173,044,034 (GRCm39)	E272V	possibly damaging	Het
Or51ag1	C	T	7: 103,155,727 (GRCm39)	R142H	probably benign	Het
Prom1	A	G	5: 44,164,399 (GRCm39)	F728S	probably damaging	Het
Ptgs2	T	A	1: 149,980,802 (GRCm39)	F456Y	probably damaging	Het
Rtl1	G	A	12: 109,556,835 (GRCm39)	A1668V	possibly damaging	Het
Runx2	A	T	17: 44,969,560 (GRCm39)	D310E	probably benign	Het
Samd4b	A	T	7: 28,106,714 (GRCm39)	I349K	probably damaging	Het
Scn10a	A	G	9: 119,487,504 (GRCm39)	M610T	possibly damaging	Het
Sec61b	A	G	4: 47,483,056 (GRCm39)	I105V	probably benign	Het
Semp2l2b	T	C	10: 21,943,611 (GRCm39)	E123G	probably benign	Het
Slc35f4	A	G	14: 49,556,306 (GRCm39)	V149A	probably benign	Het
Smndc1	T	A	19: 53,368,951 (GRCm39)	M221L	probably damaging	Het
Ssh2	A	G	11: 77,316,203 (GRCm39)	N275D	possibly damaging	Het
Tas2r124	C	T	6: 132,732,492 (GRCm39)	T267I	probably benign	Het
Thra	G	A	11: 98,651,715 (GRCm39)	R79H	probably damaging	Het
Tie1	T	C	4: 118,347,032 (GRCm39)		probably benign	Het
Umodl1	A	C	17: 31,217,152 (GRCm39)	Q1128P	possibly damaging	Het
Vps35	G	A	8: 86,010,633 (GRCm39)	R237C	probably benign	Het
Zfp78	T	A	7: 6,382,074 (GRCm39)	C343S	probably damaging	Het

Other mutations in Zfp85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0383:Zfp85	UTSW	13	67,896,791 (GRCm39)	missense	probably benign	0.05
R1187:Zfp85	UTSW	13	67,897,835 (GRCm39)	missense	probably damaging	0.99
R1510:Zfp85	UTSW	13	67,903,084 (GRCm39)	intron	probably benign
R1775:Zfp85	UTSW	13	67,897,823 (GRCm39)	missense	probably damaging	0.99
R1803:Zfp85	UTSW	13	67,899,747 (GRCm39)	missense	probably benign	0.15
R2108:Zfp85	UTSW	13	67,897,003 (GRCm39)	missense	probably benign	0.08
R2412:Zfp85	UTSW	13	67,897,765 (GRCm39)	missense	probably damaging	0.99
R3147:Zfp85	UTSW	13	67,900,612 (GRCm39)	missense	probably damaging	0.96
R4811:Zfp85	UTSW	13	67,897,745 (GRCm39)	missense	probably damaging	0.97
R5308:Zfp85	UTSW	13	67,896,974 (GRCm39)	missense	probably damaging	1.00
R5334:Zfp85	UTSW	13	67,899,803 (GRCm39)	missense	probably damaging	0.99
R5373:Zfp85	UTSW	13	67,897,577 (GRCm39)	missense	probably damaging	1.00
R7045:Zfp85	UTSW	13	67,897,712 (GRCm39)	missense	probably benign	0.00
R7391:Zfp85	UTSW	13	67,897,410 (GRCm39)	missense	probably damaging	1.00
R7438:Zfp85	UTSW	13	67,897,064 (GRCm39)	missense	probably benign	0.09
R7544:Zfp85	UTSW	13	67,897,184 (GRCm39)	missense	probably benign	0.02
R8046:Zfp85	UTSW	13	67,897,098 (GRCm39)	nonsense	probably null
R8112:Zfp85	UTSW	13	67,896,893 (GRCm39)	missense	possibly damaging	0.71
R9533:Zfp85	UTSW	13	67,897,722 (GRCm39)	missense	probably benign	0.08

Predicted Primers

PCR Primer
(F):5'- TGCTGGGTTTCATGCCAGT -3'
(R):5'- AGTGTGGGAAGGCCTTTGATA -3'

Sequencing Primer
(F):5'- CATGCCAGTATGGATTCGACG -3'
(R):5'- TGTTCACCGAAGAGTACATACTGG -3'

Posted On

2022-09-12