Mutagenetix > Incidental Mutation

Incidental Mutation 'R9657:Virma'

727380

Institutional Source

Beutler Lab

Gene Symbol

Virma

Ensembl Gene

ENSMUSG00000040720

Gene Name

vir like m6A methyltransferase associated

Synonyms

1110037F02Rik

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9657 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

11485958-11550684 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 11544898 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 1569 (E1569G)

Ref Sequence

ENSEMBL: ENSMUSP00000058078 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059914] [ENSMUST00000108307]

AlphaFold

A2AIV2

Predicted Effect

probably damaging
Transcript: ENSMUST00000059914
AA Change: E1569G

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000058078
Gene: ENSMUSG00000040720
AA Change: E1569G

Domain	Start	End	E-Value	Type
low complexity region	139	153	N/A	INTRINSIC
low complexity region	172	198	N/A	INTRINSIC
low complexity region	236	267	N/A	INTRINSIC
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1008	1020	N/A	INTRINSIC
low complexity region	1112	1124	N/A	INTRINSIC
low complexity region	1224	1232	N/A	INTRINSIC
low complexity region	1443	1458	N/A	INTRINSIC
low complexity region	1460	1474	N/A	INTRINSIC
low complexity region	1618	1634	N/A	INTRINSIC
low complexity region	1684	1697	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
low complexity region	1796	1808	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000108307
AA Change: E1619G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103943
Gene: ENSMUSG00000040720
AA Change: E1619G

