Mutagenetix > Incidental Mutation

Incidental Mutation 'R9711:Zfp442'

730130

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R9711 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150407141-150451486 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 150408287 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 565 (H565L)

Ref Sequence

ENSEMBL: ENSMUSP00000105542 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916] [ENSMUST00000185796]

AlphaFold

A2AQA0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109916
AA Change: H565L

PolyPhen 2 Score 0.931 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130
AA Change: H565L

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000185796
AA Change: H508L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130
AA Change: H508L

Domain	Start	End	E-Value	Type
KRAB	3	65	1.4e-21	SMART
ZnF_C2H2	158	180	3.4e-5	SMART
ZnF_C2H2	210	232	3.9e-5	SMART
ZnF_C2H2	238	260	1e-6	SMART
ZnF_C2H2	266	288	5.6e-6	SMART
ZnF_C2H2	294	316	1.8e-5	SMART
ZnF_C2H2	322	344	1.3e-5	SMART
ZnF_C2H2	350	372	6.7e-6	SMART
ZnF_C2H2	378	400	9.6e-5	SMART
ZnF_C2H2	406	428	6.9e-7	SMART
ZnF_C2H2	434	456	7.7e-6	SMART
ZnF_C2H2	462	484	1.9e-5	SMART
ZnF_C2H2	490	512	1.5e-5	SMART
ZnF_C2H2	518	540	2e-5	SMART
ZnF_C2H2	546	568	3.5e-6	SMART
ZnF_C2H2	574	597	1.3e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc12	T	C	8: 86,548,759 (GRCm38)	D430G	probably damaging	Het
Adgrf1	T	C	17: 43,310,689 (GRCm38)	S606P	possibly damaging	Het
Afap1	A	G	5: 35,984,196 (GRCm38)	N475D	probably damaging	Het
Ahnak2	A	G	12: 112,773,034 (GRCm38)	S1535P	possibly damaging	Het
Akt1	G	T	12: 112,658,451 (GRCm38)	H194N	possibly damaging	Het
Api5	T	A	2: 94,421,467 (GRCm38)	Q396L	possibly damaging	Het
Aplp2	T	C	9: 31,172,007 (GRCm38)	N148S	probably benign	Het
Asb3	A	G	11: 31,081,400 (GRCm38)	Y340C	probably damaging	Het
Bbox1	A	T	2: 110,268,236 (GRCm38)	M332K	probably damaging	Het
Cabin1	A	T	10: 75,743,256 (GRCm38)	S449T	probably benign	Het
Carns1	A	G	19: 4,166,008 (GRCm38)	V725A	possibly damaging	Het
Ccdc129	T	G	6: 55,887,033 (GRCm38)	S115A	probably damaging	Het
Cdkn1b	A	G	6: 134,921,095 (GRCm38)	K59R	possibly damaging	Het
Cdv3	A	T	9: 103,356,340 (GRCm38)	I212K	probably damaging	Het
Clec14a	T	A	12: 58,267,646 (GRCm38)	I397F	probably damaging	Het
Cnnm1	A	G	19: 43,495,030 (GRCm38)	I950V	possibly damaging	Het
Ddi2	A	G	4: 141,695,423 (GRCm38)	M326T	probably benign	Het
Dhx30	T	C	9: 110,085,035 (GRCm38)	T1065A	probably benign	Het
Eif3c	A	T	7: 126,547,502 (GRCm38)	M808K	possibly damaging	Het
Evi2	G	T	11: 79,516,145 (GRCm38)	H201Q	possibly damaging	Het
Exoc4	A	G	6: 33,476,056 (GRCm38)	T494A	unknown	Het
Fam208b	A	G	13: 3,599,667 (GRCm38)	V44A	probably benign	Het
Fam83f	T	A	15: 80,690,618 (GRCm38)	M242K	probably damaging	Het
Fat4	T	C	3: 39,001,225 (GRCm38)	Y4198H	probably benign	Het
Fcgbp	C	A	7: 28,093,575 (GRCm38)	N1001K	probably benign	Het
Gda	G	T	19: 21,423,085 (GRCm38)	A164E	probably damaging	Het
Gm19410	T	C	8: 35,812,339 (GRCm38)	V1786A	possibly damaging	Het
Gm45871	A	G	18: 90,590,945 (GRCm38)	N82S	probably benign	Het
Gnas	T	A	2: 174,299,599 (GRCm38)	S580T	unknown	Het
Gprin1	G	A	13: 54,738,901 (GRCm38)	P520L	probably benign	Het
Gramd4	C	T	15: 86,130,550 (GRCm38)	R433C	probably damaging	Het
Ighv5-12	T	A	12: 113,702,338 (GRCm38)	T47S	probably benign	Het
Kdm5a	T	A	6: 120,390,697 (GRCm38)	L451Q	probably damaging	Het
