Mutagenetix > Incidental Mutation

Incidental Mutation 'R9721:Trpm3'

730821

Institutional Source

Beutler Lab

Gene Symbol

Trpm3

Ensembl Gene

ENSMUSG00000052387

Gene Name

transient receptor potential cation channel, subfamily M, member 3

Synonyms

B930001P07Rik, 6330504P12Rik, MLSN2, melastatin 2, LTRPC3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R9721 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

22139119-22989884 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 22889398 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 531 (H531L)

Ref Sequence

ENSEMBL: ENSMUSP00000084857 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037901] [ENSMUST00000074770] [ENSMUST00000087576] [ENSMUST00000099564] [ENSMUST00000099569]

AlphaFold

J9S314

Predicted Effect

probably benign
Transcript: ENSMUST00000037901
AA Change: H529L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000042184
Gene: ENSMUSG00000052387
AA Change: H529L

Domain	Start	End	E-Value	Type
Blast:ANK	485	514	1e-6	BLAST
low complexity region	619	631	N/A	INTRINSIC
low complexity region	674	689	N/A	INTRINSIC
low complexity region	788	800	N/A	INTRINSIC
low complexity region	821	840	N/A	INTRINSIC
Pfam:Ion_trans	883	1136	1.7e-19	PFAM
Pfam:TRPM_tetra	1227	1282	1.5e-26	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000074770
AA Change: H531L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000074328
Gene: ENSMUSG00000052387
AA Change: H531L

Domain	Start	End	E-Value	Type
low complexity region	16	33	N/A	INTRINSIC
Blast:ANK	487	516	5e-7	BLAST
low complexity region	611	623	N/A	INTRINSIC
low complexity region	666	681	N/A	INTRINSIC
low complexity region	780	792	N/A	INTRINSIC
low complexity region	813	832	N/A	INTRINSIC
transmembrane domain	874	896	N/A	INTRINSIC
Pfam:Ion_trans	908	1116	5.1e-14	PFAM
low complexity region	1378	1388	N/A	INTRINSIC
low complexity region	1433	1455	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000087576
AA Change: H531L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000084857
Gene: ENSMUSG00000052387
AA Change: H531L

Domain	Start	End	E-Value	Type
low complexity region	16	33	N/A	INTRINSIC
Blast:ANK	487	516	5e-7	BLAST
low complexity region	621	633	N/A	INTRINSIC
low complexity region	676	691	N/A	INTRINSIC
low complexity region	790	802	N/A	INTRINSIC
low complexity region	823	842	N/A	INTRINSIC
transmembrane domain	884	906	N/A	INTRINSIC
Pfam:Ion_trans	918	1126	5.1e-14	PFAM
low complexity region	1388	1398	N/A	INTRINSIC
low complexity region	1443	1465	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000099564
AA Change: H383L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000097160
Gene: ENSMUSG00000052387
AA Change: H383L

Domain	Start	End	E-Value	Type
low complexity region	451	463	N/A	INTRINSIC
low complexity region	506	521	N/A	INTRINSIC
low complexity region	620	632	N/A	INTRINSIC
low complexity region	653	672	N/A	INTRINSIC
transmembrane domain	714	736	N/A	INTRINSIC
Pfam:Ion_trans	748	919	1.5e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000099569
AA Change: H531L

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000097164
Gene: ENSMUSG00000052387
AA Change: H531L

