Mutagenetix > Incidental Mutation

Incidental Mutation 'R9750:Zfp960'

732487

Institutional Source

Beutler Lab

Gene Symbol

Zfp960

Ensembl Gene

ENSMUSG00000096696

Gene Name

zinc finger protein 960

Synonyms

BC018101

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.229) question?

Stock #

R9750 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

17284375-17309890 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 17307898 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 204 (H204L)

Ref Sequence

ENSEMBL: ENSMUSP00000123102 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000127027]

AlphaFold

L7N267

Predicted Effect

probably damaging
Transcript: ENSMUST00000127027
AA Change: H204L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000123102
Gene: ENSMUSG00000096696
AA Change: H204L

Domain	Start	End	E-Value	Type
KRAB	13	82	4.42e-16	SMART
ZnF_C2H2	158	180	3.95e-4	SMART
ZnF_C2H2	186	208	4.4e-2	SMART
ZnF_C2H2	238	260	2.95e-3	SMART
ZnF_C2H2	266	288	6.32e-3	SMART
ZnF_C2H2	294	316	8.94e-3	SMART
ZnF_C2H2	322	344	2.53e-2	SMART
ZnF_C2H2	350	372	3.21e-4	SMART
ZnF_C2H2	378	400	3.89e-3	SMART
ZnF_C2H2	406	428	2.2e-2	SMART
ZnF_C2H2	434	456	7.9e-4	SMART
ZnF_C2H2	462	484	7.9e-4	SMART
ZnF_C2H2	490	512	7.78e-3	SMART
ZnF_C2H2	518	540	3.95e-4	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.2%
20x: 97.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts2	G	T	11: 50,494,333 (GRCm39)	V136L	probably benign	Het
Adamtsl3	G	T	7: 82,244,589 (GRCm39)	L1348F	probably benign	Het
Amotl1	T	A	9: 14,504,102 (GRCm39)	T369S	probably benign	Het
Anapc2	T	G	2: 25,174,982 (GRCm39)	V700G	possibly damaging	Het
Aox3	C	T	1: 58,215,648 (GRCm39)	Q1027*	probably null	Het
Ap5m1	A	G	14: 49,317,756 (GRCm39)	T338A	probably benign	Het
Carf	T	A	1: 60,171,158 (GRCm39)	Y240*	probably null	Het
Ccl21a	G	A	4: 42,773,875 (GRCm39)	A15V	probably benign	Het
Dcst1	A	T	3: 89,261,462 (GRCm39)	I429N	probably damaging	Het
Esco1	A	G	18: 10,594,510 (GRCm39)	S259P	probably benign	Het
Esp15	A	G	17: 39,955,603 (GRCm39)	M57V	probably benign	Het
Fat3	A	G	9: 15,915,157 (GRCm39)	I1597T	probably benign	Het
Fbxo38	T	C	18: 62,674,061 (GRCm39)	S7G	probably benign	Het
Gm11562	G	A	11: 99,510,856 (GRCm39)	P115S	unknown	Het
Hectd4	A	T	5: 121,448,744 (GRCm39)	Y364F	probably benign	Het
Kansl1l	G	C	1: 66,817,150 (GRCm39)	I403M	probably benign	Het
Kmt2a	T	C	9: 44,747,499 (GRCm39)	H1505R	unknown	Het
Krt79	T	G	15: 101,839,196 (GRCm39)	E424D	probably benign	Het
Lpgat1	T	A	1: 191,510,587 (GRCm39)	F391I	probably benign	Het
Ltbr	G	A	6: 125,284,348 (GRCm39)	R365W	probably damaging	Het
Mon1a	T	A	9: 107,778,778 (GRCm39)	V334E	probably damaging	Het
Ngef	G	A	1: 87,431,010 (GRCm39)	P269L	probably damaging	Het
Nup210l	A	T	3: 90,117,659 (GRCm39)		probably null	Het
Obscn	G	A	11: 59,024,682 (GRCm39)	R453W	probably benign	Het
Or1d2	A	G	11: 74,255,858 (GRCm39)	D121G	possibly damaging	Het
Pdzd8	A	G	19: 59,289,684 (GRCm39)	V572A	probably benign	Het
Pgr	T	C	9: 8,901,918 (GRCm39)	S484P	possibly damaging	Het
Pygl	G	T	12: 70,245,303 (GRCm39)	S473R	possibly damaging	Het
Samd1	T	C	8: 84,725,989 (GRCm39)	V410A	probably damaging	Het
Serpina3j	T	G	12: 104,280,942 (GRCm39)	H38Q	probably benign	Het
Shprh	A	G	10: 11,040,204 (GRCm39)	Y559C	probably damaging	Het
Skint5	T	A	4: 113,727,866 (GRCm39)	D426V	unknown	Het
Slc25a21	T	C	12: 56,785,382 (GRCm39)	K158R	probably benign	Het
Smc1b	A	T	15: 85,016,106 (GRCm39)	N11K	probably damaging	Het
St18	T	C	1: 6,873,216 (GRCm39)	V317A	probably benign	Het
Styk1	CTCTTCATGATTTTCTT	CTCTT	6: 131,278,612 (GRCm39)		probably benign	Het
Tcap	A	G	11: 98,275,228 (GRCm39)	T121A	probably benign	Het
Tgs1	C	A	4: 3,585,869 (GRCm39)	Q249K	probably damaging	Het
Tiam1	A	T	16: 89,695,394 (GRCm39)	L21Q	probably damaging	Het
Tmem131l	G	A	3: 83,831,358 (GRCm39)	A858V	probably damaging	Het
Trpm3	G	A	19: 22,903,495 (GRCm39)	R927H	probably benign	Het
Wdfy3	T	A	5: 102,077,960 (GRCm39)	H870L	probably benign	Het
Zfp518a	T	A	19: 40,903,889 (GRCm39)	C1273S	possibly damaging	Het

