Mutagenetix > Incidental Mutation

Incidental Mutation 'R9043:Mapk10'

726262

Institutional Source

Beutler Lab

Gene Symbol

Mapk10

Ensembl Gene

ENSMUSG00000046709

Gene Name

mitogen-activated protein kinase 10

Synonyms

p493F12, C230008H04Rik, JNK3, Serk2

MMRRC Submission

068870-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9043 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

103056413-103359200 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 103074025 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000127193 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086854] [ENSMUST00000112846] [ENSMUST00000112847] [ENSMUST00000112848] [ENSMUST00000141573] [ENSMUST00000170792]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000086854

SMART Domains

Protein: ENSMUSP00000084065
Gene: ENSMUSG00000046709

Domain	Start	End	E-Value	Type
S_TKc	64	359	5.76e-88	SMART
low complexity region	423	432	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112846

SMART Domains

Protein: ENSMUSP00000108467
Gene: ENSMUSG00000046709

Domain	Start	End	E-Value	Type
S_TKc	64	359	4.37e-88	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112847

SMART Domains

Protein: ENSMUSP00000108468
Gene: ENSMUSG00000046709

Domain	Start	End	E-Value	Type
S_TKc	64	359	4.37e-88	SMART
low complexity region	423	432	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112848

SMART Domains

Protein: ENSMUSP00000108469
Gene: ENSMUSG00000046709

Domain	Start	End	E-Value	Type
S_TKc	94	389	4.37e-88	SMART
low complexity region	453	462	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000141573

SMART Domains

Protein: ENSMUSP00000142798
Gene: ENSMUSG00000046709

Domain	Start	End	E-Value	Type
Pfam:Pkinase	64	125	1.3e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000170792

SMART Domains

Protein: ENSMUSP00000127193
Gene: ENSMUSG00000046709

Domain	Start	End	E-Value	Type
S_TKc	64	359	4.37e-88	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.0%

Validation Efficiency

99% (87/88)

