Mutagenetix > Incidental Mutation

Incidental Mutation 'R9665:Prmt9'

727770

Institutional Source

Beutler Lab

Gene Symbol

Prmt9

Ensembl Gene

ENSMUSG00000037134

Gene Name

protein arginine methyltransferase 9

Synonyms

Prmt10

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.226) question?

Stock #

R9665 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

78276026-78307967 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 78307267 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 739 (V739I)

Ref Sequence

ENSEMBL: ENSMUSP00000050181 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056237] [ENSMUST00000118622] [ENSMUST00000210040]

AlphaFold

Q3U3W5

Predicted Effect

probably benign
Transcript: ENSMUST00000056237
AA Change: V739I

PolyPhen 2 Score 0.180 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000050181
Gene: ENSMUSG00000037134
AA Change: V739I

Domain	Start	End	E-Value	Type
low complexity region	5	21	N/A	INTRINSIC
Pfam:TPR_11	67	132	1.2e-7	PFAM
Pfam:TPR_2	102	134	7.9e-5	PFAM
Pfam:PrmA	168	257	2.5e-10	PFAM
internal_repeat_1	585	836	1.37e-10	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000118622
AA Change: V739I

PolyPhen 2 Score 0.281 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000112692
Gene: ENSMUSG00000037134
AA Change: V739I

Domain	Start	End	E-Value	Type
low complexity region	5	21	N/A	INTRINSIC
Pfam:TPR_2	102	134	3e-5	PFAM
Pfam:PrmA	168	257	4.9e-10	PFAM
internal_repeat_1	585	836	1.05e-10	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000210040

