Mutagenetix > Incidental Mutation

Incidental Mutation 'R9661:Tmem26'

727586

Institutional Source

Beutler Lab

Gene Symbol

Tmem26

Ensembl Gene

ENSMUSG00000060044

Gene Name

transmembrane protein 26

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R9661 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

68559576-68618485 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 68559838 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 37 (W37R)

Ref Sequence

ENSEMBL: ENSMUSP00000079789 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080995] [ENSMUST00000218918] [ENSMUST00000219392]

AlphaFold

Q3UP23

Predicted Effect

probably damaging
Transcript: ENSMUST00000080995
AA Change: W37R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000079789
Gene: ENSMUSG00000060044
AA Change: W37R

Domain	Start	End	E-Value	Type
Pfam:Tmem26	3	304	5.6e-125	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000218918
AA Change: W37R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000219392
AA Change: W37R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein containing multiple transmembrane helices. It is a selective surface protein marker of brite/beige adipocytes, which may coexist with classical brown adipocytes in brown adipose tissue. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430033K04Rik	T	A	5: 138,645,451 (GRCm39)	H445Q	possibly damaging	Het
Aadacl4	C	A	4: 144,340,287 (GRCm39)	P4Q	probably damaging	Het
Adgre1	A	G	17: 57,748,368 (GRCm39)	T523A	possibly damaging	Het
Arhgap32	A	G	9: 32,168,531 (GRCm39)	E838G	probably benign	Het
Atad5	G	T	11: 79,980,542 (GRCm39)		probably benign	Het
Card10	C	T	15: 78,683,318 (GRCm39)	R275H	probably damaging	Het
Cdh16	A	G	8: 105,345,612 (GRCm39)	V327A	probably benign	Het
Clip2	C	T	5: 134,533,616 (GRCm39)	R487Q	probably benign	Het
Cttnbp2	T	A	6: 18,429,151 (GRCm39)	Q159L		Het
D630003M21Rik	A	G	2: 158,047,673 (GRCm39)	L732P	possibly damaging	Het
Dgcr8	T	C	16: 18,098,579 (GRCm39)	I308V	possibly damaging	Het
Dhx9	A	G	1: 153,340,393 (GRCm39)	F702L	probably damaging	Het
Dpy19l1	T	C	9: 24,386,730 (GRCm39)	Y188C	probably damaging	Het
Eea1	T	C	10: 95,862,742 (GRCm39)	L888S	probably benign	Het
Elmo1	T	C	13: 20,469,531 (GRCm39)		probably null	Het
Fam186a	T	A	15: 99,842,492 (GRCm39)	I1251F	possibly damaging	Het
Fer1l4	G	A	2: 155,862,336 (GRCm39)	A1779V	probably damaging	Het
Fgfrl1	C	T	5: 108,853,841 (GRCm39)	T407I	probably benign	Het
Gkn1	T	C	6: 87,325,173 (GRCm39)	K79E	probably damaging	Het
Gm11564	C	T	11: 99,706,247 (GRCm39)	R61H	unknown	Het
Gpr107	T	C	2: 31,057,075 (GRCm39)	F56L	probably benign	Het
H2-T22	T	C	17: 36,353,371 (GRCm39)		probably benign	Het
Hs1bp3	T	C	12: 8,367,940 (GRCm39)	V63A	probably damaging	Het
Htr6	A	G	4: 138,801,963 (GRCm39)	I37T	probably damaging	Het
Kifc5b	T	C	17: 27,140,836 (GRCm39)		probably null	Het
Lrfn2	T	C	17: 49,403,650 (GRCm39)	I591T	probably benign	Het
Lrrc49	A	T	9: 60,573,582 (GRCm39)	I300N	probably damaging	Het
Lyst	A	G	13: 13,808,779 (GRCm39)	T150A	probably benign	Het
Myh11	T	C	16: 14,041,857 (GRCm39)	Q722R		Het
Nek9	T	C	12: 85,361,253 (GRCm39)	Q484R	possibly damaging	Het
Nfs1	A	T	2: 155,970,473 (GRCm39)	V280E	probably damaging	Het
Notch3	T	A	17: 32,373,792 (GRCm39)	D472V	probably damaging	Het
Nt5c1b	T	C	12: 10,425,450 (GRCm39)	V273A	probably damaging	Het
Nudt12	T	C	17: 59,316,981 (GRCm39)	D222G	probably benign	Het
Nup98	G	A	7: 101,782,019 (GRCm39)	Q1204*	probably null	Het
Nwd2	A	T	5: 63,957,780 (GRCm39)	Y370F	probably damaging	Het
Oas3	A	T	5: 120,904,230 (GRCm39)	I548N	unknown	Het
Ogfr	A	T	2: 180,233,431 (GRCm39)	N78Y	probably damaging	Het
Or10ak11	A	T	4: 118,687,526 (GRCm39)	L36Q	probably benign	Het
Prkcq	A	T	2: 11,250,141 (GRCm39)	K153*	probably null	Het
Ptpdc1	T	A	13: 48,739,610 (GRCm39)	N607I	probably benign	Het
Rab5c	G	T	11: 100,606,917 (GRCm39)	Q221K	probably benign	Het
Ret	G	A	6: 118,150,437 (GRCm39)	T693I	probably benign	Het
Rnf20	A	G	4: 49,654,556 (GRCm39)	E871G	probably damaging	Het
Rtl1	G	T	12: 109,557,346 (GRCm39)	P1498T	possibly damaging	Het
Septin9	C	G	11: 117,245,751 (GRCm39)	R458G	possibly damaging	Het
Sh2d2a	T	C	3: 87,756,788 (GRCm39)		probably null	Het
Slc14a1	C	A	18: 78,152,807 (GRCm39)	A367S	probably damaging	Het
Slc16a4	T	A	3: 107,213,359 (GRCm39)	D451E	probably benign	Het
Slc36a3	A	G	11: 55,015,984 (GRCm39)	F390S	probably benign	Het
Slc4a2	T	A	5: 24,640,005 (GRCm39)	M576K	probably damaging	Het
Smim14	T	C	5: 65,610,533 (GRCm39)	H88R	possibly damaging	Het
Spata31e5	G	T	1: 28,816,536 (GRCm39)	H499N	probably benign	Het
Sspo	C	T	6: 48,455,272 (GRCm39)	Q3045*	probably null	Het
Supt7l	C	A	5: 31,680,403 (GRCm39)	R3M	probably damaging	Het
Tjap1	A	G	17: 46,571,092 (GRCm39)	W132R	probably damaging	Het
Tut7	T	C	13: 59,937,332 (GRCm39)	D1155G	probably damaging	Het
Vmn1r119	C	T	7: 20,746,224 (GRCm39)	V53M	possibly damaging	Het
Vmn2r54	A	T	7: 12,349,166 (GRCm39)	N805K	probably benign	Het
Zfp638	T	C	6: 83,923,320 (GRCm39)	V706A	probably damaging	Het

