Mutagenetix > Incidental Mutation

Incidental Mutation 'R9628:Wif1'

725293

Institutional Source

Beutler Lab

Gene Symbol

Wif1

Ensembl Gene

ENSMUSG00000020218

Gene Name

Wnt inhibitory factor 1

Synonyms

WIF-1

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9628 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

120869909-120936547 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 120932549 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 309 (V309A)

Ref Sequence

ENSEMBL: ENSMUSP00000020439 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020439] [ENSMUST00000175867]

AlphaFold

Q9WUA1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000020439
AA Change: V309A

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000020439
Gene: ENSMUSG00000020218
AA Change: V309A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
WIF	35	179	8.47e-90	SMART
EGF	181	210	3.88e-3	SMART
EGF	213	242	4.46e-3	SMART
EGF	245	274	4.7e-2	SMART
EGF	277	306	1.69e-3	SMART
EGF	309	338	7.95e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000175867
AA Change: V295A

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000135486
Gene: ENSMUSG00000020218
AA Change: V295A

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
WIF	35	179	8.47e-90	SMART
EGF	181	210	3.88e-3	SMART
EGF	213	242	4.46e-3	SMART
EGF	245	274	4.7e-2	SMART
EGF	295	324	7.95e0	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
PHENOTYPE: Homozygous null mice are viable and fertile but display increased susceptibility to spontaneous and induced osteosarcomas. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg7	T	C	16: 56,553,193 (GRCm39)	N635D	probably damaging	Het
Ankrd13c	G	C	3: 157,653,313 (GRCm39)	K50N	probably benign	Het
Arhgef38	T	C	3: 132,838,025 (GRCm39)	Y635C	unknown	Het
Avl9	T	A	6: 56,713,460 (GRCm39)	Y239*	probably null	Het
C2cd3	T	A	7: 100,097,961 (GRCm39)	H1449Q		Het
Ccdc141	G	A	2: 76,844,838 (GRCm39)	P1410S	probably damaging	Het
Ccz1	G	A	5: 143,925,043 (GRCm39)	T471M	possibly damaging	Het
Cdh26	T	C	2: 178,083,213 (GRCm39)	S27P		Het
Cdk11b	T	G	4: 155,734,154 (GRCm39)	D756E	unknown	Het
Celsr3	C	A	9: 108,703,559 (GRCm39)	S14*	probably null	Het
Cntrob	T	C	11: 69,213,782 (GRCm39)	T3A	possibly damaging	Het
Cracd	A	G	5: 77,004,923 (GRCm39)	E428G	unknown	Het
Csde1	T	C	3: 102,962,825 (GRCm39)	V738A	probably benign	Het
Dazap1	T	C	10: 80,118,999 (GRCm39)	Y230H	unknown	Het
Dip2a	A	G	10: 76,142,993 (GRCm39)	I264T	probably damaging	Het
Dleu7	C	A	14: 62,530,144 (GRCm39)	A86S	possibly damaging	Het
Dnmbp	T	A	19: 43,858,646 (GRCm39)	D132V	probably damaging	Het
Dppa4	T	C	16: 48,111,672 (GRCm39)	V219A	probably benign	Het
Dsg1c	T	A	18: 20,397,373 (GRCm39)	I27N	probably damaging	Het
Egr4	T	C	6: 85,489,292 (GRCm39)	Y256C	probably benign	Het
Ess2	A	T	16: 17,720,757 (GRCm39)	M363K	probably damaging	Het
Fam124a	A	G	14: 62,825,010 (GRCm39)	E168G	probably damaging	Het
Gata4	T	C	14: 63,478,545 (GRCm39)	Y18C	probably damaging	Het
Gckr	C	A	5: 31,457,934 (GRCm39)	A147D	probably damaging	Het
H1f8	G	A	6: 115,924,700 (GRCm39)	V69I	probably damaging	Het
Hook1	T	C	4: 95,901,560 (GRCm39)	L506P	probably damaging	Het
Hormad2	G	A	11: 4,377,372 (GRCm39)	P22L	probably damaging	Het
Larp1b	T	C	3: 40,916,103 (GRCm39)		probably null	Het
Ldhb	T	C	6: 142,439,862 (GRCm39)	E226G	probably damaging	Het
Lpar5	T	G	6: 125,058,948 (GRCm39)	V223G	probably damaging	Het
Lrrc37a	A	G	11: 103,394,330 (GRCm39)	V365A	probably benign	Het
Lrrtm3	T	C	10: 63,923,776 (GRCm39)	K464E	probably damaging	Het
Ly75	A	G	2: 60,158,285 (GRCm39)	I1000T	probably damaging	Het
Myo1d	C	A	11: 80,448,296 (GRCm39)	G943V	possibly damaging	Het
Nicn1	C	T	9: 108,171,708 (GRCm39)	R163C	possibly damaging	Het
Nphp4	T	C	4: 152,568,966 (GRCm39)	L35P	probably damaging	Het
Nptx2	G	A	5: 144,490,261 (GRCm39)	R230H	probably benign	Het
Ntsr1	G	A	2: 180,183,274 (GRCm39)	R328H	probably damaging	Het
Or1e25	G	A	11: 73,493,864 (GRCm39)	V153I	probably benign	Het
Or4c111	A	G	2: 88,843,670 (GRCm39)	V246A	probably damaging	Het
Or8b1	T	G	9: 38,399,871 (GRCm39)	L182R	probably benign	Het
Padi6	G	A	4: 140,464,626 (GRCm39)	T201I	probably damaging	Het
Plscr4	C	T	9: 92,354,985 (GRCm39)	T13I	possibly damaging	Het
Ppp6r1	G	A	7: 4,636,112 (GRCm39)	A782V	probably benign	Het
Rab18	G	A	18: 6,788,647 (GRCm39)	V205M	probably benign	Het
Rhobtb1	T	C	10: 69,106,653 (GRCm39)	V468A	probably damaging	Het
Rhox8	GCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	GCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	X: 36,966,991 (GRCm39)		probably benign	Het
Scaf11	A	T	15: 96,317,398 (GRCm39)	I722N	probably benign	Het
Slc17a4	A	G	13: 24,089,512 (GRCm39)	V135A	possibly damaging	Het
Slc30a5	A	G	13: 100,961,422 (GRCm39)		probably null	Het
Slc5a6	T	C	5: 31,197,746 (GRCm39)	Y321C	probably benign	Het
Spag7	A	T	11: 70,555,186 (GRCm39)	D178E	probably benign	Het
Swsap1	T	G	9: 21,867,172 (GRCm39)	S39A		Het
Tank	A	G	2: 61,483,876 (GRCm39)	T441A	probably benign	Het
Tor1aip1	C	A	1: 155,893,320 (GRCm39)	G276*	probably null	Het
Usp47	G	A	7: 111,705,999 (GRCm39)	M1220I	probably benign	Het
Vmn1r43	G	A	6: 89,846,877 (GRCm39)	T203M	probably damaging	Het
Vmn2r35	A	G	7: 7,815,702 (GRCm39)	V489A	probably benign	Het
Xpo4	T	C	14: 57,842,630 (GRCm39)	N434D	probably damaging	Het
Zfp462	A	T	4: 55,009,423 (GRCm39)	H463L	probably benign	Het

