Mutagenetix > Incidental Mutation

Incidental Mutation 'R9681:Zfp64'

728533

Institutional Source

Beutler Lab

Gene Symbol

Zfp64

Ensembl Gene

ENSMUSG00000027551

Gene Name

zinc finger protein 64

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.863) question?

Stock #

R9681 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

168735251-168797507 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 168793680 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 22 (V22E)

Ref Sequence

ENSEMBL: ENSMUSP00000085285 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087971] [ENSMUST00000109161] [ENSMUST00000109162]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000087971
AA Change: V22E

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000085285
Gene: ENSMUSG00000027551
AA Change: V22E

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	257	279	3.89e-3	SMART
ZnF_C2H2	285	308	6.32e-3	SMART
ZnF_C2H2	313	335	2.05e-2	SMART
ZnF_C2H2	341	363	1.95e-3	SMART
ZnF_C2H2	369	392	1.36e-2	SMART
ZnF_C2H2	423	445	1.47e-3	SMART
low complexity region	503	522	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000109161
AA Change: V22E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000104789
Gene: ENSMUSG00000027551
AA Change: V22E

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109162
AA Change: V22E

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000104790
Gene: ENSMUSG00000027551
AA Change: V22E

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	297	322	5.2e0	SMART
ZnF_C2H2	328	350	4.17e-3	SMART
ZnF_C2H2	356	378	6.78e-3	SMART
ZnF_C2H2	384	406	2.24e-3	SMART
ZnF_C2H2	412	434	1.79e-2	SMART
ZnF_C2H2	440	463	9.58e-3	SMART
ZnF_C2H2	465	487	3.78e-1	SMART
ZnF_C2H2	493	515	3.89e-3	SMART
ZnF_C2H2	521	544	1.92e-2	SMART
ZnF_C2H2	578	600	5.34e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610030E20Rik	A	G	6: 72,324,450 (GRCm39)	D20G	probably damaging	Het
Acbd3	T	A	1: 180,566,082 (GRCm39)	Y258*	probably null	Het
Adamts1	T	A	16: 85,599,498 (GRCm39)	H34L		Het
Ankmy1	G	T	1: 92,813,882 (GRCm39)	N432K	possibly damaging	Het
Ano1	A	G	7: 144,143,893 (GRCm39)	M966T	possibly damaging	Het
Ark2n	A	T	18: 77,722,989 (GRCm39)	V349D	possibly damaging	Het
C2cd3	A	G	7: 100,023,662 (GRCm39)	T83A	probably benign	Het
Cdk1	T	A	10: 69,178,449 (GRCm39)	D137V	possibly damaging	Het
Cfap299	T	A	5: 98,477,214 (GRCm39)	M1K	probably null	Het
Chia1	A	G	3: 106,037,996 (GRCm39)	Y326C	probably damaging	Het
Ciz1	A	G	2: 32,260,974 (GRCm39)	D295G	possibly damaging	Het
Clrn1	C	T	3: 58,792,251 (GRCm39)	V71I	probably benign	Het
Cr1l	T	C	1: 194,800,149 (GRCm39)	D175G	probably damaging	Het
Crem	A	T	18: 3,268,067 (GRCm39)	V87E	probably damaging	Het
Cyp2b19	T	A	7: 26,466,328 (GRCm39)	L377Q	probably benign	Het
Ddx42	T	A	11: 106,125,679 (GRCm39)	V243D	probably damaging	Het
Dnah14	T	C	1: 181,562,414 (GRCm39)	V2658A	possibly damaging	Het
Dnah3	T	G	7: 119,677,611 (GRCm39)	M437L	probably benign	Het
Flrt2	T	A	12: 95,745,425 (GRCm39)		probably benign	Het
Gm10142	T	A	10: 77,551,880 (GRCm39)	C80*	probably null	Het
