Mutagenetix > Incidental Mutation

Incidental Mutation 'R9723:Cyp2b9'

730921

Institutional Source

Beutler Lab

Gene Symbol

Cyp2b9

Ensembl Gene

ENSMUSG00000040660

Gene Name

cytochrome P450, family 2, subfamily b, polypeptide 9

Synonyms

16\alphaoh-a, phenobarbitol inducible, type a, Cyp2b

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.098) question?

Stock #

R9723 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

26173411-26210661 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 26210171 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 455 (Q455*)

Ref Sequence

ENSEMBL: ENSMUSP00000080846 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082214]

AlphaFold

P12790

Predicted Effect

probably null
Transcript: ENSMUST00000082214
AA Change: Q455*

SMART Domains

Protein: ENSMUSP00000080846
Gene: ENSMUSG00000040660
AA Change: Q455*

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:p450	31	488	1.7e-146	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930430A15Rik	T	C	2: 111,228,355	H84R	probably damaging	Het
Abcb6	G	A	1: 75,179,722	R133W	probably benign	Het
Abcc3	T	A	11: 94,359,899	T877S	probably benign	Het
Alb	A	T	5: 90,464,103	K130N	probably damaging	Het
Alkbh8	G	A	9: 3,385,283	S560N	probably benign	Het
Angel2	T	G	1: 190,939,145	L234R	probably damaging	Het
Catip	C	T	1: 74,364,586	T154I	probably benign	Het
Ccdc163	C	T	4: 116,712,398	Q102*	probably null	Het
Cep250	A	G	2: 155,981,417	E997G	probably benign	Het
Ces2f	T	C	8: 104,950,831	I183T	possibly damaging	Het
Commd8	A	G	5: 72,160,966	V158A	possibly damaging	Het
D5Ertd579e	G	T	5: 36,614,940	H704N	probably damaging	Het
Dnah1	T	C	14: 31,265,989	T3491A	probably damaging	Het
Dnaic1	T	G	4: 41,603,302	F195C	possibly damaging	Het
Dock7	A	G	4: 99,020,033	V620A		Het
Dock7	A	T	4: 99,072,423	D289E		Het
Dpys	T	A	15: 39,828,113	E271V	probably damaging	Het
Elovl3	T	C	19: 46,134,716	Y244H	probably damaging	Het
Enam	G	A	5: 88,504,382	G1250E	probably damaging	Het
Espl1	C	T	15: 102,320,735	T1774M	probably benign	Het
Fasl	T	C	1: 161,787,966	K107R	probably benign	Het
Fbn1	A	T	2: 125,360,199	C1251*	probably null	Het
Fn1	G	T	1: 71,624,210	Q1040K	possibly damaging	Het
Frem3	C	T	8: 80,614,723	S1215L	probably benign	Het
G3bp2	A	T	5: 92,066,529	D135E	possibly damaging	Het
Gabpb1	C	T	2: 126,646,728	V240I	probably benign	Het
Galnt12	C	G	4: 47,119,541	Y452*	probably null	Het
Ggta1	G	T	2: 35,413,406	D91E	probably benign	Het
Gm12258	C	T	11: 58,859,622	P541L	unknown	Het
Gm12789	T	A	4: 101,988,886	W115R	possibly damaging	Het
Gm8126	T	C	14: 43,261,684		probably null	Het
Gpr179	A	T	11: 97,334,720	L2203H	possibly damaging	Het
Hapln3	A	T	7: 79,121,988	V51E	possibly damaging	Het
Hist1h1t	A	G	13: 23,695,923	K20E	probably damaging	Het
Ifit1	A	G	19: 34,648,857	*464W	probably null	Het
Il1r1	A	C	1: 40,293,561	I137L	probably benign	Het
Il1rap	T	A	16: 26,624,157	M1K	probably null	Het
Lgi2	A	G	5: 52,538,501	L372P	probably damaging	Het
Lrrc46	A	G	11: 97,034,947	S230P	possibly damaging	Het
Ltbr	G	A	6: 125,307,385	R365W	probably damaging	Het
Mapk8ip3	C	A	17: 24,913,611	W339L	possibly damaging	Het
Msln	A	T	17: 25,750,034	M459K	possibly damaging	Het
Msr1	A	G	8: 39,589,316	V406A	possibly damaging	Het
Ndst2	G	T	14: 20,725,444	D659E	probably benign	Het
Npat	T	A	9: 53,562,446	S513T	probably benign	Het
Npat	T	G	9: 53,570,561	L1190V	probably damaging	Het
Npc1	A	G	18: 12,210,592	I448T	probably benign	Het
Omd	T	C	13: 49,590,362	F296S	probably damaging	Het
Oprm1	A	G	10: 6,838,514	N423S	possibly damaging	Het
Oxtr	G	A	6: 112,489,343	T152I	probably benign	Het
Pak2	A	T	16: 32,033,832	V297E	probably damaging	Het
Pcnx4	T	C	12: 72,556,265	Y434H	probably damaging	Het
Pet100	G	A	8: 3,622,374	M20I	probably damaging	Het
Pfpl	G	C	19: 12,428,933	E183Q	probably damaging	Het
Plekhn1	A	G	4: 156,222,418	S505P	probably benign	Het
Plin5	G	A	17: 56,116,290	A90V	probably damaging	Het
Poc5	A	G	13: 96,414,518	T526A	probably benign	Het
Pramel7	C	T	2: 87,489,675	V425I	possibly damaging	Het
Rad51d	A	T	11: 82,890,336		probably null	Het
Rbp3	T	C	14: 33,955,517	M474T	possibly damaging	Het
Rcan3	A	G	4: 135,425,369	S14P	probably benign	Het
Rps6ka4	A	T	19: 6,839,295	V140E	probably damaging	Het
Rsbn1l	G	A	5: 20,896,466	S692L	possibly damaging	Het
Sgk1	A	T	10: 21,996,340	I272F	probably damaging	Het
Skor1	A	G	9: 63,146,432	V85A	probably damaging	Het
Slc12a1	A	T	2: 125,217,907	D909V	probably damaging	Het
Slc25a18	C	A	6: 120,793,528	A283E	probably benign	Het
Slc41a1	T	C	1: 131,844,365	L411P	possibly damaging	Het
Spata13	T	C	14: 60,691,049	S19P	probably damaging	Het
Stab1	T	A	14: 31,163,891	H42L	probably benign	Het
Stag3	T	A	5: 138,299,841	D698E	probably benign	Het
Syngap1	G	A	17: 26,970,536	R1305H	possibly damaging	Het
Tek	T	G	4: 94,804,302	W216G	possibly damaging	Het
Trappc9	A	G	15: 72,590,114	Y1101H	possibly damaging	Het
Ttn	A	G	2: 76,771,585	Y10251H	probably damaging	Het
Uhrf1	A	G	17: 56,318,061	K535E	probably damaging	Het
Usp14	G	T	18: 10,009,993	Q185K	probably damaging	Het
Usp32	A	T	11: 85,044,710	Y413*	probably null	Het
Uvssa	G	A	5: 33,390,038		probably null	Het
Vmn1r177	A	T	7: 23,866,349	L34Q	probably damaging	Het
Wdr41	T	C	13: 95,015,163	L277P	probably damaging	Het
Zc2hc1b	T	A	10: 13,168,753	L55F	probably damaging	Het
Zfp345	A	C	2: 150,472,269	Y449*	probably null	Het