Domain	Start	End	E-Value	Type
Pfam:VIR_N	5	266	2e-110	PFAM
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1058	1070	N/A	INTRINSIC
low complexity region	1162	1174	N/A	INTRINSIC
low complexity region	1274	1282	N/A	INTRINSIC
low complexity region	1493	1508	N/A	INTRINSIC
low complexity region	1510	1524	N/A	INTRINSIC
low complexity region	1668	1684	N/A	INTRINSIC
low complexity region	1734	1747	N/A	INTRINSIC
low complexity region	1800	1807	N/A	INTRINSIC
low complexity region	1846	1858	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,243,379 (GRCm39)	D1747E	probably benign	Het
Acad11	T	A	9: 103,953,035 (GRCm39)	I88N	possibly damaging	Het
Atp2b4	T	C	1: 133,656,478 (GRCm39)	E724G	probably damaging	Het
Bcl2a1b	T	C	9: 89,081,599 (GRCm39)	S63P	probably damaging	Het
Blzf1	T	C	1: 164,134,023 (GRCm39)	S9G	probably benign	Het
Ccdc121rt1	T	C	1: 181,338,104 (GRCm39)	T283A	probably benign	Het
Cdh22	T	C	2: 164,965,715 (GRCm39)	D488G	probably benign	Het
Cep290	A	G	10: 100,351,003 (GRCm39)	E688G	possibly damaging	Het
Cldn10	A	C	14: 119,025,781 (GRCm39)	E71D	probably benign	Het
Clip2	C	T	5: 134,533,616 (GRCm39)	R487Q	probably benign	Het
Cyp3a16	T	C	5: 145,386,979 (GRCm39)	D337G	probably null	Het
Dnah5	A	T	15: 28,410,089 (GRCm39)	D3654V	probably damaging	Het
Dock1	T	C	7: 134,339,429 (GRCm39)	S100P	possibly damaging	Het
Hdgfl2	T	C	17: 56,405,978 (GRCm39)	V488A	unknown	Het
Itih2	T	C	2: 10,107,686 (GRCm39)	T627A	probably damaging	Het
Katnip	A	G	7: 125,441,956 (GRCm39)	S648G	probably benign	Het
Klhl33	T	C	14: 51,134,117 (GRCm39)	D144G	probably benign	Het
Lca5l	G	A	16: 95,974,953 (GRCm39)	Q324*	probably null	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Mical2	A	G	7: 111,921,806 (GRCm39)	T542A	probably benign	Het
Moxd1	T	A	10: 24,128,485 (GRCm39)	V179E	probably benign	Het
Myh7b	A	G	2: 155,455,963 (GRCm39)	Y116C	probably damaging	Het
Myoc	C	T	1: 162,467,229 (GRCm39)	R133*	probably null	Het
Nol3	A	T	8: 106,005,641 (GRCm39)	I12F	probably damaging	Het
Nrap	A	G	19: 56,352,377 (GRCm39)	V605A	probably benign	Het
Or5w13	T	C	2: 87,524,121 (GRCm39)	Y35C	probably damaging	Het
Or9m2	A	C	2: 87,821,310 (GRCm39)	N285T	probably damaging	Het
Pag1	G	T	3: 9,769,791 (GRCm39)	S52R	probably damaging	Het
Pbxip1	A	G	3: 89,355,056 (GRCm39)	D525G	probably benign	Het
Pla2g4f	A	G	2: 120,135,138 (GRCm39)	F437L	probably benign	Het
Podn	A	C	4: 107,884,231 (GRCm39)	I86S	probably damaging	Het
Postn	A	G	3: 54,290,820 (GRCm39)	T676A	probably benign	Het
Ppp1r1c	A	G	2: 79,638,718 (GRCm39)	E104G	probably benign	Het
Prss53	T	C	7: 127,486,238 (GRCm39)	T436A	probably damaging	Het
Psme4	A	T	11: 30,788,980 (GRCm39)	E1127D	probably benign	Het
Ptpn18	C	A	1: 34,512,473 (GRCm39)	A426E	possibly damaging	Het
Ptprz1	T	G	6: 23,042,377 (GRCm39)	C2044G	possibly damaging	Het
Rad54l2	C	A	9: 106,581,372 (GRCm39)	V850L	probably damaging	Het
Ranbp9	A	G	13: 43,557,155 (GRCm39)	I28T	unknown	Het
Rapgef2	A	T	3: 78,999,191 (GRCm39)	V527E	probably damaging	Het
Rere	C	A	4: 150,699,390 (GRCm39)	P825T	unknown	Het
Rundc3a	A	G	11: 102,291,578 (GRCm39)	T349A	probably benign	Het
Scn2a	T	C	2: 65,566,032 (GRCm39)	F1352S	probably damaging	Het
Senp7	T	A	16: 55,944,295 (GRCm39)	C206*	probably null	Het
Serac1	C	T	17: 6,119,658 (GRCm39)	V91I	probably benign	Het
Serpina1f	G	A	12: 103,656,050 (GRCm39)	Q393*	probably null	Het
Slc8a1	T	C	17: 81,955,244 (GRCm39)	E598G	probably damaging	Het
Slmap	A	T	14: 26,151,013 (GRCm39)	H518Q	probably benign	Het
Spg11	G	A	2: 121,910,781 (GRCm39)	R1199C	probably damaging	Het
Tada1	T	C	1: 166,214,312 (GRCm39)	S104P	possibly damaging	Het
Tbck	A	G	3: 132,421,451 (GRCm39)	D186G	probably damaging	Het
Tdrd9	A	C	12: 112,002,824 (GRCm39)	R824S	possibly damaging	Het
Tmem161a	T	C	8: 70,630,260 (GRCm39)		probably null	Het
Trim55	G	A	3: 19,728,671 (GRCm39)	G494D	possibly damaging	Het
Ttc17	T	A	2: 94,237,010 (GRCm39)	M1L	probably benign	Het
Ttn	C	T	2: 76,715,357 (GRCm39)	E7912K	unknown	Het
Ugt3a1	A	C	15: 9,280,133 (GRCm39)	S32R	probably damaging	Het
Vgll3	T	C	16: 65,636,343 (GRCm39)	S220P	probably benign	Het
Vmn1r54	C	G	6: 90,246,984 (GRCm39)	F299L	probably benign	Het
Vmn2r114	A	G	17: 23,510,690 (GRCm39)	S597P	probably damaging	Het
Wdr7	A	G	18: 64,057,918 (GRCm39)	D1249G	probably damaging	Het
Wdr97	A	G	15: 76,245,476 (GRCm39)	Y1271C		Het
Yju2b	T	C	8: 84,987,084 (GRCm39)	K138E	possibly damaging	Het
Ypel4	A	G	2: 84,568,068 (GRCm39)	Y108C	probably damaging	Het
Zfp770	A	G	2: 114,027,766 (GRCm39)	V101A	probably damaging	Het
Zfyve9	A	T	4: 108,575,729 (GRCm39)	C451S	probably damaging	Het

