Mutagenetix > Incidental Mutation

Incidental Mutation 'R9631:Dpp10'

725474

Institutional Source

Beutler Lab

Gene Symbol

Dpp10

Ensembl Gene

ENSMUSG00000036815

Gene Name

dipeptidylpeptidase 10

Synonyms

6430601K09Rik, DPRP3

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9631 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

123321471-124045559 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 123341703 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 659 (Y659C)

Ref Sequence

ENSEMBL: ENSMUSP00000108225 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000112603] [ENSMUST00000112606]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000112603
AA Change: Y648C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108222
Gene: ENSMUSG00000036815
AA Change: Y648C

Domain	Start	End	E-Value	Type
low complexity region	10	25	N/A	INTRINSIC
Pfam:DPPIV_N	83	450	4.9e-118	PFAM
Pfam:Peptidase_S9	530	734	6.4e-47	PFAM
Pfam:DLH	556	711	1.4e-6	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000112606
AA Change: Y659C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108225
Gene: ENSMUSG00000036815
AA Change: Y659C

Domain	Start	End	E-Value	Type
transmembrane domain	38	60	N/A	INTRINSIC
low complexity region	64	79	N/A	INTRINSIC
Pfam:DPPIV_N	137	504	4.4e-115	PFAM
Pfam:Peptidase_S9	584	788	8.6e-48	PFAM
Pfam:DLH	604	774	1.1e-7	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001C02Rik	A	G	5: 30,482,185	D152G		Het
Atl3	A	G	19: 7,532,188	N427S	probably benign	Het
Bcar3	T	A	3: 122,508,152	D165E	possibly damaging	Het
Brap	T	C	5: 121,684,372	I437T	probably benign	Het
Bspry	T	C	4: 62,482,718		probably null	Het
Camkv	G	T	9: 107,945,791	V77L	probably benign	Het
Capn15	A	T	17: 25,963,435	D566E	probably damaging	Het
Ccar2	A	G	14: 70,151,895	V76A	probably damaging	Het
Ccdc142	T	C	6: 83,107,161	C516R	probably benign	Het
Ccdc92b	T	A	11: 74,630,019	V49E		Het
Ccr7	A	G	11: 99,145,790	V102A	probably benign	Het
Cdh23	A	T	10: 60,407,389	V1069E	possibly damaging	Het
Clpb	T	G	7: 101,785,398	V467G	possibly damaging	Het
Crebzf	T	A	7: 90,443,805	L264Q	probably damaging	Het
Ctif	A	G	18: 75,471,954	F505L	probably benign	Het
Cyp2j5	A	G	4: 96,641,285	I283T	probably benign	Het
Ddx39	C	A	8: 83,721,100	H191Q	possibly damaging	Het
Dnajc9	A	T	14: 20,388,602		probably null	Het
Dock7	A	G	4: 98,966,323	L1568P	unknown	Het
Eef1akmt3	A	G	10: 127,041,292	F35L	probably benign	Het
Etl4	A	T	2: 20,661,938	N52I	probably benign	Het
F5	T	A	1: 164,186,854	C565S	probably damaging	Het
Fam170b	T	C	14: 32,835,684	S159P	probably damaging	Het
Fitm2	A	G	2: 163,469,837	I152T	probably damaging	Het