Other mutations in Tmem26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00155:Tmem26	APN	10	68,611,184 (GRCm39)	missense	probably damaging	1.00
IGL00471:Tmem26	APN	10	68,614,511 (GRCm39)	missense	possibly damaging	0.78
IGL01301:Tmem26	APN	10	68,614,436 (GRCm39)	missense	probably damaging	1.00
IGL01567:Tmem26	APN	10	68,587,061 (GRCm39)	missense	probably damaging	1.00
IGL02487:Tmem26	APN	10	68,614,563 (GRCm39)	missense	probably benign	0.00
IGL02713:Tmem26	APN	10	68,587,125 (GRCm39)	missense	probably damaging	1.00
IGL02828:Tmem26	APN	10	68,611,215 (GRCm39)	critical splice donor site	probably null
ANU18:Tmem26	UTSW	10	68,614,436 (GRCm39)	missense	probably damaging	1.00
P0027:Tmem26	UTSW	10	68,614,548 (GRCm39)	missense	probably benign	0.00
R1415:Tmem26	UTSW	10	68,614,491 (GRCm39)	missense	possibly damaging	0.93
R1649:Tmem26	UTSW	10	68,587,103 (GRCm39)	missense	probably damaging	1.00
R3871:Tmem26	UTSW	10	68,614,562 (GRCm39)	missense	probably benign	0.01
R5072:Tmem26	UTSW	10	68,611,178 (GRCm39)	missense	probably damaging	1.00
R5239:Tmem26	UTSW	10	68,587,096 (GRCm39)	missense	probably damaging	0.97
R6053:Tmem26	UTSW	10	68,584,314 (GRCm39)	missense	probably benign	0.00
R6607:Tmem26	UTSW	10	68,614,543 (GRCm39)	missense	probably benign	0.00
R6710:Tmem26	UTSW	10	68,559,884 (GRCm39)	missense	probably damaging	1.00
R7378:Tmem26	UTSW	10	68,559,922 (GRCm39)	critical splice donor site	probably null
R9276:Tmem26	UTSW	10	68,614,488 (GRCm39)	missense	possibly damaging	0.58
R9303:Tmem26	UTSW	10	68,559,816 (GRCm39)	nonsense	probably null
R9305:Tmem26	UTSW	10	68,559,816 (GRCm39)	nonsense	probably null
R9716:Tmem26	UTSW	10	68,576,790 (GRCm39)	missense	probably damaging	1.00
T0722:Tmem26	UTSW	10	68,614,548 (GRCm39)	missense	probably benign	0.00
X0003:Tmem26	UTSW	10	68,614,548 (GRCm39)	missense	probably benign	0.00
Z1177:Tmem26	UTSW	10	68,559,793 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGAACTCGGGCTGGCAATC -3'
(R):5'- ACCCGAGACAAGGTTTTGGTTC -3'

Sequencing Primer
(F):5'- AATCACAGTCCCAGGGGTGTC -3'
(R):5'- CCTCCTGCTAAGACCGTGATG -3'

Posted On

2022-10-06