Incidental Mutation 'R9690:Olfm3'
| ID |
729096 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Olfm3
|
| Ensembl Gene |
ENSMUSG00000027965 |
| Gene Name |
olfactomedin 3 |
| Synonyms |
B230206G02Rik, optimedin |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.156)
|
| Stock # |
R9690 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
3 |
| Chromosomal Location |
114697727-114919371 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 114890593 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Leucine to Phenylalanine
at position 115
(L115F)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000060985
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000051309]
[ENSMUST00000081752]
[ENSMUST00000149158]
|
| AlphaFold |
P63056 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000051309
AA Change: L115F
PolyPhen 2
Score 0.386 (Sensitivity: 0.90; Specificity: 0.89)
|
| SMART Domains |
Protein: ENSMUSP00000060985
Gene: ENSMUSG00000027965
AA Change: L115F
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
16 |
N/A |
INTRINSIC |
|
Pfam:Noelin-1
|
46 |
145 |
3.9e-52 |
PFAM |
|
Blast:OLF
|
162 |
207 |
3e-8 |
BLAST |
|
OLF
|
220 |
470 |
4.33e-114 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000081752
AA Change: L95F
PolyPhen 2
Score 0.314 (Sensitivity: 0.90; Specificity: 0.89)
|
| SMART Domains |
Protein: ENSMUSP00000080448
Gene: ENSMUSG00000027965
AA Change: L95F
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
24 |
N/A |
INTRINSIC |
|
Pfam:Noelin-1
|
25 |
125 |
7.6e-54 |
PFAM |
|
Blast:OLF
|
142 |
187 |
3e-8 |
BLAST |
|
OLF
|
200 |
450 |
4.33e-114 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000149158
AA Change: L82F
PolyPhen 2
Score 0.386 (Sensitivity: 0.90; Specificity: 0.89)
|
| SMART Domains |
Protein: ENSMUSP00000121097
Gene: ENSMUSG00000027965
AA Change: L82F
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Noelin-1
|
12 |
112 |
2.5e-51 |
PFAM |
|
Blast:OLF
|
129 |
174 |
1e-8 |
BLAST |
|
Blast:OLF
|
187 |
210 |
1e-7 |
BLAST |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.7%
- 20x: 99.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 42 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| AI182371 |
T |
A |
2: 34,990,600 (GRCm39) |
E22D |
probably benign |
Het |
| Apbb2 |
C |
T |
5: 66,609,521 (GRCm39) |
R42H |
probably damaging |
Het |
| Atf2 |
A |
T |
2: 73,675,813 (GRCm39) |
S179R |
probably benign |
Het |
| Cage1 |
A |
G |
13: 38,203,141 (GRCm39) |
|
probably null |
Het |
| Ccdc121rt3 |
T |
C |
5: 112,503,300 (GRCm39) |
T135A |
probably benign |
Het |
| Cnnm1 |
A |
G |
19: 43,460,345 (GRCm39) |
T696A |
probably benign |
Het |
| Cyp4f17 |
T |
A |
17: 32,725,950 (GRCm39) |
S28T |
probably benign |
Het |
| Ddx59 |
T |
A |
1: 136,352,540 (GRCm39) |
I327K |
probably damaging |
Het |
| Ep300 |
A |
C |
15: 81,520,396 (GRCm39) |
Q1229P |
unknown |
Het |
| Epb41l1 |
A |
T |
2: 156,356,038 (GRCm39) |
I525F |
probably damaging |
Het |
| Epha8 |
G |
T |
4: 136,665,897 (GRCm39) |
L420M |
probably damaging |
Het |
| Fam83b |
A |
G |
9: 76,398,502 (GRCm39) |
I867T |
probably benign |
Het |
| Gapvd1 |
C |
T |
2: 34,618,492 (GRCm39) |
V294I |
probably damaging |
Het |
| Gm5798 |
T |
G |
14: 41,070,596 (GRCm39) |
F2C |
probably damaging |
Het |
| Grip1 |
G |
A |
10: 119,874,569 (GRCm39) |
E778K |
possibly damaging |
Het |
| Irgq |
G |