Domain	Start	End	E-Value	Type
low complexity region	16	33	N/A	INTRINSIC
Blast:ANK	487	516	6e-7	BLAST
low complexity region	609	621	N/A	INTRINSIC
low complexity region	664	679	N/A	INTRINSIC
low complexity region	778	790	N/A	INTRINSIC
low complexity region	811	830	N/A	INTRINSIC
Pfam:Ion_trans	873	1138	3.2e-19	PFAM
Pfam:TRPM_tetra	1229	1284	4.4e-26	PFAM
low complexity region	1388	1398	N/A	INTRINSIC
low complexity region	1443	1465	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The product of this gene belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation-selective channels important for cellular calcium signaling and homeostasis. The protein encoded by this gene mediates calcium entry, and this entry is potentiated by calcium store depletion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null mutation display impaired thermal and chemical nociception. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	A	T	3: 124,419,283 (GRCm38)	Y120N	probably benign	Het
2410089E03Rik	A	G	15: 8,225,409 (GRCm38)	T27A	unknown	Het
4931428F04Rik	A	T	8: 105,283,188 (GRCm38)	D376E	probably benign	Het
AI314180	A	T	4: 58,850,938 (GRCm38)	S412T	probably benign	Het
Alox8	T	A	11: 69,197,085 (GRCm38)	H131L	probably benign	Het
Atp13a1	A	G	8: 69,799,437 (GRCm38)	E588G	probably damaging	Het
AU040320	G	A	4: 126,839,648 (GRCm38)	V654M	probably damaging	Het
Bicra	A	G	7: 15,979,176 (GRCm38)	L982P	probably damaging	Het
Cdh11	A	T	8: 102,679,625 (GRCm38)	V72E	probably damaging	Het
Cfap65	T	C	1: 74,919,342 (GRCm38)	T869A	probably benign	Het
Ddx19a	A	G	8: 110,978,475 (GRCm38)	F338S	probably damaging	Het
Dhrs7c	T	A	11: 67,815,078 (GRCm38)	V219E	probably damaging	Het
Dhx33	A	G	11: 71,001,598 (GRCm38)	V115A	probably damaging	Het
Dst	A	G	1: 34,192,785 (GRCm38)	D3153G	probably benign	Het
Eif5b	T	C	1: 38,037,659 (GRCm38)		probably null	Het
Ep300	A	G	15: 81,608,315 (GRCm38)	N284S	unknown	Het
Fam83a	A	G	15: 57,986,117 (GRCm38)	N19S	probably benign	Het
Fgd5	A	T	6: 91,988,297 (GRCm38)	T504S	probably benign	Het
Flt4	G	A	11: 49,644,433 (GRCm38)		probably null	Het
Ggta1	G	T	2: 35,413,406 (GRCm38)	D91E	probably benign	Het
Gls	A	G	1: 52,212,268 (GRCm38)	V310A	probably damaging	Het
Gm13178	A	T	4: 144,703,372 (GRCm38)	V349D	possibly damaging	Het
Gm5414	A	T	15: 101,628,147 (GRCm38)	C14*	probably null	Het
