Mutagenetix > Incidental Mutation

Incidental Mutation 'R9669:Trav13-1'

727978

Institutional Source

Beutler Lab

Gene Symbol

Trav13-1

Ensembl Gene

ENSMUSG00000076839

Gene Name

T cell receptor alpha variable 13-1

Synonyms

Gm16980

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R9669 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

53782471-53782982 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 53782510 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 14 (T14S)

Ref Sequence

ENSEMBL: ENSMUSP00000100428 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000103651]

AlphaFold

A0A075B644

Predicted Effect

probably benign
Transcript: ENSMUST00000103651
AA Change: T14S

PolyPhen 2 Score 0.068 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000100428
Gene: ENSMUSG00000076839
AA Change: T14S

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
IGv	38	109	1.6e-10	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 22 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adnp	T	C	2: 168,026,918 (GRCm39)	K126E	possibly damaging	Het
Ankrd35	A	G	3: 96,587,797 (GRCm39)	N112S	probably damaging	Het
Bnip3l	G	A	14: 67,246,214 (GRCm39)	P7L	possibly damaging	Het
Ccnjl	C	A	11: 43,476,166 (GRCm39)	T263K	probably benign	Het
Cfhr4	A	T	1: 139,708,872 (GRCm39)	I12N	probably damaging	Het
Cox4i2	C	A	2: 152,602,610 (GRCm39)	N101K	probably damaging	Het
D6Ertd527e	G	C	6: 87,088,839 (GRCm39)	S334T	unknown	Het
Dhx57	T	C	17: 80,553,130 (GRCm39)	I1266V	probably benign	Het
Gm19410	A	G	8: 36,247,493 (GRCm39)	D435G	possibly damaging	Het
Gtf2ird1	A	G	5: 134,408,794 (GRCm39)	Y702H	probably damaging	Het
Igkv10-96	T	C	6: 68,608,957 (GRCm39)	T113A	probably benign	Het
Ngdn	A	G	14: 55,259,339 (GRCm39)	K161R	possibly damaging	Het
Or7d9	T	A	9: 20,197,160 (GRCm39)	L63H	probably damaging	Het
Pabpc4l	A	G	3: 46,401,267 (GRCm39)	S126P	probably damaging	Het
Pcdhgc4	A	G	18: 37,949,241 (GRCm39)	D219G	probably damaging	Het
Plek	A	G	11: 16,944,775 (GRCm39)	F85S	probably benign	Het
Sidt1	A	G	16: 44,102,243 (GRCm39)	Y306H	probably damaging	Het
Tst	T	C	15: 78,289,853 (GRCm39)	I61V	probably benign	Het
Txndc2	T	C	17: 65,945,583 (GRCm39)	E198G	probably damaging	Het
Uba6	T	C	5: 86,268,499 (GRCm39)	I910V	probably benign	Het
Zfyve16	A	G	13: 92,656,007 (GRCm39)	V784A	probably damaging	Het
Zkscan5	A	G	5: 145,142,136 (GRCm39)	H11R	probably benign	Het

Other mutations in Trav13-1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R6376:Trav13-1	UTSW	14	53,782,800 (GRCm39)	missense	probably benign	0.12
R9737:Trav13-1	UTSW	14	53,782,510 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- ATCTCTCAGCGAGGTCTTGC -3'
(R):5'- AGATGGCTGTCACTGCAGTG -3'

Sequencing Primer
(F):5'- AACTCAGCAGGGTGTGGC -3'
(R):5'- TGTCACTGCAGTGTCCACAGAG -3'

Posted On

2022-10-06