Mutagenetix > Incidental Mutation

Incidental Mutation 'R9685:Vmn2r6'

728781

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG620718, EG667069

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.179) question?

Stock #

R9685 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64537561-64565298 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 64556660 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 251 (V251A)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

probably benign
Transcript: ENSMUST00000165012
AA Change: V162A

PolyPhen 2 Score 0.298 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: V162A

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000176481
AA Change: V251A

PolyPhen 2 Score 0.188 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: V251A

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930453N24Rik	G	T	16: 64,766,460	N300K	possibly damaging	Het
A130010J15Rik	G	A	1: 193,174,757	R139H	probably benign	Het
Aanat	A	C	11: 116,596,855	T127P	possibly damaging	Het
Acp4	G	A	7: 44,257,309		probably benign	Het
Arhgef5	T	G	6: 43,273,593	I426S	probably benign	Het
Bcl2a1a	T	C	9: 88,957,132	S28P	probably benign	Het
Cd163	T	C	6: 124,311,425	S272P	possibly damaging	Het
Cdk13	C	A	13: 17,803,957	R232L	unknown	Het
Clrn2	A	G	5: 45,453,989	D60G	possibly damaging	Het
Cnot6l	A	G	5: 96,082,890	L406P	probably damaging	Het
Cspg4	T	G	9: 56,890,338	V1362G	probably benign	Het
Cspp1	C	T	1: 10,126,414	S939L	probably benign	Het
Dcaf1	C	T	9: 106,836,619	T145I	probably benign	Het
Ddx28	T	C	8: 106,010,101	S442G	probably benign	Het
Dnajc3	A	T	14: 118,972,363	R283S	probably benign	Het
Dsc1	G	A	18: 20,099,030	T307I	possibly damaging	Het
Elovl5	T	C	9: 77,961,009	Y68H	probably damaging	Het
Fam25c	G	A	14: 34,355,309	T17I	probably damaging	Het
Fryl	T	C	5: 73,059,536	E2137G	probably damaging	Het
Gm10142	A	G	10: 77,715,928	Q41R	unknown	Het
Gm21671	AACT	A	5: 25,950,851		probably benign	Het
Grm1	T	C	10: 10,689,031	T1178A	possibly damaging	Het
Hhip	A	T	8: 79,996,734	H430Q	probably damaging	Het
Hip1	A	G	5: 135,449,822	F178L	probably damaging	Het
Impa1	T	A	3: 10,328,370	I58L	probably benign	Het
Irak4	T	A	15: 94,553,931	V135E	probably benign	Het
Jup	A	C	11: 100,383,411	L151R	probably damaging	Het
Lrrn4cl	T	A	19: 8,852,055	N132K	probably damaging	Het
Mapkbp1	C	A	2: 120,021,183	R863S	probably benign	Het
Olfr1008	T	C	2: 85,689,522	F31S	probably damaging	Het
Pramef17	T	A	4: 143,992,950	M282L	probably benign	Het
Rasa4	A	G	5: 136,095,529	D144G	probably benign	Het
Rrs1	T	A	1: 9,546,165	S214R	probably benign	Het
Rsf1	CGGC	CGGCGGCGGGGGC	7: 97,579,932		probably benign	Het
Rtca	T	A	3: 116,499,576	R195S	probably benign	Het
Scaper	A	T	9: 55,864,551	S360R	probably benign	Het
Scarb1	A	T	5: 125,294,130	F293I	possibly damaging	Het
Snapc1	T	C	12: 73,970,341		probably null	Het
Spta1	T	C	1: 174,205,359	V994A	probably damaging	Het
Synpo2	T	C	3: 123,117,717	E93G	probably damaging	Het
Ubr4	A	G	4: 139,464,030	T985A	unknown	Het
Vmn1r18	T	C	6: 57,390,478	I30M	possibly damaging	Het
Vmn2r15	A	G	5: 109,292,732	V420A	probably benign	Het
Zfyve16	A	T	13: 92,522,803	I200N	possibly damaging	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64538104	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64556345	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64537902	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64556189	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64556328	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64556490	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64556496	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64565153	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64538007	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64559803	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64559803	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64559545	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64539912	missense	probably benign
R0206:Vmn2r6	UTSW	3	64539912	missense	probably benign
R0208:Vmn2r6	UTSW	3	64539912	missense	probably benign
R0427:Vmn2r6	UTSW	3	64559587	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64556302	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64556840	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64538066	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64565067	missense	probably benign	0.01
R1245:Vmn2r6	UTSW	3	64556790	missense	possibly damaging	0.53
R1295:Vmn2r6	UTSW	3	64538273	missense	probably damaging	1.00
R1473:Vmn2r6	UTSW	3	64538158	nonsense	probably null
R1498:Vmn2r6	UTSW	3	64556469	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64556277	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64537841	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64556098	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64559718	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64556669	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64556669	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64556352	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64538286	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64556790	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64547339	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64547339	missense	probably benign	0.00
R3766:Vmn2r6	UTSW	3	64556508	missense	probably benign	0.19
R3870:Vmn2r6	UTSW	3	64556621	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64556472	missense	probably benign	0.05
R4024:Vmn2r6	UTSW	3	64538250	missense	possibly damaging	0.73
R4026:Vmn2r6	UTSW	3	64538250	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64537948	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64537724	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64559647	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64547408	missense	probably benign
R4934:Vmn2r6	UTSW	3	64556345	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64537786	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64537623	missense	possibly damaging	0.89
R5148:Vmn2r6	UTSW	3	64556594	missense	probably damaging	0.99
R5155:Vmn2r6	UTSW	3	64538514	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64537990	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64556842	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64556033	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64565231	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64556532	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64559755	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64538003	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64556805	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64547380	nonsense	probably null
R6645:Vmn2r6	UTSW	3	64556876	missense	probably damaging	1.00
R6791:Vmn2r6	UTSW	3	64538159	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64556774	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64539951	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64556520	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64565262	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64565142	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64556570	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64538022	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64559803	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64559820	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64559824	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64547643	intron	probably benign
R8169:Vmn2r6	UTSW	3	64539889	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64556105	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64559800	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64556155	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64556856	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64559611	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64556063	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64547250	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64538303	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64556128	missense	possibly damaging	0.90
X0020:Vmn2r6	UTSW	3	64538450	missense	probably benign
X0066:Vmn2r6	UTSW	3	64547378	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64556325	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GTGGCTATCCATGTCCTGTC -3'
(R):5'- TCCAGGTGGGATATGCTTCTTCC -3'

Sequencing Primer
(F):5'- GGCTATCCATGTCCTGTCAGTTATG -3'
(R):5'- GGTGGGATATGCTTCTTCCTCCTC -3'

Posted On

2022-10-06