Gm906	T	C	13: 50,246,652 (GRCm38)	Y546C	possibly damaging	Het
Gm9887	C	G	12: 69,371,855 (GRCm38)	A202P	unknown	Het
Ifi214	T	C	1: 173,527,913 (GRCm38)	T110A	possibly damaging	Het
Il4i1	A	G	7: 44,839,689 (GRCm38)	R293G	probably benign	Het
Itpr1	A	T	6: 108,406,102 (GRCm38)	T1464S	probably damaging	Het
Kif18a	T	C	2: 109,293,055 (GRCm38)	S225P	probably damaging	Het
Kif21a	A	T	15: 90,971,127 (GRCm38)	I678N	probably damaging	Het
Klf11	T	A	12: 24,660,241 (GRCm38)	D429E	probably damaging	Het
Kntc1	A	G	5: 123,801,885 (GRCm38)	T1581A	probably benign	Het
Lama2	T	C	10: 27,467,342 (GRCm38)	N45D	possibly damaging	Het
Lrrk1	T	C	7: 66,274,875 (GRCm38)	I1319V	probably damaging	Het
Ltbr	G	A	6: 125,307,385 (GRCm38)	R365W	probably damaging	Het
Malrd1	C	T	2: 15,696,827 (GRCm38)	T751I	unknown	Het
Mgat5b	T	C	11: 116,966,769 (GRCm38)	L363P	probably damaging	Het
Mphosph9	A	T	5: 124,298,675 (GRCm38)	S535R	possibly damaging	Het
Nckap5	C	T	1: 126,027,280 (GRCm38)	D512N	probably damaging	Het
Ngef	T	A	1: 87,479,135 (GRCm38)	D637V	probably damaging	Het
Nup93	T	C	8: 94,303,685 (GRCm38)	Y391H	probably damaging	Het
Olfr1215	T	C	2: 89,001,716 (GRCm38)	T191A		Het
Olfr1406	C	A	1: 173,184,348 (GRCm38)	V29F	probably benign	Het
Olfr1469	A	T	19: 13,410,970 (GRCm38)	T134S	probably benign	Het
Pcdhb4	C	A	18: 37,309,852 (GRCm38)	D738E	possibly damaging	Het
Pfpl	G	C	19: 12,428,933 (GRCm38)	E183Q	probably damaging	Het
Phactr3	G	A	2: 178,256,250 (GRCm38)	E86K	probably damaging	Het
Psd	C	T	19: 46,323,189 (GRCm38)	D351N	probably benign	Het
Pzp	A	G	6: 128,495,191 (GRCm38)		probably null	Het
Rerg	T	A	6: 137,056,417 (GRCm38)	K106*	probably null	Het
Samd9l	T	C	6: 3,375,854 (GRCm38)	E469G	possibly damaging	Het
Smok2b	C	A	17: 13,234,978 (GRCm38)	Y8*	probably null	Het
Spn	A	G	7: 127,136,265 (GRCm38)	S357P	probably benign	Het
Tek	T	G	4: 94,804,302 (GRCm38)	W216G	possibly damaging	Het
Trpm6	A	G	19: 18,829,972 (GRCm38)	M1027V	probably benign	Het
Tsc2	G	A	17: 24,599,642 (GRCm38)	R1408*	probably null	Het
Unc13b	A	G	4: 43,101,869 (GRCm38)	N155D	probably benign	Het
Vmn1r52	G	A	6: 90,179,026 (GRCm38)	C104Y	probably damaging	Het
Xylt1	A	T	7: 117,549,020 (GRCm38)	E273V	probably damaging	Het
Zc3h4	C	A	7: 16,434,845 (GRCm38)	Q1035K	unknown	Het
Zcchc11	T	A	4: 108,555,581 (GRCm38)	M1493K	probably benign	Het
Zfp14	A	T	7: 30,039,184 (GRCm38)	S125R	probably benign	Het