T |
7: 24,233,580 (GRCm39) |
A474S |
probably benign |
Het |
| Itih3 |
T |
C |
14: 30,640,264 (GRCm39) |
K348R |
probably benign |
Het |
| Kcnip4 |
T |
A |
5: 48,555,846 (GRCm39) |
N154I |
probably damaging |
Het |
| Letm2 |
T |
A |
8: 26,077,435 (GRCm39) |
K218N |
probably damaging |
Het |
| Mak16 |
T |
C |
8: 31,650,798 (GRCm39) |
S231G |
probably damaging |
Het |
| Med13 |
A |
T |
11: 86,169,670 (GRCm39) |
I1898K |
probably damaging |
Het |
| Mtcl2 |
A |
T |
2: 156,862,134 (GRCm39) |
D1598E |
probably benign |
Het |
| Myh13 |
C |
T |
11: 67,249,194 (GRCm39) |
L1305F |
probably damaging |
Het |
| Nynrin |
T |
C |
14: 56,108,204 (GRCm39) |
Y1104H |
probably benign |
Het |
| Or1j11 |
T |
A |
2: 36,311,530 (GRCm39) |
L40Q |
probably damaging |
Het |
| Or4k35 |
C |
T |
2: 111,099,822 (GRCm39) |
A297T |
probably damaging |
Het |
| Or5w22 |
C |
A |
2: 87,362,759 (GRCm39) |
N127K |
probably benign |
Het |
| Or8b3b |
C |
A |
9: 38,584,477 (GRCm39) |
E88* |
probably null |
Het |
| Pals1 |
T |
A |
12: 78,866,117 (GRCm39) |
V314D |
probably damaging |
Het |
| Proc |
C |
T |
18: 32,256,371 (GRCm39) |
G432D |
probably damaging |
Het |
| Ptpn20 |
T |
C |
14: 33,353,176 (GRCm39) |
V305A |
probably benign |
Het |
| Rmnd5b |
T |
C |
11: 51,518,511 (GRCm39) |
M122V |
probably benign |
Het |
| Sema4f |
A |
T |
6: 82,912,652 (GRCm39) |
N130K |
probably damaging |
Het |
| Sipa1l2 |
T |
C |
8: 126,218,996 (GRCm39) |
I114V |
probably benign |
Het |
| Spata16 |
A |
T |
3: 26,967,432 (GRCm39) |
D394V |
probably damaging |
Het |
| Spidr |
T |
C |
16: 15,958,649 (GRCm39) |
T38A |
probably damaging |
Het |
| Tnxb |
T |
C |
17: 34,936,171 (GRCm39) |
Y2703H |
probably damaging |
Het |
| Trmt11 |
A |
C |
10: 30,436,938 (GRCm39) |
D267E |
probably damaging |
Het |
| Tspyl5 |
C |
A |
15: 33,687,433 (GRCm39) |
A171S |
probably benign |
Het |
| Uchl5 |
T |
C |
1: 143,670,016 (GRCm39) |
V83A |
|
Het |
| Vmn1r87 |
A |
G |
7: 12,866,263 (GRCm39) |
I8T |
probably benign |
Het |
| Vmn2r53 |
A |
G |
7: 12,315,912 (GRCm39) |
S636P |
probably damaging |
Het |
|
| Other mutations in Olfm3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01656:Olfm3
|
APN |
3 |
114,916,282 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01686:Olfm3
|
APN |
3 |
114,916,497 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01732:Olfm3
|
APN |
3 |
114,890,649 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
IGL02539:Olfm3
|
APN |
3 |
114,895,579 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
IGL02580:Olfm3
|
APN |
3 |
114,916,157 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02999:Olfm3
|
APN |
3 |
114,916,397 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03142:Olfm3
|
APN |
3 |
114,890,679 (GRCm39) |
missense |
probably damaging |
0.97 |
|
IGL03239:Olfm3
|
APN |
3 |
114,916,243 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0361:Olfm3
|
UTSW |
3 |
114,914,622 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0373:Olfm3
|
UTSW |
3 |
114,916,454 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0505:Olfm3
|
UTSW |
3 |
114,916,330 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R0689:Olfm3
|
UTSW |
3 |
114,916,194 (GRCm39) |
missense |
probably benign |
0.28 |
|
R0973:Olfm3
|
UTSW |
3 |
114,895,635 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0973:Olfm3
|
UTSW |
3 |