MGI Phenotype

FUNCTION: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals and are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for disruptions in this gene display a normal phenotype. They are resistant to kainic acid induced seizures and show increased resistance to MPTP induced Parkinson's disease. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933402J07Rik	T	A	8: 88,309,238 (GRCm39)	S134T	possibly damaging	Het
Aadacl4	T	A	4: 144,349,790 (GRCm39)	L349Q	probably damaging	Het
Ankrd29	T	C	18: 12,428,970 (GRCm39)	N33S	probably damaging	Het
Arid3b	T	C	9: 57,699,900 (GRCm39)	S536G	possibly damaging	Het
Art5	A	C	7: 101,748,699 (GRCm39)	M1R	probably null	Het
Arvcf	T	A	16: 18,218,452 (GRCm39)	C545S	probably damaging	Het
Atpsckmt	A	G	15: 31,617,101 (GRCm39)	T189A	probably benign	Het
Axin2	T	A	11: 108,833,794 (GRCm39)	N580K	probably benign	Het
Cdh23	T	G	10: 60,151,478 (GRCm39)	D2469A	probably benign	Het
Cep350	T	A	1: 155,773,228 (GRCm39)	T1781S	probably damaging	Het
Cfap65	T	A	1: 74,943,847 (GRCm39)	T1555S	possibly damaging	Het
Chga	G	A	12: 102,529,054 (GRCm39)	E344K	possibly damaging	Het
Ckap2l	A	T	2: 129,126,892 (GRCm39)	L429M	probably damaging	Het
Cpne7	C	T	8: 123,856,951 (GRCm39)	P402L	probably damaging	Het
Cyp2g1	A	G	7: 26,509,256 (GRCm39)	N110S	probably benign	Het
Cyp3a11	T	C	5: 145,805,995 (GRCm39)	D124G	probably benign	Het
Dgkq	A	T	5: 108,801,061 (GRCm39)	V527D	probably damaging	Het
Dnah3	T	C	7: 119,551,272 (GRCm39)	I37V	probably benign	Het
Dnah9	T	A	11: 65,845,680 (GRCm39)	E3068V		Het
Dusp23	T	C	1: 172,460,184 (GRCm39)	E89G	probably null	Het
Fam187a	A	G	11: 102,776,685 (GRCm39)	Y163C	possibly damaging	Het
Gabrg2	T	A	11: 41,865,662 (GRCm39)	I106F	probably damaging	Het
Gpc2	A	G	5: 138,277,193 (GRCm39)	I78T	probably benign	Het
Gpr149	A	C	3: 62,511,360 (GRCm39)	L213R	probably damaging	Het
Grm1	A	T	10: 10,565,056 (GRCm39)	L1084*	probably null	Het
Gypa	A	G	8: 81,222,946 (GRCm39)	T17A	unknown	Het
Gys2	A	T	6: 142,376,059 (GRCm39)	F534L	probably damaging	Het
Iqce	A	T	5: 140,651,810 (GRCm39)	M721K	probably benign	Het
Klrc2	T	A	6: 129,635,741 (GRCm39)	Y129F	probably damaging	Het
Krt14	T	C	11: 100,095,464 (GRCm39)	S314G	possibly damaging	Het
L3mbtl3	T	C	10: 26,156,152 (GRCm39)	Q685R	unknown	Het
Lamb3	A	T	1: 193,007,919 (GRCm39)	K206*	probably null	Het
Lgr5	A	G	10: 115,314,343 (GRCm39)	I198T	probably damaging	Het
Mcm6	C	T	1: 128,271,231 (GRCm39)	D463N	probably damaging	Het
Med15	A	T	16: 17,470,582 (GRCm39)	W742R	probably benign	Het
Meis1	T	A	11: 18,831,916 (GRCm39)	Q374L	possibly damaging	Het
Nrxn3	T	C	12: 89,227,252 (GRCm39)	C296R	probably damaging	Het
Ntaq1	A	G	15: 58,014,004 (GRCm39)	Y67C	possibly damaging	Het
Oaf	C	T	9: 43,135,308 (GRCm39)	A157T	probably damaging	Het
Oca2	T	A	7: 55,927,189 (GRCm39)		probably null	Het
Or5b110-ps1	A	T	19: 13,260,074 (GRCm39)	M116K	probably damaging	Het
Or5b12	T	A	19: 12,897,667 (GRCm39)	E2V	probably benign	Het
Or5d36	T	A	2: 87,900,983 (GRCm39)	T248S	possibly damaging	Het
Or9k2b	A	T	10: 130,016,154 (GRCm39)	H198Q	probably benign	Het
Pacs1	A	G	19: 5,188,964 (GRCm39)	I806T	probably benign	Het
Pax2	T	A	19: 44,804,499 (GRCm39)	S257T	probably benign	Het
Pbxip1	A	T	3: 89,350,794 (GRCm39)	T72S	probably benign	Het
Pcdhb14	T	C	18: 37,581,851 (GRCm39)	I319T	probably damaging	Het
Pcdhga2	C	A	18: 37,802,963 (GRCm39)	A269E	possibly damaging	Het
Pdzph1	A	C	17: 59,280,535 (GRCm39)	N582K	probably benign	Het
Plk2	A	T	13: 110,533,194 (GRCm39)	I186F	probably damaging	Het
Pzp	A	T	6: 128,471,742 (GRCm39)	C895S	probably damaging	Het
Rasa2	A	T	9: 96,484,770 (GRCm39)	V148D	probably damaging	Het
Rgsl1	A	T	1: 153,717,567 (GRCm39)	I14N	possibly damaging	Het
Rhox3c	C	T	X: 36,651,303 (GRCm39)		probably benign	Het
Rnf213	A	G	11: 119,349,739 (GRCm39)	R3867G		Het
Rpap3	A	G	15: 97,584,443 (GRCm39)	Y334H	possibly damaging	Het
Scara3	A	C	14: 66,169,070 (GRCm39)	N182K	probably damaging	Het
Sh3bp1	A	G	15: 78,791,449 (GRCm39)	E412G	possibly damaging	Het
Sh3tc2	T	A	18: 62,122,961 (GRCm39)	I574N	possibly damaging	Het
Slc16a11	T	C	11: 70,106,520 (GRCm39)	Y253H	probably damaging	Het
Slc38a4	A	T	15: 96,906,805 (GRCm39)	V311E	possibly damaging	Het
Slc4a7	T	C	14: 14,775,048 (GRCm38)	L826P	probably damaging	Het
Spag9	T	G	11: 93,951,085 (GRCm39)	V83G		Het
Sspo	T	G	6: 48,470,214 (GRCm39)	V4560G	probably benign	Het
Stxbp2	T	A	8: 3,684,478 (GRCm39)	F144I		Het
Tafa2	T	C	10: 123,540,294 (GRCm39)	V50A	probably damaging	Het
Tdh	T	C	14: 63,730,278 (GRCm39)	I334V	probably benign	Het
Tesk2	C	A	4: 116,660,148 (GRCm39)	S451Y	probably benign	Het
Tjp1	A	T	7: 64,962,679 (GRCm39)	V1166E	probably benign	Het
Tmeff2	G	A	1: 51,018,779 (GRCm39)	V254M	unknown	Het
Tmem126a	A	G	7: 90,100,749 (GRCm39)		probably null	Het
Togaram2	C	G	17: 71,993,699 (GRCm39)	P2A	probably benign	Het
Trappc14	G	A	5: 138,259,889 (GRCm39)	T389I	possibly damaging	Het
Tssk4	C	T	14: 55,889,211 (GRCm39)	R246W	probably damaging	Het
Tubal3	C	T	13: 3,982,708 (GRCm39)	R163*	probably null	Het
Usp48	G	A	4: 137,340,996 (GRCm39)	G332E	probably benign	Het
Vezt	T	C	10: 93,820,027 (GRCm39)	N446S	probably damaging	Het
Vim	A	T	2: 13,579,249 (GRCm39)	T3S	unknown	Het
Vmn2r45	A	G	7: 8,474,904 (GRCm39)	L708P	possibly damaging	Het
Yes1	A	G	5: 32,810,312 (GRCm39)	Y157C	probably damaging	Het
Zfp358	A	G	8: 3,545,810 (GRCm39)	T131A	probably benign	Het
Zfp426	C	T	9: 20,386,308 (GRCm39)	V58M	probably damaging	Het
Zfp692	T	C	11: 58,198,550 (GRCm39)	S47P	probably damaging	Het
Zfp719	A	T	7: 43,239,605 (GRCm39)	T398S	possibly damaging	Het

