Mutagenetix > Incidental Mutation

Incidental Mutation 'R9686:Or5g27'

728828

Institutional Source

Beutler Lab

Gene Symbol

Or5g27

Ensembl Gene

ENSMUSG00000111179

Gene Name

olfactory receptor family 5 subfamily G member 27

Synonyms

MOR175-4, GA_x6K02T2Q125-47058060-47059004, Olfr996

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R9686 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

85409559-85410626 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 85410340 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 252 (Y252*)

Ref Sequence

ENSEMBL: ENSMUSP00000076330 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077075]

AlphaFold

Q7TRA0

Predicted Effect

probably null
Transcript: ENSMUST00000077075
AA Change: Y252*

SMART Domains

Protein: ENSMUSP00000076330
Gene: ENSMUSG00000111179
AA Change: Y252*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.2e-52	PFAM
Pfam:7tm_1	41	290	2.7e-16	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc3	A	G	11: 94,247,867 (GRCm39)	F1209L	probably benign	Het
Adamts19	A	G	18: 58,971,093 (GRCm39)	T226A	probably benign	Het
Akap13	A	G	7: 75,236,084 (GRCm39)	T220A	probably damaging	Het
Ank2	T	A	3: 126,740,550 (GRCm39)	E1778V	unknown	Het
Aspn	T	C	13: 49,710,829 (GRCm39)	I158T	probably damaging	Het
Btbd1	A	G	7: 81,455,598 (GRCm39)	C258R	probably damaging	Het
Btla	A	G	16: 45,070,872 (GRCm39)	N278S	probably damaging	Het
Cap2	A	G	13: 46,678,926 (GRCm39)	E12G	probably damaging	Het
Ccdc171	A	G	4: 83,467,919 (GRCm39)	Y88C	probably damaging	Het
Ceacam3	C	T	7: 16,892,123 (GRCm39)	L289F		Het
Cenpj	G	A	14: 56,790,048 (GRCm39)	A667V	probably benign	Het
Clu	G	T	14: 66,212,454 (GRCm39)	G136C	probably damaging	Het
Col4a4	A	T	1: 82,474,962 (GRCm39)	F626I	unknown	Het
Csf2ra	A	G	19: 61,213,629 (GRCm39)	F360S	probably damaging	Het
Cyp27b1	T	A	10: 126,886,185 (GRCm39)	S289T	probably benign	Het
Dnah17	G	T	11: 117,979,048 (GRCm39)	H1781Q	possibly damaging	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Fap	T	C	2: 62,403,857 (GRCm39)	K6R	possibly damaging	Het
Fcer1a	A	G	1: 173,052,980 (GRCm39)	V72A	possibly damaging	Het
Flnc	T	C	6: 29,456,434 (GRCm39)	S2211P	possibly damaging	Het
Gm20939	A	T	17: 95,184,888 (GRCm39)	H512L	probably damaging	Het
Gng12	T	C	6: 66,994,529 (GRCm39)	S59P	probably damaging	Het
Grin1	A	C	2: 25,203,522 (GRCm39)	I163S	probably benign	Het
Haus5	A	G	7: 30,361,398 (GRCm39)	I74T	probably benign	Het
Hmcn1	G	A	1: 150,613,356 (GRCm39)	P1357L	probably damaging	Het
Kcnv1	A	G	15: 44,972,500 (GRCm39)	I461T	probably benign	Het
Kmo	A	G	1: 175,459,786 (GRCm39)	M1V	probably null	Het
Krt80	G	A	15: 101,262,281 (GRCm39)	R107C	probably damaging	Het
Macf1	A	G	4: 123,377,491 (GRCm39)	V1481A	possibly damaging	Het
Masp1	A	G	16: 23,314,887 (GRCm39)	S125P	probably damaging	Het
Mroh2b	C	T	15: 4,974,605 (GRCm39)	P1195L	probably benign	Het
Neurl3	A	T	1: 36,305,539 (GRCm39)	W223R	probably benign	Het
Or10h28	A	G	17: 33,487,853 (GRCm39)	T52A	probably benign	Het
Or5k1b	T	C	16: 58,581,126 (GRCm39)	K138E	possibly damaging	Het
Or5m9	A	T	2: 85,877,335 (GRCm39)	I170F	probably benign	Het
Pcdhb4	A	C	18: 37,442,943 (GRCm39)	Y751S	probably damaging	Het
Pcnx2	C	A	8: 126,592,766 (GRCm39)	A733S	probably benign	Het
Pde4a	A	G	9: 21,117,562 (GRCm39)	E608G	probably benign	Het
Pfdn2	G	A	1: 171,185,377 (GRCm39)	E98K	unknown	Het
Pikfyve	T	A	1: 65,291,615 (GRCm39)	V1278D	probably damaging	Het
Ptprb	A	T	10: 116,204,694 (GRCm39)	T1843S	probably damaging	Het
Rab12	G	T	17: 66,826,513 (GRCm39)	A66E	probably benign	Het
Ralyl	T	C	3: 13,841,887 (GRCm39)		probably benign	Het
Rbm47	A	T	5: 66,179,969 (GRCm39)		probably null	Het
Rev3l	T	A	10: 39,743,384 (GRCm39)	D3038E	possibly damaging	Het
Rhobtb2	A	G	14: 70,034,005 (GRCm39)	F407L	probably benign	Het
S100pbp	A	T	4: 129,049,271 (GRCm39)	D332E	probably damaging	Het
Slc9c1	T	C	16: 45,400,577 (GRCm39)	V729A	possibly damaging	Het
Slfn5	A	T	11: 82,848,001 (GRCm39)	E295D	probably benign	Het
Smarcc2	A	G	10: 128,316,775 (GRCm39)	S518G	probably damaging	Het
Sorbs1	T	C	19: 40,381,954 (GRCm39)	D29G	probably damaging	Het
Spata13	G	A	14: 60,989,108 (GRCm39)	E925K	probably damaging	Het
Spen	A	G	4: 141,199,946 (GRCm39)	S2894P	probably benign	Het
Taar7a	G	A	10: 23,869,309 (GRCm39)	T24I	probably benign	Het
Taf4b	C	A	18: 14,932,215 (GRCm39)	T189N	possibly damaging	Het
Tle7	T	A	8: 110,836,563 (GRCm39)	W180R	probably damaging	Het
Ube2o	A	T	11: 116,434,779 (GRCm39)	C561S	probably benign	Het
Usp34	T	A	11: 23,424,351 (GRCm39)	I3213N		Het
Vmn1r103	A	G	7: 20,244,353 (GRCm39)	V36A	possibly damaging	Het
Vmn2r65	G	A	7: 84,590,084 (GRCm39)	L611F	probably benign	Het
Zfp51	C	T	17: 21,683,871 (GRCm39)	S162F	probably damaging	Het
Zfp518b	G	A	5: 38,831,457 (GRCm39)	H183Y	probably damaging	Het
Zfp622	A	G	15: 25,985,141 (GRCm39)	E169G	probably benign	Het
Zfp703	A	G	8: 27,469,044 (GRCm39)	D236G	probably damaging	Het
Zfp839	A	T	12: 110,821,932 (GRCm39)	I249F	probably damaging	Het