Other mutations in Trpm3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Trpm3	APN	19	22,987,659 (GRCm38)	missense	probably benign	0.00
IGL00773:Trpm3	APN	19	22,900,159 (GRCm38)	missense	possibly damaging	0.92
IGL00852:Trpm3	APN	19	22,987,071 (GRCm38)	missense	possibly damaging	0.93
IGL01597:Trpm3	APN	19	22,715,246 (GRCm38)	missense	probably damaging	1.00
IGL01607:Trpm3	APN	19	22,987,127 (GRCm38)	missense	probably benign	0.01
IGL01818:Trpm3	APN	19	22,914,474 (GRCm38)	missense	probably damaging	1.00
IGL01890:Trpm3	APN	19	22,711,719 (GRCm38)	missense	probably damaging	0.98
IGL02016:Trpm3	APN	19	22,902,069 (GRCm38)	nonsense	probably null
IGL02324:Trpm3	APN	19	22,698,779 (GRCm38)	missense	probably benign	0.25
IGL02947:Trpm3	APN	19	22,901,119 (GRCm38)	missense	probably damaging	0.99
IGL03037:Trpm3	APN	19	22,889,412 (GRCm38)	missense	possibly damaging	0.85
IGL03128:Trpm3	APN	19	22,914,465 (GRCm38)	missense	probably damaging	1.00
IGL03335:Trpm3	APN	19	22,926,071 (GRCm38)	critical splice donor site	probably null
IGL03354:Trpm3	APN	19	22,856,718 (GRCm38)	missense	probably damaging	1.00
bit	UTSW	19	22,987,869 (GRCm38)	missense	probably benign	0.00
G1patch:Trpm3	UTSW	19	22,926,028 (GRCm38)	missense	probably damaging	1.00
P0041:Trpm3	UTSW	19	22,897,686 (GRCm38)	missense	probably benign	0.01
R0001:Trpm3	UTSW	19	22,715,331 (GRCm38)	missense	possibly damaging	0.70
R0007:Trpm3	UTSW	19	22,987,529 (GRCm38)	missense	probably benign	0.00
R0007:Trpm3	UTSW	19	22,987,529 (GRCm38)	missense	probably benign	0.00
R0009:Trpm3	UTSW	19	22,914,446 (GRCm38)	missense	probably damaging	1.00
R0009:Trpm3	UTSW	19	22,914,446 (GRCm38)	missense	probably damaging	1.00
R0142:Trpm3	UTSW	19	22,987,916 (GRCm38)	missense	probably damaging	0.98
R0194:Trpm3	UTSW	19	22,715,356 (GRCm38)	splice site	probably null
R0268:Trpm3	UTSW	19	22,897,521 (GRCm38)	critical splice donor site	probably null
R0299:Trpm3	UTSW	19	22,986,873 (GRCm38)	missense	possibly damaging	0.62
R0449:Trpm3	UTSW	19	22,988,054 (GRCm38)	missense	probably benign
R0481:Trpm3	UTSW	19	22,901,071 (GRCm38)	missense	possibly damaging	0.51
R0496:Trpm3	UTSW	19	22,698,778 (GRCm38)	missense	probably benign	0.00
R0499:Trpm3	UTSW	19	22,986,873 (GRCm38)	missense	possibly damaging	0.62
R0550:Trpm3	UTSW	19	22,987,812 (GRCm38)	missense	probably damaging	0.97
R0729:Trpm3	UTSW	19	22,987,789 (GRCm38)	missense	probably benign
R0883:Trpm3	UTSW	19	22,978,654 (GRCm38)	missense	probably damaging	1.00
R0926:Trpm3	UTSW	19	22,988,043 (GRCm38)	missense	probably benign	0.02
R1185:Trpm3	UTSW	19	22,914,417 (GRCm38)	splice site	probably benign
R1185:Trpm3	UTSW	19	22,914,417 (GRCm38)	splice site	probably benign
R1513:Trpm3	UTSW	19	22,986,872 (GRCm38)	missense	possibly damaging	0.96
R1521:Trpm3	UTSW	19	22,901,221 (GRCm38)	missense	probably damaging	1.00
R1522:Trpm3	UTSW	19	22,978,334 (GRCm38)	missense	probably benign	0.39
