Mutagenetix > Incidental Mutation

Incidental Mutation 'R9709:Ttc28'

729963

Institutional Source

Beutler Lab

Gene Symbol

Ttc28

Ensembl Gene

ENSMUSG00000033209

Gene Name

tetratricopeptide repeat domain 28

Synonyms

TPRBK, 2310015L07Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9709 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111027669-111437646 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 111433637 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Cysteine at position 2224 (S2224C)

Ref Sequence

ENSEMBL: ENSMUSP00000136116 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040111] [ENSMUST00000156290]

AlphaFold

Q80XJ3

Predicted Effect

probably damaging
Transcript: ENSMUST00000040111
AA Change: S2224C

PolyPhen 2 Score 0.969 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000136116
Gene: ENSMUSG00000033209
AA Change: S2224C

Domain	Start	End	E-Value	Type
low complexity region	4	28	N/A	INTRINSIC
TPR	52	85	2.84e1	SMART
TPR	86	119	5.03e-1	SMART
TPR	120	153	2.11e-3	SMART
TPR	268	301	8.51e0	SMART
TPR	339	372	1.78e-1	SMART
TPR	379	412	2.82e-4	SMART
TPR	419	452	9.98e-5	SMART
TPR	459	492	1.88e0	SMART
TPR	499	532	1.11e1	SMART
TPR	539	572	2.93e-2	SMART
TPR	579	612	1.21e-3	SMART
TPR	619	652	4.91e-4	SMART
TPR	659	692	7.56e-5	SMART
TPR	699	732	8.29e0	SMART
TPR	739	772	1.63e0	SMART
TPR	779	812	1.24e0	SMART
TPR	819	852	7.98e-4	SMART
TPR	859	892	8.74e0	SMART
TPR	902	935	5.43e-6	SMART
TPR	942	975	4.09e-1	SMART
TPR	982	1015	9.98e-5	SMART
TPR	1022	1055	7.12e-1	SMART
TPR	1062	1095	5.69e0	SMART
TPR	1102	1135	3.14e-2	SMART
TPR	1142	1175	2.84e1	SMART
low complexity region	1259	1277	N/A	INTRINSIC
Pfam:CHAT	1415	1738	7.3e-77	PFAM
low complexity region	1972	1990	N/A	INTRINSIC
low complexity region	2014	2031	N/A	INTRINSIC
low complexity region	2033	2045	N/A	INTRINSIC
low complexity region	2155	2171	N/A	INTRINSIC
low complexity region	2283	2293	N/A	INTRINSIC
low complexity region	2327	2352	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000156290
AA Change: S2193C

PolyPhen 2 Score 0.460 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000137609
Gene: ENSMUSG00000033209
AA Change: S2193C

