Mutagenetix > Incidental Mutation

Incidental Mutation 'R9645:Or1e35'

726228

Institutional Source

Beutler Lab

Gene Symbol

Or1e35

Ensembl Gene

ENSMUSG00000062186

Gene Name

olfactory receptor family 1 subfamily E member 35

Synonyms

MOR135-10, GA_x6K02T2P1NL-4062605-4061667, Olfr395

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R9645 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73797378-73798316 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 73797713 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 202 (M202L)

Ref Sequence

ENSEMBL: ENSMUSP00000149064 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072991] [ENSMUST00000215690]

AlphaFold

Q8VGR3

Predicted Effect

probably benign
Transcript: ENSMUST00000072991
AA Change: M202L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000072756
Gene: ENSMUSG00000062186
AA Change: M202L

Domain	Start	End	E-Value	Type
low complexity region	8	17	N/A	INTRINSIC
Pfam:7tm_4	31	309	6.2e-56	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.8e-8	PFAM
Pfam:7tm_1	41	290	3.7e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215690
AA Change: M202L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430033K04Rik	T	C	5: 138,644,793 (GRCm39)	V226A	probably benign	Het
Acan	G	T	7: 78,749,653 (GRCm39)	V1475F	probably benign	Het
Acin1	T	A	14: 54,901,913 (GRCm39)	R626S	probably benign	Het
Aff3	T	C	1: 38,249,121 (GRCm39)	K662R	probably damaging	Het
Ankrd11	C	T	8: 123,617,682 (GRCm39)	A2057T	probably benign	Het
Ano9	T	G	7: 140,687,388 (GRCm39)	N309T	probably benign	Het
Ap3d1	G	A	10: 80,545,062 (GRCm39)	S1092L	probably benign	Het
B4galt7	C	T	13: 55,756,556 (GRCm39)	H257Y	probably damaging	Het
BC051665	G	T	13: 60,932,545 (GRCm39)	Q47K	possibly damaging	Het
Brip1	A	G	11: 85,952,512 (GRCm39)	F1090L	probably benign	Het
Cabin1	A	G	10: 75,494,543 (GRCm39)	V1612A	probably benign	Het
Ccar1	A	T	10: 62,602,369 (GRCm39)	D434E	probably benign	Het
Ccdc15	T	C	9: 37,227,083 (GRCm39)	D297G	probably benign	Het
Celsr3	C	A	9: 108,704,691 (GRCm39)	Y391*	probably null	Het
Cfap52	A	G	11: 67,837,179 (GRCm39)	I194T	possibly damaging	Het
Ctif	T	C	18: 75,757,352 (GRCm39)	E67G	probably benign	Het
Cxcr6	T	C	9: 123,639,151 (GRCm39)	F58L	possibly damaging	Het
Cyp2c69	T	C	19: 39,869,593 (GRCm39)	E142G	probably damaging	Het
Dnah6	T	A	6: 73,115,750 (GRCm39)	R1549S	possibly damaging	Het
Duxf3	A	T	10: 58,066,803 (GRCm39)	H75Q	probably benign	Het
Ehbp1	A	T	11: 22,051,052 (GRCm39)	I500N	probably damaging	Het
F13a1	A	T	13: 37,082,154 (GRCm39)	S517T	probably benign	Het
Fbln7	C	T	2: 128,719,316 (GRCm39)	R38C	probably damaging	Het
Fn1	T	C	1: 71,667,629 (GRCm39)	N788S	probably benign	Het
Foxc1	A	T	13: 31,991,882 (GRCm39)	E231V	probably damaging	Het
Gal3st2b	T	C	1: 93,866,328 (GRCm39)	S10P	probably damaging	Het