Kif12	T	A	4: 63,165,889 (GRCm38)	R624S	probably benign	Het
Lepr	T	A	4: 101,735,654 (GRCm38)	Y155*	probably null	Het
Lnx2	T	A	5: 147,024,566 (GRCm38)	I519F	probably damaging	Het
March7	T	A	2: 60,229,831 (GRCm38)	S101T	probably damaging	Het
Miox	A	G	15: 89,336,582 (GRCm38)	D230G	probably damaging	Het
Morc2a	T	A	11: 3,650,381 (GRCm38)	M1K	probably null	Het
Mtus1	T	C	8: 41,083,185 (GRCm38)	N498S	probably damaging	Het
Mxd1	T	A	6: 86,668,572 (GRCm38)	R46W	probably damaging	Het
Ndufa11	G	C	17: 56,717,843 (GRCm38)	A2P	possibly damaging	Het
Necab2	C	T	8: 119,471,774 (GRCm38)	H362Y	probably damaging	Het
Nphp4	G	A	4: 152,538,977 (GRCm38)	V703I	possibly damaging	Het
Nup54	T	C	5: 92,434,359 (GRCm38)	N31S	unknown	Het
Olfr1378	T	C	11: 50,969,489 (GRCm38)	L157P	probably damaging	Het
Olfr394	A	T	11: 73,887,870 (GRCm38)	C167*	probably null	Het
Olfr537-ps1	T	A	7: 140,538,584 (GRCm38)	S22R	probably benign	Het
Olfr878	A	C	9: 37,918,770 (GRCm38)	T38P	probably damaging	Het
P2rx2	T	C	5: 110,342,522 (GRCm38)	E109G	possibly damaging	Het
Pcdha7	T	C	18: 36,974,356 (GRCm38)	S145P	probably damaging	Het
Pdlim5	A	T	3: 142,242,768 (GRCm38)	V586E	probably damaging	Het
Pex5l	T	C	3: 33,082,055 (GRCm38)	E5G	probably benign	Het
Plch1	T	A	3: 63,707,755 (GRCm38)	D774V	probably damaging	Het
Plekhg3	A	T	12: 76,564,952 (GRCm38)	D335V	possibly damaging	Het
Pop1	A	G	15: 34,530,081 (GRCm38)	H905R	probably benign	Het
Ppm1k	T	C	6: 57,515,735 (GRCm38)	T189A	probably damaging	Het
Pramef12	A	G	4: 144,395,947 (GRCm38)	L9P	probably damaging	Het
Recql5	A	G	11: 115,893,541 (GRCm38)	V911A	probably damaging	Het
Rnf145	T	A	11: 44,525,003 (GRCm38)	L15*	probably null	Het
Rpl13a	A	G	7: 45,127,249 (GRCm38)	V29A	probably benign	Het
Rps6ka5	A	G	12: 100,573,991 (GRCm38)	V491A	probably benign	Het
Setbp1	T	C	18: 78,856,927 (GRCm38)	H1175R	probably benign	Het
Setd5	T	C	6: 113,116,102 (GRCm38)	S372P	probably damaging	Het
Slc1a3	T	C	15: 8,645,693 (GRCm38)	E276G	probably damaging	Het
Slc36a2	T	C	11: 55,179,343 (GRCm38)	N136S	probably benign	Het
Spag1	G	A	15: 36,190,537 (GRCm38)		probably null	Het
Spon1	A	C	7: 113,788,448 (GRCm38)	T81P	probably damaging	Het
Sv2b	A	T	7: 75,206,490 (GRCm38)	D17E	probably benign	Het
Tax1bp1	C	T	6: 52,727,230 (GRCm38)	T65I	probably damaging	Het
Tenm2	C	A	11: 36,024,514 (GRCm38)	K2065N	probably damaging	Het
Ticam2	T	C	18: 46,560,591 (GRCm38)	D143G	probably damaging	Het
Tmem132d	T	C	5: 127,792,515 (GRCm38)	E585G	possibly damaging	Het
Tnfrsf8	C	T	4: 145,293,098 (GRCm38)		probably null	Het
Trim44	C	T	2: 102,400,468 (GRCm38)	G73R	unknown	Het
Trpc3	C	T	3: 36,638,564 (GRCm38)	D760N	possibly damaging	Het
Tspan9	T	A	6: 127,965,752 (GRCm38)	M171L	probably benign	Het
Ugt2b37	A	G	5: 87,254,673 (GRCm38)	F33S	possibly damaging	Het
Vav2	A	T	2: 27,269,015 (GRCm38)	V734E	probably damaging	Het
Vmn1r214	A	G	13: 23,034,338 (GRCm38)	M1V	probably null	Het
Vmn1r24	A	T	6: 57,955,819 (GRCm38)	V238E	probably damaging	Het
Vmn2r107	A	G	17: 20,357,000 (GRCm38)	Q420R	probably damaging	Het
Vmn2r31	G	A	7: 7,384,086 (GRCm38)	P829S	probably damaging	Het
Xpo6	A	G	7: 126,113,701 (GRCm38)	F703L	probably benign	Het
Zfp950	A	T	19: 61,127,562 (GRCm38)	D2E	probably damaging	Het
Zfp992	T	A	4: 146,466,888 (GRCm38)	S355R	probably damaging	Het
Zwilch	A	T	9: 64,156,021 (GRCm38)	F309Y	probably damaging	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150,409,347 (GRCm38)	nonsense	probably null
