Mutagenetix > Incidental Mutation

Incidental Mutation 'R9659:Or4m1'

727471

Institutional Source

Beutler Lab

Gene Symbol

Or4m1

Ensembl Gene

ENSMUSG00000045306

Gene Name

olfactory receptor family 4 subfamily M member 1

Synonyms

Olfr734, GA_x6K02T2PMLR-6013665-6012724, MOR242-1

MMRRC Submission

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.528) question?

Stock #

R9659 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

50557293-50558325 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 50558181 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 37 (F37S)

Ref Sequence

ENSEMBL: ENSMUSP00000150732 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050928] [ENSMUST00000217152]

AlphaFold

Q8VFT4

Predicted Effect

probably benign
Transcript: ENSMUST00000050928
AA Change: F37S

PolyPhen 2 Score 0.098 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000057376
Gene: ENSMUSG00000045306
AA Change: F37S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.3e-39	PFAM
Pfam:7tm_1	41	302	4.1e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217152
AA Change: F37S

PolyPhen 2 Score 0.098 (Sensitivity: 0.93; Specificity: 0.85)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A330070K13Rik	T	C	5: 130,407,876 (GRCm39)	I112V	unknown	Het
Adcy7	A	G	8: 89,045,733 (GRCm39)	T572A	probably benign	Het
Atad5	G	T	11: 79,980,542 (GRCm39)		probably benign	Het
C2cd2	A	G	16: 97,723,473 (GRCm39)	S15P	possibly damaging	Het
Cabp1	T	C	5: 115,311,187 (GRCm39)	D289G	possibly damaging	Het
Ccdc14	A	G	16: 34,541,913 (GRCm39)	I545V	probably damaging	Het
Cep295	C	A	9: 15,233,846 (GRCm39)	A2317S	probably benign	Het
Cerkl	T	C	2: 79,223,322 (GRCm39)	D133G	possibly damaging	Het
Cited4	T	G	4: 120,524,543 (GRCm39)	C182G	probably damaging	Het
Col6a5	T	A	9: 105,811,034 (GRCm39)	K828N	unknown	Het
Ear2	A	G	14: 44,340,705 (GRCm39)	Y121C	probably damaging	Het
Ect2l	C	T	10: 18,041,347 (GRCm39)	C369Y	possibly damaging	Het
Elapor1	G	A	3: 108,377,297 (GRCm39)	T387I	possibly damaging	Het
Ep300	A	G	15: 81,505,273 (GRCm39)	Y631C	unknown	Het
Ephb4	T	G	5: 137,363,743 (GRCm39)	Y583D	probably damaging	Het
Fbn2	C	G	18: 58,342,654 (GRCm39)	R75P	probably damaging	Het
Gabra2	T	C	5: 71,192,140 (GRCm39)	D63G	probably benign	Het
Gfra1	A	G	19: 58,441,652 (GRCm39)	M93T	probably damaging	Het
Gm21886	AGAGGCCTGCAGACAGTAGGTGCTCACTAGGGCCTGCAGACAGCAGGTGCTCACTGAGGCCTG	AGAGGCCTG	18: 80,132,776 (GRCm39)		probably benign	Het
Gm3604	A	T	13: 62,519,724 (GRCm39)	H10Q	possibly damaging	Het
Helz2	T	C	2: 180,882,025 (GRCm39)	D256G	probably benign	Het
Herc1	A	C	9: 66,307,185 (GRCm39)	I1002L	probably benign	Het
Hpgd	C	G	8: 56,772,075 (GRCm39)	C182W	probably damaging	Het
Htr5b	A	G	1: 121,455,428 (GRCm39)	L164P	possibly damaging	Het
Kmo	T	C	1: 175,486,085 (GRCm39)	F403L	probably damaging	Het
Ly75	C	A	2: 60,168,665 (GRCm39)	D748Y	probably damaging	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Mipep	A	G	14: 61,083,893 (GRCm39)	T595A	probably damaging	Het
Muc6	T	C	7: 141,232,100 (GRCm39)	D968G	probably damaging	Het
Myo18b	T	C	5: 113,022,382 (GRCm39)	T337A	unknown	Het
Nlrp1b	T	A	11: 71,073,132 (GRCm39)	Q237L	possibly damaging	Het
Nr1h4	A	G	10: 89,314,638 (GRCm39)		probably null	Het
Nxph1	A	G	6: 9,247,418 (GRCm39)	T130A	probably damaging	Het
Or10al6	T	G	17: 38,082,880 (GRCm39)	F112C	probably damaging	Het
Or1l4	T	A	2: 37,091,897 (GRCm39)	C215S	possibly damaging	Het
Or5p4	T	C	7: 107,680,745 (GRCm39)	V248A	probably damaging	Het
Or8g21	A	G	9: 38,906,296 (GRCm39)	V145A	possibly damaging	Het
Pcsk5	A	G	19: 17,455,245 (GRCm39)	Y1062H	probably benign	Het
Plch1	T	C	3: 63,681,136 (GRCm39)	I164V	probably benign	Het
Plekhh2	T	A	17: 84,854,892 (GRCm39)	M42K	possibly damaging	Het
Polr2a	T	C	11: 69,625,654 (GRCm39)	Y1832C	unknown	Het
Prpf4	G	A	4: 62,334,296 (GRCm39)		probably null	Het
Psg16	T	C	7: 16,824,524 (GRCm39)	S12P	possibly damaging	Het
Ptpn14	T	A	1: 189,587,174 (GRCm39)	M528K	probably benign	Het
Rb1cc1	T	A	1: 6,318,673 (GRCm39)	H697Q	probably benign	Het
Reg3a	T	G	6: 78,360,574 (GRCm39)	C171G	possibly damaging	Het
Slc22a26	G	A	19: 7,763,798 (GRCm39)	P412S	probably benign	Het
Spata31e5	G	T	1: 28,816,536 (GRCm39)	H499N	probably benign	Het
Tcstv2a	T	A	13: 120,725,754 (GRCm39)	D139E	probably damaging	Het
Tlk2	T	C	11: 105,131,263 (GRCm39)	I203T	probably benign	Het
Tmem120b	T	G	5: 123,253,788 (GRCm39)	H279Q	probably damaging	Het
Trip4	T	C	9: 65,740,702 (GRCm39)	E535G	probably benign	Het
Ttn	C	T	2: 76,715,357 (GRCm39)	E7912K	unknown	Het
Ubr5	A	T	15: 37,984,254 (GRCm39)	L2298I		Het
Utp20	A	G	10: 88,653,171 (GRCm39)	L303P	probably damaging	Het
Vcan	A	G	13: 89,839,860 (GRCm39)	Y1895H	probably damaging	Het
Vmn2r2	T	C	3: 64,041,942 (GRCm39)	N258D	possibly damaging	Het
Wdr20rt	A	T	12: 65,273,343 (GRCm39)	S269C	probably damaging	Het
Zfand4	T	G	6: 116,282,588 (GRCm39)	Y54D	probably damaging	Het
Zfp82	C	T	7: 29,755,963 (GRCm39)	R373H	probably damaging	Het
Zmiz2	A	G	11: 6,346,814 (GRCm39)	E141G	probably benign	Het

