Incidental Mutation 'R9721:Or5b3'
ID 730819
Institutional Source Beutler Lab
Gene Symbol Or5b3
Ensembl Gene ENSMUSG00000063777
Gene Name olfactory receptor family 5 subfamily B member 3
Synonyms MOR202-11, Olfr1469, GA_x6K02T2RE5P-3743369-3744289
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.082) question?
Stock # R9721 (G1)
Quality Score 225.009
Status Not validated
Chromosome 19
Chromosomal Location 13387935-13388864 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 13388334 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 134 (T134S)
Ref Sequence ENSEMBL: ENSMUSP00000150006 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000077538] [ENSMUST00000216910]
AlphaFold Q8VFW5
Predicted Effect probably benign
Transcript: ENSMUST00000077538
AA Change: T134S

PolyPhen 2 Score 0.167 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000076741
Gene: ENSMUSG00000063777
AA Change: T134S

DomainStartEndE-ValueType
Pfam:7tm_4 32 308 2e-47 PFAM
Pfam:7tm_1 42 290 2.3e-19 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000216910
AA Change: T134S

PolyPhen 2 Score 0.167 (Sensitivity: 0.92; Specificity: 0.87)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 62 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700006A11Rik A T 3: 124,212,932 (GRCm39) Y120N probably benign Het
AAdacl4fm3 A T 4: 144,429,942 (GRCm39) V349D possibly damaging Het
Alox8 T A 11: 69,087,911 (GRCm39) H131L probably benign Het
Atp13a1 A G 8: 70,252,087 (GRCm39) E588G probably damaging Het
AU040320 G A 4: 126,733,441 (GRCm39) V654M probably damaging Het
Bicra A G 7: 15,713,101 (GRCm39) L982P probably damaging Het
Cdh11 A T 8: 103,406,257 (GRCm39) V72E probably damaging Het
Cfap65 T C 1: 74,958,501 (GRCm39) T869A probably benign Het
Cplane1 A G 15: 8,254,893 (GRCm39) T27A unknown Het
Ddx19a A G 8: 111,705,107 (GRCm39) F338S probably damaging Het
Dhrs7c T A 11: 67,705,904 (GRCm39) V219E probably damaging Het
Dhx33 A G 11: 70,892,424 (GRCm39) V115A probably damaging Het
Dst A G 1: 34,231,866 (GRCm39) D3153G probably benign Het
Ecpas A T 4: 58,850,938 (GRCm39) S412T probably benign Het
Eif5b T C 1: 38,076,740 (GRCm39) probably null Het
Ep300 A G 15: 81,492,516 (GRCm39) N284S unknown Het
Fam83a A G 15: 57,849,513 (GRCm39) N19S probably benign Het
Fgd5 A T 6: 91,965,278 (GRCm39) T504S probably benign Het
Flt4 G A 11: 49,535,260 (GRCm39) probably null Het
Ggta1 G T 2: 35,303,418 (GRCm39) D91E probably benign Het
Gls A G 1: 52,251,427 (GRCm39) V310A probably damaging Het
Gm5414 A T 15: 101,536,582 (GRCm39) C14* probably null Het
Gm9887 C G 12: 69,418,629 (GRCm39) A202P unknown Het
Ifi214 T C 1: 173,355,479 (GRCm39) T110A possibly damaging Het
Il4i1 A G 7: 44,489,113 (GRCm39) R293G probably benign Het
Itpr1 A T 6: 108,383,063 (GRCm39) T1464S probably damaging Het
Kif18a T C 2: 109,123,400 (GRCm39) S225P probably damaging Het
Kif21a A T 15: 90,855,330 (GRCm39) I678N probably damaging Het
Klf11 T A 12: 24,710,240 (GRCm39) D429E probably damaging Het
Kntc1 A G 5: 123,939,948 (GRCm39) T1581A probably benign Het
Lama2 T C 10: 27,343,338 (GRCm39) N45D possibly damaging Het
Lrrk1 T C 7: 65,924,623 (GRCm39) I1319V probably damaging Het
Ltbr G A 6: 125,284,348 (GRCm39) R365W probably damaging Het
Malrd1 C T 2: 15,701,638 (GRCm39) T751I unknown Het
Matcap1 A T 8: 106,009,820 (GRCm39) D376E probably benign Het
Mgat5b T C 11: 116,857,595 (GRCm39) L363P probably damaging Het
Mphosph9 A T 5: 124,436,738 (GRCm39) S535R possibly damaging Het
Nckap5 C T 1: 125,955,017 (GRCm39) D512N probably damaging Het
Ngef T A 1: 87,406,857 (GRCm39) D637V probably damaging Het
Nup93 T C 8: 95,030,313 (GRCm39) Y391H probably damaging Het
Or10j7 C A 1: 173,011,915 (GRCm39) V29F probably benign Het
Or4c110 T C 2: 88,832,060 (GRCm39) T191A Het
Pcdhb4 C A 18: 37,442,905 (GRCm39) D738E possibly damaging Het
Pfpl G C 19: 12,406,297 (GRCm39) E183Q probably damaging Het