R1569:Trpm3	UTSW	19	22,889,445 (GRCm38)	critical splice donor site	probably null
R1598:Trpm3	UTSW	19	22,733,024 (GRCm38)	missense	possibly damaging	0.47
R1600:Trpm3	UTSW	19	22,139,155 (GRCm38)	missense	probably benign	0.00
R1616:Trpm3	UTSW	19	22,982,712 (GRCm38)	missense	probably damaging	1.00
R1619:Trpm3	UTSW	19	22,711,907 (GRCm38)	missense	probably damaging	0.99
R1923:Trpm3	UTSW	19	22,885,412 (GRCm38)	missense	probably damaging	1.00
R1985:Trpm3	UTSW	19	22,926,082 (GRCm38)	missense	possibly damaging	0.56
R2002:Trpm3	UTSW	19	22,982,583 (GRCm38)	missense	probably damaging	1.00
R2249:Trpm3	UTSW	19	22,733,034 (GRCm38)	missense	probably benign	0.15
R3719:Trpm3	UTSW	19	22,986,990 (GRCm38)	missense	possibly damaging	0.95
R3766:Trpm3	UTSW	19	22,448,377 (GRCm38)	missense	probably benign
R3774:Trpm3	UTSW	19	22,987,975 (GRCm38)	missense	probably benign	0.03
R3774:Trpm3	UTSW	19	22,978,602 (GRCm38)	missense	possibly damaging	0.66
R3776:Trpm3	UTSW	19	22,978,602 (GRCm38)	missense	possibly damaging	0.66
R3820:Trpm3	UTSW	19	22,987,449 (GRCm38)	missense	probably benign	0.00
R3899:Trpm3	UTSW	19	22,901,160 (GRCm38)	missense	possibly damaging	0.90
R4204:Trpm3	UTSW	19	22,987,564 (GRCm38)	missense	probably benign	0.00
R4238:Trpm3	UTSW	19	22,978,638 (GRCm38)	missense	probably damaging	1.00
R4301:Trpm3	UTSW	19	22,987,292 (GRCm38)	missense	probably benign	0.23
R4344:Trpm3	UTSW	19	22,897,697 (GRCm38)	missense	probably damaging	0.99
R4345:Trpm3	UTSW	19	22,897,697 (GRCm38)	missense	probably damaging	0.99
R4365:Trpm3	UTSW	19	22,978,330 (GRCm38)	missense	probably benign	0.00
R4510:Trpm3	UTSW	19	22,988,017 (GRCm38)	missense	probably benign	0.00
R4511:Trpm3	UTSW	19	22,988,017 (GRCm38)	missense	probably benign	0.00
R4565:Trpm3	UTSW	19	22,987,869 (GRCm38)	missense	probably benign	0.00
R4573:Trpm3	UTSW	19	22,902,142 (GRCm38)	missense	probably damaging	1.00
R4606:Trpm3	UTSW	19	22,978,624 (GRCm38)	missense	probably benign	0.26
R4677:Trpm3	UTSW	19	22,987,388 (GRCm38)	missense	possibly damaging	0.95
R4684:Trpm3	UTSW	19	22,987,781 (GRCm38)	missense	probably benign
R4713:Trpm3	UTSW	19	22,889,435 (GRCm38)	missense	possibly damaging	0.83
R4745:Trpm3	UTSW	19	22,715,295 (GRCm38)	missense	possibly damaging	0.67
R5015:Trpm3	UTSW	19	22,711,712 (GRCm38)	missense	probably damaging	1.00
R5030:Trpm3	UTSW	19	22,698,766 (GRCm38)	missense	probably benign	0.01
R5074:Trpm3	UTSW	19	22,885,349 (GRCm38)	missense	possibly damaging	0.65
R5089:Trpm3	UTSW	19	22,766,756 (GRCm38)	missense	probably damaging	0.97
R5100:Trpm3	UTSW	19	22,918,766 (GRCm38)	missense	probably damaging	0.99
R5108:Trpm3	UTSW	19	22,904,714 (GRCm38)	missense	probably benign	0.06
R5204:Trpm3	UTSW	19	22,448,341 (GRCm38)	nonsense	probably null
R5213:Trpm3	UTSW	19	22,697,454 (GRCm38)	nonsense	probably null
R5358:Trpm3	UTSW	19	22,925,968 (GRCm38)	missense	probably damaging	1.00
R5374:Trpm3	UTSW	19	22,926,184 (GRCm38)	nonsense	probably null