Domain	Start	End	E-Value	Type
low complexity region	4	28	N/A	INTRINSIC
TPR	52	85	2.84e1	SMART
TPR	86	119	5.03e-1	SMART
TPR	120	153	2.11e-3	SMART
TPR	268	301	8.51e0	SMART
TPR	308	341	1.78e-1	SMART
TPR	348	381	2.82e-4	SMART
TPR	388	421	9.98e-5	SMART
TPR	428	461	1.88e0	SMART
TPR	468	501	1.11e1	SMART
TPR	508	541	2.93e-2	SMART
TPR	548	581	1.21e-3	SMART
TPR	588	621	4.91e-4	SMART
TPR	628	661	7.56e-5	SMART
TPR	668	701	8.29e0	SMART
TPR	708	741	1.63e0	SMART
TPR	748	781	1.24e0	SMART
TPR	788	821	7.98e-4	SMART
TPR	828	861	8.74e0	SMART
TPR	871	904	5.43e-6	SMART
TPR	911	944	4.09e-1	SMART
TPR	951	984	9.98e-5	SMART
TPR	991	1024	7.12e-1	SMART
TPR	1031	1064	5.69e0	SMART
TPR	1071	1104	3.14e-2	SMART
TPR	1111	1144	2.84e1	SMART
low complexity region	1228	1246	N/A	INTRINSIC
Pfam:CHAT	1384	1707	1.1e-76	PFAM
low complexity region	1941	1959	N/A	INTRINSIC
low complexity region	1983	2000	N/A	INTRINSIC
low complexity region	2002	2014	N/A	INTRINSIC
low complexity region	2124	2140	N/A	INTRINSIC
low complexity region	2252	2262	N/A	INTRINSIC
low complexity region	2296	2321	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810062G17Rik	T	C	3: 36,530,356 (GRCm39)	S25P	unknown	Het
4921539E11Rik	A	G	4: 103,092,678 (GRCm39)	I268T	unknown	Het
4930507D05Rik	T	A	10: 62,285,581 (GRCm39)	C102S	unknown	Het
Abca14	T	A	7: 119,888,739 (GRCm39)	Y1228*	probably null	Het
Abca17	T	C	17: 24,517,934 (GRCm39)	I792V	probably benign	Het
Abca6	C	A	11: 110,102,589 (GRCm39)	L878F	probably benign	Het
Adk	C	A	14: 21,126,386 (GRCm39)	T4N	probably benign	Het
Ankrd40cl	G	A	11: 94,175,560 (GRCm39)	S65N	probably benign	Het
Atg2b	A	T	12: 105,611,140 (GRCm39)	F1264I	probably damaging	Het
Atl3	T	G	19: 7,507,921 (GRCm39)	S358A	probably benign	Het
Atp8a2	T	A	14: 60,271,187 (GRCm39)	Y248F	probably damaging	Het
Bcas3	T	C	11: 85,474,749 (GRCm39)	V785A	probably damaging	Het
Cckar	C	T	5: 53,860,201 (GRCm39)		probably null	Het
Cdc42se2	A	G	11: 54,614,417 (GRCm39)	F47L	probably benign	Het
Cfap58	T	C	19: 47,963,992 (GRCm39)	L540P	probably damaging	Het
Copg1	A	G	6: 87,868,957 (GRCm39)	E117G	probably benign	Het
Cstpp1	T	C	2: 91,112,099 (GRCm39)	Q250R	probably benign	Het
Cul2	C	T	18: 3,431,560 (GRCm39)	T655M	probably damaging	Het
Ddi2	T	C	4: 141,412,429 (GRCm39)	Q161R	probably benign	Het
Dffb	T	C	4: 154,059,121 (GRCm39)	Y52C	probably damaging	Het
Dll1	T	C	17: 15,591,198 (GRCm39)	Q249R	probably benign	Het
Dnmt3a	T	A	12: 3,957,701 (GRCm39)	I894N	probably damaging	Het
Fshr	G	A	17: 89,293,265 (GRCm39)	T471I	probably damaging	Het
Gabpb1	C	T	2: 126,500,488 (GRCm39)	V4I	probably benign	Het
Gbp9	T	C	5: 105,231,542 (GRCm39)	Q348R	probably damaging	Het
Gm21149	G	A	5: 15,677,110 (GRCm39)	S248F	unknown	Het
Gpr152	C	A	19: 4,192,640 (GRCm39)	H60Q	probably benign	Het
Hdac9	A	G	12: 34,362,602 (GRCm39)	S612P	probably benign	Het
Hhipl2	A	G	1: 183,199,747 (GRCm39)	D102G	possibly damaging	Het
Hspa9	A	G	18: 35,073,294 (GRCm39)	V530A	possibly damaging	Het
Igfbp7	T	C	5: 77,549,384 (GRCm39)	N173S	unknown	Het
Ighv1-75	A	G	12: 115,797,791 (GRCm39)	S44P	possibly damaging	Het
Iqgap3	C	A	3: 88,016,176 (GRCm39)	F986L	probably damaging	Het
Itgax	G	T	7: 127,735,500 (GRCm39)	G523W	probably damaging	Het
Itih3	A	G	14: 30,637,587 (GRCm39)		probably null	Het
Kcnb2	T	C	1: 15,780,523 (GRCm39)	I465T	probably benign	Het
Kmt2b	A	G	7: 30,279,228 (GRCm39)	V1478A	probably damaging	Het
Ky	A	T	9: 102,419,411 (GRCm39)	I473F	probably damaging	Het
Lbr	A	G	1: 181,666,034 (GRCm39)	V25A	probably damaging	Het
Ldlr	A	G	9: 21,657,135 (GRCm39)	T751A	probably benign	Het
Lrrtm4	A	G	6: 80,786,154 (GRCm39)	E587G	probably damaging	Het
