Mutagenetix > Incidental Mutation

Incidental Mutation 'R9681:Vmn2r99'

728589

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R9681 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19582397-19614852 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19598889 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 191 (Q191L)

Ref Sequence

ENSEMBL: ENSMUSP00000135236 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably damaging
Transcript: ENSMUST00000176107
AA Change: Q191L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: Q191L

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000231989
AA Change: Q191L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610030E20Rik	A	G	6: 72,324,450 (GRCm39)	D20G	probably damaging	Het
Acbd3	T	A	1: 180,566,082 (GRCm39)	Y258*	probably null	Het
Adamts1	T	A	16: 85,599,498 (GRCm39)	H34L		Het
Ankmy1	G	T	1: 92,813,882 (GRCm39)	N432K	possibly damaging	Het
Ano1	A	G	7: 144,143,893 (GRCm39)	M966T	possibly damaging	Het
Ark2n	A	T	18: 77,722,989 (GRCm39)	V349D	possibly damaging	Het
C2cd3	A	G	7: 100,023,662 (GRCm39)	T83A	probably benign	Het
Cdk1	T	A	10: 69,178,449 (GRCm39)	D137V	possibly damaging	Het
Cfap299	T	A	5: 98,477,214 (GRCm39)	M1K	probably null	Het
Chia1	A	G	3: 106,037,996 (GRCm39)	Y326C	probably damaging	Het
Ciz1	A	G	2: 32,260,974 (GRCm39)	D295G	possibly damaging	Het
Clrn1	C	T	3: 58,792,251 (GRCm39)	V71I	probably benign	Het
Cr1l	T	C	1: 194,800,149 (GRCm39)	D175G	probably damaging	Het
Crem	A	T	18: 3,268,067 (GRCm39)	V87E	probably damaging	Het
Cyp2b19	T	A	7: 26,466,328 (GRCm39)	L377Q	probably benign	Het
Ddx42	T	A	11: 106,125,679 (GRCm39)	V243D	probably damaging	Het
Dnah14	T	C	1: 181,562,414 (GRCm39)	V2658A	possibly damaging	Het
Dnah3	T	G	7: 119,677,611 (GRCm39)	M437L	probably benign	Het
Flrt2	T	A	12: 95,745,425 (GRCm39)		probably benign	Het
Gm10142	T	A	10: 77,551,880 (GRCm39)	C80*	probably null	Het
Gpr158	A	G	2: 21,831,315 (GRCm39)	E805G	probably damaging	Het
Grwd1	T	C	7: 45,479,473 (GRCm39)	E134G	probably benign	Het
Hdac11	T	A	6: 91,150,068 (GRCm39)	V289D	probably benign	Het
Hsdl1	T	C	8: 120,293,081 (GRCm39)	E118G	possibly damaging	Het
Igdcc4	T	A	9: 65,041,858 (GRCm39)	L1095Q	possibly damaging	Het
Ildr1	T	A	16: 36,528,749 (GRCm39)	C65S	probably damaging	Het
Itgal	T	C	7: 126,929,422 (GRCm39)	F1113S	probably damaging	Het
Itsn2	G	T	12: 4,683,499 (GRCm39)	V341F	unknown	Het
Jak1	G	A	4: 101,019,085 (GRCm39)	R723C	probably damaging	Het
Limch1	A	G	5: 67,126,422 (GRCm39)	T8A	probably damaging	Het
Limd1	T	A	9: 123,345,903 (GRCm39)	C561S	possibly damaging	Het
Map3k19	A	G	1: 127,750,097 (GRCm39)	F1085L	possibly damaging	Het
Mcm7	A	T	5: 138,164,220 (GRCm39)	Y600*	probably null	Het
Mecom	A	T	3: 30,033,803 (GRCm39)	D300E	probably benign	Het
Mfap4	T	A	11: 61,376,925 (GRCm39)	Y51*	probably null	Het
Mug1	T	A	6: 121,833,254 (GRCm39)	N286K	probably benign	Het
Myl2	A	T	5: 122,240,783 (GRCm39)	R40*	probably null	Het
Nom1	A	G	5: 29,642,623 (GRCm39)	S375G	probably damaging	Het
Nrcam	C	A	12: 44,598,133 (GRCm39)	P368Q	probably null	Het
Oca2	G	C	7: 55,943,623 (GRCm39)	Q265H	probably null	Het
Or52a20	T	A	7: 103,366,475 (GRCm39)	F225I	probably damaging	Het
Or52n2c	T	C	7: 104,574,075 (GRCm39)	T299A	probably damaging	Het
Or5k8	T	A	16: 58,644,176 (GRCm39)	N299Y	possibly damaging	Het
Or6c88	A	T	10: 129,406,664 (GRCm39)	T47S	probably damaging	Het
Or7a41	G	A	10: 78,871,577 (GRCm39)	D316N	probably benign	Het
Palmd	T	C	3: 116,717,120 (GRCm39)	E459G	probably benign	Het
Pde1a	G	T	2: 79,695,465 (GRCm39)	A494D	probably benign	Het
Pds5a	T	C	5: 65,808,587 (GRCm39)	Y428C	probably damaging	Het
Pgm2	T	C	5: 64,254,391 (GRCm39)	F59L	probably benign	Het
Plekhm1	A	G	11: 103,258,950 (GRCm39)	V980A	possibly damaging	Het
Polb	T	C	8: 23,118,346 (GRCm39)	D318G	possibly damaging	Het
Rasgef1c	T	A	11: 49,861,040 (GRCm39)	M335K	probably damaging	Het
Rasgrp4	T	C	7: 28,849,687 (GRCm39)	S651P	probably benign	Het
Robo3	A	C	9: 37,334,558 (GRCm39)	I624S	possibly damaging	Het
Robo3	T	A	9: 37,339,087 (GRCm39)	H290L	probably benign	Het
Rps6kl1	T	C	12: 85,183,599 (GRCm39)	H482R	probably damaging	Het
Slc26a9	A	T	1: 131,681,691 (GRCm39)	E168V	probably benign	Het
Slc3a2	T	C	19: 8,691,226 (GRCm39)		probably benign	Het
Slc4a4	A	T	5: 89,102,723 (GRCm39)	K54*	probably null	Het
Slitrk6	A	G	14: 110,988,258 (GRCm39)	L483P	probably damaging	Het
Svep1	T	A	4: 58,084,959 (GRCm39)	N1793I	probably damaging	Het
Ttn	T	C	2: 76,612,723 (GRCm39)	I17119V	possibly damaging	Het
