Mutagenetix > Incidental Mutation

Incidental Mutation 'R9746:Tgm4'

732134

Institutional Source

Beutler Lab

Gene Symbol

Tgm4

Ensembl Gene

ENSMUSG00000025787

Gene Name

transglutaminase 4 (prostate)

Synonyms

9530008N10Rik, Eapa1, experimental autoimmune prostatitis antigen 1

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R9746 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

123034726-123067561 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 123046569 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 162 (K162N)

Ref Sequence

ENSEMBL: ENSMUSP00000026893 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026893] [ENSMUST00000215247] [ENSMUST00000217607]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000026893
AA Change: K162N

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000026893
Gene: ENSMUSG00000025787
AA Change: K162N

Domain	Start	End	E-Value	Type
Pfam:Transglut_N	8	118	4e-26	PFAM
TGc	247	340	6.25e-42	SMART
Pfam:Transglut_C	573	670	3e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215247

Predicted Effect

probably benign
Transcript: ENSMUST00000217607

Coding Region Coverage

1x: 99.8%
3x: 99.7%
10x: 99.2%
20x: 98.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired copulatory plug formation, reduced fertilization and few litters sired. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 95 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ackr1	T	A	1: 173,332,031 (GRCm38)	H307L	probably benign	Het
Acnat1	A	T	4: 49,450,652 (GRCm38)	L153H	probably damaging	Het
Actn4	A	T	7: 28,919,006 (GRCm38)	D76E	probably benign	Het
Afdn	A	C	17: 13,846,520 (GRCm38)	M640L	probably benign	Het
Angpt1	A	T	15: 42,676,441 (GRCm38)	F7L	probably benign	Het
Ap3b2	A	G	7: 81,476,344 (GRCm38)	F373S	probably damaging	Het
Armc2	T	A	10: 41,924,461 (GRCm38)	Y650F	probably damaging	Het
Atg2b	G	T	12: 105,663,938 (GRCm38)	T398K	possibly damaging	Het
Baz1a	CCATT	CCATTCATT	12: 54,975,110 (GRCm38)		probably null	Het
Capn8	G	A	1: 182,611,105 (GRCm38)		probably null	Het
Cdr1	AAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCC	AAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCC	X: 61,184,524 (GRCm38)		probably benign	Het
Cfap54	T	C	10: 92,801,219 (GRCm38)	N3103D	probably benign	Het
Chd9	G	A	8: 91,011,435 (GRCm38)	R1565Q	unknown	Het
Cir1	G	A	2: 73,303,808 (GRCm38)	T139I	probably damaging	Het
Cmah	T	C	13: 24,435,690 (GRCm38)		probably null	Het
Cnpy1	T	C	5: 28,245,802 (GRCm38)	D2G	probably damaging	Het
Dennd4a	A	T	9: 64,894,511 (GRCm38)	T979S	probably benign	Het
Dnah11	T	A	12: 117,878,576 (GRCm38)	K4423*	probably null	Het
Dnajb4	A	G	3: 152,186,683 (GRCm38)	I171T	possibly damaging	Het
Dpm1	A	T	2: 168,230,387 (GRCm38)		probably null	Het
Dtwd1	A	G	2: 126,154,675 (GRCm38)	T27A	probably benign	Het
Ep400	C	T	5: 110,742,006 (GRCm38)	D464N	unknown	Het
Epb42	A	T	2: 121,024,610 (GRCm38)	I498N	probably benign	Het
Fhod1	A	G	8: 105,337,416 (GRCm38)	V219A	unknown	Het
Gabrb2	A	T	11: 42,626,609 (GRCm38)	E419D	probably benign	Het
Galnt18	A	T	7: 111,471,961 (GRCm38)	N615K	possibly damaging	Het
Gm13084	T	C	4: 143,810,316 (GRCm38)	T482A	probably benign	Het
Gm29106	T	A	1: 118,199,524 (GRCm38)	H315Q	possibly damaging	Het
Gprin2	G	T	14: 34,195,658 (GRCm38)	Q52K	probably benign	Het
Grm4	A	T	17: 27,438,791 (GRCm38)	Y414N	probably damaging	Het
Gulo	C	A	14: 65,988,181 (GRCm38)		probably null	Het
H2-M1	A	C	17: 36,670,105 (GRCm38)	V313G	possibly damaging	Het
Hectd3	T	C	4: 116,995,754 (GRCm38)	W118R	probably damaging	Het
Hs6st3	G	A	14: 119,869,080 (GRCm38)	C300Y	probably damaging	Het
Il1rl2	T	A	1: 40,365,359 (GRCm38)	S547T	possibly damaging	Het