Gpr158	A	G	2: 21,831,315 (GRCm39)	E805G	probably damaging	Het
Grwd1	T	C	7: 45,479,473 (GRCm39)	E134G	probably benign	Het
Hdac11	T	A	6: 91,150,068 (GRCm39)	V289D	probably benign	Het
Hsdl1	T	C	8: 120,293,081 (GRCm39)	E118G	possibly damaging	Het
Igdcc4	T	A	9: 65,041,858 (GRCm39)	L1095Q	possibly damaging	Het
Ildr1	T	A	16: 36,528,749 (GRCm39)	C65S	probably damaging	Het
Itgal	T	C	7: 126,929,422 (GRCm39)	F1113S	probably damaging	Het
Itsn2	G	T	12: 4,683,499 (GRCm39)	V341F	unknown	Het
Jak1	G	A	4: 101,019,085 (GRCm39)	R723C	probably damaging	Het
Limch1	A	G	5: 67,126,422 (GRCm39)	T8A	probably damaging	Het
Limd1	T	A	9: 123,345,903 (GRCm39)	C561S	possibly damaging	Het
Map3k19	A	G	1: 127,750,097 (GRCm39)	F1085L	possibly damaging	Het
Mcm7	A	T	5: 138,164,220 (GRCm39)	Y600*	probably null	Het
Mecom	A	T	3: 30,033,803 (GRCm39)	D300E	probably benign	Het
Mfap4	T	A	11: 61,376,925 (GRCm39)	Y51*	probably null	Het
Mug1	T	A	6: 121,833,254 (GRCm39)	N286K	probably benign	Het
Myl2	A	T	5: 122,240,783 (GRCm39)	R40*	probably null	Het
Nom1	A	G	5: 29,642,623 (GRCm39)	S375G	probably damaging	Het
Nrcam	C	A	12: 44,598,133 (GRCm39)	P368Q	probably null	Het
Oca2	G	C	7: 55,943,623 (GRCm39)	Q265H	probably null	Het
Or52a20	T	A	7: 103,366,475 (GRCm39)	F225I	probably damaging	Het
Or52n2c	T	C	7: 104,574,075 (GRCm39)	T299A	probably damaging	Het
Or5k8	T	A	16: 58,644,176 (GRCm39)	N299Y	possibly damaging	Het
Or6c88	A	T	10: 129,406,664 (GRCm39)	T47S	probably damaging	Het
Or7a41	G	A	10: 78,871,577 (GRCm39)	D316N	probably benign	Het
Palmd	T	C	3: 116,717,120 (GRCm39)	E459G	probably benign	Het
Pde1a	G	T	2: 79,695,465 (GRCm39)	A494D	probably benign	Het
Pds5a	T	C	5: 65,808,587 (GRCm39)	Y428C	probably damaging	Het
Pgm2	T	C	5: 64,254,391 (GRCm39)	F59L	probably benign	Het
Plekhm1	A	G	11: 103,258,950 (GRCm39)	V980A	possibly damaging	Het
Polb	T	C	8: 23,118,346 (GRCm39)	D318G	possibly damaging	Het
Rasgef1c	T	A	11: 49,861,040 (GRCm39)	M335K	probably damaging	Het
Rasgrp4	T	C	7: 28,849,687 (GRCm39)	S651P	probably benign	Het
Robo3	A	C	9: 37,334,558 (GRCm39)	I624S	possibly damaging	Het
Robo3	T	A	9: 37,339,087 (GRCm39)	H290L	probably benign	Het
Rps6kl1	T	C	12: 85,183,599 (GRCm39)	H482R	probably damaging	Het
Slc26a9	A	T	1: 131,681,691 (GRCm39)	E168V	probably benign	Het
Slc3a2	T	C	19: 8,691,226 (GRCm39)		probably benign	Het
Slc4a4	A	T	5: 89,102,723 (GRCm39)	K54*	probably null	Het
Slitrk6	A	G	14: 110,988,258 (GRCm39)	L483P	probably damaging	Het
Svep1	T	A	4: 58,084,959 (GRCm39)	N1793I	probably damaging	Het
Ttn	T	C	2: 76,612,723 (GRCm39)	I17119V	possibly damaging	Het
Tulp4	T	C	17: 6,274,500 (GRCm39)	L617P	possibly damaging	Het
Uhrf1	A	G	17: 56,625,083 (GRCm39)	N542S	possibly damaging	Het
Vmn1r151	T	A	7: 22,198,368 (GRCm39)	T246S	probably damaging	Het
Vmn1r57	T	A	7: 5,224,069 (GRCm39)	V198E	probably damaging	Het
Vmn2r99	A	T	17: 19,598,889 (GRCm39)	Q191L	probably damaging	Het
Vrk3	C	T	7: 44,403,356 (GRCm39)	T39M	possibly damaging	Het
Zc3h3	C	A	15: 75,681,470 (GRCm39)	R537L	probably damaging	Het
Zwint	T	C	10: 72,493,112 (GRCm39)	L218P	probably damaging	Het