Lvrn	A	C	18: 47,006,847 (GRCm39)		probably null	Het
Mast2	A	T	4: 116,173,044 (GRCm39)	C594S	probably damaging	Het
Mast4	A	G	13: 102,910,711 (GRCm39)	V644A	probably damaging	Het
Mcm5	T	C	8: 75,842,604 (GRCm39)	Y293H	probably damaging	Het
Mthfd2	A	T	6: 83,283,665 (GRCm39)	V339E	possibly damaging	Het
Muc4	A	T	16: 32,590,644 (GRCm39)	I881F		Het
Myo7a	A	G	7: 97,743,536 (GRCm39)	S372P	possibly damaging	Het
Nbea	T	A	3: 55,693,879 (GRCm39)	K2180*	probably null	Het
Neb	T	C	2: 52,101,507 (GRCm39)	D4621G	probably damaging	Het
Nefh	C	T	11: 4,890,042 (GRCm39)	S859N	probably benign	Het
Nek2	T	A	1: 191,563,289 (GRCm39)	H384Q	possibly damaging	Het
Nfkb2	A	G	19: 46,298,782 (GRCm39)	E645G	probably benign	Het
Nin	G	T	12: 70,149,468 (GRCm39)	P47Q		Het
Nit1	C	A	1: 171,171,307 (GRCm39)	K178N	probably benign	Het
Nrap	C	A	19: 56,317,452 (GRCm39)	K1433N	probably damaging	Het
Nrap	T	C	19: 56,317,453 (GRCm39)	K1433R	probably benign	Het
Nup85	A	G	11: 115,457,463 (GRCm39)	Y55C	possibly damaging	Het
Or2a14	G	T	6: 43,130,469 (GRCm39)	V77F	possibly damaging	Het
Pde1b	A	G	15: 103,411,985 (GRCm39)	K29E	probably benign	Het
Pheta2	G	T	15: 82,227,537 (GRCm39)	D19Y	probably damaging	Het
Pheta2	C	G	15: 82,227,535 (GRCm39)	A18G	probably damaging	Het
Pik3cg	A	T	12: 32,226,687 (GRCm39)	Y1067N	probably benign	Het
Pkd1l1	C	T	11: 8,799,016 (GRCm39)	G2249S	probably damaging	Het
Plppr4	T	A	3: 117,121,976 (GRCm39)	T201S	possibly damaging	Het
Polr1e	A	G	4: 45,018,678 (GRCm39)	T3A	probably benign	Het
Polr1has	A	T	17: 37,275,249 (GRCm39)	R7S	probably benign	Het
Pou6f2	G	T	13: 18,414,389 (GRCm39)	Q129K	unknown	Het
Ppil4	T	A	10: 7,675,341 (GRCm39)	D163E	probably benign	Het
Pradc1	A	G	6: 85,424,952 (GRCm39)	F82L	probably benign	Het
Psd3	T	C	8: 68,194,414 (GRCm39)	K784R	probably null	Het
Ptgs1	C	A	2: 36,141,204 (GRCm39)	S550R	probably damaging	Het
Pxylp1	A	G	9: 96,711,030 (GRCm39)	L153P	probably damaging	Het
Rgs10	A	C	7: 127,975,729 (GRCm39)	F146C	probably damaging	Het
Rimbp2	G	A	5: 128,874,875 (GRCm39)	P239S	probably damaging	Het
Rp1	A	G	1: 4,112,255 (GRCm39)	Y1199H	unknown	Het
Rrp12	A	T	19: 41,857,231 (GRCm39)	M1181K	probably benign	Het
Rundc3b	T	C	5: 8,570,982 (GRCm39)	N279S	probably benign	Het
Sbf2	G	T	7: 110,027,514 (GRCm39)	P494Q	probably damaging	Het
Scnn1b	A	G	7: 121,509,693 (GRCm39)	T281A	probably benign	Het
Scube2	A	T	7: 109,430,971 (GRCm39)	N409K	probably damaging	Het
Serpina3m	A	C	12: 104,359,008 (GRCm39)	D340A	probably damaging	Het
Sez6	G	A	11: 77,865,121 (GRCm39)	E623K	possibly damaging	Het
Sgpp2	T	C	1: 78,367,200 (GRCm39)	I111T	probably benign	Het
Shprh	C	T	10: 11,038,574 (GRCm39)	T443I	possibly damaging	Het
Sis	T	A	3: 72,799,074 (GRCm39)	Y1726F	possibly damaging	Het
Slc17a9	T	C	2: 180,374,321 (GRCm39)	L129P	probably damaging	Het
Slc1a5	T	A	7: 16,527,729 (GRCm39)	F342I	probably benign	Het
Slc4a4	T	A	5: 89,188,205 (GRCm39)		probably null	Het
Slco3a1	T	C	7: 73,952,957 (GRCm39)	E534G	possibly damaging	Het
Smok2b	A	T	17: 13,454,052 (GRCm39)	I71F	possibly damaging	Het
Tmbim7	A	T	5: 3,711,809 (GRCm39)	H18L	probably damaging	Het
Tmem151a	A	T	19: 5,131,876 (GRCm39)	Y443*	probably null	Het
Tnks1bp1	T	G	2: 84,902,125 (GRCm39)	S1674A	probably benign	Het
Trappc11	T	A	8: 47,946,348 (GRCm39)	I1095F	probably damaging	Het
Trim11	A	G	11: 58,872,864 (GRCm39)	R183G	possibly damaging	Het
Trim29	A	G	9: 43,231,797 (GRCm39)	D348G	probably benign	Het
Vps13d	C	T	4: 144,875,915 (GRCm39)	V1537M		Het
Zfp1005	T	A	2: 150,110,305 (GRCm39)	C332S	possibly damaging	Het
Zfp184	A	G	13: 22,143,665 (GRCm39)	D457G	possibly damaging	Het