Gm6899	G	A	11: 26,543,592 (GRCm39)	C53Y	unknown	Het
Hps3	T	C	3: 20,084,831 (GRCm39)	E119G	probably benign	Het
Impg2	A	G	16: 56,038,767 (GRCm39)	E135G	probably damaging	Het
Kalrn	G	A	16: 34,032,583 (GRCm39)	T1155M	probably benign	Het
Kcna6	G	A	6: 126,716,022 (GRCm39)	A289V	probably benign	Het
Kcnh5	T	C	12: 75,134,191 (GRCm39)	M453V	probably benign	Het
Krt222	A	G	11: 99,131,320 (GRCm39)	V75A	possibly damaging	Het
Lmtk3	A	T	7: 45,450,431 (GRCm39)	T1432S	unknown	Het
Ltbp2	G	A	12: 84,837,864 (GRCm39)	P1192L	probably benign	Het
Ltbp3	C	G	19: 5,802,099 (GRCm39)	N758K	probably damaging	Het
Map6d1	T	A	16: 20,055,377 (GRCm39)	H153L	possibly damaging	Het
Mefv	A	G	16: 3,528,782 (GRCm39)	L583P	probably damaging	Het
Mrps28	T	C	3: 8,867,389 (GRCm39)	T160A	probably damaging	Het
Mtcl2	T	C	2: 156,869,390 (GRCm39)	K1082E	probably damaging	Het
Muc6	T	C	7: 141,217,783 (GRCm39)	T2297A	possibly damaging	Het
Mx1	G	T	16: 97,253,409 (GRCm39)	D338E	probably benign	Het
Myom1	G	A	17: 71,399,204 (GRCm39)	R1120H	probably benign	Het
Nif3l1	T	C	1: 58,487,074 (GRCm39)	F87L	probably benign	Het
Or4p22	T	C	2: 88,317,373 (GRCm39)	V99A	possibly damaging	Het
Pex10	T	A	4: 155,153,264 (GRCm39)	L111Q	probably damaging	Het
Phf1	T	C	17: 27,154,130 (GRCm39)		probably null	Het
Pigp	A	T	16: 94,166,278 (GRCm39)	Y143*	probably null	Het
Pou2af2	A	G	9: 51,229,579 (GRCm39)		probably null	Het
Prrc2a	A	G	17: 35,381,176 (GRCm39)		probably null	Het
Psd	GCC	GC	19: 46,301,841 (GRCm39)		probably null	Het
Pum3	A	G	19: 27,403,412 (GRCm39)	S30P	probably benign	Het
Rab12	A	G	17: 66,826,421 (GRCm39)	S97P	probably damaging	Het
Rigi	T	C	4: 40,220,437 (GRCm39)	E482G	possibly damaging	Het
Ros1	A	G	10: 51,948,148 (GRCm39)		probably null	Het
Scp2	A	T	4: 107,948,519 (GRCm39)	Y197N	probably benign	Het
Serpinb10	T	C	1: 107,474,488 (GRCm39)	F217L	possibly damaging	Het
Shank3	T	G	15: 89,409,453 (GRCm39)	M604R	possibly damaging	Het
Slc22a29	A	G	19: 8,184,488 (GRCm39)	S238P	probably benign	Het
Smarcc1	A	G	9: 109,986,410 (GRCm39)	D237G	probably damaging	Het
Stag3	T	C	5: 138,299,701 (GRCm39)	S871P	possibly damaging	Het
Tmem132d	A	G	5: 128,346,075 (GRCm39)	F149S	probably damaging	Het
Tmem25	C	T	9: 44,706,515 (GRCm39)	E333K		Het
Togaram1	T	A	12: 65,066,082 (GRCm39)	Y1695*	probably null	Het
Tox3	G	A	8: 90,984,574 (GRCm39)	P202S	probably damaging	Het
Trim34b	T	A	7: 103,980,474 (GRCm39)	N187K	probably benign	Het
Vcan	A	G	13: 89,841,081 (GRCm39)	S1488P	probably benign	Het
Vps26a	A	C	10: 62,305,791 (GRCm39)	V124G	probably benign	Het
Vps50	T	G	6: 3,516,706 (GRCm39)	S63A	possibly damaging	Het
Wbp4	T	A	14: 79,707,553 (GRCm39)	E186V	possibly damaging	Het
Zfp52	A	G	17: 21,781,937 (GRCm39)	D595G	possibly damaging	Het
Zfr2	C	A	10: 81,084,252 (GRCm39)	S1*	probably null	Het