Other mutations in Zfp64

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00155:Zfp64	APN	2	168,768,601 (GRCm39)	missense	probably benign	0.06
IGL01479:Zfp64	APN	2	168,793,599 (GRCm39)	missense	probably damaging	1.00
IGL02320:Zfp64	APN	2	168,768,118 (GRCm39)	missense	probably damaging	0.98
IGL03411:Zfp64	APN	2	168,793,462 (GRCm39)	splice site	probably null
PIT4362001:Zfp64	UTSW	2	168,767,735 (GRCm39)	missense	probably benign	0.23
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0230:Zfp64	UTSW	2	168,754,150 (GRCm39)	splice site	probably benign
R0325:Zfp64	UTSW	2	168,767,960 (GRCm39)	missense	probably benign	0.00
R0689:Zfp64	UTSW	2	168,777,121 (GRCm39)	splice site	probably benign
R1741:Zfp64	UTSW	2	168,768,238 (GRCm39)	missense	probably benign	0.05
R2054:Zfp64	UTSW	2	168,767,728 (GRCm39)	missense	probably damaging	1.00
R2133:Zfp64	UTSW	2	168,782,663 (GRCm39)	missense	probably damaging	1.00
R2254:Zfp64	UTSW	2	168,768,662 (GRCm39)	missense	probably damaging	0.96
R4093:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4094:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4257:Zfp64	UTSW	2	168,768,298 (GRCm39)	missense	probably damaging	1.00
R4630:Zfp64	UTSW	2	168,768,463 (GRCm39)	missense	possibly damaging	0.94
R4642:Zfp64	UTSW	2	168,776,851 (GRCm39)	missense	probably benign	0.22
R4817:Zfp64	UTSW	2	168,768,032 (GRCm39)	missense	probably benign
R4880:Zfp64	UTSW	2	168,736,297 (GRCm39)	missense	probably damaging	1.00
R5051:Zfp64	UTSW	2	168,768,304 (GRCm39)	missense	probably damaging	0.98
R5155:Zfp64	UTSW	2	168,748,885 (GRCm39)	missense	probably benign	0.17
R5562:Zfp64	UTSW	2	168,767,642 (GRCm39)	missense	probably benign	0.00
R5619:Zfp64	UTSW	2	168,741,735 (GRCm39)	missense	probably damaging	0.97
R5619:Zfp64	UTSW	2	168,741,734 (GRCm39)	missense	probably damaging	0.99
R5620:Zfp64	UTSW	2	168,741,888 (GRCm39)	missense	possibly damaging	0.70
R5919:Zfp64	UTSW	2	168,768,419 (GRCm39)	missense	probably benign	0.05
R6156:Zfp64	UTSW	2	168,768,088 (GRCm39)	missense	probably benign	0.34
R6364:Zfp64	UTSW	2	168,754,186 (GRCm39)	missense	probably damaging	1.00
R6488:Zfp64	UTSW	2	168,777,129 (GRCm39)	critical splice donor site	probably null
R6512:Zfp64	UTSW	2	168,735,997 (GRCm39)	missense	probably benign	0.00
R6588:Zfp64	UTSW	2	168,768,827 (GRCm39)	missense	probably damaging	1.00
R7103:Zfp64	UTSW	2	168,768,357 (GRCm39)	missense	probably benign	0.00
R7313:Zfp64	UTSW	2	168,741,810 (GRCm39)	missense	probably damaging	1.00
R7470:Zfp64	UTSW	2	168,767,731 (GRCm39)	missense	probably damaging	1.00
R7529:Zfp64	UTSW	2	168,735,992 (GRCm39)	missense	probably benign
R7560:Zfp64	UTSW	2	168,767,585 (GRCm39)	missense	probably damaging	1.00
R7963:Zfp64	UTSW	2	168,793,538 (GRCm39)	missense	probably benign	0.32
R8037:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8038:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8825:Zfp64	UTSW	2	168,793,552 (GRCm39)	missense	probably benign
R8840:Zfp64	UTSW	2	168,768,635 (GRCm39)	missense	probably benign
R8891:Zfp64	UTSW	2	168,797,083 (GRCm39)	start codon destroyed	probably null	0.37
R9062:Zfp64	UTSW	2	168,767,747 (GRCm39)	missense	probably benign
R9592:Zfp64	UTSW	2	168,768,118 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATAGTCTGAGTCTCTGAACTGATG -3'
(R):5'- ACACTGCCTTGTAGGACGTG -3'

Sequencing Primer
(F):5'- AGTCTCTGAACTGATGGTCGTTG -3'
(R):5'- TGTAGGACGTGACTCACATCTCG -3'

Posted On

2022-10-06