Incidental Mutation 'R9683:Gpr155'
| ID |
728666 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr155
|
| Ensembl Gene |
ENSMUSG00000041762 |
| Gene Name |
G protein-coupled receptor 155 |
| Synonyms |
DEPDC3, 1110017O10Rik, PGR22, F730029F15Rik |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R9683 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
73171850-73216842 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 73192780 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Threonine
at position 585
(I585T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000075788
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000076463]
[ENSMUST00000112043]
[ENSMUST00000112044]
|
| AlphaFold |
A2AWR3 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000076463
AA Change: I585T
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: I585T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112043
AA Change: I585T
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: I585T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000112044
AA Change: I557T
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: I557T
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
8.3e-11 |
PFAM |
|
transmembrane domain
|
382 |
404 |
N/A |
INTRINSIC |
|
transmembrane domain
|
414 |
436 |
N/A |
INTRINSIC |
|
transmembrane domain
|
449 |
471 |
N/A |
INTRINSIC |
|
transmembrane domain
|
491 |
510 |
N/A |
INTRINSIC |
|
transmembrane domain
|
629 |
651 |
N/A |
INTRINSIC |
|
transmembrane domain
|
661 |
683 |
N/A |
INTRINSIC |
|
DEP
|
731 |
805 |
8.28e-12 |
SMART |
|
| Predicted Effect |
|
| SMART Domains |
Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
AA Change: I25T
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
98 |
120 |
N/A |
INTRINSIC |
|
transmembrane domain
|
130 |
152 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.7%
- 20x: 99.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 60 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700006A11Rik |
C |
T |
3: 124,200,095 (GRCm39) |
G499D |
probably benign |
Het |
| Acr |
T |
A |
15: 89,457,440 (GRCm39) |
Y229* |
probably null |
Het |
| Adam6b |
G |
T |
12: 113,454,176 (GRCm39) |
C331F |
probably benign |
Het |
| Ahnak |
T |
C |
19: 8,984,719 (GRCm39) |
V2001A |
possibly damaging |
Het |
| Ankrd11 |
C |
T |
8: 123,617,682 (GRCm39) |
A2057T |
probably benign |
Het |
| Anln |
A |
T |
9: 22,283,536 (GRCm39) |
C432* |
probably null |
Het |
| Aox4 |
T |
C |
1: 58,278,462 (GRCm39) |
|
probably null |
Het |
| Astn1 |
A |
G |
1: 158,491,619 (GRCm39) |
I1007V |
possibly damaging |
Het |
| Bicral |
T |
C |
17: 47,122,944 (GRCm39) |
D649G |
possibly damaging |
Het |
| C1qbp |
G |
A |
11: 70,873,749 (GRCm39) |
R10C |
probably damaging |
Het |
| Cass4 |
C |
T |
2: 172,268,656 (GRCm39) |
P246L |
probably damaging |
Het |
| Celsr3 |
T |
A |
9: 108,704,522 (GRCm39) |
V335E |
probably damaging |
Het |
| Cfap52 |
T |
A |
11: 67,822,639 (GRCm39) |
T411S |
probably benign |
Het |
| Cnot6 |
A |
T |
11: 49,580,164 (GRCm39) |
L43I |
possibly damaging |
Het |
| Col9a3 |
G |
A |
2: 180,248,322 (GRCm39) |
|
probably null |
Het |
| Dnah14 |
A |
G |
1: 181,426,509 (GRCm39) |
R210G |
probably benign |
Het |
| Fat4 |
A |
G |
3: 38,943,332 (GRCm39) |
S742G |
possibly damaging |
Het |
| Fbn2 |
C |
T |
18: 58,186,099 (GRCm39) |
V1750M |
probably benign |
Het |
| Glrx2 |
A |
G |
1: 143,622,292 (GRCm39) |
D96G |
probably damaging |
Het |
| Gm8369 |
T |
C |
19: 11,489,097 (GRCm39) |
L128P |
probably damaging |
Het |
| Heatr1 |
T |
C |
13: 12,449,140 (GRCm39) |
S1907P |
probably damaging |