R5382:Trpm3	UTSW	19	22,885,341 (GRCm38)	splice site	probably null
R5509:Trpm3	UTSW	19	22,987,258 (GRCm38)	missense	probably damaging	0.99
R5558:Trpm3	UTSW	19	22,978,573 (GRCm38)	missense	probably damaging	1.00
R6154:Trpm3	UTSW	19	22,987,814 (GRCm38)	missense	probably damaging	1.00
R6250:Trpm3	UTSW	19	22,910,054 (GRCm38)	missense	probably benign	0.01
R6433:Trpm3	UTSW	19	22,901,305 (GRCm38)	missense	probably damaging	1.00
R6542:Trpm3	UTSW	19	22,926,113 (GRCm38)	missense	probably benign	0.04
R6630:Trpm3	UTSW	19	22,987,983 (GRCm38)	missense	probably benign	0.00
R6640:Trpm3	UTSW	19	22,978,582 (GRCm38)	missense	probably damaging	1.00
R6725:Trpm3	UTSW	19	22,926,028 (GRCm38)	missense	probably damaging	1.00
R7275:Trpm3	UTSW	19	22,978,684 (GRCm38)	missense	possibly damaging	0.71
R7371:Trpm3	UTSW	19	22,902,193 (GRCm38)	missense	probably benign	0.27
R7467:Trpm3	UTSW	19	22,978,334 (GRCm38)	missense	possibly damaging	0.82
R7488:Trpm3	UTSW	19	22,978,573 (GRCm38)	missense	probably damaging	1.00
R7495:Trpm3	UTSW	19	22,897,796 (GRCm38)	missense	probably benign	0.28
R7600:Trpm3	UTSW	19	22,926,094 (GRCm38)	missense	possibly damaging	0.68
R7710:Trpm3	UTSW	19	22,918,790 (GRCm38)	missense	probably damaging	0.97
R7877:Trpm3	UTSW	19	22,904,784 (GRCm38)	missense	probably benign	0.25
R8184:Trpm3	UTSW	19	22,918,696 (GRCm38)	missense	possibly damaging	0.46
R8234:Trpm3	UTSW	19	22,715,276 (GRCm38)	missense	possibly damaging	0.47
R8236:Trpm3	UTSW	19	22,987,408 (GRCm38)	missense	probably benign	0.00
R8443:Trpm3	UTSW	19	22,698,862 (GRCm38)	missense	possibly damaging	0.90
R8470:Trpm3	UTSW	19	22,910,137 (GRCm38)	missense	possibly damaging	0.91
R8784:Trpm3	UTSW	19	22,918,676 (GRCm38)	missense	probably benign	0.07
R8816:Trpm3	UTSW	19	22,988,216 (GRCm38)	missense	probably damaging	0.97
R8818:Trpm3	UTSW	19	22,978,588 (GRCm38)	missense	possibly damaging	0.81
R8875:Trpm3	UTSW	19	22,910,129 (GRCm38)	missense	probably damaging	1.00
R8931:Trpm3	UTSW	19	22,766,670 (GRCm38)	missense	probably damaging	1.00
R8969:Trpm3	UTSW	19	22,925,944 (GRCm38)	missense	probably damaging	0.98
R8987:Trpm3	UTSW	19	22,918,760 (GRCm38)	missense	probably damaging	1.00
R9300:Trpm3	UTSW	19	22,978,381 (GRCm38)	missense	possibly damaging	0.49
R9327:Trpm3	UTSW	19	22,918,640 (GRCm38)	missense	possibly damaging	0.56
R9354:Trpm3	UTSW	19	22,448,332 (GRCm38)	missense	probably benign
R9514:Trpm3	UTSW	19	22,982,676 (GRCm38)	missense	probably benign	0.42
R9545:Trpm3	UTSW	19	22,901,094 (GRCm38)	missense	probably benign	0.24
R9712:Trpm3	UTSW	19	22,715,352 (GRCm38)	missense	possibly damaging	0.55
R9750:Trpm3	UTSW	19	22,926,131 (GRCm38)	missense	probably benign	0.00
Z1176:Trpm3	UTSW	19	22,987,490 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TAGACCTTCAGACACATCTTCTTG -3'
(R):5'- GCATAACATCACTCTGCGGTG -3'

Sequencing Primer
(F):5'- GATCCAAAGTTCAATCAGTCCTGG -3'
(R):5'- ATCACTCTGCGGTGGAGGG -3'

Posted On

2022-10-06