Other mutations in Ttc28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00516:Ttc28	APN	5	111,373,554 (GRCm39)	missense	probably damaging	1.00
IGL00963:Ttc28	APN	5	111,434,255 (GRCm39)	nonsense	probably null
IGL00969:Ttc28	APN	5	111,373,606 (GRCm39)	missense	probably benign	0.00
IGL01366:Ttc28	APN	5	111,233,037 (GRCm39)	critical splice donor site	probably null
IGL01528:Ttc28	APN	5	111,249,826 (GRCm39)	splice site	probably benign
IGL01558:Ttc28	APN	5	111,431,828 (GRCm39)	missense	probably damaging	0.99
IGL01973:Ttc28	APN	5	111,372,101 (GRCm39)	missense	possibly damaging	0.88
IGL02040:Ttc28	APN	5	111,040,802 (GRCm39)	nonsense	probably null
IGL02432:Ttc28	APN	5	111,371,101 (GRCm39)	missense	probably damaging	1.00
IGL02531:Ttc28	APN	5	111,373,716 (GRCm39)	missense	probably damaging	1.00
IGL02819:Ttc28	APN	5	111,414,449 (GRCm39)	missense	probably benign
IGL02830:Ttc28	APN	5	111,434,105 (GRCm39)	missense	probably benign	0.10
IGL02893:Ttc28	APN	5	111,433,251 (GRCm39)	missense	possibly damaging	0.87
IGL03387:Ttc28	APN	5	111,381,208 (GRCm39)	missense	probably benign	0.07
PIT4131001:Ttc28	UTSW	5	111,040,719 (GRCm39)	missense	probably benign	0.00
R0142:Ttc28	UTSW	5	111,425,323 (GRCm39)	missense	probably benign	0.40
R0166:Ttc28	UTSW	5	111,373,500 (GRCm39)	missense	probably benign	0.01
R0328:Ttc28	UTSW	5	111,431,933 (GRCm39)	splice site	probably benign
R0582:Ttc28	UTSW	5	111,331,162 (GRCm39)	missense	probably damaging	1.00
R0744:Ttc28	UTSW	5	111,378,947 (GRCm39)	missense	probably damaging	1.00
R0811:Ttc28	UTSW	5	111,383,366 (GRCm39)	missense	probably benign	0.24
R0812:Ttc28	UTSW	5	111,383,366 (GRCm39)	missense	probably benign	0.24
R0828:Ttc28	UTSW	5	111,371,312 (GRCm39)	missense	probably damaging	1.00
R0833:Ttc28	UTSW	5	111,378,947 (GRCm39)	missense	probably damaging	1.00
R1013:Ttc28	UTSW	5	111,424,831 (GRCm39)	missense	probably benign	0.01
R1168:Ttc28	UTSW	5	111,378,977 (GRCm39)	missense	probably damaging	1.00
R1194:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1195:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1195:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1195:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1196:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1205:Ttc28	UTSW	5	111,433,635 (GRCm39)	missense	probably benign	0.04
R1386:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1467:Ttc28	UTSW	5	111,433,254 (GRCm39)	missense	probably benign	0.00
R1467:Ttc28	UTSW	5	111,433,254 (GRCm39)	missense	probably benign	0.00
R1537:Ttc28	UTSW	5	111,433,184 (GRCm39)	missense	probably damaging	0.96
R1539:Ttc28	UTSW	5	111,248,677 (GRCm39)	missense	possibly damaging	0.77
R1558:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1560:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1561:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1566:Ttc28	UTSW	5	111,373,543 (GRCm39)	missense	probably damaging	1.00
R1768:Ttc28	UTSW	5	111,425,034 (GRCm39)	missense	possibly damaging	0.77
R1775:Ttc28	UTSW	5	111,424,677 (GRCm39)	missense	probably benign	0.00