Other mutations in Or1e35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01288:Or1e35	APN	11	73,798,139 (GRCm39)	missense	probably damaging	1.00
IGL01618:Or1e35	APN	11	73,798,303 (GRCm39)	missense	probably damaging	1.00
IGL01995:Or1e35	APN	11	73,798,261 (GRCm39)	missense	possibly damaging	0.52
IGL02494:Or1e35	APN	11	73,797,550 (GRCm39)	missense	possibly damaging	0.55
IGL02995:Or1e35	APN	11	73,798,045 (GRCm39)	missense	possibly damaging	0.90
IGL03256:Or1e35	APN	11	73,797,522 (GRCm39)	missense	probably benign	0.07
R0018:Or1e35	UTSW	11	73,797,452 (GRCm39)	missense	probably damaging	1.00
R0701:Or1e35	UTSW	11	73,797,655 (GRCm39)	missense	probably damaging	1.00
R0839:Or1e35	UTSW	11	73,798,138 (GRCm39)	missense	probably damaging	0.99
R1222:Or1e35	UTSW	11	73,798,240 (GRCm39)	missense	probably damaging	1.00
R1737:Or1e35	UTSW	11	73,797,911 (GRCm39)	missense	possibly damaging	0.69
R1819:Or1e35	UTSW	11	73,797,505 (GRCm39)	missense	probably benign	0.02
R2994:Or1e35	UTSW	11	73,797,541 (GRCm39)	missense	probably damaging	1.00
R3195:Or1e35	UTSW	11	73,797,484 (GRCm39)	missense	possibly damaging	0.91
R4622:Or1e35	UTSW	11	73,797,737 (GRCm39)	missense	possibly damaging	0.55
R4753:Or1e35	UTSW	11	73,797,677 (GRCm39)	missense	probably damaging	1.00
R5137:Or1e35	UTSW	11	73,797,452 (GRCm39)	missense	probably damaging	1.00
R5448:Or1e35	UTSW	11	73,797,437 (GRCm39)	missense	probably damaging	1.00
R5604:Or1e35	UTSW	11	73,797,853 (GRCm39)	missense	probably benign	0.02
R5748:Or1e35	UTSW	11	73,797,721 (GRCm39)	missense	probably damaging	0.98
R5899:Or1e35	UTSW	11	73,797,755 (GRCm39)	missense	probably damaging	1.00
R6156:Or1e35	UTSW	11	73,797,447 (GRCm39)	nonsense	probably null
R6388:Or1e35	UTSW	11	73,798,118 (GRCm39)	missense	probably damaging	1.00
R6572:Or1e35	UTSW	11	73,797,629 (GRCm39)	missense	possibly damaging	0.91
R7241:Or1e35	UTSW	11	73,798,058 (GRCm39)	missense	probably benign	0.05
R8870:Or1e35	UTSW	11	73,797,725 (GRCm39)	missense	probably benign	0.03
R9358:Or1e35	UTSW	11	73,797,451 (GRCm39)	missense	probably damaging	1.00
R9544:Or1e35	UTSW	11	73,797,637 (GRCm39)	missense	probably benign	0.14
R9667:Or1e35	UTSW	11	73,798,097 (GRCm39)	missense	possibly damaging	0.83
R9707:Or1e35	UTSW	11	73,798,090 (GRCm39)	missense	possibly damaging	0.79
Z1177:Or1e35	UTSW	11	73,797,439 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACATCATGGCCATGGAAATCTC -3'
(R):5'- GTGCTACTACTGTGGATGCTGAC -3'

Sequencing Primer
(F):5'- GGCCATGGAAATCTCATTCACAGTAG -3'
(R):5'- GTGGATGCTGACAATATCCCATGC -3'

Posted On

2022-09-12