Other mutations in Zfp960

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1026:Zfp960	UTSW	17	17,308,518 (GRCm39)	missense	probably damaging	1.00
R1511:Zfp960	UTSW	17	17,308,518 (GRCm39)	missense	probably damaging	1.00
R2141:Zfp960	UTSW	17	17,308,146 (GRCm39)	missense	probably benign	0.09
R3729:Zfp960	UTSW	17	17,308,633 (GRCm39)	missense	probably damaging	1.00
R3730:Zfp960	UTSW	17	17,308,633 (GRCm39)	missense	probably damaging	1.00
R3731:Zfp960	UTSW	17	17,308,633 (GRCm39)	missense	probably damaging	1.00
R4799:Zfp960	UTSW	17	17,308,698 (GRCm39)	missense	probably damaging	1.00
R5418:Zfp960	UTSW	17	17,307,805 (GRCm39)	missense	probably damaging	1.00
R5513:Zfp960	UTSW	17	17,307,996 (GRCm39)	missense	possibly damaging	0.64
R6309:Zfp960	UTSW	17	17,308,639 (GRCm39)	missense	probably benign	0.08
R6836:Zfp960	UTSW	17	17,308,434 (GRCm39)	missense	probably damaging	1.00
R7166:Zfp960	UTSW	17	17,308,761 (GRCm39)	missense	probably damaging	1.00
R7528:Zfp960	UTSW	17	17,307,825 (GRCm39)	missense	possibly damaging	0.84
R7576:Zfp960	UTSW	17	17,308,227 (GRCm39)	missense	probably benign	0.05
R8063:Zfp960	UTSW	17	17,308,623 (GRCm39)	missense	probably benign	0.03
R8263:Zfp960	UTSW	17	17,308,202 (GRCm39)	nonsense	probably null
R8354:Zfp960	UTSW	17	17,308,461 (GRCm39)	missense	probably benign	0.00
R8454:Zfp960	UTSW	17	17,308,461 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACATAAGGACACTGGATCTTTCATG -3'
(R):5'- GGCACATTGGTTACATTGATAGAG -3'

Sequencing Primer
(F):5'- AGTCTATGATGGTAACCAATGTGG -3'
(R):5'- TGGTTACATTGATAGAGTTTTTCTCC -3'

Posted On

2022-11-14