Incidental Mutation 'R9717:Dhx58'
ID 730552
Institutional Source Beutler Lab
Gene Symbol Dhx58
Ensembl Gene ENSMUSG00000017830
Gene Name DExH-box helicase 58
Synonyms D11Lgp2e, B430001I08Rik, LPG2
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.201) question?
Stock # R9717 (G1)
Quality Score 225.009
Status Not validated
Chromosome 11
Chromosomal Location 100585710-100595097 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 100592133 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Methionine to Lysine at position 305 (M305K)
Ref Sequence ENSEMBL: ENSMUSP00000017974 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000006973] [ENSMUST00000017974] [ENSMUST00000103118]
AlphaFold Q99J87
Predicted Effect probably benign
Transcript: ENSMUST00000006973
SMART Domains Protein: ENSMUSP00000006973
Gene: ENSMUSG00000020918

DomainStartEndE-ValueType
low complexity region 21 72 N/A INTRINSIC
Pfam:PCAF_N 81 332 1.2e-155 PFAM
low complexity region 398 417 N/A INTRINSIC
Pfam:Acetyltransf_7 538 621 5e-13 PFAM
Pfam:Acetyltransf_1 545 620 3.2e-11 PFAM
low complexity region 659 675 N/A INTRINSIC
BROMO 718 826 6.87e-38 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000017974
AA Change: M305K

PolyPhen 2 Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)
SMART Domains Protein: ENSMUSP00000017974
Gene: ENSMUSG00000017830
AA Change: M305K

DomainStartEndE-ValueType
DEXDc 2 207 2.86e-22 SMART
HELICc 387 475 3.85e-14 SMART
Blast:HELICc 497 543 4e-12 BLAST
Pfam:RIG-I_C-RD 552 667 1.5e-37 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000103118
SMART Domains Protein: ENSMUSP00000099407
Gene: ENSMUSG00000020918

