Mutagenetix > Incidental Mutation

Incidental Mutation 'R9649:Gm7298'

726945

Institutional Source

Beutler Lab

Gene Symbol

Gm7298

Ensembl Gene

ENSMUSG00000108022

Gene Name

predicted gene 7298

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.235) question?

Stock #

R9649 (G1)

Quality Score

145.008

Status

Not validated

Chromosome

Chromosomal Location

121711454-121761598 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 121764491 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 1457 (T1457S)

Ref Sequence

ENSEMBL: ENSMUSP00000145242 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000204124]

AlphaFold

A0A0N4SVU1

Predicted Effect

probably damaging
Transcript: ENSMUST00000204124
AA Change: T1457S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000145242
Gene: ENSMUSG00000108022
AA Change: T1457S

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:A2M_N	128	221	4e-18	PFAM
A2M_N_2	449	599	1e-45	SMART
A2M	740	830	2.1e-39	SMART
Pfam:Thiol-ester_cl	963	992	1.9e-15	PFAM
Pfam:A2M_comp	1012	1268	1.6e-90	PFAM
A2M_recep	1378	1465	4.3e-42	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010B08Rik	A	T	2: 173,561,706 (GRCm39)	H5L	unknown	Het
Aatk	A	G	11: 119,901,733 (GRCm39)	S888P	probably damaging	Het
Ahnak	A	T	19: 8,985,786 (GRCm39)	K2357*	probably null	Het
Arl9	T	A	5: 77,155,139 (GRCm39)	L90Q	probably damaging	Het
Atosa	A	G	9: 74,924,349 (GRCm39)	D864G	possibly damaging	Het
Atxn2	T	C	5: 121,949,055 (GRCm39)	V1144A	probably damaging	Het
Batf3	C	T	1: 190,830,623 (GRCm39)		probably benign	Het
Bltp3b	C	A	10: 89,626,593 (GRCm39)	T429K	probably benign	Het
Brpf3	G	A	17: 29,037,597 (GRCm39)	E822K	probably benign	Het
Cabin1	T	A	10: 75,575,239 (GRCm39)	Q602L	probably damaging	Het
Cadps	T	C	14: 12,597,418 (GRCm38)	E424G	probably damaging	Het
Camta1	A	G	4: 151,216,004 (GRCm39)	L972P	possibly damaging	Het
Ccdc159	A	G	9: 21,840,672 (GRCm39)	N80D	possibly damaging	Het
Cdh1	A	C	8: 107,388,604 (GRCm39)	E553D	possibly damaging	Het
Cdk14	T	A	5: 5,423,477 (GRCm39)	E35V	probably benign	Het
Cfap70	A	G	14: 20,450,546 (GRCm39)	L995P	probably damaging	Het
Col18a1	T	A	10: 76,916,673 (GRCm39)	E334V	unknown	Het
Colec12	T	A	18: 9,877,000 (GRCm39)	I741K	unknown	Het
Crot	A	G	5: 9,024,170 (GRCm39)	V342A	probably benign	Het
Ctnnal1	A	G	4: 56,865,036 (GRCm39)	S27P	possibly damaging	Het
Cyp2j9	A	G	4: 96,460,193 (GRCm39)	S437P	probably damaging	Het
Dennd4c	A	T	4: 86,743,160 (GRCm39)	T1001S	probably benign	Het
