Mutagenetix > Incidental Mutation

Incidental Mutation 'R9683:Gpr155'

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Institutional Source

Beutler Lab

Gene Symbol

Gpr155

Ensembl Gene

ENSMUSG00000041762

Gene Name

G protein-coupled receptor 155

Synonyms

PGR22, 1110017O10Rik, DEPDC3, F730029F15Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9683 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73341506-73386572 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 73362436 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 585 (I585T)

Ref Sequence

ENSEMBL: ENSMUSP00000075788 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076463] [ENSMUST00000112043] [ENSMUST00000112044]

AlphaFold

A2AWR3

Predicted Effect

probably benign
Transcript: ENSMUST00000076463
AA Change: I585T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: I585T

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112043
AA Change: I585T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: I585T

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112044
AA Change: I557T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: I557T

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
Pfam:Mem_trans	187	360	8.3e-11	PFAM
transmembrane domain	382	404	N/A	INTRINSIC
transmembrane domain	414	436	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
transmembrane domain	491	510	N/A	INTRINSIC
transmembrane domain	629	651	N/A	INTRINSIC
transmembrane domain	661	683	N/A	INTRINSIC
DEP	731	805	8.28e-12	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
AA Change: I25T

Domain	Start	End	E-Value	Type
transmembrane domain	98	120	N/A	INTRINSIC
transmembrane domain	130	152	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	C	T	3: 124,406,446	G499D	probably benign	Het
Acr	T	A	15: 89,573,237	Y229*	probably null	Het
Adam6b	G	T	12: 113,490,556	C331F	probably benign	Het
Ahnak	T	C	19: 9,007,355	V2001A	possibly damaging	Het
Ankrd11	C	T	8: 122,890,943	A2057T	probably benign	Het
Anln	A	T	9: 22,372,240	C432*	probably null	Het
Aox4	T	C	1: 58,239,303		probably null	Het
Astn1	A	G	1: 158,664,049	I1007V	possibly damaging	Het
Bicral	T	C	17: 46,812,018	D649G	possibly damaging	Het
C1qbp	G	A	11: 70,982,923	R10C	probably damaging	Het
Cass4	C	T	2: 172,426,736	P246L	probably damaging	Het
Celsr3	T	A	9: 108,827,323	V335E	probably damaging	Het
Cfap52	T	A	11: 67,931,813	T411S	probably benign	Het
Cnot6	A	T	11: 49,689,337	L43I	possibly damaging	Het
Col9a3	G	A	2: 180,606,529		probably null	Het
Dnah14	A	G	1: 181,598,944	R210G	probably benign	Het
Fat4	A	G	3: 38,889,183	S742G	possibly damaging	Het
Fbn2	C	T	18: 58,053,027	V1750M	probably benign	Het
Glrx2	A	G	1: 143,746,554	D96G	probably damaging	Het
Gm8369	T	C	19: 11,511,733	L128P	probably damaging	Het
Heatr1	T	C	13: 12,434,259	S1907P	probably damaging	Het
Hscb	T	C	5: 110,836,015	T129A	possibly damaging	Het
Itgb5	T	A	16: 33,919,965	Y482N	probably damaging	Het
Kmt5b	T	A	19: 3,815,587	*884R	probably null	Het
L3mbtl1	A	T	2: 162,970,308	T758S	possibly damaging	Het
Mindy1	A	T	3: 95,294,865	H351L	probably benign	Het
Mmel1	A	T	4: 154,892,828	I552F	probably damaging	Het
Mos	T	C	4: 3,871,186	D210G	probably benign	Het
Npl	A	C	1: 153,545,284	I16S	possibly damaging	Het
Nutm2	C	A	13: 50,474,981	P694T	possibly damaging	Het
Olfr376	G	A	11: 73,374,985	V82I	probably damaging	Het
Olfr608	A	G	7: 103,469,950		probably benign	Het
Plekhg4	T	C	8: 105,376,291	F261L	probably benign	Het
Ppfia3	T	A	7: 45,356,575	N331I	probably benign	Het
Ppp1r12a	T	A	10: 108,260,886	S712R	possibly damaging	Het
Psg20	T	A	7: 18,682,583	K203*	probably null	Het
Recql	C	T	6: 142,359,920	R234Q		Het
Rerg	A	G	6: 137,056,254	F160S	probably damaging	Het
Rp1l1	A	G	14: 64,031,677	K1571E	probably damaging	Het
Rsrc1	T	A	3: 67,349,995	S247T	probably damaging	Het
Sacs	T	A	14: 61,213,432	I4309N	possibly damaging	Het
Setd7	A	T	3: 51,542,690	I105N	possibly damaging	Het
Sgk1	AAGA	AAGAGA	10: 21,992,391		probably null	Het
Siglec1	T	A	2: 131,079,316	H645L	probably damaging	Het
Slain1	A	T	14: 103,688,185	D323V	probably damaging	Het
Slc25a10	A	G	11: 120,495,486	N139D	probably damaging	Het
Slc9a9	T	G	9: 94,670,182	F41V	probably damaging	Het
Spag9	A	C	11: 94,097,742	E879D	probably damaging	Het
Tdrd7	T	A	4: 46,025,946	L922Q	probably damaging	Het
Tiprl	A	G	1: 165,222,578	F156S	probably damaging	Het
Tmem185b	G	C	1: 119,527,018	V170L	probably damaging	Het
Top2a	G	A	11: 98,996,857	T1275I	probably benign	Het
Tpp1	A	G	7: 105,748,897	L353P	probably damaging	Het
Twnk	T	A	19: 45,010,183	H513Q	probably damaging	Het
Ubr5	C	T	15: 37,978,027	V2467I		Het
Vmn2r16	T	A	5: 109,363,811	I628N	probably damaging	Het
Zan	T	C	5: 137,464,514	E801G	unknown	Het
Zfp810	T	C	9: 22,278,503	R370G	possibly damaging	Het
Zfp955a	A	G	17: 33,242,613	S182P	probably benign	Het
Znrf3	T	C	11: 5,444,465	T72A	possibly damaging	Het

