Mutagenetix > Incidental Mutation

Incidental Mutation 'R9618:Vmn1r228'

724683

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r228

Ensembl Gene

ENSMUSG00000060245

Gene Name

vomeronasal 1 receptor 228

Synonyms

V1re3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R9618 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20776059-20777501 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20776783 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 158 (M158V)

Ref Sequence

ENSEMBL: ENSMUSP00000072243 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072410]

AlphaFold

Q8R2A7

Predicted Effect

probably benign
Transcript: ENSMUST00000072410
AA Change: M158V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000072243
Gene: ENSMUSG00000060245
AA Change: M158V

Domain	Start	End	E-Value	Type
Pfam:TAS2R	32	317	2.6e-11	PFAM
Pfam:7tm_1	53	316	2.6e-9	PFAM
Pfam:V1R	63	321	1.3e-26	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032F04Rik	C	T	3: 68,870,069	P121L	probably damaging	Het
2310002L09Rik	C	A	4: 73,950,697	M1I	probably null	Het
2510039O18Rik	T	A	4: 147,945,416	Y614*	probably null	Het
4930562C15Rik	A	T	16: 4,849,554	S270C	unknown	Het
Ak9	A	C	10: 41,327,631	T173P		Het
BC027072	T	A	17: 71,750,822	Y620F	probably damaging	Het
C530008M17Rik	A	G	5: 76,856,770	D326G	unknown	Het
Cfc1	T	C	1: 34,536,479	L78P	probably benign	Het
Chrd	T	G	16: 20,733,628	C82W	probably damaging	Het
Cln6	T	C	9: 62,850,829	V290A	probably damaging	Het
Cps1	A	G	1: 67,157,816	D342G	possibly damaging	Het
Cryzl2	G	A	1: 157,462,008	R72H	probably benign	Het
Cyp2j6	A	T	4: 96,525,848	H393Q	probably benign	Het
Eefsec	T	C	6: 88,297,699	T428A	probably benign	Het
Ergic1	G	A	17: 26,608,645	G25S	probably damaging	Het
Esf1	T	C	2: 140,159,794	I378V	probably benign	Het
Fndc1	T	A	17: 7,771,481	T1128S	unknown	Het
Gabrp	A	T	11: 33,554,342	Y324*	probably null	Het
Gm10093	T	C	17: 78,491,685	I35T	probably damaging	Het
Gm9573	GGGGTGGGCATAGATCCTGAGGCAGAGCTGGATGCAGTGGTGGTCAGGGTGGG	GGGGTGGG	17: 35,622,043		probably benign	Het
Hadha	G	A	5: 30,134,167	T270M	possibly damaging	Het
Ino80d	G	A	1: 63,062,183	T428M	probably damaging	Het
Itgae	T	C	11: 73,120,345	F647S	possibly damaging	Het
Kank4	T	C	4: 98,765,495	R899G	possibly damaging	Het
Kcna4	T	A	2: 107,296,029	N369K	probably benign	Het
Kcnip3	G	T	2: 127,510,892	P69Q	probably benign	Het
Mcf2l	A	G	8: 12,984,320		probably benign	Het
Mtmr2	T	C	9: 13,796,019	V289A	probably benign	Het
Nxph1	T	G	6: 9,247,108	N26K	probably benign	Het
Obox1	A	G	7: 15,555,699	I138V	possibly damaging	Het
Olfr1260	A	T	2: 89,977,999	T74S	probably benign	Het
Olfr734	A	T	14: 50,320,303	Y177*	probably null	Het
Olfr872	G	A	9: 20,260,343	V168M	possibly damaging	Het
P2ry14	T	A	3: 59,115,830	M70L	probably damaging	Het
Pcnt	A	G	10: 76,352,960	Y2869H	probably damaging	Het
Pkd1l1	T	C	11: 8,961,420	R286G		Het
Pygb	C	T	2: 150,815,088	R320W		Het
Rsf1	G	GACGGCGGCC	7: 97,579,909		probably benign	Het
Slc15a5	A	T	6: 138,055,781	S192T	possibly damaging	Het
Tex15	A	G	8: 33,572,369	H609R	probably benign	Het
Tmem256	T	A	11: 69,839,384	S73T	possibly damaging	Het
Tpr	G	T	1: 150,446,228	A2338S	possibly damaging	Het
Trub2	A	T	2: 29,783,334	M119K	probably benign	Het
Vmn2r100	A	T	17: 19,522,321	H319L	probably damaging	Het
Vmn2r3	T	A	3: 64,271,303	I514F	probably damaging	Het
Wnt16	T	A	6: 22,297,892	Y252*	probably null	Het
Wnt16	G	T	6: 22,297,893	E253*	probably null	Het
Xkr4	A	T	1: 3,670,978	V124E	probably damaging	Het
Zc3h13	G	T	14: 75,330,102	R945L		Het
Zfp445	T	C	9: 122,856,723	N251D	probably damaging	Het
Zfp683	A	T	4: 134,055,654	D143V	probably benign	Het

Other mutations in Vmn1r228

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01368:Vmn1r228	APN	17	20776512	missense	probably benign	0.07
IGL03275:Vmn1r228	APN	17	20776842	missense	probably damaging	1.00
PIT4498001:Vmn1r228	UTSW	17	20776510	missense	probably benign	0.00
R0097:Vmn1r228	UTSW	17	20776363	missense	probably benign	0.05
R0097:Vmn1r228	UTSW	17	20776363	missense	probably benign	0.05
R0270:Vmn1r228	UTSW	17	20776596	missense	possibly damaging	0.60
R0279:Vmn1r228	UTSW	17	20776375	missense	probably benign	0.02
R1544:Vmn1r228	UTSW	17	20777023	missense	probably benign	0.00
R1695:Vmn1r228	UTSW	17	20776298	missense	possibly damaging	0.49
R2086:Vmn1r228	UTSW	17	20777193	missense	possibly damaging	0.71
R2275:Vmn1r228	UTSW	17	20776545	missense	probably damaging	1.00
R2965:Vmn1r228	UTSW	17	20776347	missense	probably damaging	0.99
R4425:Vmn1r228	UTSW	17	20776599	missense	probably damaging	1.00
R4447:Vmn1r228	UTSW	17	20777107	missense	probably damaging	0.96
R5031:Vmn1r228	UTSW	17	20776681	nonsense	probably null
R6345:Vmn1r228	UTSW	17	20776882	missense	probably damaging	1.00
R7064:Vmn1r228	UTSW	17	20777023	missense	probably benign	0.00
R7880:Vmn1r228	UTSW	17	20776410	missense	probably damaging	1.00
R8000:Vmn1r228	UTSW	17	20776965	missense	possibly damaging	0.88
R8290:Vmn1r228	UTSW	17	20776462	missense	probably benign	0.09
R9022:Vmn1r228	UTSW	17	20776516	missense	probably damaging	1.00
R9027:Vmn1r228	UTSW	17	20777160	missense	probably benign	0.01
R9291:Vmn1r228	UTSW	17	20776761	missense	probably benign
R9492:Vmn1r228	UTSW	17	20776600	missense	probably damaging	1.00
X0018:Vmn1r228	UTSW	17	20776701	missense	probably benign	0.08

Predicted Primers

PCR Primer
(F):5'- TCATGAAGGTGCTGGAAACTAC -3'
(R):5'- GGAGCTTTTGGGTCAAAGCAG -3'

Sequencing Primer
(F):5'- TAAGACTTCCGGAAACACGC -3'
(R):5'- AGCTTTTGGGTCAAAGCAGTTATTC -3'

Posted On

2022-09-12