Dffa	G	A	4: 149,202,276 (GRCm39)	V227I	probably benign	Het
Dnaaf5	T	A	5: 139,159,909 (GRCm39)	H602Q	probably benign	Het
Efcab7	G	T	4: 99,761,902 (GRCm39)	K397N	probably damaging	Het
Egfl6	C	T	X: 165,319,235 (GRCm39)	V379I	probably benign	Het
Fadd	G	A	7: 144,134,384 (GRCm39)	T167I	probably benign	Het
Fam184b	T	C	5: 45,796,484 (GRCm39)	D33G	probably damaging	Het
Fam237b	C	T	5: 5,625,549 (GRCm39)	Q82*	probably null	Het
Fam83h	G	A	15: 75,877,976 (GRCm39)	R141W	probably damaging	Het
Fanci	G	A	7: 79,076,954 (GRCm39)	R564Q	probably damaging	Het
Fat3	A	T	9: 15,908,054 (GRCm39)	D2649E	possibly damaging	Het
Fcrl1	A	G	3: 87,291,918 (GRCm39)	T46A	possibly damaging	Het
Fig4	C	T	10: 41,143,763 (GRCm39)	G232D	probably benign	Het
Frem3	A	T	8: 81,341,145 (GRCm39)	H1146L	probably damaging	Het
Fyttd1	T	A	16: 32,715,472 (GRCm39)	F133L	probably benign	Het
Gata2	A	G	6: 88,179,505 (GRCm39)	N326D	probably damaging	Het
Hdac1-ps	A	T	17: 78,799,075 (GRCm39)	Y22F	probably benign	Het
Heatr5b	C	T	17: 79,141,524 (GRCm39)		probably null	Het
Hfm1	T	A	5: 107,066,329 (GRCm39)	D33V	possibly damaging	Het
Hmcn2	G	A	2: 31,292,450 (GRCm39)	A2447T	possibly damaging	Het
Hs3st3b1	A	G	11: 63,812,331 (GRCm39)	F128S	probably benign	Het
Hs3st6	G	T	17: 24,972,226 (GRCm39)	R56L	possibly damaging	Het
Hsd17b3	C	T	13: 64,212,171 (GRCm39)	M168I	probably damaging	Het
Ighv1-34	G	T	12: 114,814,885 (GRCm39)	D92E	possibly damaging	Het
Itgb4	A	T	11: 115,885,171 (GRCm39)	I1018F	possibly damaging	Het
Itih3	T	C	14: 30,637,605 (GRCm39)	D518G	possibly damaging	Het
Kcnma1	C	T	14: 23,501,666 (GRCm39)		probably null	Het
Klhl11	A	T	11: 100,363,506 (GRCm39)	S17T	probably benign	Het
Lilra6	T	C	7: 3,917,521 (GRCm39)	E158G	possibly damaging	Het
Loxl1	A	G	9: 58,220,037 (GRCm39)	W45R	probably damaging	Het
Lratd1	T	C	12: 14,200,190 (GRCm39)	N179S	probably benign	Het
Lrfn1	G	T	7: 28,166,255 (GRCm39)	V550F	probably damaging	Het
Lrp1	T	C	10: 127,409,368 (GRCm39)	K1584E	probably benign	Het
Lrp4	C	G	2: 91,338,914 (GRCm39)	P1782A	possibly damaging	Het
Map3k1	A	G	13: 111,885,478 (GRCm39)	S1480P	probably damaging	Het
Mark4	G	T	7: 19,160,015 (GRCm39)	N748K	probably benign	Het
Mrpl38	G	A	11: 116,025,900 (GRCm39)	T142M	probably damaging	Het
Mrpl41	T	C	2: 24,864,481 (GRCm39)	T64A	probably benign	Het
Mrps2	G	A	2: 28,359,764 (GRCm39)	R207K	possibly damaging	Het
Myo5a	A	T	9: 75,099,726 (GRCm39)	E96D		Het
Nbas	A	T	12: 13,633,417 (GRCm39)	E2274V	probably damaging	Het
Or4x13	T	C	2: 90,231,338 (GRCm39)	F111S	probably damaging	Het
