Mutagenetix > Incidental Mutation

Incidental Mutation 'R9737:Vnn3'

731704

Institutional Source

Beutler Lab

Gene Symbol

Vnn3

Ensembl Gene

ENSMUSG00000020010

Gene Name

vanin 3

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R9737 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23727360-23745741 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 23741813 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 373 (M373V)

Ref Sequence

ENSEMBL: ENSMUSP00000020190 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020190]

AlphaFold

Q9QZ25

Predicted Effect

probably benign
Transcript: ENSMUST00000020190
AA Change: M373V

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000020190
Gene: ENSMUSG00000020010
AA Change: M373V

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:CN_hydrolase	51	296	6.9e-19	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 99.4%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 18 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adnp	T	C	2: 168,026,918 (GRCm39)	K126E	possibly damaging	Het
Bnip3l	G	A	14: 67,246,214 (GRCm39)	P7L	possibly damaging	Het
Ccnjl	C	A	11: 43,476,166 (GRCm39)	T263K	probably benign	Het
Cfhr4	A	T	1: 139,708,872 (GRCm39)	I12N	probably damaging	Het
D6Ertd527e	G	C	6: 87,088,839 (GRCm39)	S334T	unknown	Het
Epha2	A	G	4: 141,045,814 (GRCm39)	E446G	probably benign	Het
Gtf2ird1	A	G	5: 134,408,794 (GRCm39)	Y702H	probably damaging	Het
Igkv10-96	T	C	6: 68,608,957 (GRCm39)	T113A	probably benign	Het
Jhy	T	C	9: 40,808,748 (GRCm39)	E671G	probably damaging	Het
Ngdn	A	G	14: 55,259,339 (GRCm39)	K161R	possibly damaging	Het
Pabpc4l	A	G	3: 46,401,267 (GRCm39)	S126P	probably damaging	Het
Phlpp2	G	A	8: 110,663,714 (GRCm39)	D918N	probably damaging	Het
Pkd2	A	T	5: 104,651,349 (GRCm39)	H899L	possibly damaging	Het
Pou6f1	A	G	15: 100,481,282 (GRCm39)	S301P	probably benign	Het
Sidt1	A	G	16: 44,102,243 (GRCm39)	Y306H	probably damaging	Het
Trav13-1	A	T	14: 53,782,510 (GRCm39)	T14S	probably benign	Het
Vmn2r99	A	C	17: 19,582,563 (GRCm39)	E56A	probably benign	Het
Zkscan5	A	G	5: 145,142,136 (GRCm39)	H11R	probably benign	Het

Other mutations in Vnn3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00675:Vnn3	APN	10	23,743,066 (GRCm39)	missense	possibly damaging	0.77
IGL01300:Vnn3	APN	10	23,740,263 (GRCm39)	missense	possibly damaging	0.87
IGL01349:Vnn3	APN	10	23,727,814 (GRCm39)	missense	probably damaging	0.99
IGL02491:Vnn3	APN	10	23,741,816 (GRCm39)	missense	probably benign	0.11
IGL03256:Vnn3	APN	10	23,727,698 (GRCm39)	splice site	probably benign
IGL03289:Vnn3	APN	10	23,741,735 (GRCm39)	missense	possibly damaging	0.92
IGL02799:Vnn3	UTSW	10	23,727,869 (GRCm39)	missense	possibly damaging	0.64
R0599:Vnn3	UTSW	10	23,741,603 (GRCm39)	missense	possibly damaging	0.94
R1703:Vnn3	UTSW	10	23,741,828 (GRCm39)	missense	probably benign
R1753:Vnn3	UTSW	10	23,741,718 (GRCm39)	missense	probably benign	0.27
R2119:Vnn3	UTSW	10	23,740,311 (GRCm39)	missense	probably damaging	1.00
R2288:Vnn3	UTSW	10	23,740,354 (GRCm39)	missense	probably benign	0.03
R4255:Vnn3	UTSW	10	23,741,720 (GRCm39)	missense	probably benign	0.18
R4458:Vnn3	UTSW	10	23,741,567 (GRCm39)	missense	probably benign	0.23
R4518:Vnn3	UTSW	10	23,743,124 (GRCm39)	missense	possibly damaging	0.77
R4545:Vnn3	UTSW	10	23,732,224 (GRCm39)	missense	probably benign	0.00
R4723:Vnn3	UTSW	10	23,727,589 (GRCm39)	missense	possibly damaging	0.88
R4791:Vnn3	UTSW	10	23,740,519 (GRCm39)	missense	probably benign
R4921:Vnn3	UTSW	10	23,740,473 (GRCm39)	missense	probably benign	0.01
R5152:Vnn3	UTSW	10	23,740,237 (GRCm39)	missense	probably benign	0.01
R5390:Vnn3	UTSW	10	23,727,483 (GRCm39)	start codon destroyed	probably null	1.00
R5545:Vnn3	UTSW	10	23,742,992 (GRCm39)	missense	probably benign	0.00
R6197:Vnn3	UTSW	10	23,732,187 (GRCm39)	missense	probably damaging	1.00
R6751:Vnn3	UTSW	10	23,745,523 (GRCm39)	missense	probably benign	0.00
R6846:Vnn3	UTSW	10	23,727,620 (GRCm39)	missense	probably benign
R6917:Vnn3	UTSW	10	23,741,832 (GRCm39)	missense	possibly damaging	0.50
R7073:Vnn3	UTSW	10	23,740,311 (GRCm39)	missense	probably damaging	1.00
R7100:Vnn3	UTSW	10	23,741,840 (GRCm39)	missense	probably damaging	1.00
R7152:Vnn3	UTSW	10	23,727,513 (GRCm39)	missense	possibly damaging	0.88
R7336:Vnn3	UTSW	10	23,727,806 (GRCm39)	missense	probably benign	0.42
R7421:Vnn3	UTSW	10	23,741,666 (GRCm39)	missense	probably benign	0.06
R7875:Vnn3	UTSW	10	23,743,146 (GRCm39)	missense	possibly damaging	0.80
R8353:Vnn3	UTSW	10	23,745,443 (GRCm39)	missense	probably benign	0.01
R8453:Vnn3	UTSW	10	23,745,443 (GRCm39)	missense	probably benign	0.01
R8465:Vnn3	UTSW	10	23,741,780 (GRCm39)	missense	possibly damaging	0.67
R9105:Vnn3	UTSW	10	23,740,461 (GRCm39)	missense	probably damaging	1.00
R9261:Vnn3	UTSW	10	23,741,607 (GRCm39)	missense	probably damaging	0.97
R9441:Vnn3	UTSW	10	23,740,498 (GRCm39)	missense	possibly damaging	0.94
R9718:Vnn3	UTSW	10	23,745,454 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GAGGTTAACTGGAGCGCTTATG -3'
(R):5'- GTTTAACGAAAAGCTAGGATACCC -3'

Sequencing Primer
(F):5'- TTATGCCAGGACTGTGAAGCC -3'
(R):5'- CCATCAGATTGTAGGCAAGTCTG -3'

Posted On

2022-11-14