Other mutations in Cyp2b9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Cyp2b9	APN	7	26198505	missense	probably damaging	0.99
IGL01133:Cyp2b9	APN	7	26210235	missense	probably damaging	1.00
IGL01331:Cyp2b9	APN	7	26187715	missense	probably damaging	0.99
IGL02281:Cyp2b9	APN	7	26201104	missense	probably damaging	0.99
IGL02502:Cyp2b9	APN	7	26187814	critical splice donor site	probably null
IGL02713:Cyp2b9	APN	7	26173520	missense	probably benign	0.12
IGL03032:Cyp2b9	APN	7	26198600	splice site	probably benign
IGL03307:Cyp2b9	APN	7	26199051	missense	probably benign	0.00
R0010:Cyp2b9	UTSW	7	26186753	splice site	probably benign
R0025:Cyp2b9	UTSW	7	26200813	missense	probably benign	0.09
R0040:Cyp2b9	UTSW	7	26173474	missense	possibly damaging	0.68
R0184:Cyp2b9	UTSW	7	26187007	nonsense	probably null
R0370:Cyp2b9	UTSW	7	26210106	missense	probably damaging	1.00
R1595:Cyp2b9	UTSW	7	26200907	missense	possibly damaging	0.72
R1751:Cyp2b9	UTSW	7	26186675	missense	probably benign	0.05
R1835:Cyp2b9	UTSW	7	26200783	missense	probably benign
R1879:Cyp2b9	UTSW	7	26198569	missense	probably damaging	0.99
R2256:Cyp2b9	UTSW	7	26173605	critical splice donor site	probably null
R2257:Cyp2b9	UTSW	7	26173605	critical splice donor site	probably null
R2418:Cyp2b9	UTSW	7	26186707	missense	probably benign	0.00
R3420:Cyp2b9	UTSW	7	26210103	missense	probably damaging	1.00
R4088:Cyp2b9	UTSW	7	26173456	missense	probably damaging	0.99
R4412:Cyp2b9	UTSW	7	26198443	missense	probably damaging	1.00
R4495:Cyp2b9	UTSW	7	26200755	missense	probably benign	0.00
R4615:Cyp2b9	UTSW	7	26201125	missense	probably damaging	1.00
R5375:Cyp2b9	UTSW	7	26187742	missense	probably damaging	1.00
R5426:Cyp2b9	UTSW	7	26187655	missense	probably benign
R5862:Cyp2b9	UTSW	7	26187807	missense	probably benign	0.01
R6237:Cyp2b9	UTSW	7	26173574	missense	probably benign	0.02
R6445:Cyp2b9	UTSW	7	26186987	missense	probably benign	0.13
R6992:Cyp2b9	UTSW	7	26201139	missense	probably benign	0.00
R7515:Cyp2b9	UTSW	7	26199171	missense	probably damaging	1.00
R7654:Cyp2b9	UTSW	7	26186942	missense	possibly damaging	0.72
R7816:Cyp2b9	UTSW	7	26201092	missense	probably benign	0.01
R7850:Cyp2b9	UTSW	7	26186686	nonsense	probably null
R8734:Cyp2b9	UTSW	7	26198610	intron	probably benign
R8790:Cyp2b9	UTSW	7	26198742	intron	probably benign
R8839:Cyp2b9	UTSW	7	26200760	missense	probably damaging	0.96
R9209:Cyp2b9	UTSW	7	26173579	missense	possibly damaging	0.52
R9787:Cyp2b9	UTSW	7	26200834	missense	probably benign	0.04
Z1177:Cyp2b9	UTSW	7	26201163	missense	probably benign	0.31

Predicted Primers

PCR Primer
(F):5'- AGTGCATTGGACAGGGATGTC -3'
(R):5'- TTGGAGCTTTGGAGAACAACAG -3'

Sequencing Primer
(F):5'- TGCATTGGACAGGGATGTCTAAATG -3'
(R):5'- TGTGAGCAGCTACCAATGGC -3'

Posted On

2022-10-06