Or51a8	T	C	7: 102,549,652 (GRCm39)	I26T	probably benign	Het
Or51ac3	T	C	7: 103,213,850 (GRCm39)	D212G	probably damaging	Het
Or5m10b	T	C	2: 85,699,278 (GRCm39)	L114P	possibly damaging	Het
Or5m10b	T	A	2: 85,699,819 (GRCm39)	N294K	probably damaging	Het
Or6c35	T	A	10: 129,169,368 (GRCm39)	I206N	possibly damaging	Het
Pcdhga12	T	A	18: 37,900,288 (GRCm39)	D373E	probably damaging	Het
Pgd	A	C	4: 149,235,596 (GRCm39)	F395V	probably damaging	Het
Pi16	A	G	17: 29,538,363 (GRCm39)	M59V	possibly damaging	Het
Pik3r5	A	T	11: 68,381,720 (GRCm39)	T255S	probably benign	Het
Plch2	A	T	4: 155,068,516 (GRCm39)	V1370E	probably benign	Het
Plec	A	G	15: 76,067,153 (GRCm39)	I1309T	unknown	Het
Ppp2r3d	A	T	9: 124,440,831 (GRCm38)	S22R		Het
Pramel26	A	G	4: 143,542,609 (GRCm39)	C4R	probably damaging	Het
Ptk2b	C	A	14: 66,413,154 (GRCm39)	E342*	probably null	Het
Ptpn23	T	C	9: 110,215,226 (GRCm39)		probably null	Het
Rfx8	A	C	1: 39,722,850 (GRCm39)	S256A	probably damaging	Het
Rnf115	C	T	3: 96,665,337 (GRCm39)	T69I	probably damaging	Het
Rnf213	A	G	11: 119,370,457 (GRCm39)	Y4753C		Het
S100a10	A	C	3: 93,471,590 (GRCm39)	D58A	possibly damaging	Het
Serpina12	T	C	12: 104,004,317 (GRCm39)	K105R	probably benign	Het
Slc20a2	G	A	8: 23,028,900 (GRCm39)	G124S	probably damaging	Het
Stab2	ACC	AC	10: 86,692,561 (GRCm39)		probably null	Het
Stard9	C	A	2: 120,526,635 (GRCm39)	T964N	probably benign	Het
Stxbp5	T	A	10: 9,774,938 (GRCm39)	I72F	probably damaging	Het
Sumf2	T	A	5: 129,891,482 (GRCm39)	M282K	possibly damaging	Het
Tle7	T	C	8: 110,837,580 (GRCm39)	F375L	probably damaging	Het
Tmem135	G	C	7: 88,797,186 (GRCm39)	L357V	probably benign	Het
Tmem185b	G	T	1: 119,454,613 (GRCm39)	V125L	probably benign	Het
Tmtc1	A	G	6: 148,144,714 (GRCm39)	M887T	probably damaging	Het
Tnfaip8	C	T	18: 50,223,512 (GRCm39)	Q83*	probably null	Het
Tnks	A	G	8: 35,306,089 (GRCm39)	V1162A	probably damaging	Het
Tomm70a	T	A	16: 56,961,072 (GRCm39)	Y342N	possibly damaging	Het
Tpo	T	A	12: 30,125,875 (GRCm39)	D828V	probably damaging	Het
Ugt8a	A	T	3: 125,708,338 (GRCm39)	N257K	probably damaging	Het
Unc13d	AATGCCTCCCATGCC	AATGCCTCCCATGCCTCCCATGCC	11: 115,958,998 (GRCm39)		probably benign	Het
Vim	T	A	2: 13,579,703 (GRCm39)	M154K	probably damaging	Het
Virma	G	T	4: 11,486,045 (GRCm39)	M1I	probably null	Het
Zfp715	A	T	7: 42,950,653 (GRCm39)	M100K	probably benign	Het
Zfp748	A	T	13: 67,690,647 (GRCm39)	C204*	probably null	Het
Zmynd8	A	T	2: 165,680,772 (GRCm39)	D236E	probably damaging	Het

