Mutagenetix > Incidental Mutation

Incidental Mutation 'R9636:Ifna7'

725785

Institutional Source

Beutler Lab

Gene Symbol

Ifna7

Ensembl Gene

ENSMUSG00000100713

Gene Name

interferon alpha 7

Synonyms

Ifa7

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R9636 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

88734465-88735037 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88734733 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 90 (I90T)

Ref Sequence

ENSEMBL: ENSMUSP00000100774 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105143] [ENSMUST00000170428]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000105143
AA Change: I90T

PolyPhen 2 Score 0.905 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000100774
Gene: ENSMUSG00000100713
AA Change: I90T

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	2.32e-70	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000170428

SMART Domains

Protein: ENSMUSP00000127921
Gene: ENSMUSG00000100549

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	5.54e-68	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 29 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam30	A	G	3: 98,068,312 (GRCm39)	I48M	probably benign	Het
Arnt2	T	G	7: 83,993,042 (GRCm39)	E177A	probably benign	Het
Atad3a	T	C	4: 155,833,616 (GRCm39)	T421A	possibly damaging	Het
Cdh6	A	G	15: 13,057,655 (GRCm39)	L222P	probably benign	Het
Celf3	A	G	3: 94,394,580 (GRCm39)	H304R	possibly damaging	Het
Ces1a	T	C	8: 93,759,263 (GRCm39)	E291G	probably benign	Het
Dicer1	C	T	12: 104,688,406 (GRCm39)	W390*	probably null	Het
Fsip2	A	T	2: 82,820,563 (GRCm39)	Q5432L	possibly damaging	Het
Gm12830	T	C	4: 114,698,859 (GRCm39)	S73P		Het
Hyal5	A	G	6: 24,876,656 (GRCm39)	I176M	possibly damaging	Het
Klhl3	T	C	13: 58,198,863 (GRCm39)	T259A	probably damaging	Het
Kpna3	A	G	14: 61,624,903 (GRCm39)	S147P	probably damaging	Het
Lama4	A	G	10: 38,956,500 (GRCm39)	D1141G	possibly damaging	Het
Letm2	T	C	8: 26,083,719 (GRCm39)	I171V	probably benign	Het
Nfatc1	T	A	18: 80,706,611 (GRCm39)	Q602L	possibly damaging	Het
Or13c7	T	A	4: 43,854,898 (GRCm39)	N196K	probably damaging	Het
Or4k45	A	T	2: 111,395,786 (GRCm39)	M1K	probably null	Het
Ppp4r4	T	C	12: 103,564,688 (GRCm39)	V18A	unknown	Het
Prkdc	G	A	16: 15,548,341 (GRCm39)	R1983H	probably benign	Het
Ptprz1	A	G	6: 22,999,994 (GRCm39)	R695G	probably benign	Het
Rbbp6	AAAGAAGAAGAAGAAGAAG	AAAGAAGAAGAAGAAG	7: 122,601,175 (GRCm39)		probably benign	Het
Riok1	T	C	13: 38,242,719 (GRCm39)		probably null	Het
Rnf180	T	A	13: 105,386,819 (GRCm39)	H164L	possibly damaging	Het
Slc2a3	A	G	6: 122,709,362 (GRCm39)	S346P	probably damaging	Het
Slit3	A	T	11: 35,594,088 (GRCm39)	D1426V	probably damaging	Het
Try10	A	G	6: 41,332,505 (GRCm39)	N54D	probably benign	Het
Vmn2r71	T	C	7: 85,268,388 (GRCm39)	V197A	possibly damaging	Het
Wdfy3	C	A	5: 102,047,899 (GRCm39)	R1802L	probably benign	Het
Zfyve16	T	C	13: 92,631,456 (GRCm39)	R1380G	probably benign	Het

Other mutations in Ifna7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00597:Ifna7	APN	4	88,734,675 (GRCm39)	missense	probably benign	0.00
R1326:Ifna7	UTSW	4	88,734,931 (GRCm39)	missense	possibly damaging	0.87
R1352:Ifna7	UTSW	4	88,734,897 (GRCm39)	missense	possibly damaging	0.76
R1420:Ifna7	UTSW	4	88,734,906 (GRCm39)	missense	probably damaging	0.98
R1655:Ifna7	UTSW	4	88,734,897 (GRCm39)	missense	probably benign	0.06
R3767:Ifna7	UTSW	4	88,734,964 (GRCm39)	missense	probably damaging	1.00
R3768:Ifna7	UTSW	4	88,734,964 (GRCm39)	missense	probably damaging	1.00
R3769:Ifna7	UTSW	4	88,734,964 (GRCm39)	missense	probably damaging	1.00
R3770:Ifna7	UTSW	4	88,734,964 (GRCm39)	missense	probably damaging	1.00
R7478:Ifna7	UTSW	4	88,734,913 (GRCm39)	missense	probably damaging	1.00
R7549:Ifna7	UTSW	4	88,734,664 (GRCm39)	missense	possibly damaging	0.47

Predicted Primers

PCR Primer
(F):5'- TGTTGAGCTACTGGCCAACC -3'
(R):5'- AAGTATGTCCTCACAGCCAGC -3'

Sequencing Primer
(F):5'- GGCCAACCTGCTCTCTAGGATATG -3'
(R):5'- CAGGGCATCTTCCTGGGTCAG -3'

Posted On

2022-09-12