Kank1	G	T	19: 25,409,508 (GRCm38)	V182F	probably damaging	Het
Kif20b	T	A	19: 34,950,749 (GRCm38)	L1137*	probably null	Het
Klhl7	A	G	5: 24,126,820 (GRCm38)		probably null	Het
Krt13	T	A	11: 100,121,161 (GRCm38)	D112V	possibly damaging	Het
Ltbr	A	G	6: 125,313,101 (GRCm38)	V71A	probably benign	Het
Ly6g	T	C	15: 75,158,609 (GRCm38)	V92A	probably benign	Het
Map2k3	G	A	11: 60,932,103 (GRCm38)		probably benign	Het
Mast2	A	C	4: 116,311,730 (GRCm38)	D842E	probably benign	Het
Mpo	T	G	11: 87,803,523 (GRCm38)	M693R	probably benign	Het
Myo15	C	A	11: 60,487,408 (GRCm38)	S212*	probably null	Het
Nadk2	C	T	15: 9,106,736 (GRCm38)	R37*	probably null	Het
Ncapd3	G	A	9: 27,063,359 (GRCm38)	R709H	probably benign	Het
Nck2	T	A	1: 43,533,732 (GRCm38)	Y55*	probably null	Het
Neurl4	A	G	11: 69,907,475 (GRCm38)	D777G	probably damaging	Het
Npr2	G	A	4: 43,633,527 (GRCm38)	V224M	possibly damaging	Het
Nptx2	T	A	5: 144,548,140 (GRCm38)	S148T	probably benign	Het
Nr1d1	T	C	11: 98,770,334 (GRCm38)	T369A	probably benign	Het
Nub1	T	G	5: 24,703,485 (GRCm38)	F411V	probably damaging	Het
Nup188	T	A	2: 30,304,288 (GRCm38)	Y158N	probably damaging	Het
Olfr1031	A	G	2: 85,992,747 (GRCm38)	Y310C	probably benign	Het
Olfr513	A	G	7: 108,755,432 (GRCm38)	N192S	probably benign	Het
Olfr716	A	T	7: 107,147,453 (GRCm38)	I46F	probably benign	Het
Olfr804	A	T	10: 129,705,339 (GRCm38)	I154F	probably damaging	Het
Olfr897-ps1	T	A	9: 38,309,632 (GRCm38)	I279K	unknown	Het
Orc2	C	T	1: 58,497,451 (GRCm38)	G85S	probably damaging	Het
Pak1ip1	T	A	13: 41,009,267 (GRCm38)	V182D	probably damaging	Het
Parvg	T	G	15: 84,326,223 (GRCm38)	C30W	probably benign	Het
Pcdha1	T	A	18: 36,932,660 (GRCm38)	D792E	probably benign	Het
Ppp1r13b	G	T	12: 111,833,808 (GRCm38)	Q635K	probably benign	Het
Psg16	A	G	7: 17,098,161 (GRCm38)	I341V	probably benign	Het
Psme4	C	T	11: 30,876,868 (GRCm38)	Q1796*	probably null	Het
Ptn	T	G	6: 36,715,764 (GRCm38)		probably null	Het
Rapsn	C	T	2: 91,045,478 (GRCm38)	P400L	probably damaging	Het
Rasal1	G	A	5: 120,662,293 (GRCm38)	G207D	probably damaging	Het
Rdx	A	G	9: 52,063,578 (GRCm38)	I5V	probably benign	Het
Rpf1	A	G	3: 146,517,778 (GRCm38)	I105T	probably damaging	Het
Rps15a	A	T	7: 118,109,997 (GRCm38)	F79I	possibly damaging	Het
Rwdd2b	G	A	16: 87,436,753 (GRCm38)	P153L	probably benign	Het
Scaf11	G	A	15: 96,420,417 (GRCm38)	S422L	probably damaging	Het
Serpinb3b	T	A	1: 107,154,673 (GRCm38)	E287V	possibly damaging	Het
Sgta	T	C	10: 81,051,284 (GRCm38)	D49G	possibly damaging	Het
Sin3a	T	A	9: 57,118,074 (GRCm38)	M1068K	probably benign	Het
Slc12a9	G	A	5: 137,321,409 (GRCm38)	R615W	probably damaging	Het
Slc26a3	T	A	12: 31,449,146 (GRCm38)	S151T	probably benign	Het
Slc4a8	C	A	15: 100,783,840 (GRCm38)	H111N	probably damaging	Het
Syngr1	G	A	15: 80,091,458 (GRCm38)	R22K	probably benign	Het
Tars2	A	T	3: 95,754,765 (GRCm38)	V25E	probably benign	Het
Tbx15	A	G	3: 99,352,331 (GRCm38)	Y506C	probably damaging	Het
Tmem253	A	G	14: 52,017,982 (GRCm38)	E83G	probably damaging	Het
Trp73	G	A	4: 154,081,402 (GRCm38)	T118I	probably damaging	Het
Trpm7	A	G	2: 126,822,658 (GRCm38)	S934P	possibly damaging	Het
Ttc23l	G	A	15: 10,523,643 (GRCm38)	S330L	probably benign	Het
Tub	A	C	7: 109,025,638 (GRCm38)	D199A	probably benign	Het
Uri1	A	C	7: 37,996,685 (GRCm38)		probably null	Het
Usp16	C	T	16: 87,479,232 (GRCm38)	A486V	probably benign	Het
Vmn1r12	A	G	6: 57,159,541 (GRCm38)	I208V	probably benign	Het
Vmn2r116	A	T	17: 23,401,823 (GRCm38)	M844L	probably benign	Het
Vmn2r13	C	T	5: 109,191,907 (GRCm38)		probably null	Het
Xpnpep1	T	C	19: 53,013,461 (GRCm38)	D118G	probably damaging	Het
Zfp111	G	A	7: 24,198,642 (GRCm38)	P516S	possibly damaging	Het

