Mutagenetix > Incidental Mutation

Incidental Mutation 'R9654:Vmn2r59'

727263

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R9654 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41661216-41708405 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 41693217 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 461 (N461S)

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

probably benign
Transcript: ENSMUST00000168489
AA Change: N461S

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032
AA Change: N461S

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930407I10Rik	T	C	15: 81,948,916 (GRCm39)	S938P	possibly damaging	Het
Abca17	G	T	17: 24,536,099 (GRCm39)	H523N	probably benign	Het
Alx1	T	A	10: 102,858,093 (GRCm39)	H202L	probably benign	Het
Amotl1	T	A	9: 14,462,981 (GRCm39)	H744L	probably benign	Het
Ankrd50	A	T	3: 38,511,018 (GRCm39)	S450T	probably benign	Het
Aoc1l1	T	C	6: 48,952,837 (GRCm39)	L254P	probably damaging	Het
Arhgef25	C	T	10: 127,021,955 (GRCm39)	S200N	probably damaging	Het
Arl4c	G	T	1: 88,629,361 (GRCm39)	S9*	probably null	Het
Bod1l	A	G	5: 41,975,707 (GRCm39)	M1869T	probably benign	Het
Btnl6	G	A	17: 34,733,140 (GRCm39)	P241L	probably damaging	Het
Cacng8	C	T	7: 3,443,002 (GRCm39)	R88W	probably damaging	Het
Ccdc154	C	T	17: 25,386,684 (GRCm39)	T262I	possibly damaging	Het
Clmn	C	A	12: 104,748,193 (GRCm39)	E451D	probably damaging	Het
Dnah14	A	T	1: 181,593,904 (GRCm39)	I3416L	probably benign	Het
Dnah17	A	G	11: 117,927,156 (GRCm39)		probably null	Het
Dnah3	T	A	7: 119,641,396 (GRCm39)	K1175*	probably null	Het
Dock8	C	T	19: 25,124,710 (GRCm39)	R1009W	probably damaging	Het
Fhip1a	G	T	3: 85,579,532 (GRCm39)	T891K	probably damaging	Het
Fkbp15	A	G	4: 62,230,553 (GRCm39)	V720A	probably benign	Het
Fryl	G	T	5: 73,275,801 (GRCm39)	P121Q	probably benign	Het
H2-Q6	C	T	17: 35,644,185 (GRCm39)	R56C	probably damaging	Het
Hbs1l	T	C	10: 21,183,604 (GRCm39)	V115A	possibly damaging	Het
Heatr5a	G	A	12: 52,005,778 (GRCm39)	P66S	probably damaging	Het
Hsd17b4	A	G	18: 50,272,533 (GRCm39)	D44G	probably benign	Het
Idh3a	T	C	9: 54,497,182 (GRCm39)	V41A	probably benign	Het
Itih1	T	A	14: 30,664,870 (GRCm39)	K37M	probably damaging	Het
Lama1	A	G	17: 68,101,266 (GRCm39)	T1920A		Het
Ltn1	G	C	16: 87,207,227 (GRCm39)	D904E	probably benign	Het
Map6	T	C	7: 98,986,166 (GRCm39)	I893T	probably damaging	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Mr1	T	C	1: 155,013,430 (GRCm39)	H49R	possibly damaging	Het
Myh2	T	C	11: 67,088,171 (GRCm39)	V1929A	probably benign	Het
Ncoa4	C	A	14: 31,896,465 (GRCm39)	P230Q	probably benign	Het
Npy4r	T	A	14: 33,869,081 (GRCm39)	Q69L	probably damaging	Het
Nup210l	C	T	3: 90,107,173 (GRCm39)	P1570L	probably benign	Het
Nus1	T	G	10: 52,294,130 (GRCm39)	L98R	possibly damaging	Het
Or10ag55-ps1	C	T	2: 87,115,071 (GRCm39)	L146F	probably benign	Het
Or14c42-ps1	T	A	7: 86,210,950 (GRCm39)	N3K	unknown	Het
Or2w4	T	C	13: 21,795,915 (GRCm39)	T75A	possibly damaging	Het
Or4c11b	T	C	2: 88,625,263 (GRCm39)	L179S	probably damaging	Het
Or5k17	T	A	16: 58,746,752 (GRCm39)	I61F	probably benign	Het
Pcdha11	A	C	18: 37,145,333 (GRCm39)	T475P	probably damaging	Het
Pex5l	C	A	3: 33,010,827 (GRCm39)	A384S	probably benign	Het
Pgk2	T	A	17: 40,518,651 (GRCm39)	D259V	probably damaging	Het
Rhbdf1	T	C	11: 32,166,028 (GRCm39)	M52V	probably benign	Het
Rsph6a	T	A	7: 18,799,332 (GRCm39)	L321Q	probably damaging	Het
Sugp1	T	A	8: 70,522,656 (GRCm39)	M452K	probably damaging	Het
Sult2a6	T	G	7: 13,956,445 (GRCm39)	D272A	probably benign	Het
Tlr6	A	T	5: 65,112,697 (GRCm39)	L70Q	probably damaging	Het
Tmem178b	A	T	6: 40,222,534 (GRCm39)	Y83F	probably benign	Het
Tmprss4	C	T	9: 45,090,700 (GRCm39)		probably null	Het
Trpv4	A	G	5: 114,764,887 (GRCm39)	L709P	probably benign	Het
Utp15	G	A	13: 98,385,668 (GRCm39)	T519M	probably benign	Het
Zap70	T	C	1: 36,818,327 (GRCm39)	V338A	probably benign	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	41,661,488 (GRCm39)	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	41,661,983 (GRCm39)	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	41,695,593 (GRCm39)	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	41,661,817 (GRCm39)	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	41,661,655 (GRCm39)	missense	probably benign
IGL03346:Vmn2r59	APN	7	41,693,253 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	41,708,340 (GRCm39)	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	41,661,862 (GRCm39)	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	41,695,205 (GRCm39)	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	41,695,725 (GRCm39)	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	41,661,722 (GRCm39)	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	41,696,432 (GRCm39)	nonsense	probably null