Other mutations in Mapk10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01480:Mapk10	APN	5	103,074,018 (GRCm39)	splice site	probably benign
IGL01791:Mapk10	APN	5	103,144,514 (GRCm39)	missense	probably damaging	1.00
IGL01885:Mapk10	APN	5	103,144,455 (GRCm39)	missense	probably damaging	1.00
IGL02192:Mapk10	APN	5	103,137,513 (GRCm39)	missense	probably damaging	0.97
IGL02260:Mapk10	APN	5	103,186,534 (GRCm39)	missense	probably benign	0.09
IGL02409:Mapk10	APN	5	103,076,096 (GRCm39)	missense	possibly damaging	0.50
IGL03148:Mapk10	APN	5	103,073,971 (GRCm39)	missense	probably damaging	1.00
R0904:Mapk10	UTSW	5	103,135,146 (GRCm39)	splice site	probably benign
R1067:Mapk10	UTSW	5	103,139,723 (GRCm39)	splice site	probably benign
R1592:Mapk10	UTSW	5	103,186,487 (GRCm39)	missense	possibly damaging	0.89
R1812:Mapk10	UTSW	5	103,061,128 (GRCm39)	missense	probably damaging	1.00
R2364:Mapk10	UTSW	5	103,186,507 (GRCm39)	missense	possibly damaging	0.81
R2866:Mapk10	UTSW	5	103,186,548 (GRCm39)	missense	probably benign	0.25
R2867:Mapk10	UTSW	5	103,186,548 (GRCm39)	missense	probably benign	0.25
R2867:Mapk10	UTSW	5	103,186,548 (GRCm39)	missense	probably benign	0.25
R4622:Mapk10	UTSW	5	103,137,590 (GRCm39)	missense	probably damaging	1.00
R4860:Mapk10	UTSW	5	103,138,485 (GRCm39)	missense	probably damaging	1.00
R4860:Mapk10	UTSW	5	103,138,485 (GRCm39)	missense	probably damaging	1.00
R4866:Mapk10	UTSW	5	103,111,391 (GRCm39)	missense	probably damaging	1.00
R5901:Mapk10	UTSW	5	103,061,158 (GRCm39)	missense	probably damaging	1.00
R5986:Mapk10	UTSW	5	103,186,446 (GRCm39)	missense	probably benign	0.33
R6000:Mapk10	UTSW	5	103,114,342 (GRCm39)	missense	probably damaging	1.00
R6000:Mapk10	UTSW	5	103,114,341 (GRCm39)	missense	probably damaging	1.00
R7375:Mapk10	UTSW	5	103,124,256 (GRCm39)	missense	probably null	0.26
R7460:Mapk10	UTSW	5	103,186,443 (GRCm39)	missense	probably benign	0.37
R7753:Mapk10	UTSW	5	103,186,419 (GRCm39)	nonsense	probably null
R7879:Mapk10	UTSW	5	103,111,362 (GRCm39)	missense	probably benign	0.10
R7935:Mapk10	UTSW	5	103,139,792 (GRCm39)	missense	possibly damaging	0.92
R8059:Mapk10	UTSW	5	103,114,478 (GRCm39)	missense	probably damaging	1.00
R8846:Mapk10	UTSW	5	103,144,521 (GRCm39)	missense	probably damaging	1.00
R9030:Mapk10	UTSW	5	103,144,499 (GRCm39)	missense	probably damaging	1.00
R9106:Mapk10	UTSW	5	103,186,442 (GRCm39)	missense	probably damaging	1.00
R9115:Mapk10	UTSW	5	103,186,532 (GRCm39)	missense
R9398:Mapk10	UTSW	5	103,061,152 (GRCm39)	missense	probably damaging	1.00
R9620:Mapk10	UTSW	5	103,114,473 (GRCm39)	missense	probably damaging	1.00
R9796:Mapk10	UTSW	5	103,135,101 (GRCm39)	missense	possibly damaging	0.93
Z1176:Mapk10	UTSW	5	103,139,753 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGGGAAACGGCCATGTGATATC -3'
(R):5'- TTCTTAACTTCAGTGCTGAGGACC -3'

Sequencing Primer
(F):5'- GAAACGGCCATGTGATATCTGTTCC -3'
(R):5'- CAGTGCTGAGGACCTGCTTTTG -3'

Posted On

2022-09-14