Other mutations in Gpr155

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00731:Gpr155	APN	2	73362613	missense	probably damaging	1.00
IGL01432:Gpr155	APN	2	73351885	missense	possibly damaging	0.51
IGL01528:Gpr155	APN	2	73362423	critical splice donor site	probably null
IGL01718:Gpr155	APN	2	73382232	missense	probably benign
IGL01733:Gpr155	APN	2	73353612	splice site	probably null
IGL03342:Gpr155	APN	2	73349678	missense	probably damaging	1.00
PIT1430001:Gpr155	UTSW	2	73370138	missense	probably benign	0.08
PIT4810001:Gpr155	UTSW	2	73348263	missense	probably benign	0.08
R0226:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R0399:Gpr155	UTSW	2	73370002	missense	possibly damaging	0.91
R0445:Gpr155	UTSW	2	73370144	splice site	probably benign
R1570:Gpr155	UTSW	2	73370038	missense	possibly damaging	0.87
R1598:Gpr155	UTSW	2	73370090	missense	probably damaging	0.98
R1647:Gpr155	UTSW	2	73364164	splice site	probably null
R1648:Gpr155	UTSW	2	73364164	splice site	probably null
R1756:Gpr155	UTSW	2	73367577	missense	probably benign	0.00
R1760:Gpr155	UTSW	2	73381935	missense	probably damaging	1.00
R2033:Gpr155	UTSW	2	73348182	missense	probably benign	0.18
R2044:Gpr155	UTSW	2	73373633	missense	probably damaging	1.00
R2145:Gpr155	UTSW	2	73356658	missense	probably benign	0.01
R2172:Gpr155	UTSW	2	73382127	missense	probably benign	0.00
R2274:Gpr155	UTSW	2	73348135	critical splice donor site	probably null
R3878:Gpr155	UTSW	2	73368392	nonsense	probably null
R3924:Gpr155	UTSW	2	73370076	missense	probably damaging	1.00
R4910:Gpr155	UTSW	2	73367538	nonsense	probably null
R4950:Gpr155	UTSW	2	73382185	missense	probably benign
R5337:Gpr155	UTSW	2	73348248	missense	probably benign	0.32
R5830:Gpr155	UTSW	2	73370089	missense	possibly damaging	0.93
R5887:Gpr155	UTSW	2	73343718	nonsense	probably null
R5929:Gpr155	UTSW	2	73373667	nonsense	probably null
R6293:Gpr155	UTSW	2	73373997	missense	possibly damaging	0.47
R6553:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R6585:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R7003:Gpr155	UTSW	2	73343617	missense	probably damaging	0.99
R7353:Gpr155	UTSW	2	73367491	nonsense	probably null
R7506:Gpr155	UTSW	2	73368339	missense	probably damaging	0.97
R7631:Gpr155	UTSW	2	73382947	intron	probably benign
R7753:Gpr155	UTSW	2	73382206	missense	probably benign	0.27
R7810:Gpr155	UTSW	2	73381952	missense	probably damaging	0.99
R7813:Gpr155	UTSW	2	73381985	nonsense	probably null
R7815:Gpr155	UTSW	2	73362560	missense	probably benign
R7873:Gpr155	UTSW	2	73343590	missense	possibly damaging	0.51
R8506:Gpr155	UTSW	2	73343462	missense	probably damaging	0.99
R8680:Gpr155	UTSW	2	73343695	missense	probably damaging	0.99
R8856:Gpr155	UTSW	2	73373649	missense	probably benign	0.27
R8872:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R9116:Gpr155	UTSW	2	73373765	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- CAGCTCAATGAATGAAGTGCTAG -3'
(R):5'- GTTTTGATGCTGACAGATGATCACC -3'

Sequencing Primer
(F):5'- TGAAGTGCTAGAAAAATGAAGCTC -3'
(R):5'- GATGATCACCACGGCAGTC -3'

Posted On

2022-10-06