R1909:Ttc28	UTSW	5	111,431,920 (GRCm39)	critical splice donor site	probably null
R1911:Ttc28	UTSW	5	111,428,616 (GRCm39)	missense	possibly damaging	0.93
R1970:Ttc28	UTSW	5	111,383,501 (GRCm39)	missense	probably benign	0.00
R1990:Ttc28	UTSW	5	111,424,188 (GRCm39)	missense	probably benign	0.37
R1992:Ttc28	UTSW	5	111,424,188 (GRCm39)	missense	probably benign	0.37
R2066:Ttc28	UTSW	5	111,373,799 (GRCm39)	missense	probably benign	0.01
R2112:Ttc28	UTSW	5	111,424,139 (GRCm39)	missense	probably damaging	0.99
R2158:Ttc28	UTSW	5	111,325,483 (GRCm39)	intron	probably benign
R2192:Ttc28	UTSW	5	111,371,362 (GRCm39)	missense	probably damaging	0.99
R2267:Ttc28	UTSW	5	111,373,869 (GRCm39)	missense	possibly damaging	0.75
R2384:Ttc28	UTSW	5	111,424,074 (GRCm39)	missense	possibly damaging	0.95
R2989:Ttc28	UTSW	5	111,371,881 (GRCm39)	missense	probably benign	0.29
R3881:Ttc28	UTSW	5	111,331,106 (GRCm39)	missense	probably damaging	1.00
R3919:Ttc28	UTSW	5	111,433,245 (GRCm39)	missense	possibly damaging	0.80
R4455:Ttc28	UTSW	5	111,371,924 (GRCm39)	frame shift	probably null
R4456:Ttc28	UTSW	5	111,371,924 (GRCm39)	frame shift	probably null
R4522:Ttc28	UTSW	5	111,428,038 (GRCm39)	missense	probably benign	0.01
R4548:Ttc28	UTSW	5	111,419,090 (GRCm39)	missense	possibly damaging	0.86
R4591:Ttc28	UTSW	5	111,371,147 (GRCm39)	missense	probably damaging	1.00
R4633:Ttc28	UTSW	5	111,371,867 (GRCm39)	missense	probably damaging	1.00
R4700:Ttc28	UTSW	5	111,424,909 (GRCm39)	missense	probably damaging	1.00
R4714:Ttc28	UTSW	5	111,433,095 (GRCm39)	missense	possibly damaging	0.65
R4790:Ttc28	UTSW	5	111,372,083 (GRCm39)	missense	possibly damaging	0.94
R4803:Ttc28	UTSW	5	111,425,329 (GRCm39)	missense	possibly damaging	0.90
R4840:Ttc28	UTSW	5	111,433,947 (GRCm39)	missense	probably damaging	1.00
R4969:Ttc28	UTSW	5	111,424,121 (GRCm39)	missense	probably damaging	0.96
R5019:Ttc28	UTSW	5	111,249,930 (GRCm39)	missense	possibly damaging	0.47
R5130:Ttc28	UTSW	5	111,040,722 (GRCm39)	missense	probably benign
R5150:Ttc28	UTSW	5	111,373,555 (GRCm39)	missense	probably damaging	1.00
R5214:Ttc28	UTSW	5	111,325,489 (GRCm39)	intron	probably benign
R5254:Ttc28	UTSW	5	111,419,104 (GRCm39)	missense	probably benign	0.01
R5518:Ttc28	UTSW	5	111,373,794 (GRCm39)	missense	probably benign	0.17
R5851:Ttc28	UTSW	5	111,383,335 (GRCm39)	splice site	probably benign
R5931:Ttc28	UTSW	5	111,232,975 (GRCm39)	missense	possibly damaging	0.81
R6011:Ttc28	UTSW	5	111,434,309 (GRCm39)	missense	probably benign
R6176:Ttc28	UTSW	5	111,371,851 (GRCm39)	missense	probably damaging	1.00
R6221:Ttc28	UTSW	5	111,419,114 (GRCm39)	missense	probably benign	0.00
R6398:Ttc28	UTSW	5	111,424,142 (GRCm39)	missense	probably damaging	1.00
R6717:Ttc28	UTSW	5	111,433,302 (GRCm39)	missense	probably benign
R6770:Ttc28	UTSW	5	111,434,006 (GRCm39)	missense	probably damaging	1.00
R6901:Ttc28	UTSW	5	111,424,891 (GRCm39)	missense	possibly damaging	0.88
R7038:Ttc28	UTSW	5	111,414,445 (GRCm39)	missense	probably benign	0.09
R7073:Ttc28	UTSW	5	111,371,282 (GRCm39)	missense	possibly damaging	0.96