Other mutations in Or4m1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01140:Or4m1	APN	14	50,557,732 (GRCm39)	missense	probably damaging	0.96
IGL01285:Or4m1	APN	14	50,557,713 (GRCm39)	missense	possibly damaging	0.88
IGL02106:Or4m1	APN	14	50,557,617 (GRCm39)	missense	probably damaging	1.00
IGL02313:Or4m1	APN	14	50,557,473 (GRCm39)	missense	probably damaging	0.99
IGL03125:Or4m1	APN	14	50,558,149 (GRCm39)	missense	probably benign	0.01
R0276:Or4m1	UTSW	14	50,557,636 (GRCm39)	missense	probably benign	0.23
R0547:Or4m1	UTSW	14	50,557,575 (GRCm39)	missense	probably benign	0.06
R0567:Or4m1	UTSW	14	50,558,115 (GRCm39)	missense	probably damaging	0.99
R0927:Or4m1	UTSW	14	50,558,186 (GRCm39)	nonsense	probably null
R1506:Or4m1	UTSW	14	50,557,941 (GRCm39)	missense	probably benign	0.00
R4032:Or4m1	UTSW	14	50,557,767 (GRCm39)	missense	possibly damaging	0.91
R5179:Or4m1	UTSW	14	50,557,993 (GRCm39)	nonsense	probably null
R5401:Or4m1	UTSW	14	50,557,566 (GRCm39)	missense	probably damaging	1.00
R6240:Or4m1	UTSW	14	50,558,043 (GRCm39)	missense	probably benign	0.00
R7752:Or4m1	UTSW	14	50,557,573 (GRCm39)	missense	probably damaging	1.00
R7901:Or4m1	UTSW	14	50,557,573 (GRCm39)	missense	probably damaging	1.00
R8034:Or4m1	UTSW	14	50,558,023 (GRCm39)	missense	probably damaging	1.00
R8260:Or4m1	UTSW	14	50,557,615 (GRCm39)	missense	probably benign	0.09
R8420:Or4m1	UTSW	14	50,558,233 (GRCm39)	missense	probably benign
R9056:Or4m1	UTSW	14	50,557,999 (GRCm39)	missense	probably damaging	0.98
R9128:Or4m1	UTSW	14	50,558,214 (GRCm39)	missense	probably benign	0.08
R9618:Or4m1	UTSW	14	50,557,760 (GRCm39)	nonsense	probably null
R9788:Or4m1	UTSW	14	50,558,181 (GRCm39)	missense	probably benign	0.10
X0064:Or4m1	UTSW	14	50,557,511 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCGGTCAAAAGCCATCACTG -3'
(R):5'- CCCCTGTGTAATCTATTAATGATGC -3'

Sequencing Primer
(F):5'- TGTCAGCAGGAACATCTCAG -3'
(R):5'- GCAAATGATACCACCGTGA -3'

Posted On

2022-10-06