Fn1	T	C	1: 71,586,228	D2473G	probably benign	Het
Fzd2	C	A	11: 102,606,090	D453E	probably benign	Het
Grm5	T	C	7: 87,975,352	Y376H	probably damaging	Het
H2-Q6	T	A	17: 35,425,316	H91Q	probably benign	Het
Hspa4	A	T	11: 53,269,755	V498E	possibly damaging	Het
Igsf10	A	T	3: 59,330,483	L759H	probably damaging	Het
Jph1	T	C	1: 17,091,383	T352A	probably damaging	Het
Kit	C	T	5: 75,607,029	T24M	possibly damaging	Het
Krtap28-13	T	G	1: 83,061,176	C61G	unknown	Het
Lgr5	T	A	10: 115,466,608	I293F	probably damaging	Het
Lyzl4	GGACACACTGCAGAGGGAGTGGAGAGAAAGGGACCCACCAGTGCAAGACACACTGCAGAGGGAGTGGAGAGAAAGGGACCCACCAGTGCAGGACACACTGCAGAGG	GGACACACTGCAGAGGGAGTGGAGAGAAAGGGACCCACCAGTGCAGGACACACTGCAGAGG	9: 121,583,989		probably null	Het
Mccc1	C	A	3: 35,960,036	E711*	probably null	Het
Mroh2b	C	T	15: 4,917,074	T452I	probably damaging	Het
Ms4a10	G	A	19: 10,967,087	T111I	probably damaging	Het
Myh11	A	T	16: 14,207,577	V1443E		Het
Oc90	A	G	15: 65,897,780	L76P	probably damaging	Het
Olfr1223	T	C	2: 89,145,178		probably benign	Het
Olfr1308	A	G	2: 111,961,032	F14L	probably benign	Het
Olfr1447	A	G	19: 12,901,138	V214A	possibly damaging	Het
Olfr250	G	A	9: 38,368,418	V281M	possibly damaging	Het
Olfr682-ps1	A	T	7: 105,126,400	I290N	probably damaging	Het
Olfr958	G	A	9: 39,550,212	R220*	probably null	Het
Olfr965	A	T	9: 39,719,865	T213S	possibly damaging	Het
Olfr983	A	G	9: 40,092,927	V13A	probably damaging	Het
Pgr	C	A	9: 8,900,846	Q127K	probably benign	Het
Qrich2	GCTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTG	GCTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTGCAACACACCAGGCTGAACTGCACCTGGTTG	11: 116,457,541		probably benign	Het
Rfc1	C	T	5: 65,272,508	V852M	probably damaging	Het
Rin2	T	A	2: 145,876,517	L639H	probably damaging	Het
Rpap2	T	A	5: 107,620,426	W377R	probably damaging	Het
Senp7	T	C	16: 56,155,268	S446P	probably benign	Het
Sipa1l1	A	G	12: 82,341,002	M1V	probably null	Het
Slc25a36	A	C	9: 97,100,100	S61A	probably benign	Het
Sptbn2	C	T	19: 4,738,190	R1066W	probably damaging	Het
Tgm6	A	G	2: 130,135,890	S50G	probably benign	Het
Thada	T	C	17: 84,231,156	S1475G	probably benign	Het
Tln1	G	A	4: 43,545,694	A928V	probably damaging	Het
Trip11	G	A	12: 101,893,548	L357F	probably benign	Het
Ttc3	G	T	16: 94,370,722		probably benign	Het
Ttc38	T	C	15: 85,844,458	S204P	possibly damaging	Het
Vmn1r237	A	T	17: 21,314,398	I128F	probably benign	Het
Vmn2r50	A	T	7: 10,053,063	L39*	probably null	Het
Vps4b	A	G	1: 106,780,105	V216A	possibly damaging	Het
Zfp217	T	C	2: 170,114,870	N736S	possibly damaging	Het
Zfp985	A	G	4: 147,581,285	E25G	probably damaging	Het
Zfr	C	T	15: 12,154,542	R604*	probably null	Het
Zscan18	A	G	7: 12,771,730	S577P	possibly damaging	Het

