Mutagenetix > Incidental Mutation

Incidental Mutation 'R9702:Zfp108'

729620

Institutional Source

Beutler Lab

Gene Symbol

Zfp108

Ensembl Gene

ENSMUSG00000030486

Gene Name

zinc finger protein 108

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R9702 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

24254794-24262445 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 24260770 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 262 (T262I)

Ref Sequence

ENSEMBL: ENSMUSP00000145928 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072713] [ENSMUST00000205982] [ENSMUST00000206777]

AlphaFold

E9Q8I5

Predicted Effect

probably benign
Transcript: ENSMUST00000072713
AA Change: T262I

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000072496
Gene: ENSMUSG00000030486
AA Change: T262I

Domain	Start	End	E-Value	Type
KRAB	8	63	7.94e-18	SMART
low complexity region	140	153	N/A	INTRINSIC
ZnF_C2H2	281	303	1.33e-1	SMART
ZnF_C2H2	309	331	1.69e-3	SMART
ZnF_C2H2	337	359	1.3e-4	SMART
ZnF_C2H2	365	387	2.71e-2	SMART
ZnF_C2H2	393	415	5.14e-3	SMART
ZnF_C2H2	421	443	1.87e-5	SMART
ZnF_C2H2	449	471	3.44e-4	SMART
ZnF_C2H2	477	497	1.08e1	SMART
ZnF_C2H2	503	525	3.89e-3	SMART
ZnF_C2H2	531	553	2.09e-3	SMART
ZnF_C2H2	559	581	4.61e-5	SMART
ZnF_C2H2	587	609	7.9e-4	SMART
ZnF_C2H2	615	637	1.67e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205982
AA Change: T262I

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

Predicted Effect

probably benign
Transcript: ENSMUST00000206777
AA Change: T262I

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	A	G	11: 110,216,552	Y746H	probably damaging	Het
Adgrv1	T	A	13: 81,536,364	I1073F	possibly damaging	Het
Ankrd6	C	T	4: 32,810,202	R493H	possibly damaging	Het
Apob	G	T	12: 8,007,559	A2014S	probably damaging	Het
Bdnf	A	T	2: 109,723,772	M164L	possibly damaging	Het
Ccdc187	C	T	2: 26,282,210	V287M	unknown	Het
Cd164	G	A	10: 41,528,822	R192Q	probably damaging	Het
Cd84	T	C	1: 171,872,931	V205A	probably benign	Het
Cfap161	T	A	7: 83,793,444	M54L	probably benign	Het
Crim1	T	A	17: 78,374,087	V990E	probably damaging	Het
Dsc1	A	G	18: 20,094,628	F518L	probably benign	Het
Esyt1	G	A	10: 128,520,738	S346L	probably damaging	Het
Fbxo30	G	T	10: 11,290,638	C368F	probably benign	Het
Gas2l3	C	T	10: 89,414,081	A392T	probably benign	Het
Gfra4	T	A	2: 131,042,619	S22C	probably benign	Het
Gm11992	T	G	11: 9,056,568	V166G	probably benign	Het
Gm14085	T	A	2: 122,523,531	I546N	probably damaging	Het
Gp1bb	A	T	16: 18,621,134	L70Q	probably damaging	Het
Gtf2i	T	C	5: 134,246,561	T707A	probably benign	Het
Inpp5f	A	C	7: 128,676,791	D435A	possibly damaging	Het
Kynu	T	A	2: 43,589,457	M121K	probably damaging	Het
Ldb3	A	G	14: 34,577,133	V178A	probably benign	Het
Lrrc31	A	G	3: 30,681,077	L350P	probably damaging	Het
Mfsd14b	C	A	13: 65,073,600	V293L	probably benign	Het
Mrps23	C	T	11: 88,210,172	T121M	probably damaging	Het
Nlrp4c	T	A	7: 6,065,802	V234E	probably benign	Het
Olfr585	T	C	7: 103,098,136	Y132H	probably damaging	Het
Pign	A	T	1: 105,557,487	D782E	probably damaging	Het
Rab11b	C	A	17: 33,749,052	R82L	possibly damaging	Het
Sema3c	T	C	5: 17,653,830	S76P	probably damaging	Het
Sobp	T	C	10: 43,021,948	N547S	probably benign	Het
Tigd3	G	A	19: 5,892,808	T98M	probably damaging	Het
Tox	T	G	4: 6,697,418	M462L	probably benign	Het
Vmn1r158	T	A	7: 22,790,640	Q48L	probably benign	Het
Vps8	A	G	16: 21,644,133	T1383A	probably benign	Het
Vwa1	G	A	4: 155,772,879	P154L	probably damaging	Het

Other mutations in Zfp108

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00591:Zfp108	APN	7	24261486	missense	possibly damaging	0.82
IGL01392:Zfp108	APN	7	24258447	splice site	probably benign
R0123:Zfp108	UTSW	7	24260467	missense	probably benign	0.00
R0126:Zfp108	UTSW	7	24260724	missense	probably benign	0.01
R0134:Zfp108	UTSW	7	24260467	missense	probably benign	0.00
R0243:Zfp108	UTSW	7	24261783	missense	possibly damaging	0.81
R1227:Zfp108	UTSW	7	24260460	missense	probably benign	0.00
R1464:Zfp108	UTSW	7	24260548	missense	probably benign	0.00
R1464:Zfp108	UTSW	7	24260548	missense	probably benign	0.00
R1731:Zfp108	UTSW	7	24258539	missense	possibly damaging	0.75
R1739:Zfp108	UTSW	7	24261310	missense	probably damaging	1.00
R1751:Zfp108	UTSW	7	24261896	missense	probably damaging	1.00
R3713:Zfp108	UTSW	7	24261845	nonsense	probably null
R3839:Zfp108	UTSW	7	24260556	missense	probably benign	0.01
R3919:Zfp108	UTSW	7	24260832	missense	probably damaging	0.99
R3922:Zfp108	UTSW	7	24261348	missense	probably damaging	1.00
R4707:Zfp108	UTSW	7	24260412	missense	probably benign	0.08
R4912:Zfp108	UTSW	7	24261314	missense	probably damaging	1.00
R4965:Zfp108	UTSW	7	24260148	missense	probably benign
R4989:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5014:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5163:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5183:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5184:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5185:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5453:Zfp108	UTSW	7	24261264	missense	probably damaging	1.00
R5600:Zfp108	UTSW	7	24260586	missense	probably benign	0.00
R6494:Zfp108	UTSW	7	24261357	missense	probably damaging	1.00
R6601:Zfp108	UTSW	7	24261394	missense	probably damaging	0.98
R6735:Zfp108	UTSW	7	24261772	missense	probably damaging	1.00
R7646:Zfp108	UTSW	7	24261415	missense	probably damaging	1.00
R7732:Zfp108	UTSW	7	24261527	missense	probably benign	0.00
R7873:Zfp108	UTSW	7	24261333	missense	probably benign	0.19
R8100:Zfp108	UTSW	7	24261177	missense	probably damaging	1.00
R8313:Zfp108	UTSW	7	24260662	missense	possibly damaging	0.80
R9169:Zfp108	UTSW	7	24261498	missense	probably damaging	1.00
RF019:Zfp108	UTSW	7	24261607	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGTGATCAAGTCATCAATCCCATG -3'
(R):5'- GTAAAGTTTCTCTCCAGTGTGCAC -3'

Sequencing Primer
(F):5'- GATCAAGTCATCAATCCCATGTCATC -3'
(R):5'- ATGTTGAGATCCGAGCTGC -3'

Posted On

2022-10-06