Incidental Mutation 'R9656:Wisp2'
ID 727306
Institutional Source Beutler Lab
Gene Symbol Wisp2
Ensembl Gene ENSMUSG00000027656
Gene Name WNT1 inducible signaling pathway protein 2
Synonyms rCop1, Crgr4, CCN5
MMRRC Submission
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R9656 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 163820861-163833146 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 163829065 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 164 (D164G)
Ref Sequence ENSEMBL: ENSMUSP00000029188 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029188]
AlphaFold no structure available at present
Predicted Effect probably benign
Transcript: ENSMUST00000029188
AA Change: D164G

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)
SMART Domains Protein: ENSMUSP00000029188
Gene: ENSMUSG00000027656
AA Change: D164G

signal peptide 1 23 N/A INTRINSIC
IB 24 93 1.67e-16 SMART
VWC 100 163 5.9e-16 SMART
TSP1 195 239 9.68e-3 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.2%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele are viabe and overtly normal with no adult bone phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abhd13 G A 8: 9,987,991 G196E possibly damaging Het
Atad5 G T 11: 80,089,716 probably benign Het
Atxn2 C T 5: 121,783,998 T635I possibly damaging Het
Bbs10 T A 10: 111,299,684 N219K probably benign Het
Bsn T A 9: 108,117,208 T623S probably benign Het
Btnl9 T C 11: 49,169,181 D580G probably damaging Het
Cckar A G 5: 53,699,976 L364P probably damaging Het
Cfap46 A T 7: 139,655,900 M631K Het
Col1a1 T A 11: 94,948,546 S1006T unknown Het
Cpe T C 8: 64,594,946 Y428C probably damaging Het
Cyp2d26 C A 15: 82,792,858 V122L probably benign Het
Fam114a1 T C 5: 65,005,903 I181T probably benign Het
Fam83b A G 9: 76,545,581 V68A probably benign Het
Fanca A C 8: 123,304,743 V403G probably benign Het
Gm17359 T A 3: 79,405,876 F86I probably benign Het
Gm597 G T 1: 28,777,455 H499N probably benign Het
Grin2a T C 16: 9,579,607 H872R possibly damaging Het
Grpel2 G T 18: 61,726,290 R4S probably benign Het
Gstm1 T C 3: 108,017,756 Y7C probably damaging Het
Hace1 A T 10: 45,671,449 I497F probably benign Het
Hspg2 T C 4: 137,551,885 V3093A probably benign Het
Ighv3-4 A T 12: 114,253,675 F99I possibly damaging Het
Ints3 T C 3: 90,392,532 M963V probably null Het
Krt12 T C 11: 99,418,645 E263G Het
L1td1 T C 4: 98,733,986 F262L probably benign Het
Lrp2bp A G 8: 46,013,121 H110R probably benign Het
Mcm5 C T 8: 75,117,540 S313F probably benign Het
Ncoa6 A G 2: 155,432,926 I135T probably damaging Het
Nfatc3 G A 8: 106,104,134 R695H probably damaging Het
Nkx1-2 T A 7: 132,599,499 K10* probably null Het
Olfr1278 A T 2: 111,292,288 T7S probably benign Het
Olfr1444 A G 19: 12,861,883 Y36C probably damaging Het
Olfr1464-ps1 T C 19: 13,282,671 H129R possibly damaging Het
Olfr314 G A 11: 58,786,809 A192T possibly damaging Het
Otog A G 7: 46,310,143 T719A probably damaging Het
Prkdc T G 16: 15,799,954 I3216M probably benign Het
Pygm A G 19: 6,388,157 D252G probably benign Het
Rae1 T A 2: 173,012,797 Y332* probably null Het
Rbm12 A T 2: 156,098,201 D50E unknown Het
Rbm6 G A 9: 107,779,579 R1000W probably damaging Het
Rd3l A T 12: 111,980,180 V54E possibly damaging Het
Rft1 T C 14: 30,682,757 V370A probably benign Het
Rfx8 T C 1: 39,670,519 I449M probably benign Het
Satb1 A T 17: 51,805,236 S117T possibly damaging Het
Slc24a2 T C 4: 87,049,907 Y370C probably damaging Het
Slc5a4b C A 10: 76,108,557 V85L probably damaging Het
Snrpn A T 7: 59,985,967 V95E possibly damaging Het
Spata33 A T 8: 123,222,019 N130I possibly damaging Het
Tap1 A G 17: 34,193,551 S521G probably damaging Het
Tbc1d13 C T 2: 30,142,417 T180I possibly damaging Het
Tgm7 G A 2: 121,109,710 probably benign Het
Tiam1 A G 16: 89,867,571 M583T probably damaging Het
Ttn C T 2: 76,885,013 E7912K unknown Het
Vmn1r43 A T 6: 89,870,458 H15Q possibly damaging Het
Zfp329 A T 7: 12,810,490 V369E probably damaging Het
Zfp467 T A 6: 48,442,669 E35V possibly damaging Het
Other mutations in Wisp2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01447:Wisp2 APN 2 163829022 missense probably damaging 1.00
BB002:Wisp2 UTSW 2 163829041 missense possibly damaging 0.82
BB012:Wisp2 UTSW 2 163829041 missense possibly damaging 0.82
R0336:Wisp2 UTSW 2 163832322 missense probably damaging 0.98
R0600:Wisp2 UTSW 2 163825313 missense probably damaging 1.00
R1241:Wisp2 UTSW 2 163829077 missense unknown
R1779:Wisp2 UTSW 2 163828986 missense probably damaging 1.00
R2921:Wisp2 UTSW 2 163832346 missense probably benign 0.11
R2923:Wisp2 UTSW 2 163832346 missense probably benign 0.11
R4049:Wisp2 UTSW 2 163828984 missense probably damaging 1.00
R4344:Wisp2 UTSW 2 163828986 missense probably damaging 1.00
R5409:Wisp2 UTSW 2 163825238 missense probably damaging 1.00
R5529:Wisp2 UTSW 2 163825359 critical splice donor site probably null
R5663:Wisp2 UTSW 2 163825253 missense probably damaging 1.00
R6401:Wisp2 UTSW 2 163829026 missense probably benign 0.45
R6685:Wisp2 UTSW 2 163828948 missense possibly damaging 0.87
R7242:Wisp2 UTSW 2 163828852 missense probably benign 0.27
R7925:Wisp2 UTSW 2 163829041 missense possibly damaging 0.82
R8066:Wisp2 UTSW 2 163828942 missense probably damaging 1.00
R8701:Wisp2 UTSW 2 163828866 missense probably damaging 1.00
R8962:Wisp2 UTSW 2 163825240 nonsense probably null
R9215:Wisp2 UTSW 2 163829046 missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2022-10-06