Het |
| Hscb |
T |
C |
5: 110,983,881 (GRCm39) |
T129A |
possibly damaging |
Het |
| Itgb5 |
T |
A |
16: 33,740,335 (GRCm39) |
Y482N |
probably damaging |
Het |
| Kmt5b |
T |
A |
19: 3,865,587 (GRCm39) |
*884R |
probably null |
Het |
| L3mbtl1 |
A |
T |
2: 162,812,228 (GRCm39) |
T758S |
possibly damaging |
Het |
| Mindy1 |
A |
T |
3: 95,202,176 (GRCm39) |
H351L |
probably benign |
Het |
| Mmel1 |
A |
T |
4: 154,977,285 (GRCm39) |
I552F |
probably damaging |
Het |
| Mos |
T |
C |
4: 3,871,186 (GRCm39) |
D210G |
probably benign |
Het |
| Npl |
A |
C |
1: 153,421,030 (GRCm39) |
I16S |
possibly damaging |
Het |
| Nutm2 |
C |
A |
13: 50,629,017 (GRCm39) |
P694T |
possibly damaging |
Het |
| Or1e1c |
G |
A |
11: 73,265,811 (GRCm39) |
V82I |
probably damaging |
Het |
| Or52ae7 |
A |
G |
7: 103,119,157 (GRCm39) |
|
probably benign |
Het |
| Plekhg4 |
T |
C |
8: 106,102,923 (GRCm39) |
F261L |
probably benign |
Het |
| Ppfia3 |
T |
A |
7: 45,005,999 (GRCm39) |
N331I |
probably benign |
Het |
| Ppp1r12a |
T |
A |
10: 108,096,747 (GRCm39) |
S712R |
possibly damaging |
Het |
| Psg20 |
T |
A |
7: 18,416,508 (GRCm39) |
K203* |
probably null |
Het |
| Recql |
C |
T |
6: 142,305,646 (GRCm39) |
R234Q |
|
Het |
| Rerg |
A |
G |
6: 137,033,252 (GRCm39) |
F160S |
probably damaging |
Het |
| Rp1l1 |
A |
G |
14: 64,269,126 (GRCm39) |
K1571E |
probably damaging |
Het |
| Rsrc1 |
T |
A |
3: 67,257,328 (GRCm39) |
S247T |
probably damaging |
Het |
| Sacs |
T |
A |
14: 61,450,881 (GRCm39) |
I4309N |
possibly damaging |
Het |
| Setd7 |
A |
T |
3: 51,450,111 (GRCm39) |
I105N |
possibly damaging |
Het |
| Sgk1 |
AAGA |
AAGAGA |
10: 21,868,290 (GRCm39) |
|
probably null |
Het |
| Siglec1 |
T |
A |
2: 130,921,236 (GRCm39) |
H645L |
probably damaging |
Het |
| Slain1 |
A |
T |
14: 103,925,621 (GRCm39) |
D323V |
probably damaging |
Het |
| Slc25a10 |
A |
G |
11: 120,386,312 (GRCm39) |
N139D |
probably damaging |
Het |
| Slc9a9 |
T |
G |
9: 94,552,235 (GRCm39) |
F41V |
probably damaging |
Het |
| Spag9 |
A |
C |
11: 93,988,568 (GRCm39) |
E879D |
probably damaging |
Het |
| Tdrd7 |
T |
A |
4: 46,025,946 (GRCm39) |
L922Q |
probably damaging |
Het |
| Tiprl |
A |
G |
1: 165,050,147 (GRCm39) |
F156S |
probably damaging |
Het |
| Tmem185b |
G |
C |
1: 119,454,748 (GRCm39) |
V170L |
probably damaging |
Het |
| Top2a |
G |
A |
11: 98,887,683 (GRCm39) |
T1275I |
probably benign |
Het |
| Tpp1 |
A |
G |
7: 105,398,104 (GRCm39) |
L353P |
probably damaging |
Het |
| Twnk |
T |
A |
19: 44,998,622 (GRCm39) |
H513Q |
probably damaging |
Het |
| Ubr5 |
C |
T |
15: 37,978,271 (GRCm39) |
V2467I |
|
Het |
| Vmn2r16 |
T |
A |
5: 109,511,677 (GRCm39) |
I628N |
probably damaging |
Het |
| Zan |
T |
C |
5: 137,462,776 (GRCm39) |
E801G |
unknown |
Het |
| Zfp810 |
T |
C |
9: 22,189,799 (GRCm39) |
R370G |
possibly damaging |
Het |
| Zfp955a |
A |
G |
17: 33,461,587 (GRCm39) |
S182P |
probably benign |
Het |
| Znrf3 |
T |
C |
11: 5,394,465 (GRCm39) |
T72A |
possibly damaging |
Het |
|
| Other mutations in Gpr155 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00731:Gpr155
|
APN |
2 |
73,192,957 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01432:Gpr155
|
APN |
2 |
73,182,229 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
IGL01528:Gpr155
|
APN |
2 |
73,192,767 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01718:Gpr155
|
APN |
2 |
73,212,576 (GRCm39) |
missense |
probably benign |
|
|
IGL01733:Gpr155
|
APN |
2 |
73,183,956 (GRCm39) |
splice site |
probably null |
|
|
IGL03342:Gpr155
|
APN |
2 |
73,180,022 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT1430001:Gpr155
