Incidental Mutation 'R9667:Trim25'
ID 727874
Institutional Source Beutler Lab
Gene Symbol Trim25
Ensembl Gene ENSMUSG00000000275
Gene Name tripartite motif-containing 25
Synonyms Zfp147, estrogen-responsive finger protein
MMRRC Submission
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R9667 (G1)
Quality Score 225.009
Status Not validated
Chromosome 11
Chromosomal Location 88890202-88911119 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 88907188 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 516 (I516F)
Ref Sequence ENSEMBL: ENSMUSP00000103528 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000000284] [ENSMUST00000100627] [ENSMUST00000107896]
AlphaFold Q61510
PDB Structure PRY-SPRY domain of Trim25 [X-RAY DIFFRACTION]
Predicted Effect probably damaging
Transcript: ENSMUST00000000284
AA Change: I508F

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000000284
Gene: ENSMUSG00000000275
AA Change: I508F

DomainStartEndE-ValueType
RING 13 53 8.04e-9 SMART
Blast:SPEC 189 288 5e-34 BLAST
PDB:4LTB|B 189 380 7e-69 PDB
PRY 453 505 3.44e-17 SMART
SPRY 506 626 9.62e-31 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000100627
SMART Domains Protein: ENSMUSP00000098192
Gene: ENSMUSG00000000275

DomainStartEndE-ValueType
RING 13 53 8.04e-9 SMART
Blast:BBOX 151 186 3e-8 BLAST
Blast:SPEC 189 288 2e-37 BLAST
PDB:4LTB|B 189 380 2e-71 PDB
Predicted Effect probably damaging
Transcript: ENSMUST00000107896
AA Change: I516F

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000103528
Gene: ENSMUSG00000000275
AA Change: I516F