DomainStartEndE-ValueType
low complexity region 21 72 N/A INTRINSIC
Pfam:PCAF_N 81 331 4.4e-120 PFAM
low complexity region 398 417 N/A INTRINSIC
Pfam:Acetyltransf_7 539 622 1.2e-11 PFAM
Pfam:Acetyltransf_1 547 621 3.1e-11 PFAM
low complexity region 660 676 N/A INTRINSIC
BROMO 719 827 6.87e-38 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to EMCV infection and decreased susceptibility to VSV infection. Mice homozygous for a different knock-out allele exhibit increased susceptibility to WNV infection. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 77 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adgrv1 T A 13: 81,668,900 (GRCm39) N2552I probably damaging Het
Amph G T 13: 19,309,253 (GRCm39) A444S probably benign Het
Ankrd52 A G 10: 128,216,457 (GRCm39) N157S probably benign Het
Arhgap29 T C 3: 121,797,920 (GRCm39) F537L probably benign Het
Asic1 A C 15: 99,590,657 (GRCm39) T136P probably damaging Het
Atg2b T C 12: 105,605,561 (GRCm39) Y1468C probably benign Het
Atm A G 9: 53,427,817 (GRCm39) L431P probably damaging Het
Atosb T C 4: 43,036,050 (GRCm39) H227R probably damaging Het
Btaf1 A G 19: 36,922,646 (GRCm39) T17A probably benign Het
Car10 A G 11: 93,195,367 (GRCm39) N58S probably benign Het
Cd79b A T 11: 106,202,845 (GRCm39) D252E probably damaging Het
Cdipt C T 7: 126,576,202 (GRCm39) probably benign Het
Cebpe T C 14: 54,949,165 (GRCm39) D84G probably damaging Het
Cenpj T C 14: 56,790,453 (GRCm39) E532G probably benign Het
Cherp A G 8: 73,216,920 (GRCm39) probably null Het
Chuk T C 19: 44,071,109 (GRCm39) D532G possibly damaging Het
Clec1b C T 6: 129,374,603 (GRCm39) T9I probably benign Het
Clspn G T 4: 126,458,756 (GRCm39) A280S possibly damaging Het
Cts3 T G 13: 61,712,799 (GRCm39) Y307S probably benign Het
Cyp2c39 T C 19: 39,556,493 (GRCm39) M443T possibly damaging Het
Dhx57 C T 17: 80,582,447 (GRCm39) R386H probably damaging Het
Dlgap3 T C 4: 127,129,287 (GRCm39) L894P probably damaging Het
Dnah3 A T 7: 119,574,299 (GRCm39) N2164K probably damaging Het
Dnajb14 A G 3: 137,608,044 (GRCm39) N183S probably benign Het
Drd5 G T 5: 38,478,090 (GRCm39) R361L probably damaging Het
Duoxa1 T C 2: 122,135,622 (GRCm39) E159G probably damaging Het
Exoc1 T A 5: 76,711,079 (GRCm39) S659R probably benign Het
Fbln7 T C 2: 128,719,314 (GRCm39) I37T probably benign Het
Fcgrt T G 7: 44,744,853 (GRCm39) E205A possibly damaging Het
Fcho2 A T 13: 98,900,202 (GRCm39) S304T probably damaging Het
Gbp9 C A 5: 105,253,587 (GRCm39) G43* probably null Het
Gucy2d A G 7: 98,123,868 (GRCm39) K151R probably benign Het
Heatr4 T A 12: 84,024,829 (GRCm39) I331F probably damaging Het
Hmcn1 G A 1: 150,485,378 (GRCm39) T4408I probably damaging Het
Hoxc9 C T 15: 102,890,551 (GRCm39) T156M probably benign Het
Hrnr A T 3: 93,227,987 (GRCm39) E35V probably damaging Het
Idua C T 5: 108,818,037 (GRCm39) Q70* probably null Het
Klf5 A T 14: 99,539,189 (GRCm39) I201F probably damaging Het
Lonrf1 T C 8: 36,701,164 (GRCm39) K349E probably damaging Het
Lrp1b A T 2: 41,158,395 (GRCm39) D1721E Het
Mdh1 T A 11: 21,521,870 (GRCm39) probably benign Het
Mllt11 T C 3: 95,127,521 (GRCm39) H83R probably benign Het
Mrgprb4 A C 7: 47,848,583 (GRCm39) I115S possibly damaging Het
Mrpl38 A G 11: 116,023,296 (GRCm39) F319S probably damaging Het
Naif1 A T 2: 32,344,907 (GRCm39) M204L probably benign Het
Ncan T A 8: 70,554,628 (GRCm39) D1063V probably damaging Het
Noto A T 6: 85,401,327 (GRCm39) R119W possibly damaging Het
Or4c115 G A 2: 88,927,573 (GRCm39) L233F probably benign Het
Or51h7 A T 7: 102,591,165 (GRCm39) D206E probably damaging Het
Or6c69 T A 10: 129,748,048 (GRCm39) Y33F probably damaging Het
Or8b3 A T 9: 38,314,841 (GRCm39) I224F probably damaging Het
Ovch2 A G 7: 107,393,584 (GRCm39) W181R probably damaging Het
Palm G C 10: 79,655,117 (GRCm39) G292R probably damaging Het
Pdgfrb T A 18: 61,205,787 (GRCm39) L591* probably null Het
Pik3c2g T C 6: 139,841,910 (GRCm39) S772P Het