114,895,635 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0974:Olfm3
|
UTSW |
3 |
114,895,635 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1253:Olfm3
|
UTSW |
3 |
114,916,418 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1293:Olfm3
|
UTSW |
3 |
114,895,579 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R1952:Olfm3
|
UTSW |
3 |
114,895,589 (GRCm39) |
missense |
probably null |
1.00 |
|
R2255:Olfm3
|
UTSW |
3 |
114,915,842 (GRCm39) |
splice site |
probably null |
|
|
R2334:Olfm3
|
UTSW |
3 |
114,895,608 (GRCm39) |
nonsense |
probably null |
|
|
R2510:Olfm3
|
UTSW |
3 |
114,915,959 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4222:Olfm3
|
UTSW |
3 |
114,883,820 (GRCm39) |
nonsense |
probably null |
|
|
R4716:Olfm3
|
UTSW |
3 |
114,874,755 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4912:Olfm3
|
UTSW |
3 |
114,895,589 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5084:Olfm3
|
UTSW |
3 |
114,698,202 (GRCm39) |
critical splice donor site |
probably null |
|
|
R5649:Olfm3
|
UTSW |
3 |
114,890,573 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5681:Olfm3
|
UTSW |
3 |
114,915,924 (GRCm39) |
missense |
probably benign |
0.28 |
|
R5861:Olfm3
|
UTSW |
3 |
114,916,052 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5924:Olfm3
|
UTSW |
3 |
114,916,187 (GRCm39) |
missense |
probably benign |
0.44 |
|
R5929:Olfm3
|
UTSW |
3 |
114,895,529 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5958:Olfm3
|
UTSW |
3 |
114,915,955 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6166:Olfm3
|
UTSW |
3 |
114,916,074 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6299:Olfm3
|
UTSW |
3 |
114,914,632 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6804:Olfm3
|
UTSW |
3 |
114,916,328 (GRCm39) |
missense |
probably benign |
0.10 |
|
R7032:Olfm3
|
UTSW |
3 |
114,883,805 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7565:Olfm3
|
UTSW |
3 |
114,916,393 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R7600:Olfm3
|
UTSW |
3 |
114,890,589 (GRCm39) |
missense |
possibly damaging |
0.65 |
|
R7976:Olfm3
|
UTSW |
3 |
114,874,794 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8070:Olfm3
|
UTSW |
3 |
114,895,604 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R8334:Olfm3
|
UTSW |
3 |
114,916,206 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R8527:Olfm3
|
UTSW |
3 |
114,916,196 (GRCm39) |
missense |
probably benign |
0.10 |
|
R8542:Olfm3
|
UTSW |
3 |
114,916,196 (GRCm39) |
missense |
probably benign |
0.10 |
|
R8835:Olfm3
|
UTSW |
3 |
114,916,061 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9063:Olfm3
|
UTSW |
3 |
114,914,582 (GRCm39) |
missense |
probably benign |
0.36 |
|
R9594:Olfm3
|
UTSW |
3 |
114,883,785 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9690:Olfm3
|
UTSW |
3 |
114,890,594 (GRCm39) |
nonsense |
probably null |
|
|
R9719:Olfm3
|
UTSW |
3 |
114,916,091 (GRCm39) |
nonsense |
probably null |
|
|
Z1088:Olfm3
|
UTSW |
3 |
114,698,317 (GRCm39) |
start gained |
probably benign |
|
|
Z1177:Olfm3
|
UTSW |
3 |
114,874,750 (GRCm39) |
missense |
probably benign |
0.01 |
|
| Predicted Primers |
PCR Primer
(F):5'- GGGTATTATCACCTTTTGAAGCCTTG -3'
(R):5'- TGGGCACAGGAATAGTTATTTCC -3'
Sequencing Primer
(F):5'- TGAGACAACAGACATTTATTTGCC -3'
(R):5'- CCAACTTAGTGACTTGCTACCTG -3'
|
| Posted On |
2022-10-06 |
Incidental Mutation