Mutagenetix > Incidental Mutation

Incidental Mutation 'R9741:Vmn2r59'

731832

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R9741 (G1)

Quality Score

215.009

Status

Not validated

Chromosome

Chromosomal Location

41661216-41708405 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 41708209 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 66 (D66V)

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

probably damaging
Transcript: ENSMUST00000168489
AA Change: D66V

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032
AA Change: D66V

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.5%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd6	T	A	4: 32,860,339 (GRCm39)	K35*	probably null	Het
Apoc3	G	T	9: 46,145,998 (GRCm39)	T5K	unknown	Het
Arhgap20	T	A	9: 51,760,730 (GRCm39)	Y860*	probably null	Het
Arhgef11	T	C	3: 87,595,156 (GRCm39)	S123P	probably benign	Het
Armh4	A	G	14: 50,008,081 (GRCm39)	I464T	probably benign	Het
Axdnd1	T	A	1: 156,169,385 (GRCm39)	Y827F	probably benign	Het
Bbs5	A	G	2: 69,484,695 (GRCm39)	T122A	probably benign	Het
Btbd6	T	A	12: 112,940,923 (GRCm39)	D173E	probably benign	Het
Cblb	T	C	16: 51,932,490 (GRCm39)	I149T	probably damaging	Het
Cyp2ab1	G	A	16: 20,132,953 (GRCm39)	R214C	probably damaging	Het
Dbn1	C	T	13: 55,624,114 (GRCm39)	S372N	possibly damaging	Het
Dlg1	T	A	16: 31,676,735 (GRCm39)	Y776*	probably null	Het
Dock9	A	T	14: 121,877,516 (GRCm39)	I409N	probably damaging	Het
Ect2	A	T	3: 27,156,607 (GRCm39)	N815K	probably benign	Het
Fbxw11	G	T	11: 32,685,358 (GRCm39)	V398L	probably damaging	Het
Gcsam	T	C	16: 45,436,319 (GRCm39)	F34S	possibly damaging	Het
Ghsr	G	A	3: 27,428,898 (GRCm39)	V308I	possibly damaging	Het
Gm40460	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 141,794,450 (GRCm39)		probably benign	Het
H1f10	A	G	6: 87,958,200 (GRCm39)	Y47H	probably damaging	Het
Hspg2	T	C	4: 137,239,962 (GRCm39)	F510S	probably damaging	Het
Htr1a	T	C	13: 105,581,861 (GRCm39)	V367A	possibly damaging	Het
Iars1	T	G	13: 49,844,978 (GRCm39)	F163C	probably damaging	Het
Igfn1	T	C	1: 135,895,383 (GRCm39)	T1728A	probably benign	Het
Ighv7-3	C	T	12: 114,116,995 (GRCm39)	V56I	probably benign	Het
Lrp1b	A	T	2: 41,002,300 (GRCm39)	I2133N		Het
Lrrc37	T	C	11: 103,504,255 (GRCm39)	E2571G	possibly damaging	Het
Or2d2	A	G	7: 106,728,366 (GRCm39)	V78A	possibly damaging	Het
Or52z1	G	T	7: 103,436,941 (GRCm39)	P181H	probably benign	Het
Pkd1l1	T	C	11: 8,897,224 (GRCm39)	T562A		Het
Ppp1r10	G	A	17: 36,237,331 (GRCm39)	R167Q	possibly damaging	Het
Ppp1r13l	C	T	7: 19,103,725 (GRCm39)	R69W	probably damaging	Het
Proser2	G	A	2: 6,105,580 (GRCm39)	A328V	probably benign	Het
Rhou	A	G	8: 124,380,914 (GRCm39)	Y77C	possibly damaging	Het
Rprml	T	A	11: 103,540,857 (GRCm39)	L84Q	probably damaging	Het
Ryr3	C	T	2: 112,477,271 (GRCm39)	C4515Y	probably benign	Het
Slc9a3	T	A	13: 74,306,994 (GRCm39)	I373N	possibly damaging	Het
Snx21	C	T	2: 164,634,231 (GRCm39)	A339V	probably benign	Het
Srpk1	G	A	17: 28,818,652 (GRCm39)	P395S	probably benign	Het
Syt14	A	T	1: 192,666,449 (GRCm39)	S152T	unknown	Het
Urb2	G	T	8: 124,755,751 (GRCm39)	R486L	probably damaging	Het
Utrn	T	A	10: 12,702,564 (GRCm39)	M7L	probably benign	Het
Znrf2	T	C	6: 54,855,370 (GRCm39)	I197T	probably damaging	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	41,661,488 (GRCm39)	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	41,661,983 (GRCm39)	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	41,695,593 (GRCm39)	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	41,661,817 (GRCm39)	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	41,661,655 (GRCm39)	missense	probably benign
IGL03346:Vmn2r59	APN	7	41,693,253 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	41,708,340 (GRCm39)	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	41,661,862 (GRCm39)	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	41,695,205 (GRCm39)	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	41,695,725 (GRCm39)	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	41,661,722 (GRCm39)	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	41,696,432 (GRCm39)	nonsense	probably null
R0370:Vmn2r59	UTSW	7	41,662,150 (GRCm39)	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	41,695,916 (GRCm39)	splice site	probably benign
R0465:Vmn2r59	UTSW	7	41,696,332 (GRCm39)	missense	probably benign
R0487:Vmn2r59	UTSW	7	41,696,528 (GRCm39)	nonsense	probably null