|
UTSW |
2 |
73,200,482 (GRCm39) |
missense |
probably benign |
0.08 |
|
PIT4810001:Gpr155
|
UTSW |
2 |
73,178,607 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0226:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0399:Gpr155
|
UTSW |
2 |
73,200,346 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R0445:Gpr155
|
UTSW |
2 |
73,200,488 (GRCm39) |
splice site |
probably benign |
|
|
R1570:Gpr155
|
UTSW |
2 |
73,200,382 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1598:Gpr155
|
UTSW |
2 |
73,200,434 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1647:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1648:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1756:Gpr155
|
UTSW |
2 |
73,197,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1760:Gpr155
|
UTSW |
2 |
73,212,279 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2033:Gpr155
|
UTSW |
2 |
73,178,526 (GRCm39) |
missense |
probably benign |
0.18 |
|
R2044:Gpr155
|
UTSW |
2 |
73,203,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2145:Gpr155
|
UTSW |
2 |
73,187,002 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2172:Gpr155
|
UTSW |
2 |
73,212,471 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2274:Gpr155
|
UTSW |
2 |
73,178,479 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3878:Gpr155
|
UTSW |
2 |
73,198,736 (GRCm39) |
nonsense |
probably null |
|
|
R3924:Gpr155
|
UTSW |
2 |
73,200,420 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Gpr155
|
UTSW |
2 |
73,197,882 (GRCm39) |
nonsense |
probably null |
|
|
R4950:Gpr155
|
UTSW |
2 |
73,212,529 (GRCm39) |
missense |
probably benign |
|
|
R5337:Gpr155
|
UTSW |
2 |
73,178,592 (GRCm39) |
missense |
probably benign |
0.32 |
|
R5830:Gpr155
|
UTSW |
2 |
73,200,433 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R5887:Gpr155
|
UTSW |
2 |
73,174,062 (GRCm39) |
nonsense |
probably null |
|
|
R5929:Gpr155
|
UTSW |
2 |
73,204,011 (GRCm39) |
nonsense |
probably null |
|
|
R6293:Gpr155
|
UTSW |
2 |
73,204,341 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6553:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6585:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7003:Gpr155
|
UTSW |
2 |
73,173,961 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7353:Gpr155
|
UTSW |
2 |
73,197,835 (GRCm39) |
nonsense |
probably null |
|
|
R7506:Gpr155
|
UTSW |
2 |
73,198,683 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7631:Gpr155
|
UTSW |
2 |
73,213,291 (GRCm39) |
intron |
probably benign |
|
|
R7753:Gpr155
|
UTSW |
2 |
73,212,550 (GRCm39) |
missense |
probably benign |
0.27 |
|
R7810:Gpr155
|
UTSW |
2 |
73,212,296 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7813:Gpr155
|
UTSW |
2 |
73,212,329 (GRCm39) |
nonsense |
probably null |
|
|
R7815:Gpr155
|
UTSW |
2 |
73,192,904 (GRCm39) |
missense |
probably benign |
|
|
R7873:Gpr155
|
UTSW |
2 |
73,173,934 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R8506:Gpr155
|
UTSW |
2 |
73,173,806 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8680:Gpr155
|
UTSW |
2 |
73,174,039 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8856:Gpr155
|
UTSW |
2 |
73,203,993 (GRCm39) |
missense |
probably benign |
0.27 |
|
R8872:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9116:Gpr155
|
UTSW |
2 |
73,204,109 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
| Predicted Primers |
PCR Primer
(F):5'- CAGCTCAATGAATGAAGTGCTAG -3'
(R):5'- GTTTTGATGCTGACAGATGATCACC -3'
Sequencing Primer
(F):5'- TGAAGTGCTAGAAAAATGAAGCTC -3'
(R):5'- GATGATCACCACGGCAGTC -3'
|
| Posted On |
2022-10-06 |
Incidental Mutation