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503E14Rik	T	C	14: 44,407,796 (GRCm39)	E79G	probably damaging	Het
4930583I09Rik	T	A	17: 65,141,288 (GRCm39)	Q78L	unknown	Het
9330159F19Rik	G	T	10: 29,103,344 (GRCm39)	V642F	probably benign	Het
Abca9	T	A	11: 110,006,280 (GRCm39)	Q1275L	probably benign	Het
Abca9	G	T	11: 110,006,281 (GRCm39)	Q1275K	probably benign	Het
Ager	C	T	17: 34,819,090 (GRCm39)	P313S	probably benign	Het
Ap4m1	T	A	5: 138,171,273 (GRCm39)	N98K	probably benign	Het
Arhgef12	T	G	9: 42,929,650 (GRCm39)	K215T	possibly damaging	Het
Arhgef4	T	C	1: 34,849,518 (GRCm39)	Y370H	probably benign	Het
Bcat1	T	G	6: 144,994,488 (GRCm39)	H3P	probably benign	Het
Btnl6	T	A	17: 34,732,635 (GRCm39)	Q313L	probably benign	Het
Cad	T	C	5: 31,229,703 (GRCm39)	S1458P	probably benign	Het
Cfap161	A	G	7: 83,442,579 (GRCm39)	V78A	probably benign	Het
Chd2	C	A	7: 73,079,555 (GRCm39)	A1782S	probably benign	Het
Cimip2b	T	A	4: 43,427,554 (GRCm39)	R257*	probably null	Het
Col4a3	A	T	1: 82,668,301 (GRCm39)	T1042S	unknown	Het
Cramp1	A	T	17: 25,196,545 (GRCm39)	N856K	probably damaging	Het
Crispld2	G	A	8: 120,760,316 (GRCm39)	R414H	probably benign	Het
Ctsb	T	A	14: 63,370,917 (GRCm39)		probably null	Het
Cyp2c69	T	C	19: 39,839,504 (GRCm39)	D373G	possibly damaging	Het
Dnah3	A	G	7: 119,644,981 (GRCm39)	V1100A	probably benign	Het
Dnajb6	A	T	5: 29,971,374 (GRCm39)	L225F	probably damaging	Het
Dscc1	A	T	15: 54,946,837 (GRCm39)	I296K	unknown	Het
Epb41l2	A	G	10: 25,317,798 (GRCm39)	D105G	probably benign	Het
Evpl	G	A	11: 116,123,497 (GRCm39)	R292C	probably damaging	Het
Flnc	T	C	6: 29,455,447 (GRCm39)	I2032T	probably damaging	Het
Fryl	T	A	5: 73,222,299 (GRCm39)	D2094V	probably damaging	Het
Gigyf2	A	G	1: 87,331,457 (GRCm39)	S202G	unknown	Het
Hdgfl1	A	T	13: 26,953,812 (GRCm39)	M87K	probably benign	Het
Htra3	T	A	5: 35,836,654 (GRCm39)	I13F	unknown	Het
Igf2r	T	C	17: 12,913,027 (GRCm39)	N1774D	probably benign	Het
Ighj1	A	T	12: 113,393,446 (GRCm39)	Y3N		Het
Incenp	T	C	19: 9,871,329 (GRCm39)	H100R	unknown	Het
Klrb1c	A	G	6: 128,760,625 (GRCm39)		probably null	Het
Lmbrd1	T	A	1: 24,732,065 (GRCm39)	V94D	probably damaging	Het
Lmna	A	T	3: 88,389,793 (GRCm39)	S611T	probably benign	Het
Lsp1	A	C	7: 142,044,142 (GRCm39)	I296L	probably benign	Het
Mdga1	A	T	17: 30,051,991 (GRCm39)	I892N	probably damaging	Het
Micu3	A	G	8: 40,828,666 (GRCm39)	E351G	probably benign	Het
Mrpl35	A	G	6: 71,795,704 (GRCm39)	V61A	probably benign	Het
Nhsl1	A	G	10: 18,401,599 (GRCm39)	K908E	possibly damaging	Het
Nlk	A	G	11: 78,481,753 (GRCm39)	L236P		Het
Nwd1	T	A	8: 73,401,106 (GRCm39)	H735Q	probably damaging	Het
Or1e1b-ps1	A	T	11: 73,845,656 (GRCm39)	I47F	probably benign	Het
Or5d47	A	G	2: 87,804,596 (GRCm39)	S138P	possibly damaging	Het
Or5w17	A	T	2: 87,583,671 (GRCm39)	I222N	probably damaging	Het
Or7h8	T	A	9: 20,124,402 (GRCm39)	Y252*	probably null	Het
Otof	T	A	5: 30,584,895 (GRCm39)	S70C	probably benign	Het
Pcdh20	A	T	14: 88,707,162 (GRCm39)	V46D	probably benign	Het
Pjvk	A	G	2: 76,487,827 (GRCm39)	T240A	probably benign	Het
Pou2f1	A	G	1: 165,703,600 (GRCm39)	S697P	probably damaging	Het
Prkn	T	A	17: 11,286,062 (GRCm39)	D18E	possibly damaging	Het
Prr14	A	G	7: 127,073,091 (GRCm39)	D146G	probably benign	Het
Rai14	T	G	15: 10,574,803 (GRCm39)	E747D	probably damaging	Het
Rapgef4	A	T	2: 72,036,018 (GRCm39)	T504S	probably benign	Het
Rilpl2	C	A	5: 124,616,240 (GRCm39)		probably benign	Het
Ror2	A	T	13: 53,439,561 (GRCm39)	M1K	probably null	Het
Shisa9	A	G	16: 12,085,446 (GRCm39)	T352A	probably benign	Het
Slc35a4	A	G	18: 36,813,651 (GRCm39)	N33S	probably benign	Het
Slx4	G	T	16: 3,806,890 (GRCm39)	L518I	probably benign	Het
Speer1i	A	G	5: 11,092,852 (GRCm39)	K132R		Het
Spon2	T	C	5: 33,374,702 (GRCm39)	T35A	probably benign	Het
Syne3	A	C	12: 104,924,247 (GRCm39)	L471R	probably benign	Het
Tas2r115	A	G	6: 132,714,390 (GRCm39)	L187S	probably benign	Het
Tbc1d30	G	T	10: 121,102,886 (GRCm39)	S715R	possibly damaging	Het
Tdrd1	A	G	19: 56,819,572 (GRCm39)	E24G	probably benign	Het
Tekt2	C	T	4: 126,217,444 (GRCm39)	R207H	probably damaging	Het
Tjp1	A	G	7: 64,962,644 (GRCm39)	S1178P	probably benign	Het
Upf2	A	G	2: 6,051,715 (GRCm39)	S1126G	unknown	Het
Vmn1r128	G	T	7: 21,083,362 (GRCm39)	C22F	probably damaging	Het
Vmn2r95	G	T	17: 18,660,607 (GRCm39)	V340F	probably damaging	Het
Vps13c	A	T	9: 67,863,025 (GRCm39)	K3014*	probably null	Het
Wdr75	T	C	1: 45,843,013 (GRCm39)	V126A	unknown	Het
Yeats4	A	T	10: 117,053,343 (GRCm39)	Y139*	probably null	Het
Zc3h13	A	G	14: 75,567,989 (GRCm39)	D1094G	probably damaging	Het
Zfp143	C	G	7: 109,692,414 (GRCm39)	I608M	probably damaging	Het
Zfp772	A	T	7: 7,212,793 (GRCm39)	M1K	probably null	Het
Zfp827	A	G	8: 79,906,756 (GRCm39)	M923V	possibly damaging	Het

