Mutagenetix > Incidental Mutation

Incidental Mutation 'R9707:Trav7-3'

729863

Institutional Source

Beutler Lab

Gene Symbol

Trav7-3

Ensembl Gene

ENSMUSG00000096908

Gene Name

T cell receptor alpha variable 7-3

Synonyms

Gm13946

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.094) question?

Stock #

R9707 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

53680771-53681296 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 53681189 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 77 (E77G)

Ref Sequence

ENSEMBL: ENSMUSP00000137590 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000177622]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000177622
AA Change: E77G

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000137590
Gene: ENSMUSG00000096908
AA Change: E77G

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IGv	40	111	1.41e-12	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ambra1	G	A	2: 91,640,476 (GRCm39)	R635H	probably damaging	Het
Amz1	T	C	5: 140,733,949 (GRCm39)	V175A	possibly damaging	Het
Arsj	T	C	3: 126,232,160 (GRCm39)	I302T	possibly damaging	Het
Asxl3	A	G	18: 22,656,304 (GRCm39)	E1438G	probably benign	Het
BC016579	C	T	16: 45,460,760 (GRCm39)	E103K	probably damaging	Het
Ccdc54	T	A	16: 50,411,163 (GRCm39)	K34N	possibly damaging	Het
Chac2	T	C	11: 30,929,572 (GRCm39)	D55G	possibly damaging	Het
Cts3	T	A	13: 61,714,649 (GRCm39)	I219F	possibly damaging	Het
Des	A	G	1: 75,337,533 (GRCm39)	M158V	possibly damaging	Het
Dohh	T	C	10: 81,223,565 (GRCm39)	F206S	probably damaging	Het
Dst	T	C	1: 34,228,934 (GRCm39)	S2176P	probably benign	Het
Ecpas	C	T	4: 58,824,816 (GRCm39)		probably null	Het
Epb41l2	G	A	10: 25,378,235 (GRCm39)	V836M	probably damaging	Het
Foxc1	A	T	13: 31,991,882 (GRCm39)	E231V	probably damaging	Het
Ighd	T	A	12: 113,378,108 (GRCm39)	N164Y		Het
Ighv1-53	A	T	12: 115,122,035 (GRCm39)	Y113*	probably null	Het
Ildr1	A	G	16: 36,529,892 (GRCm39)	D93G	probably damaging	Het
Krt79	T	G	15: 101,839,196 (GRCm39)	E424D	probably benign	Het
Lrp1	T	C	10: 127,408,414 (GRCm39)	E1754G	probably benign	Het
Mbd4	T	A	6: 115,826,559 (GRCm39)	H144L	probably benign	Het
Mcm7	C	A	5: 138,170,000 (GRCm39)	A7S	probably benign	Het
Mmp9	T	A	2: 164,794,605 (GRCm39)	N532K	possibly damaging	Het
Msr1	T	G	8: 40,076,988 (GRCm39)	E207D	probably benign	Het
Neurl4	A	T	11: 69,799,679 (GRCm39)	D994V	probably damaging	Het
Or1e35	A	G	11: 73,798,090 (GRCm39)	V76A	possibly damaging	Het
Or6c69b	T	C	10: 129,627,444 (GRCm39)	T5A	probably damaging	Het
Or8s5	C	T	15: 98,238,154 (GRCm39)	V239I	probably benign	Het
Pcdhgb6	T	C	18: 37,876,768 (GRCm39)	I492T	possibly damaging	Het
Pde4c	A	G	8: 71,202,701 (GRCm39)	D669G	probably benign	Het
Phf3	C	A	1: 30,868,923 (GRCm39)		probably null	Het
Ptpn12	T	A	5: 21,207,620 (GRCm39)	T279S	probably damaging	Het
Rbbp6	A	T	7: 122,589,061 (GRCm39)	T287S	probably damaging	Het
Slc22a21	T	C	11: 53,851,186 (GRCm39)	N192S	probably benign	Het
Slc46a3	G	T	5: 147,821,022 (GRCm39)	H377N	probably benign	Het
Smg9	G	T	7: 24,102,869 (GRCm39)	R16L	probably damaging	Het
Spmip2	A	G	3: 79,313,167 (GRCm39)	I80M	probably damaging	Het
Synj1	A	G	16: 90,758,300 (GRCm39)	V848A	possibly damaging	Het
Taf7	A	T	18: 37,776,053 (GRCm39)	S171R	probably damaging	Het
Usp16	T	C	16: 87,263,235 (GRCm39)	S146P	probably benign	Het
Vmn1r22	A	T	6: 57,877,890 (GRCm39)	I29K	probably benign	Het
Zfp236	A	G	18: 82,664,328 (GRCm39)	C486R	probably damaging	Het
Zfp329	T	C	7: 12,544,129 (GRCm39)	Q465R	probably benign	Het

Other mutations in Trav7-3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R4051:Trav7-3	UTSW	14	53,681,203 (GRCm39)	missense	probably benign	0.32
R4052:Trav7-3	UTSW	14	53,681,203 (GRCm39)	missense	probably benign	0.32
R4207:Trav7-3	UTSW	14	53,681,203 (GRCm39)	missense	probably benign	0.32
R4208:Trav7-3	UTSW	14	53,681,203 (GRCm39)	missense	probably benign	0.32
R5261:Trav7-3	UTSW	14	53,681,207 (GRCm39)	missense	probably benign	0.05
R7809:Trav7-3	UTSW	14	53,681,072 (GRCm39)	missense	probably benign	0.00
R8199:Trav7-3	UTSW	14	53,681,099 (GRCm39)	missense	possibly damaging	0.52

Predicted Primers

PCR Primer
(F):5'- CCAGTCTTGATTGTCTGGCC -3'
(R):5'- AGAATGTCTTCTACTCTGTGGTC -3'

Sequencing Primer
(F):5'- CCTTTGTCTTTCTGCACAGGGG -3'
(R):5'- CTACTCTGTGGTCTGTGATTTATGTC -3'

Posted On

2022-10-06