Other mutations in Virma

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Virma	APN	4	11,519,424 (GRCm39)	splice site	probably benign
IGL00477:Virma	APN	4	11,519,006 (GRCm39)	missense	probably damaging	0.99
IGL01293:Virma	APN	4	11,521,114 (GRCm39)	missense	probably damaging	1.00
IGL01410:Virma	APN	4	11,518,929 (GRCm39)	nonsense	probably null
IGL01531:Virma	APN	4	11,528,753 (GRCm39)	missense	probably damaging	1.00
IGL01672:Virma	APN	4	11,527,792 (GRCm39)	missense	probably damaging	1.00
IGL01724:Virma	APN	4	11,528,672 (GRCm39)	missense	probably damaging	1.00
IGL01747:Virma	APN	4	11,526,877 (GRCm39)	missense	probably damaging	1.00
IGL01776:Virma	APN	4	11,527,792 (GRCm39)	missense	probably damaging	1.00
IGL02064:Virma	APN	4	11,513,163 (GRCm39)	missense	possibly damaging	0.87
IGL02243:Virma	APN	4	11,546,031 (GRCm39)	missense	probably damaging	1.00
IGL02244:Virma	APN	4	11,546,031 (GRCm39)	missense	probably damaging	1.00
IGL02445:Virma	APN	4	11,527,029 (GRCm39)	missense	probably damaging	0.97
IGL02546:Virma	APN	4	11,494,804 (GRCm39)	missense	probably damaging	0.99
IGL02807:Virma	APN	4	11,507,079 (GRCm39)	splice site	probably benign
IGL02967:Virma	APN	4	11,514,096 (GRCm39)	missense	probably benign	0.01
IGL03211:Virma	APN	4	11,548,770 (GRCm39)	nonsense	probably null
IGL03242:Virma	APN	4	11,527,669 (GRCm39)	missense	possibly damaging	0.70
IGL03256:Virma	APN	4	11,542,207 (GRCm39)	splice site	probably benign
IGL03327:Virma	APN	4	11,518,984 (GRCm39)	missense	probably benign	0.00
IGL03346:Virma	APN	4	11,518,984 (GRCm39)	missense	probably benign	0.00
PIT4802001:Virma	UTSW	4	11,546,008 (GRCm39)	missense	probably damaging	0.99
R0142:Virma	UTSW	4	11,548,783 (GRCm39)	missense	probably benign	0.04
R0355:Virma	UTSW	4	11,528,626 (GRCm39)	nonsense	probably null
R0522:Virma	UTSW	4	11,519,416 (GRCm39)	critical splice donor site	probably null
R0600:Virma	UTSW	4	11,498,769 (GRCm39)	missense	probably damaging	0.99
R1435:Virma	UTSW	4	11,528,621 (GRCm39)	missense	probably damaging	1.00
R1489:Virma	UTSW	4	11,521,164 (GRCm39)	missense	probably damaging	1.00
R1568:Virma	UTSW	4	11,528,776 (GRCm39)	missense	probably damaging	0.99
R1616:Virma	UTSW	4	11,544,954 (GRCm39)	missense	probably damaging	1.00
R1655:Virma	UTSW	4	11,494,786 (GRCm39)	missense	probably damaging	1.00
R1695:Virma	UTSW	4	11,494,814 (GRCm39)	missense	probably damaging	0.98
R1835:Virma	UTSW	4	11,540,511 (GRCm39)	missense	probably benign	0.02
R1951:Virma	UTSW	4	11,513,907 (GRCm39)	missense	probably benign	0.00
R1991:Virma	UTSW	4	11,519,242 (GRCm39)	missense	probably benign	0.06
R2145:Virma	UTSW	4	11,548,726 (GRCm39)	splice site	probably benign
R2172:Virma	UTSW	4	11,527,843 (GRCm39)	missense	possibly damaging	0.82
R2217:Virma	UTSW	4	11,544,924 (GRCm39)	missense	probably damaging	1.00
R2218:Virma	UTSW	4	11,544,924 (GRCm39)	missense	probably damaging	1.00
R2248:Virma	UTSW	4	11,518,927 (GRCm39)	missense	probably damaging	1.00
R2342:Virma	UTSW	4	11,501,316 (GRCm39)	missense	probably damaging	1.00
R3424:Virma	UTSW	4	11,513,177 (GRCm39)	nonsense	probably null
R4397:Virma	UTSW	4	11,513,901 (GRCm39)	missense	possibly damaging	0.81
R4449:Virma	UTSW	4	11,498,828 (GRCm39)	critical splice donor site	probably null
R4660:Virma	UTSW	4	11,513,505 (GRCm39)	missense	probably damaging	1.00
R4698:Virma	UTSW	4	11,528,636 (GRCm39)	missense	probably damaging	0.99
R4878:Virma	UTSW	4	11,544,971 (GRCm39)	missense	probably damaging	1.00