Other mutations in Tgm4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00432:Tgm4	APN	9	123,062,382 (GRCm38)	unclassified	probably benign
IGL01402:Tgm4	APN	9	123,051,454 (GRCm38)	missense	possibly damaging	0.82
IGL02000:Tgm4	APN	9	123,056,466 (GRCm38)	missense	probably damaging	1.00
IGL02120:Tgm4	APN	9	123,046,529 (GRCm38)	missense	probably damaging	0.98
IGL03130:Tgm4	APN	9	123,056,515 (GRCm38)	missense	probably damaging	1.00
IGL03188:Tgm4	APN	9	123,045,036 (GRCm38)	missense	probably null	0.06
R0329:Tgm4	UTSW	9	123,048,557 (GRCm38)	critical splice donor site	probably null
R0480:Tgm4	UTSW	9	123,062,419 (GRCm38)	missense	probably benign
R0644:Tgm4	UTSW	9	123,051,458 (GRCm38)	missense	probably damaging	1.00
R0990:Tgm4	UTSW	9	123,046,511 (GRCm38)	missense	probably benign	0.02
R1604:Tgm4	UTSW	9	123,045,064 (GRCm38)	missense	probably benign	0.39
R1644:Tgm4	UTSW	9	123,051,416 (GRCm38)	missense	probably damaging	1.00
R2056:Tgm4	UTSW	9	123,061,770 (GRCm38)	missense	probably damaging	1.00
R2058:Tgm4	UTSW	9	123,061,770 (GRCm38)	missense	probably damaging	1.00
R2059:Tgm4	UTSW	9	123,061,770 (GRCm38)	missense	probably damaging	1.00
R2076:Tgm4	UTSW	9	123,051,095 (GRCm38)	missense	probably benign	0.24
R2437:Tgm4	UTSW	9	123,048,549 (GRCm38)	nonsense	probably null
R4392:Tgm4	UTSW	9	123,066,752 (GRCm38)	missense	probably benign	0.10
R4407:Tgm4	UTSW	9	123,056,530 (GRCm38)	missense	probably damaging	1.00
R4752:Tgm4	UTSW	9	123,051,386 (GRCm38)	missense	probably damaging	1.00
R5288:Tgm4	UTSW	9	123,056,494 (GRCm38)	missense	probably damaging	1.00
R5365:Tgm4	UTSW	9	123,066,801 (GRCm38)	missense	probably damaging	1.00
R5386:Tgm4	UTSW	9	123,056,494 (GRCm38)	missense	probably damaging	1.00
R5790:Tgm4	UTSW	9	123,061,743 (GRCm38)	missense	probably damaging	0.98
R5890:Tgm4	UTSW	9	123,061,638 (GRCm38)	missense	probably damaging	1.00
R6102:Tgm4	UTSW	9	123,056,535 (GRCm38)	missense	probably benign
R6358:Tgm4	UTSW	9	123,056,518 (GRCm38)	missense	probably damaging	1.00
R6956:Tgm4	UTSW	9	123,064,703 (GRCm38)	missense	possibly damaging	0.93
R6966:Tgm4	UTSW	9	123,051,142 (GRCm38)	missense	possibly damaging	0.68
R7091:Tgm4	UTSW	9	123,040,460 (GRCm38)	missense	probably damaging	1.00
R7258:Tgm4	UTSW	9	123,062,491 (GRCm38)	missense	probably benign	0.02
R7313:Tgm4	UTSW	9	123,062,491 (GRCm38)	missense	probably benign	0.02
R7369:Tgm4	UTSW	9	123,056,684 (GRCm38)	critical splice donor site	probably null
R7802:Tgm4	UTSW	9	123,051,336 (GRCm38)	intron	probably benign
R8219:Tgm4	UTSW	9	123,045,052 (GRCm38)	missense	probably benign
R8787:Tgm4	UTSW	9	123,061,845 (GRCm38)	missense	probably damaging	1.00
R8936:Tgm4	UTSW	9	123,040,476 (GRCm38)	missense	possibly damaging	0.92
R9045:Tgm4	UTSW	9	123,048,551 (GRCm38)	missense	possibly damaging	0.94
R9328:Tgm4	UTSW	9	123,056,632 (GRCm38)	missense	possibly damaging	0.93
R9359:Tgm4	UTSW	9	123,052,772 (GRCm38)	missense	probably damaging	1.00
R9403:Tgm4	UTSW	9	123,052,772 (GRCm38)	missense	probably damaging	1.00
R9471:Tgm4	UTSW	9	123,040,379 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGCATGTACAATGTATTGCTGTGAC -3'
(R):5'- AGCTTAGTAGTCCTTTCAGGCC -3'

Sequencing Primer
(F):5'- GTACAATGTATTGCTGTGACCCTTTC -3'
(R):5'- AGGCCACTTTCACACTTGTG -3'

Posted On

2022-11-14