Other mutations in Wif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01384:Wif1	APN	10	120,920,855 (GRCm39)	missense	possibly damaging	0.55
IGL01707:Wif1	APN	10	120,919,890 (GRCm39)	critical splice donor site	probably null
IGL01932:Wif1	APN	10	120,931,940 (GRCm39)	missense	probably damaging	1.00
IGL02183:Wif1	APN	10	120,911,181 (GRCm39)	missense	probably damaging	1.00
R0653:Wif1	UTSW	10	120,935,704 (GRCm39)	missense	probably benign	0.01
R1858:Wif1	UTSW	10	120,919,788 (GRCm39)	splice site	probably null
R1869:Wif1	UTSW	10	120,920,824 (GRCm39)	missense	probably benign	0.00
R1871:Wif1	UTSW	10	120,920,824 (GRCm39)	missense	probably benign	0.00
R4056:Wif1	UTSW	10	120,918,099 (GRCm39)	missense	probably benign	0.04
R4057:Wif1	UTSW	10	120,918,099 (GRCm39)	missense	probably benign	0.04
R5056:Wif1	UTSW	10	120,935,684 (GRCm39)	missense	probably benign	0.00
R6242:Wif1	UTSW	10	120,870,366 (GRCm39)	missense	possibly damaging	0.82
R6504:Wif1	UTSW	10	120,870,996 (GRCm39)	missense	probably damaging	0.96
R7220:Wif1	UTSW	10	120,926,019 (GRCm39)	missense	possibly damaging	0.76
R7365:Wif1	UTSW	10	120,919,814 (GRCm39)	missense	possibly damaging	0.94
R7456:Wif1	UTSW	10	120,932,554 (GRCm39)	missense	probably benign
R7707:Wif1	UTSW	10	120,919,864 (GRCm39)	missense	probably damaging	0.97
R8283:Wif1	UTSW	10	120,931,952 (GRCm39)	missense	probably benign
R8817:Wif1	UTSW	10	120,932,621 (GRCm39)	missense	possibly damaging	0.95
R8940:Wif1	UTSW	10	120,935,684 (GRCm39)	missense	probably benign	0.11
R8959:Wif1	UTSW	10	120,931,957 (GRCm39)	missense	probably damaging	0.99
Z1176:Wif1	UTSW	10	120,932,561 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATGCGGCAGTTTCAGAGCTC -3'
(R):5'- TGTCACTATAGCAGCAGGGC -3'

Sequencing Primer
(F):5'- ACACGGCTTACTGTAAAGATCCTTC -3'
(R):5'- CAGGGCTGAGTTACAGGTTATCTAAG -3'

Posted On

2022-09-12