Other mutations in Or5g27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01550:Or5g27	APN	2	85,409,875 (GRCm39)	nonsense	probably null
IGL01972:Or5g27	APN	2	85,410,117 (GRCm39)	missense	probably damaging	1.00
IGL02102:Or5g27	APN	2	85,410,017 (GRCm39)	missense	probably damaging	0.98
IGL03159:Or5g27	APN	2	85,410,284 (GRCm39)	missense	probably damaging	0.99
R0539:Or5g27	UTSW	2	85,410,119 (GRCm39)	missense	probably damaging	0.99
R4275:Or5g27	UTSW	2	85,410,207 (GRCm39)	missense	probably benign	0.07
R4561:Or5g27	UTSW	2	85,409,964 (GRCm39)	missense	probably damaging	0.99
R4953:Or5g27	UTSW	2	85,410,069 (GRCm39)	nonsense	probably null
R5794:Or5g27	UTSW	2	85,409,685 (GRCm39)	missense	probably benign	0.03
R6061:Or5g27	UTSW	2	85,409,886 (GRCm39)	missense	possibly damaging	0.48
R6695:Or5g27	UTSW	2	85,409,793 (GRCm39)	missense	probably damaging	1.00
R6981:Or5g27	UTSW	2	85,409,825 (GRCm39)	missense	probably benign	0.06
R7030:Or5g27	UTSW	2	85,409,746 (GRCm39)	missense	possibly damaging	0.56
R7399:Or5g27	UTSW	2	85,409,640 (GRCm39)	missense	probably benign	0.00
R8914:Or5g27	UTSW	2	85,410,056 (GRCm39)	missense	possibly damaging	0.72
R9594:Or5g27	UTSW	2	85,409,882 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTGACATTTCCCCATTGCTG -3'
(R):5'- GCACCAGTTAGGAAGTGCTTC -3'

Sequencing Primer
(F):5'- CTGACACCCACATAAATAAGTTGGTG -3'
(R):5'- GAAGTGCTTCTTCTCCAAAGTTC -3'

Posted On

2022-10-06