DomainStartEndE-ValueType
RING 13 53 8.04e-9 SMART
Blast:SPEC 189 288 3e-34 BLAST
PDB:4LTB|B 189 380 8e-69 PDB
low complexity region 382 393 N/A INTRINSIC
PRY 461 513 3.44e-17 SMART
SPRY 514 634 9.62e-31 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Engineered mutations result in a compromised response to estrogen resulting in functional but small uteri. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 88 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930563M21Rik G A 9: 55,890,645 (GRCm39) Q355* probably null Het
Aacs A T 5: 125,580,691 (GRCm39) N255I possibly damaging Het
Abcf2 A G 5: 24,779,185 (GRCm39) L121P probably damaging Het
Amigo1 A G 3: 108,095,034 (GRCm39) I178V probably benign Het
Ankrd13a T A 5: 114,933,793 (GRCm39) N262K probably damaging Het
Apba2 A G 7: 64,345,062 (GRCm39) E84G possibly damaging Het
Arhgap35 A T 7: 16,296,914 (GRCm39) L717* probably null Het
Aspa A T 11: 73,199,625 (GRCm39) C217* probably null Het
Atp5mc3 A G 2: 73,739,567 (GRCm39) I91T probably damaging Het
Birc6 T C 17: 75,004,420 (GRCm39) M4763T possibly damaging Het
Cc2d2b A G 19: 40,753,927 (GRCm39) Y150C unknown Het
Ccdc180 C T 4: 45,920,861 (GRCm39) Q936* probably null Het
Cdc42bpg A G 19: 6,370,115 (GRCm39) E1103G probably benign Het
Cdca2 T C 14: 67,915,003 (GRCm39) Q752R probably benign Het
Ces1b A T 8: 93,791,637 (GRCm39) S321T probably benign Het
Cfap70 A T 14: 20,490,690 (GRCm39) probably null Het
Cpox C T 16: 58,490,984 (GRCm39) T65I possibly damaging Het
Crocc C T 4: 140,748,988 (GRCm39) E1606K probably damaging Het
Cryzl2 T G 1: 157,316,038 (GRCm39) M337R probably benign Het
Cyp4a29 C A 4: 115,111,630 (GRCm39) A469D probably damaging Het
Dnaaf1 T A 8: 120,306,043 (GRCm39) Y107N possibly damaging Het
Dpp6 T A 5: 27,930,604 (GRCm39) I812N probably damaging Het
Elovl5 A G 9: 77,889,947 (GRCm39) T253A possibly damaging Het
Fancd2 T A 6: 113,530,717 (GRCm39) L450* probably null Het
Fbxo31 C T 8: 122,305,208 (GRCm39) R96H probably damaging Het
Gls A T 1: 52,230,036 (GRCm39) probably null Het
Gm3045 G A 13: 56,577,253 (GRCm39) E134K possibly damaging Het
Gpr158 A G 2: 21,830,054 (GRCm39) I700V probably damaging Het
H2-Aa T C 17: 34,502,295 (GRCm39) I209V probably benign Het
Ifnar2 A G 16: 91,184,984 (GRCm39) D125G probably benign Het
Igsf8 C A 1: 172,145,319 (GRCm39) D278E possibly damaging Het
Inpp5d T C 1: 87,623,128 (GRCm39) I407T probably damaging Het
Iqcd A G 5: 120,744,737 (GRCm39) E355G probably damaging Het
Klra2 T A 6: 131,219,836 (GRCm39) D115V probably benign Het
Kmt2b T C 7: 30,287,784 (GRCm39) E120G unknown Het
Lhx6 A G 2: 35,980,979 (GRCm39) I321T possibly damaging Het
Lmna CAGCACGGTGCGTGAGC CAGC 3: 88,389,857 (GRCm39) probably null Het
Lztr1 T C 16: 17,327,000 (GRCm39) Y37H probably damaging Het
Malrd1 A T 2: 15,570,026 (GRCm39) probably null Het
Mtmr3 C T 11: 4,470,890 (GRCm39) V79I probably damaging Het
Nbr1 A G 11: 101,451,261 (GRCm39) I56M possibly damaging Het
Ncor2 C T 5: 125,125,545 (GRCm39) A580T unknown Het
Ndst3 A G 3: 123,353,866 (GRCm39) I601T possibly damaging Het
Nectin4 C A 1: 171,210,165 (GRCm39) P219Q probably damaging Het
Nfatc4 T C 14: 56,066,964 (GRCm39) V501A probably benign Het
Nisch T C 14: 30,895,646 (GRCm39) T1015A probably damaging Het
Nr2c1 T C 10: 94,017,479 (GRCm39) probably null Het
Nrg2 T C 18: 36,165,430 (GRCm39) E394G probably benign Het
Oas1e A G 5: 120,932,347 (GRCm39) F99L probably benign Het
Oaz3 A T 3: 94,341,835 (GRCm39) Y178* probably null Het
Or1e35 A G 11: 73,798,097 (GRCm39) S74P possibly damaging Het
Or6c69b A C 10: 129,627,022 (GRCm39) S145R probably damaging Het
Pcdhb20 C T 18: 37,637,839 (GRCm39) H122Y probably benign Het
Pdcd2 T A 17: 15,747,535 (GRCm39) M1L probably damaging Het
Phf11 T C 14: 59,482,240 (GRCm39) N171S probably benign Het
Plcd1 C A 9: 118,901,698 (GRCm39) G609W probably damaging Het
Plscr3 T C 11: 69,738,631 (GRCm39) F98L probably benign Het
Ppp1r8 T C 4: 132,570,407 (GRCm39) N6S probably benign Het
Prpf38b G A 3: 108,818,859 (GRCm39) probably benign Het