Other mutations in Dpp10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01592:Dpp10	APN	1	123334370	missense	probably damaging	1.00
IGL01618:Dpp10	APN	1	123367867	missense	probably benign
IGL02101:Dpp10	APN	1	123411826	missense	probably damaging	1.00
IGL02284:Dpp10	APN	1	124045366	splice site	probably benign
IGL02324:Dpp10	APN	1	123367802	missense	probably benign	0.02
IGL02391:Dpp10	APN	1	123650358	missense	probably damaging	0.98
IGL02458:Dpp10	APN	1	123341689	missense	probably benign	0.01
IGL02469:Dpp10	APN	1	123411803	missense	probably benign	0.01
IGL02501:Dpp10	APN	1	123686270	missense	possibly damaging	0.93
IGL02522:Dpp10	APN	1	123423652	missense	probably benign	0.24
IGL02672:Dpp10	APN	1	123376647	missense	probably benign	0.45
IGL03034:Dpp10	APN	1	123341619	missense	probably damaging	1.00
PIT1430001:Dpp10	UTSW	1	123341182	splice site	probably benign
R0104:Dpp10	UTSW	1	123367843	missense	probably benign	0.00
R0114:Dpp10	UTSW	1	123486092	missense	probably benign	0.07
R0242:Dpp10	UTSW	1	123398546	missense	possibly damaging	0.56
R0242:Dpp10	UTSW	1	123398546	missense	possibly damaging	0.56
R0682:Dpp10	UTSW	1	123905125	missense	probably damaging	0.98
R0815:Dpp10	UTSW	1	123432929	critical splice donor site	probably null
R1549:Dpp10	UTSW	1	123341380	critical splice acceptor site	probably null
R1742:Dpp10	UTSW	1	123445206	missense	probably damaging	1.00
R1859:Dpp10	UTSW	1	123353604	missense	possibly damaging	0.47
R1991:Dpp10	UTSW	1	123905106	missense	probably null	1.00
R1992:Dpp10	UTSW	1	123905106	missense	probably null	1.00
R2079:Dpp10	UTSW	1	123432992	missense	probably damaging	1.00
R2882:Dpp10	UTSW	1	123445203	missense	probably damaging	1.00
R2974:Dpp10	UTSW	1	123411705	splice site	probably benign
R3827:Dpp10	UTSW	1	123411790	missense	possibly damaging	0.56
R3852:Dpp10	UTSW	1	123485924	nonsense	probably null
R3876:Dpp10	UTSW	1	123353487	missense	probably damaging	0.98
R3899:Dpp10	UTSW	1	123353557	missense	probably damaging	1.00
R4735:Dpp10	UTSW	1	123398627	missense	probably benign	0.15
R4922:Dpp10	UTSW	1	123378153	missense	probably benign	0.44
R5457:Dpp10	UTSW	1	123411810	missense	possibly damaging	0.51
R5599:Dpp10	UTSW	1	123905076	missense	probably damaging	0.99
R5913:Dpp10	UTSW	1	123384289	missense	probably damaging	1.00
R5979:Dpp10	UTSW	1	123384283	critical splice donor site	probably null
R6378:Dpp10	UTSW	1	123411739	missense	probably damaging	1.00
R6429:Dpp10	UTSW	1	123367601	missense	possibly damaging	0.72
R6505:Dpp10	UTSW	1	123336851	missense	probably damaging	0.99
R6776:Dpp10	UTSW	1	123367656	nonsense	probably null
R6894:Dpp10	UTSW	1	123336864	missense	probably damaging	1.00
R6951:Dpp10	UTSW	1	123341650	missense	possibly damaging	0.93
R7182:Dpp10	UTSW	1	123341151	missense	probably benign	0.15
R7246:Dpp10	UTSW	1	123334377	missense	probably damaging	1.00
R7297:Dpp10	UTSW	1	123353428	nonsense	probably null
R7375:Dpp10	UTSW	1	123367795	missense	probably benign
R7387:Dpp10	UTSW	1	123341140	missense	probably benign	0.01
R7661:Dpp10	UTSW	1	123384952	missense	probably damaging	1.00
R8065:Dpp10	UTSW	1	123352660	missense	probably benign
R8067:Dpp10	UTSW	1	123352660	missense	probably benign
R8260:Dpp10	UTSW	1	123686295	missense	probably benign
R8324:Dpp10	UTSW	1	123854172	missense	probably benign	0.02
R8373:Dpp10	UTSW	1	123854229	missense	possibly damaging	0.94
R8434:Dpp10	UTSW	1	123433010	missense	probably damaging	1.00
R9068:Dpp10	UTSW	1	123432938	missense	probably damaging	1.00
R9104:Dpp10	UTSW	1	123411755	missense	probably damaging	1.00
R9477:Dpp10	UTSW	1	123376641	missense	possibly damaging	0.46
R9492:Dpp10	UTSW	1	123353430	missense	probably damaging	1.00
R9524:Dpp10	UTSW	1	123336882	missense	probably damaging	1.00
R9576:Dpp10	UTSW	1	123341680	missense	probably damaging	1.00
R9736:Dpp10	UTSW	1	123334359	missense	possibly damaging	0.64
X0019:Dpp10	UTSW	1	123398585	missense	possibly damaging	0.88
X0020:Dpp10	UTSW	1	123398582	missense	probably benign	0.36
X0021:Dpp10	UTSW	1	123432992	missense	probably damaging	1.00
X0024:Dpp10	UTSW	1	123384286	missense	probably damaging	1.00
Z1176:Dpp10	UTSW	1	123353440	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GAGCTCAATTGAGTTTCTCTGC -3'
(R):5'- TTTGGAGCAATTCTAGGATACATGG -3'

Sequencing Primer
(F):5'- AGCTCAATTGAGTTTCTCTGCATTGG -3'
(R):5'- GAGATATTGGAACATGTGCATT -3'

Posted On

2022-09-12