R4937:Virma	UTSW	4	11,521,147 (GRCm39)	nonsense	probably null
R5031:Virma	UTSW	4	11,542,116 (GRCm39)	nonsense	probably null
R5040:Virma	UTSW	4	11,528,746 (GRCm39)	missense	probably benign	0.01
R5061:Virma	UTSW	4	11,494,840 (GRCm39)	missense	possibly damaging	0.95
R5091:Virma	UTSW	4	11,519,392 (GRCm39)	missense	probably benign	0.00
R5137:Virma	UTSW	4	11,546,297 (GRCm39)	missense	probably damaging	1.00
R5262:Virma	UTSW	4	11,539,926 (GRCm39)	missense	probably benign	0.01
R5297:Virma	UTSW	4	11,494,819 (GRCm39)	missense	probably damaging	1.00
R5730:Virma	UTSW	4	11,542,154 (GRCm39)	missense	probably benign	0.44
R5818:Virma	UTSW	4	11,513,319 (GRCm39)	missense	possibly damaging	0.92
R5835:Virma	UTSW	4	11,514,036 (GRCm39)	missense	probably damaging	1.00
R6125:Virma	UTSW	4	11,521,172 (GRCm39)	missense	probably damaging	0.98
R6197:Virma	UTSW	4	11,505,498 (GRCm39)	missense	probably damaging	0.96
R6222:Virma	UTSW	4	11,527,820 (GRCm39)	missense	probably damaging	1.00
R6793:Virma	UTSW	4	11,539,968 (GRCm39)	missense	probably damaging	1.00
R7028:Virma	UTSW	4	11,519,249 (GRCm39)	missense	possibly damaging	0.50
R7356:Virma	UTSW	4	11,513,595 (GRCm39)	missense	probably damaging	0.99
R7383:Virma	UTSW	4	11,514,026 (GRCm39)	missense	probably damaging	0.98
R7391:Virma	UTSW	4	11,508,099 (GRCm39)	missense	probably damaging	0.99
R7425:Virma	UTSW	4	11,546,211 (GRCm39)	missense	possibly damaging	0.95
R7556:Virma	UTSW	4	11,518,927 (GRCm39)	missense	probably damaging	1.00
R7715:Virma	UTSW	4	11,513,016 (GRCm39)	splice site	probably null
R7715:Virma	UTSW	4	11,549,682 (GRCm39)	missense	probably damaging	1.00
R7986:Virma	UTSW	4	11,540,023 (GRCm39)	missense	probably benign	0.01
R7990:Virma	UTSW	4	11,513,983 (GRCm39)	missense	probably benign	0.00
R8048:Virma	UTSW	4	11,539,918 (GRCm39)	nonsense	probably null
R8050:Virma	UTSW	4	11,528,643 (GRCm39)	missense	probably benign	0.22
R8165:Virma	UTSW	4	11,542,128 (GRCm39)	missense	probably benign	0.00
R8412:Virma	UTSW	4	11,521,261 (GRCm39)	critical splice donor site	probably null
R8544:Virma	UTSW	4	11,516,949 (GRCm39)	missense	probably benign
R8551:Virma	UTSW	4	11,513,397 (GRCm39)	missense	probably damaging	1.00
R8699:Virma	UTSW	4	11,528,678 (GRCm39)	missense	probably benign	0.04
R8739:Virma	UTSW	4	11,540,643 (GRCm39)	critical splice donor site	probably null
R8950:Virma	UTSW	4	11,519,047 (GRCm39)	nonsense	probably null
R9015:Virma	UTSW	4	11,540,494 (GRCm39)	missense	probably benign	0.27
R9038:Virma	UTSW	4	11,526,922 (GRCm39)	missense	possibly damaging	0.93
R9115:Virma	UTSW	4	11,498,744 (GRCm39)	missense	probably benign	0.15
R9294:Virma	UTSW	4	11,513,507 (GRCm39)	nonsense	probably null
R9404:Virma	UTSW	4	11,513,626 (GRCm39)	missense	probably benign	0.17
R9477:Virma	UTSW	4	11,528,753 (GRCm39)	missense	probably damaging	1.00
R9532:Virma	UTSW	4	11,507,078 (GRCm39)	critical splice donor site	probably null
R9649:Virma	UTSW	4	11,486,045 (GRCm39)	start codon destroyed	probably null	0.08
R9780:Virma	UTSW	4	11,513,442 (GRCm39)	missense	possibly damaging	0.75
R9800:Virma	UTSW	4	11,546,007 (GRCm39)	missense	probably damaging	0.99
X0020:Virma	UTSW	4	11,486,055 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGTGACTTCCTGAAAAGATAGAGG -3'
(R):5'- CACCAGTCACTTCCTGAAATTATTC -3'

Sequencing Primer
(F):5'- GGCAGTTTGTGTAGTGAAAG -3'
(R):5'- AGGTTAGCCTGGTCTAAACAGTCC -3'

Posted On

2022-10-06