|Institutional Source||Beutler Lab|
|Gene Name||Kruppel-like factor 5|
|Synonyms||IKLF, Bteb2, 4930520J07Rik, CKLF|
|Essential gene?||Essential (E-score: 1.000)|
|Stock #||R9717 (G1)|
|Chromosomal Location||99298691-99315036 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 99301753 bp (GRCm38)|
|Amino Acid Change||Isoleucine to Phenylalanine at position 201 (I201F)|
|Ref Sequence||ENSEMBL: ENSMUSP00000005279 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000005279] [ENSMUST00000226784]|
AA Change: I201F
PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
AA Change: I201F
AA Change: I121F
PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
PHENOTYPE: Homozygous null mice die during gestation, while heterozygotes exhibit abnormal cardiovascular remodeling after external stress. Mice homozygous for a floxed allele activated in the prostate exhibit increased cell proliferation and hyperplasia in the prostate without neoplasia. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Klf5||
(F):5'- CTGGCCTCTACAAATCCCAG -3'
(R):5'- ATGCCCTGGAACTGTTTCATTG -3'
(F):5'- GAGACCATGCGTAACACAGATC -3'
(R):5'- CATTGACGTCTGTGGAACAGC -3'