Other mutations in Prmt9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01801:Prmt9	APN	8	78,289,069 (GRCm39)	missense	probably damaging	1.00
IGL02306:Prmt9	APN	8	78,287,447 (GRCm39)	missense	probably benign	0.06
IGL02971:Prmt9	APN	8	78,291,698 (GRCm39)	missense	probably benign	0.00
1mM(1):Prmt9	UTSW	8	78,282,393 (GRCm39)	missense	probably benign	0.01
R0004:Prmt9	UTSW	8	78,282,411 (GRCm39)	missense	possibly damaging	0.81
R0928:Prmt9	UTSW	8	78,307,805 (GRCm39)	missense	probably damaging	1.00
R1328:Prmt9	UTSW	8	78,299,283 (GRCm39)	missense	possibly damaging	0.86
R1777:Prmt9	UTSW	8	78,291,737 (GRCm39)	missense	probably benign	0.05
R1826:Prmt9	UTSW	8	78,282,303 (GRCm39)	nonsense	probably null
R1925:Prmt9	UTSW	8	78,303,968 (GRCm39)	missense	possibly damaging	0.52
R3855:Prmt9	UTSW	8	78,294,894 (GRCm39)	missense	probably benign	0.22
R3856:Prmt9	UTSW	8	78,294,894 (GRCm39)	missense	probably benign	0.22
R4089:Prmt9	UTSW	8	78,299,174 (GRCm39)	missense	probably benign	0.00
R4963:Prmt9	UTSW	8	78,282,358 (GRCm39)	missense	probably damaging	1.00
R5196:Prmt9	UTSW	8	78,291,626 (GRCm39)	missense	probably benign	0.30
R5413:Prmt9	UTSW	8	78,298,638 (GRCm39)	missense	possibly damaging	0.95
R5975:Prmt9	UTSW	8	78,287,647 (GRCm39)	intron	probably benign
R6271:Prmt9	UTSW	8	78,304,092 (GRCm39)	missense	probably damaging	0.96
R7023:Prmt9	UTSW	8	78,276,086 (GRCm39)	start gained	probably benign
R7107:Prmt9	UTSW	8	78,294,880 (GRCm39)	missense	possibly damaging	0.62
R7159:Prmt9	UTSW	8	78,282,393 (GRCm39)	missense	probably benign	0.01
R7209:Prmt9	UTSW	8	78,291,627 (GRCm39)	missense	probably benign	0.32
R7770:Prmt9	UTSW	8	78,285,814 (GRCm39)	splice site	probably null
R7819:Prmt9	UTSW	8	78,294,973 (GRCm39)	missense	probably benign	0.11
R7959:Prmt9	UTSW	8	78,287,594 (GRCm39)	missense	probably damaging	1.00
R9106:Prmt9	UTSW	8	78,276,358 (GRCm39)	missense	probably benign	0.05
R9301:Prmt9	UTSW	8	78,282,374 (GRCm39)	nonsense	probably null
R9368:Prmt9	UTSW	8	78,285,663 (GRCm39)	missense	probably benign	0.00
X0027:Prmt9	UTSW	8	78,287,512 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- GAAAGTGTAGTGTGTTTCCTTCCAAG -3'
(R):5'- CCCGTGTAAATAAAGATAGTACCTTCC -3'

Sequencing Primer
(F):5'- TGTAAGTAACAAAGCACACCAAGGTG -3'
(R):5'- CCTTCCGTTTAAGGTAAATGTCTTG -3'

Posted On

2022-10-06