Tulp4	T	C	17: 6,274,500 (GRCm39)	L617P	possibly damaging	Het
Uhrf1	A	G	17: 56,625,083 (GRCm39)	N542S	possibly damaging	Het
Vmn1r151	T	A	7: 22,198,368 (GRCm39)	T246S	probably damaging	Het
Vmn1r57	T	A	7: 5,224,069 (GRCm39)	V198E	probably damaging	Het
Vrk3	C	T	7: 44,403,356 (GRCm39)	T39M	possibly damaging	Het
Zc3h3	C	A	15: 75,681,470 (GRCm39)	R537L	probably damaging	Het
Zfp64	A	T	2: 168,793,680 (GRCm39)	V22E	probably damaging	Het
Zwint	T	C	10: 72,493,112 (GRCm39)	L218P	probably damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,599,116 (GRCm39)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,614,518 (GRCm39)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,602,885 (GRCm39)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,613,920 (GRCm39)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,600,377 (GRCm39)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,600,494 (GRCm39)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,598,952 (GRCm39)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,598,485 (GRCm39)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,614,547 (GRCm39)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,614,547 (GRCm39)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,614,605 (GRCm39)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,614,835 (GRCm39)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,599,305 (GRCm39)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,582,521 (GRCm39)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,600,322 (GRCm39)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,582,514 (GRCm39)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,598,207 (GRCm39)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,582,415 (GRCm39)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,599,077 (GRCm39)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,598,253 (GRCm39)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,598,891 (GRCm39)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,598,970 (GRCm39)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,614,635 (GRCm39)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,599,252 (GRCm39)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,599,252 (GRCm39)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,598,832 (GRCm39)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,599,522 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,613,924 (GRCm39)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,582,397 (GRCm39)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,598,868 (GRCm39)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,599,601 (GRCm39)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,599,531 (GRCm39)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,614,408 (GRCm39)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,598,210 (GRCm39)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,599,242 (GRCm39)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,602,820 (GRCm39)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,602,820 (GRCm39)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,602,867 (GRCm39)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,600,293 (GRCm39)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,600,296 (GRCm39)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,600,457 (GRCm39)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,598,372 (GRCm39)	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19,614,826 (GRCm39)	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19,613,972 (GRCm39)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,599,573 (GRCm39)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,599,407 (GRCm39)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,614,079 (GRCm39)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,600,302 (GRCm39)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,614,020 (GRCm39)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,614,443 (GRCm39)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,613,922 (GRCm39)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,598,388 (GRCm39)	nonsense	probably null
R9737:Vmn2r99	UTSW	17	19,582,563 (GRCm39)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,599,563 (GRCm39)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- AGTCCTTGAAACTTAGAGGCC -3'
(R):5'- TATGAAGTCCATGTGGCGG -3'

Sequencing Primer
(F):5'- ACTTAGAGGCCTTACAAAATTTTCTC -3'
(R):5'- CGGGGGTCATTTTTACAAAAGC -3'

Posted On

2022-10-06