Other mutations in Gm7298

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0030:Gm7298	UTSW	6	121,751,009 (GRCm39)	missense	probably benign
R4978:Gm7298	UTSW	6	121,710,076 (GRCm39)	critical splice donor site	probably null
R4980:Gm7298	UTSW	6	121,736,198 (GRCm39)	splice site	probably null
R6000:Gm7298	UTSW	6	121,742,038 (GRCm39)	missense	possibly damaging	0.91
R6160:Gm7298	UTSW	6	121,741,886 (GRCm39)	missense	probably benign	0.28
R6180:Gm7298	UTSW	6	121,737,782 (GRCm39)	missense	probably benign	0.01
R6243:Gm7298	UTSW	6	121,756,096 (GRCm39)	missense	possibly damaging	0.88
R6266:Gm7298	UTSW	6	121,759,663 (GRCm39)	missense	probably damaging	1.00
R6268:Gm7298	UTSW	6	121,756,032 (GRCm39)	missense	possibly damaging	0.83
R6363:Gm7298	UTSW	6	121,765,565 (GRCm39)	missense	probably damaging	1.00
R6364:Gm7298	UTSW	6	121,756,402 (GRCm39)	missense	possibly damaging	0.90
R6527:Gm7298	UTSW	6	121,746,669 (GRCm39)	missense	probably benign	0.01
R6538:Gm7298	UTSW	6	121,753,132 (GRCm39)	missense	probably damaging	0.98
R6801:Gm7298	UTSW	6	121,752,768 (GRCm39)	missense	probably benign	0.03
R6884:Gm7298	UTSW	6	121,737,480 (GRCm39)	missense	possibly damaging	0.74
R6935:Gm7298	UTSW	6	121,744,653 (GRCm39)	missense	probably benign	0.02
R7051:Gm7298	UTSW	6	121,751,993 (GRCm39)	critical splice donor site	probably null
R7144:Gm7298	UTSW	6	121,738,546 (GRCm39)	missense	probably damaging	0.99
R7178:Gm7298	UTSW	6	121,762,855 (GRCm39)	missense	probably damaging	0.98
R7398:Gm7298	UTSW	6	121,758,912 (GRCm39)	missense	probably benign	0.02
R7706:Gm7298	UTSW	6	121,712,570 (GRCm39)	missense	probably damaging	0.96
R7793:Gm7298	UTSW	6	121,737,563 (GRCm39)	critical splice donor site	probably null
R7829:Gm7298	UTSW	6	121,742,297 (GRCm39)	missense	probably damaging	1.00
R7877:Gm7298	UTSW	6	121,759,741 (GRCm39)	nonsense	probably null
R8010:Gm7298	UTSW	6	121,712,542 (GRCm39)	missense	probably benign
R8167:Gm7298	UTSW	6	121,761,414 (GRCm39)	nonsense	probably null
R8188:Gm7298	UTSW	6	121,763,537 (GRCm39)	critical splice acceptor site	probably null
R8248:Gm7298	UTSW	6	121,764,402 (GRCm39)	missense	probably benign	0.02
R8669:Gm7298	UTSW	6	121,742,002 (GRCm39)	missense	probably benign
R8806:Gm7298	UTSW	6	121,761,641 (GRCm39)	synonymous	silent
R8867:Gm7298	UTSW	6	121,748,788 (GRCm39)	missense	probably benign
R8907:Gm7298	UTSW	6	121,741,817 (GRCm39)	missense	probably benign	0.10
R8930:Gm7298	UTSW	6	121,742,030 (GRCm39)	missense	probably benign	0.01
R8932:Gm7298	UTSW	6	121,742,030 (GRCm39)	missense	probably benign	0.01
R8947:Gm7298	UTSW	6	121,757,553 (GRCm39)	missense	possibly damaging	0.62
R9016:Gm7298	UTSW	6	121,758,800 (GRCm39)	missense	possibly damaging	0.96
R9040:Gm7298	UTSW	6	121,764,438 (GRCm39)	missense	probably benign	0.20
R9069:Gm7298	UTSW	6	121,761,393 (GRCm39)	missense	probably benign
R9154:Gm7298	UTSW	6	121,756,436 (GRCm39)	missense	probably damaging	1.00
R9273:Gm7298	UTSW	6	121,756,604 (GRCm39)	intron	probably benign
R9371:Gm7298	UTSW	6	121,744,541 (GRCm39)	missense	probably benign	0.02
R9372:Gm7298	UTSW	6	121,748,746 (GRCm39)	missense	probably benign	0.21
R9490:Gm7298	UTSW	6	121,751,083 (GRCm39)	missense	probably benign	0.00
Z1176:Gm7298	UTSW	6	121,741,834 (GRCm39)	missense	possibly damaging	0.48
Z1176:Gm7298	UTSW	6	121,741,829 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- ACCTTGACTGTGAGGTTAAATGC -3'
(R):5'- GGTTGTCTGACTTACATAATGGGTC -3'

Sequencing Primer
(F):5'- ACTGTGAGGTTAAATGCTATCCG -3'
(R):5'- ACTTACATAATGGGTCAGTGTTTG -3'

Posted On

2022-10-06