Mutagenetix > Incidental Mutation

Incidental Mutation 'R9681:Vmn2r99'

728589

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R9681 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19378627 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 191 (Q191L)

Ref Sequence

ENSEMBL: ENSMUSP00000135236 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably damaging
Transcript: ENSMUST00000176107
AA Change: Q191L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: Q191L

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000231989
AA Change: Q191L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610030E20Rik	A	G	6: 72,347,467	D20G	probably damaging	Het
1700007G11Rik	T	A	5: 98,329,355	M1K	probably null	Het
8030462N17Rik	A	T	18: 77,635,293	V349D	possibly damaging	Het
Acbd3	T	A	1: 180,738,517	Y258*	probably null	Het
Adamts1	T	A	16: 85,802,610	H34L		Het
Ankmy1	G	T	1: 92,886,160	N432K	possibly damaging	Het
Ano1	A	G	7: 144,590,156	M966T	possibly damaging	Het
C2cd3	A	G	7: 100,374,455	T83A	probably benign	Het
Cdk1	T	A	10: 69,342,619	D137V	possibly damaging	Het
Chia1	A	G	3: 106,130,680	Y326C	probably damaging	Het
Ciz1	A	G	2: 32,370,962	D295G	possibly damaging	Het
Clrn1	C	T	3: 58,884,830	V71I	probably benign	Het
Cr1l	T	C	1: 195,117,841	D175G	probably damaging	Het
Crem	A	T	18: 3,268,067	V87E	probably damaging	Het
Cyp2b19	T	A	7: 26,766,903	L377Q	probably benign	Het
Ddx42	T	A	11: 106,234,853	V243D	probably damaging	Het
Dnah14	T	C	1: 181,734,849	V2658A	possibly damaging	Het
Dnah3	T	G	7: 120,078,388	M437L	probably benign	Het
Flrt2	T	A	12: 95,778,651		probably benign	Het
Gm10142	T	A	10: 77,716,046	C80*	probably null	Het
Gpr158	A	G	2: 21,826,504	E805G	probably damaging	Het
Grwd1	T	C	7: 45,830,049	E134G	probably benign	Het
Hdac11	T	A	6: 91,173,086	V289D	probably benign	Het
Hsdl1	T	C	8: 119,566,342	E118G	possibly damaging	Het
Igdcc4	T	A	9: 65,134,576	L1095Q	possibly damaging	Het
Ildr1	T	A	16: 36,708,387	C65S	probably damaging	Het
Itgal	T	C	7: 127,330,250	F1113S	probably damaging	Het
Itsn2	G	T	12: 4,633,499	V341F	unknown	Het
Jak1	G	A	4: 101,161,888	R723C	probably damaging	Het
Limch1	A	G	5: 66,969,079	T8A	probably damaging	Het
Limd1	T	A	9: 123,516,838	C561S	possibly damaging	Het
Map3k19	A	G	1: 127,822,360	F1085L	possibly damaging	Het
Mcm7	A	T	5: 138,165,958	Y600*	probably null	Het
Mecom	A	T	3: 29,979,654	D300E	probably benign	Het
Mfap4	T	A	11: 61,486,099	Y51*	probably null	Het
Mug1	T	A	6: 121,856,295	N286K	probably benign	Het
Myl2	A	T	5: 122,102,720	R40*	probably null	Het
Nom1	A	G	5: 29,437,625	S375G	probably damaging	Het
Nrcam	C	A	12: 44,551,350	P368Q	probably null	Het
Oca2	G	C	7: 56,293,875	Q265H	probably null	Het
Olfr175-ps1	T	A	16: 58,823,813	N299Y	possibly damaging	Het
Olfr243	T	A	7: 103,717,268	F225I	probably damaging	Het
Olfr57	G	A	10: 79,035,743	D316N	probably benign	Het
Olfr668	T	C	7: 104,924,868	T299A	probably damaging	Het
Olfr794	A	T	10: 129,570,795	T47S	probably damaging	Het
Palmd	T	C	3: 116,923,471	E459G	probably benign	Het
Pde1a	G	T	2: 79,865,121	A494D	probably benign	Het
Pds5a	T	C	5: 65,651,244	Y428C	probably damaging	Het
Pgm1	T	C	5: 64,097,048	F59L	probably benign	Het
Plekhm1	A	G	11: 103,368,124	V980A	possibly damaging	Het
Polb	T	C	8: 22,628,330	D318G	possibly damaging	Het
Rasgef1c	T	A	11: 49,970,213	M335K	probably damaging	Het
Rasgrp4	T	C	7: 29,150,262	S651P	probably benign	Het
Robo3	A	C	9: 37,423,262	I624S	possibly damaging	Het
Robo3	T	A	9: 37,427,791	H290L	probably benign	Het
Rps6kl1	T	C	12: 85,136,825	H482R	probably damaging	Het
Slc26a9	A	T	1: 131,753,953	E168V	probably benign	Het
Slc3a2	T	C	19: 8,713,862		probably benign	Het
Slc4a4	A	T	5: 88,954,864	K54*	probably null	Het
Slitrk6	A	G	14: 110,750,826	L483P	probably damaging	Het
Svep1	T	A	4: 58,084,959	N1793I	probably damaging	Het
Ttn	T	C	2: 76,782,379	I17119V	possibly damaging	Het
Tulp4	T	C	17: 6,224,225	L617P	possibly damaging	Het
Uhrf1	A	G	17: 56,318,083	N542S	possibly damaging	Het
Vmn1r151	T	A	7: 22,498,943	T246S	probably damaging	Het
Vmn1r57	T	A	7: 5,221,070	V198E	probably damaging	Het
Vrk3	C	T	7: 44,753,932	T39M	possibly damaging	Het
Zc3h3	C	A	15: 75,809,621	R537L	probably damaging	Het
Zfp64	A	T	2: 168,951,760	V22E	probably damaging	Het
Zwint	T	C	10: 72,657,280	L218P	probably damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19378854	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19394256	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19382623	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19393658	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19380115	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19380232	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19378690	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19378223	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19394285	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19394285	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19394343	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19394573	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19379043	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19362259	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19380060	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19362252	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19377945	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19362153	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19378815	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19377991	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19378629	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19378708	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19394373	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19378990	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19378990	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19378570	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19379260	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19393662	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19362135	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19378606	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19379339	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19379269	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19394146	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19377948	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19378980	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19382558	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19382558	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19382605	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19380031	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19380034	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19380195	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19378110	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19394564	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19393710	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19379311	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19379145	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19393817	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19380040	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19393758	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19394181	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19393660	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19378126	nonsense	probably null
R9737:Vmn2r99	UTSW	17	19362301	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19379301	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- AGTCCTTGAAACTTAGAGGCC -3'
(R):5'- TATGAAGTCCATGTGGCGG -3'

Sequencing Primer
(F):5'- ACTTAGAGGCCTTACAAAATTTTCTC -3'
(R):5'- CGGGGGTCATTTTTACAAAAGC -3'

Posted On

2022-10-06