Phactr3 G A 2: 177,898,043 (GRCm39) E86K probably damaging Het
Psd C T 19: 46,311,628 (GRCm39) D351N probably benign Het
Pzp A G 6: 128,472,154 (GRCm39) probably null Het
Rerg T A 6: 137,033,415 (GRCm39) K106* probably null Het
Samd9l T C 6: 3,375,854 (GRCm39) E469G possibly damaging Het
Smok2b C A 17: 13,453,865 (GRCm39) Y8* probably null Het
Spata31e3 T C 13: 50,400,688 (GRCm39) Y546C possibly damaging Het
Spn A G 7: 126,735,437 (GRCm39) S357P probably benign Het
Tek T G 4: 94,692,539 (GRCm39) W216G possibly damaging Het
Trpm3 A T 19: 22,866,762 (GRCm39) H531L probably benign Het
Trpm6 A G 19: 18,807,336 (GRCm39) M1027V probably benign Het
Tsc2 G A 17: 24,818,616 (GRCm39) R1408* probably null Het
Tut4 T A 4: 108,412,778 (GRCm39) M1493K probably benign Het
Unc13b A G 4: 43,101,869 (GRCm39) N155D probably benign Het
Vmn1r52 G A 6: 90,156,008 (GRCm39) C104Y probably damaging Het
Xylt1 A T 7: 117,148,255 (GRCm39) E273V probably damaging Het
Zc3h4 C A 7: 16,168,770 (GRCm39) Q1035K unknown Het
Zfp14 A T 7: 29,738,609 (GRCm39) S125R probably benign Het
Other mutations in Or5b3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01064:Or5b3 APN 19 13,388,590 (GRCm39) missense probably benign 0.01
IGL01520:Or5b3 APN 19 13,388,114 (GRCm39) missense probably damaging 0.97
IGL01671:Or5b3 APN 19 13,388,255 (GRCm39) missense probably benign 0.29
IGL02247:Or5b3 APN 19 13,388,831 (GRCm39) missense probably benign 0.01
IGL02297:Or5b3 APN 19 13,388,839 (GRCm39) missense probably benign 0.00
IGL02417:Or5b3 APN 19 13,388,259 (GRCm39) missense possibly damaging 0.52
IGL02442:Or5b3 APN 19 13,388,351 (GRCm39) missense probably benign 0.00
IGL02989:Or5b3 APN 19 13,388,850 (GRCm39) missense probably benign
IGL03269:Or5b3 APN 19 13,388,792 (GRCm39) missense probably damaging 0.99
IGL02988:Or5b3 UTSW 19 13,388,826 (GRCm39) missense possibly damaging 0.75
R0707:Or5b3 UTSW 19 13,388,784 (GRCm39) missense probably benign 0.22
R1055:Or5b3 UTSW 19 13,388,754 (GRCm39) missense probably benign 0.10
R1102:Or5b3 UTSW 19 13,388,454 (GRCm39) missense probably damaging 1.00
R1946:Or5b3 UTSW 19 13,388,143 (GRCm39) missense possibly damaging 0.64
R2111:Or5b3 UTSW 19 13,388,307 (GRCm39) missense probably damaging 0.99
R4072:Or5b3 UTSW 19 13,388,299 (GRCm39) missense possibly damaging 0.49
R4073:Or5b3 UTSW 19 13,388,299 (GRCm39) missense possibly damaging 0.49
R4076:Or5b3 UTSW 19 13,388,299 (GRCm39) missense possibly damaging 0.49
R4726:Or5b3 UTSW 19 13,388,469 (GRCm39) missense probably damaging 1.00
R4939:Or5b3 UTSW 19 13,388,219 (GRCm39) missense probably benign 0.10
R5914:Or5b3 UTSW 19 13,388,326 (GRCm39) missense probably benign 0.31
R6003:Or5b3 UTSW 19 13,388,403 (GRCm39) missense probably benign 0.34
R6743:Or5b3 UTSW 19 13,387,957 (GRCm39) missense probably damaging 1.00
R6825:Or5b3 UTSW 19 13,388,514 (GRCm39) missense probably benign 0.01
R6826:Or5b3 UTSW 19 13,388,452 (GRCm39) missense probably benign 0.05
R6970:Or5b3 UTSW 19 13,388,792 (GRCm39) missense probably damaging 0.99
R7558:Or5b3 UTSW 19 13,388,355 (GRCm39) missense probably damaging 1.00
R7596:Or5b3 UTSW 19 13,388,511 (GRCm39) missense probably benign 0.01
R7923:Or5b3 UTSW 19 13,388,182 (GRCm39) missense probably benign 0.17
R8014:Or5b3 UTSW 19 13,388,175 (GRCm39) missense not run
R8506:Or5b3 UTSW 19 13,388,604 (GRCm39) missense possibly damaging 0.49
R8746:Or5b3 UTSW 19 13,388,092 (GRCm39) missense probably benign 0.44
R8803:Or5b3 UTSW 19 13,388,037 (GRCm39) missense probably damaging 0.99
R9112:Or5b3 UTSW 19 13,388,475 (GRCm39) missense probably benign 0.05
Z1177:Or5b3 UTSW 19 13,388,083 (GRCm39) missense possibly damaging 0.94
Predicted Primers PCR Primer
(F):5'- CTGCTGATTGTCCTGGACTC -3'
(R):5'- TCTGTCAGAGCAAGAGAGAACC -3'

Sequencing Primer
(F):5'- GATTGTCCTGGACTCTCGTC -3'
(R):5'- AGAACCATGACTGCTGGAATATC -3'
Posted On 2022-10-06