R7101:Ttc28	UTSW	5	111,232,958 (GRCm39)	missense	probably damaging	1.00
R7135:Ttc28	UTSW	5	111,427,873 (GRCm39)	missense	probably damaging	1.00
R7350:Ttc28	UTSW	5	111,373,903 (GRCm39)	missense	probably damaging	0.97
R7454:Ttc28	UTSW	5	111,433,350 (GRCm39)	missense	probably benign	0.19
R7461:Ttc28	UTSW	5	111,371,995 (GRCm39)	missense	probably damaging	1.00
R7596:Ttc28	UTSW	5	111,427,990 (GRCm39)	missense	probably damaging	1.00
R7613:Ttc28	UTSW	5	111,371,995 (GRCm39)	missense	probably damaging	1.00
R7625:Ttc28	UTSW	5	111,433,085 (GRCm39)	missense	possibly damaging	0.65
R7648:Ttc28	UTSW	5	111,331,258 (GRCm39)	missense	possibly damaging	0.52
R7735:Ttc28	UTSW	5	111,414,544 (GRCm39)	splice site	probably null
R8030:Ttc28	UTSW	5	111,433,922 (GRCm39)	missense	possibly damaging	0.81
R8205:Ttc28	UTSW	5	111,373,596 (GRCm39)	missense	possibly damaging	0.95
R8246:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8247:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8269:Ttc28	UTSW	5	111,425,325 (GRCm39)	missense	probably benign	0.09
R8292:Ttc28	UTSW	5	111,371,123 (GRCm39)	missense	probably damaging	1.00
R8346:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8356:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8423:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8424:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8426:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8441:Ttc28	UTSW	5	111,325,507 (GRCm39)	nonsense	probably null
R8494:Ttc28	UTSW	5	111,383,506 (GRCm39)	missense	probably damaging	0.96
R8508:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8510:Ttc28	UTSW	5	111,381,207 (GRCm39)	missense	probably benign	0.33
R8729:Ttc28	UTSW	5	111,383,509 (GRCm39)	critical splice donor site	probably null
R8845:Ttc28	UTSW	5	111,372,041 (GRCm39)	missense	probably benign	0.11
R9003:Ttc28	UTSW	5	111,424,896 (GRCm39)	missense	probably benign	0.00
R9185:Ttc28	UTSW	5	111,371,342 (GRCm39)	missense	probably benign	0.03
R9187:Ttc28	UTSW	5	111,249,902 (GRCm39)	missense	probably damaging	1.00
R9245:Ttc28	UTSW	5	111,325,525 (GRCm39)	missense	unknown
R9251:Ttc28	UTSW	5	111,040,698 (GRCm39)	missense	possibly damaging	0.47
R9372:Ttc28	UTSW	5	111,331,073 (GRCm39)	missense	probably benign	0.25
R9466:Ttc28	UTSW	5	111,330,895 (GRCm39)	missense	probably damaging	0.99
R9563:Ttc28	UTSW	5	111,371,092 (GRCm39)	missense	probably benign	0.22
R9606:Ttc28	UTSW	5	111,433,140 (GRCm39)	missense	probably benign	0.00
R9691:Ttc28	UTSW	5	111,431,879 (GRCm39)	missense	probably benign	0.01
V8831:Ttc28	UTSW	5	111,248,578 (GRCm39)	missense	probably benign	0.11
Z1088:Ttc28	UTSW	5	111,434,181 (GRCm39)	missense	probably benign	0.00
Z1176:Ttc28	UTSW	5	111,414,432 (GRCm39)	missense	possibly damaging	0.59
Z1177:Ttc28	UTSW	5	111,433,605 (GRCm39)	missense	probably benign	0.10
Z1177:Ttc28	UTSW	5	111,426,452 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGACCGACAGCACTGTGAAG -3'
(R):5'- GTACTTGAGTCTGAAGAGTGGC -3'

Sequencing Primer
(F):5'- AGCACTGTGAAGTCCCAAG -3'
(R):5'- AGACTGGAGTGTCACGGC -3'

Posted On

2022-10-06