IGL02566:Zfp442	APN	2	150,409,791 (GRCm38)	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150,409,794 (GRCm38)	splice site	probably benign
LCD18:Zfp442	UTSW	2	150,419,848 (GRCm38)	intron	probably benign
PIT4812001:Zfp442	UTSW	2	150,409,741 (GRCm38)	nonsense	probably null
R0219:Zfp442	UTSW	2	150,411,240 (GRCm38)	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150,411,249 (GRCm38)	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150,408,340 (GRCm38)	nonsense	probably null
R1702:Zfp442	UTSW	2	150,409,180 (GRCm38)	nonsense	probably null
R1829:Zfp442	UTSW	2	150,409,063 (GRCm38)	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150,408,180 (GRCm38)	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150,408,662 (GRCm38)	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150,408,122 (GRCm38)	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150,409,606 (GRCm38)	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150,408,229 (GRCm38)	missense	probably damaging	1.00
R4894:Zfp442	UTSW	2	150,411,210 (GRCm38)	critical splice donor site	probably null
R4932:Zfp442	UTSW	2	150,409,715 (GRCm38)	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150,408,495 (GRCm38)	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150,409,610 (GRCm38)	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150,408,159 (GRCm38)	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150,408,024 (GRCm38)	nonsense	probably null
R6042:Zfp442	UTSW	2	150,408,096 (GRCm38)	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150,451,401 (GRCm38)	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150,408,108 (GRCm38)	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150,409,579 (GRCm38)	missense	possibly damaging	0.72
R6931:Zfp442	UTSW	2	150,410,940 (GRCm38)	critical splice donor site	probably null
R7061:Zfp442	UTSW	2	150,408,017 (GRCm38)	missense	probably benign	0.33
R7184:Zfp442	UTSW	2	150,408,136 (GRCm38)	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150,409,281 (GRCm38)	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150,409,005 (GRCm38)	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150,408,756 (GRCm38)	missense	unknown
R7591:Zfp442	UTSW	2	150,408,172 (GRCm38)	nonsense	probably null
R7679:Zfp442	UTSW	2	150,410,997 (GRCm38)	nonsense	probably null
R7768:Zfp442	UTSW	2	150,408,321 (GRCm38)	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150,409,719 (GRCm38)	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150,409,482 (GRCm38)	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150,411,226 (GRCm38)	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150,409,176 (GRCm38)	missense	possibly damaging	0.83
R8192:Zfp442	UTSW	2	150,408,709 (GRCm38)	missense	unknown
R8528:Zfp442	UTSW	2	150,409,042 (GRCm38)	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150,408,173 (GRCm38)	missense	probably benign	0.30
R9269:Zfp442	UTSW	2	150,409,367 (GRCm38)	missense	probably benign	0.19
R9371:Zfp442	UTSW	2	150,408,756 (GRCm38)	missense	unknown
R9401:Zfp442	UTSW	2	150,409,695 (GRCm38)	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150,408,748 (GRCm38)	missense	unknown
Z1177:Zfp442	UTSW	2	150,408,479 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGAAATGTACAAAGGCTTCACCA -3'
(R):5'- GGGTTTCAAAGTGGTCTGCAAA -3'

Sequencing Primer
(F):5'- CACTTGAAGGGTTTACCACAGTGC -3'
(R):5'- TCAAAGTGGTCTGCAAAAGCATAAAC -3'

Posted On

2022-10-06