Rin2 T A 2: 145,702,202 (GRCm39) N299K possibly damaging Het
Serpina1d T G 12: 103,734,299 (GRCm39) T2P probably benign Het
Slc22a16 A G 10: 40,461,125 (GRCm39) D330G probably benign Het
Slit1 G A 19: 41,731,832 (GRCm39) Q6* probably null Het
Spaca7b T A 8: 11,705,681 (GRCm39) E142V probably benign Het
Spag9 T C 11: 93,887,119 (GRCm39) V8A possibly damaging Het
Spsb3 C T 17: 25,105,784 (GRCm39) T44I unknown Het
Srrt T C 5: 137,295,732 (GRCm39) Y563C probably damaging Het
Syne2 A G 12: 75,926,951 (GRCm39) D69G probably damaging Het
Tcf12 A G 9: 71,792,443 (GRCm39) V80A probably benign Het
Tdpoz3 G A 3: 93,733,336 (GRCm39) D4N possibly damaging Het
Tekt2 C T 4: 126,217,444 (GRCm39) R207H probably damaging Het
Tmem132d G A 5: 128,061,375 (GRCm39) T409I possibly damaging Het
Tmem245 G T 4: 56,947,119 (GRCm39) T98K probably damaging Het
Tmem70 A C 1: 16,735,659 (GRCm39) E43A probably benign Het
Trim3 G A 7: 105,267,455 (GRCm39) S308L possibly damaging Het
Ttll9 C T 2: 152,831,989 (GRCm39) R189* probably null Het
Ttpal T C 2: 163,449,596 (GRCm39) probably null Het
Ubl4b A T 3: 107,461,911 (GRCm39) H116Q probably benign Het
Unc80 C A 1: 66,651,287 (GRCm39) T1544K possibly damaging Het
Usp2 T C 9: 44,003,487 (GRCm39) probably null Het
Usp25 T C 16: 76,874,123 (GRCm39) probably null Het
Vmn1r167 C T 7: 23,204,990 (GRCm39) V9I probably benign Het
Vmn2r87 A G 10: 130,314,776 (GRCm39) I270T probably damaging Het
Vps35l G A 7: 118,348,915 (GRCm39) probably null Het
Wdr24 A G 17: 26,046,301 (GRCm39) D515G possibly damaging Het
Wdr81 T C 11: 75,341,650 (GRCm39) T87A Het
Zcchc14 A T 8: 122,331,863 (GRCm39) L500Q unknown Het
Zzef1 A G 11: 72,758,786 (GRCm39) T1242A probably benign Het
Other mutations in Trim25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01707:Trim25 APN 11 88,890,517 (GRCm39) missense probably damaging 0.96
IGL02398:Trim25 APN 11 88,890,630 (GRCm39) missense probably damaging 1.00
IGL03150:Trim25 APN 11 88,890,831 (GRCm39) missense probably damaging 1.00
R0003:Trim25 UTSW 11 88,906,598 (GRCm39) missense probably benign 0.01
R0184:Trim25 UTSW 11 88,890,466 (GRCm39) missense probably damaging 1.00
R0707:Trim25 UTSW 11 88,890,564 (GRCm39) missense probably benign 0.03
R1855:Trim25 UTSW 11 88,906,407 (GRCm39) missense probably benign 0.04
R1936:Trim25 UTSW 11 88,895,576 (GRCm39) missense probably benign 0.03
R2229:Trim25 UTSW 11 88,907,447 (GRCm39) missense probably damaging 0.97
R3401:Trim25 UTSW 11 88,901,707 (GRCm39) missense probably benign
R5159:Trim25 UTSW 11 88,890,358 (GRCm39) missense probably benign 0.20
R5378:Trim25 UTSW 11 88,900,093 (GRCm39) missense probably damaging 1.00
R6149:Trim25 UTSW 11 88,906,362 (GRCm39) missense probably benign 0.00
R6867:Trim25 UTSW 11 88,901,713 (GRCm39) missense probably benign 0.00
R6996:Trim25 UTSW 11 88,890,329 (GRCm39) missense probably benign 0.00
R7055:Trim25 UTSW 11 88,890,750 (GRCm39) missense probably benign
R7310:Trim25 UTSW 11 88,906,608 (GRCm39) missense probably benign 0.03
R7451:Trim25 UTSW 11 88,906,563 (GRCm39) missense possibly damaging 0.76
R7632:Trim25 UTSW 11 88,906,602 (GRCm39) missense probably null 0.91
R7767:Trim25 UTSW 11 88,899,943 (GRCm39) critical splice acceptor site probably null
R8132:Trim25 UTSW 11 88,907,432 (GRCm39) missense probably damaging 0.99
R8785:Trim25 UTSW 11 88,904,340 (GRCm39) missense probably benign 0.00
R8978:Trim25 UTSW 11 88,907,027 (GRCm39) missense probably benign 0.01
R9135:Trim25 UTSW 11 88,899,988 (GRCm39) missense probably benign
R9189:Trim25 UTSW 11 88,901,731 (GRCm39) missense probably benign 0.00
R9348:Trim25 UTSW 11 88,900,167 (GRCm39) nonsense probably null
R9731:Trim25 UTSW 11 88,906,391 (GRCm39) missense probably benign 0.00
X0022:Trim25 UTSW 11 88,906,422 (GRCm39) missense probably benign 0.05
Predicted Primers PCR Primer
(F):5'- AAGCTTTAAAAGTGCAGGCAC -3'
(R):5'- AGAGTCTTCTCCACGTTGTTG -3'

Sequencing Primer
(F):5'- CCTTGTCAAAATTCCCTGGGGG -3'
(R):5'- GTTGTGCCAGGCAGAGATC -3'
Posted On 2022-10-06