R0576:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	41,708,308 (GRCm39)	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	41,661,218 (GRCm39)	makesense	probably null
R1387:Vmn2r59	UTSW	7	41,695,521 (GRCm39)	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	41,695,133 (GRCm39)	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	41,695,629 (GRCm39)	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	41,695,251 (GRCm39)	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	41,693,203 (GRCm39)	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	41,708,326 (GRCm39)	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2257:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2441:Vmn2r59	UTSW	7	41,695,570 (GRCm39)	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	41,693,190 (GRCm39)	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	41,661,370 (GRCm39)	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	41,695,744 (GRCm39)	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	41,670,732 (GRCm39)	intron	probably benign
R4357:Vmn2r59	UTSW	7	41,661,644 (GRCm39)	missense	probably damaging	1.00
R4445:Vmn2r59	UTSW	7	41,691,874 (GRCm39)	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	41,695,497 (GRCm39)	missense	possibly damaging	0.93
R4587:Vmn2r59	UTSW	7	41,695,648 (GRCm39)	missense	probably benign	0.00
R4616:Vmn2r59	UTSW	7	41,661,862 (GRCm39)	missense	probably benign
R4653:Vmn2r59	UTSW	7	41,693,228 (GRCm39)	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	41,661,686 (GRCm39)	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	41,695,218 (GRCm39)	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	41,693,077 (GRCm39)	missense	probably benign
R5045:Vmn2r59	UTSW	7	41,695,496 (GRCm39)	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	41,691,834 (GRCm39)	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	41,696,247 (GRCm39)	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	41,695,105 (GRCm39)	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	41,695,318 (GRCm39)	missense	probably benign	0.27
R5616:Vmn2r59	UTSW	7	41,708,191 (GRCm39)	splice site	probably null
R5625:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	41,695,468 (GRCm39)	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	41,695,491 (GRCm39)	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	41,661,749 (GRCm39)	nonsense	probably null
R6196:Vmn2r59	UTSW	7	41,661,679 (GRCm39)	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	41,691,835 (GRCm39)	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	41,693,177 (GRCm39)	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	41,661,392 (GRCm39)	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	41,693,171 (GRCm39)	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	41,693,277 (GRCm39)	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	41,695,188 (GRCm39)	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	41,695,233 (GRCm39)	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	41,661,443 (GRCm39)	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	41,708,336 (GRCm39)	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	41,695,196 (GRCm39)	nonsense	probably null
R7867:Vmn2r59	UTSW	7	41,661,707 (GRCm39)	missense	probably damaging	1.00
R7975:Vmn2r59	UTSW	7	41,693,199 (GRCm39)	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	41,696,256 (GRCm39)	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	41,661,521 (GRCm39)	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	41,661,247 (GRCm39)	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	41,693,127 (GRCm39)	missense
R9135:Vmn2r59	UTSW	7	41,693,125 (GRCm39)	missense	probably benign	0.33
R9234:Vmn2r59	UTSW	7	41,661,907 (GRCm39)	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	41,695,286 (GRCm39)	nonsense	probably null
R9432:Vmn2r59	UTSW	7	41,696,254 (GRCm39)	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	41,695,590 (GRCm39)	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	41,661,299 (GRCm39)	missense	probably damaging	1.00
R9654:Vmn2r59	UTSW	7	41,693,217 (GRCm39)	missense	probably benign	0.10
X0025:Vmn2r59	UTSW	7	41,695,365 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	41,661,838 (GRCm39)	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	41,691,941 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGGTCCATGCATGACAAACAAAC -3'
(R):5'- CCATCTGTCTCTTGGATGGG -3'

Sequencing Primer
(F):5'- CATGCATGACAAACAAACGATTATGG -3'
(R):5'- TCTCTTGGATGGGCAGATATATATG -3'

Posted On

2022-11-14