Prx C A 7: 27,217,411 (GRCm39) D776E probably benign Het
Ptpra A G 2: 130,384,366 (GRCm39) E562G possibly damaging Het
Rbm4b A G 19: 4,807,359 (GRCm39) Y25C probably damaging Het
Reln T C 5: 22,136,427 (GRCm39) T2534A probably benign Het
Rnf123 T C 9: 107,954,963 (GRCm39) S14G probably benign Het
Rock2 A G 12: 17,015,602 (GRCm39) H833R probably benign Het
Rxfp3 A G 15: 11,037,111 (GRCm39) V87A possibly damaging Het
S100b G A 10: 76,092,936 (GRCm39) G23D probably damaging Het
Scn9a T A 2: 66,357,002 (GRCm39) M1100L probably benign Het
Septin11 T A 5: 93,296,266 (GRCm39) S55T possibly damaging Het
Speer4a3 T C 5: 26,154,829 (GRCm39) E257G probably damaging Het
Sv2b T A 7: 74,769,676 (GRCm39) Q622L probably benign Het
Taar7a T C 10: 23,868,799 (GRCm39) D194G probably benign Het
Trappc3 T C 4: 126,169,014 (GRCm39) I168T probably benign Het
Trim27 T C 13: 21,374,296 (GRCm39) probably null Het
Wdr49 G A 3: 75,304,359 (GRCm39) T109I probably benign Het
Wdr64 T C 1: 175,544,854 (GRCm39) Y96H probably damaging Het
Zfp219 A T 14: 52,247,049 (GRCm39) L26Q probably damaging Het
Zfp758 T A 17: 22,593,829 (GRCm39) V105D possibly damaging Het
Zfp975 C G 7: 42,312,332 (GRCm39) E94Q possibly damaging Het
Zfyve9 C T 4: 108,539,334 (GRCm39) A289T probably benign Het
Zscan5b T C 7: 6,234,525 (GRCm39) S184P possibly damaging Het
Other mutations in Dhx58
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01395:Dhx58 APN 11 100,594,752 (GRCm39) missense probably damaging 0.97
IGL02476:Dhx58 APN 11 100,593,090 (GRCm39) missense probably benign 0.00
R0103:Dhx58 UTSW 11 100,586,096 (GRCm39) missense probably damaging 1.00
R0103:Dhx58 UTSW 11 100,586,096 (GRCm39) missense probably damaging 1.00
R0137:Dhx58 UTSW 11 100,587,823 (GRCm39) missense probably damaging 0.99
R0164:Dhx58 UTSW 11 100,586,150 (GRCm39) missense probably benign 0.42
R0164:Dhx58 UTSW 11 100,586,150 (GRCm39) missense probably benign 0.42
R0369:Dhx58 UTSW 11 100,592,374 (GRCm39) critical splice donor site probably null
R0390:Dhx58 UTSW 11 100,590,090 (GRCm39) missense probably damaging 1.00
R0606:Dhx58 UTSW 11 100,593,077 (GRCm39) missense probably benign 0.00
R1710:Dhx58 UTSW 11 100,594,400 (GRCm39) missense probably benign 0.20
R1816:Dhx58 UTSW 11 100,593,978 (GRCm39) missense probably damaging 0.98
R1993:Dhx58 UTSW 11 100,594,316 (GRCm39) splice site probably null
R2281:Dhx58 UTSW 11 100,588,980 (GRCm39) critical splice donor site probably null
R3176:Dhx58 UTSW 11 100,587,805 (GRCm39) missense probably damaging 1.00
R3276:Dhx58 UTSW 11 100,587,805 (GRCm39) missense probably damaging 1.00
R4651:Dhx58 UTSW 11 100,592,185 (GRCm39) missense probably damaging 1.00
R4652:Dhx58 UTSW 11 100,592,185 (GRCm39) missense probably damaging 1.00
R4716:Dhx58 UTSW 11 100,587,797 (GRCm39) splice site probably null
R5030:Dhx58 UTSW 11 100,586,963 (GRCm39) missense probably damaging 1.00
R5082:Dhx58 UTSW 11 100,587,802 (GRCm39) missense probably benign 0.29
R5098:Dhx58 UTSW 11 100,585,999 (GRCm39) missense probably benign
R5394:Dhx58 UTSW 11 100,589,034 (GRCm39) missense probably benign 0.00
R5397:Dhx58 UTSW 11 100,594,746 (GRCm39) missense probably damaging 1.00
R5787:Dhx58 UTSW 11 100,592,145 (GRCm39) missense possibly damaging 0.91
R5975:Dhx58 UTSW 11 100,593,035 (GRCm39) missense probably damaging 0.98
R6310:Dhx58 UTSW 11 100,590,193 (GRCm39) missense probably benign 0.01
R6935:Dhx58 UTSW 11 100,589,232 (GRCm39) splice site probably null
R7311:Dhx58 UTSW 11 100,588,997 (GRCm39) missense probably benign
R7908:Dhx58 UTSW 11 100,586,130 (GRCm39) missense probably damaging 0.99
R8317:Dhx58 UTSW 11 100,594,388 (GRCm39) missense probably damaging 1.00
R8821:Dhx58 UTSW 11 100,594,806 (GRCm39) missense probably damaging 1.00
R8831:Dhx58 UTSW 11 100,594,806 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TTAGGAAAATAGAGACCCAGGACTC -3'
(R):5'- GAAGTTTGCGAGCCTGTCTG -3'

Sequencing Primer
(F):5'- ACTCTCCTTGGGTCCTGGTAG -3'
(R):5'- CCTTAAGGGGCGGAGTTTC -3'
Posted On 2022-10-06