R0370:Vmn2r59	UTSW	7	41,662,150 (GRCm39)	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	41,695,916 (GRCm39)	splice site	probably benign
R0465:Vmn2r59	UTSW	7	41,696,332 (GRCm39)	missense	probably benign
R0487:Vmn2r59	UTSW	7	41,696,528 (GRCm39)	nonsense	probably null
R0576:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	41,708,308 (GRCm39)	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	41,661,218 (GRCm39)	makesense	probably null
R1387:Vmn2r59	UTSW	7	41,695,521 (GRCm39)	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	41,695,133 (GRCm39)	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	41,695,629 (GRCm39)	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	41,695,251 (GRCm39)	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	41,693,203 (GRCm39)	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	41,708,326 (GRCm39)	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2257:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2441:Vmn2r59	UTSW	7	41,695,570 (GRCm39)	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	41,693,190 (GRCm39)	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	41,661,370 (GRCm39)	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	41,695,744 (GRCm39)	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	41,670,732 (GRCm39)	intron	probably benign
R4357:Vmn2r59	UTSW	7	41,661,644 (GRCm39)	missense	probably damaging	1.00
R4445:Vmn2r59	UTSW	7	41,691,874 (GRCm39)	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	41,695,497 (GRCm39)	missense	possibly damaging	0.93
R4587:Vmn2r59	UTSW	7	41,695,648 (GRCm39)	missense	probably benign	0.00
R4616:Vmn2r59	UTSW	7	41,661,862 (GRCm39)	missense	probably benign
R4653:Vmn2r59	UTSW	7	41,693,228 (GRCm39)	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	41,661,686 (GRCm39)	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	41,695,218 (GRCm39)	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	41,693,077 (GRCm39)	missense	probably benign
R5045:Vmn2r59	UTSW	7	41,695,496 (GRCm39)	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	41,691,834 (GRCm39)	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	41,696,247 (GRCm39)	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	41,695,105 (GRCm39)	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	41,695,318 (GRCm39)	missense	probably benign	0.27
R5616:Vmn2r59	UTSW	7	41,708,191 (GRCm39)	splice site	probably null
R5625:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	41,695,468 (GRCm39)	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	41,695,491 (GRCm39)	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	41,661,749 (GRCm39)	nonsense	probably null
R6196:Vmn2r59	UTSW	7	41,661,679 (GRCm39)	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	41,691,835 (GRCm39)	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	41,693,177 (GRCm39)	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	41,661,392 (GRCm39)	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	41,693,171 (GRCm39)	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	41,693,277 (GRCm39)	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	41,695,188 (GRCm39)	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	41,695,233 (GRCm39)	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	41,661,443 (GRCm39)	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	41,708,336 (GRCm39)	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	41,695,196 (GRCm39)	nonsense	probably null
R7867:Vmn2r59	UTSW	7	41,661,707 (GRCm39)	missense	probably damaging	1.00
R7975:Vmn2r59	UTSW	7	41,693,199 (GRCm39)	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	41,696,256 (GRCm39)	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	41,661,521 (GRCm39)	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	41,661,247 (GRCm39)	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	41,693,127 (GRCm39)	missense
R9135:Vmn2r59	UTSW	7	41,693,125 (GRCm39)	missense	probably benign	0.33
R9234:Vmn2r59	UTSW	7	41,661,907 (GRCm39)	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	41,695,286 (GRCm39)	nonsense	probably null
R9432:Vmn2r59	UTSW	7	41,696,254 (GRCm39)	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	41,695,590 (GRCm39)	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	41,661,299 (GRCm39)	missense	probably damaging	1.00
R9741:Vmn2r59	UTSW	7	41,708,209 (GRCm39)	missense	probably damaging	0.99
X0025:Vmn2r59	UTSW	7	41,695,365 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	41,661,838 (GRCm39)	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	41,691,941 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGAGAAGTCACACCTCACCTTTG -3'
(R):5'- TTTGCTGGAACTGTGACAAAC -3'

Sequencing Primer
(F):5'- TGTAAACCCTGTAGGCCACTG -3'
(R):5'- TGTGACAAACAGCTGTCCTG -3'

Posted On

2022-10-06