Mutagenetix > Incidental Mutation

Incidental Mutation 'R9751:Tas2r115'

732513

Institutional Source

Beutler Lab

Gene Symbol

Tas2r115

Ensembl Gene

ENSMUSG00000071149

Gene Name

taste receptor, type 2, member 115

Synonyms

mGR15, T2R15, Tas2r15, mt2r49

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.049) question?

Stock #

R9751 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

132737003-132738035 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132737955 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 11 (I11T)

Ref Sequence

ENSEMBL: ENSMUSP00000093043 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095394]

AlphaFold

Q7M719

Predicted Effect

possibly damaging
Transcript: ENSMUST00000095394
AA Change: I11T

PolyPhen 2 Score 0.850 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000093043
Gene: ENSMUSG00000071149
AA Change: I11T

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	299	2.2e-84	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.5%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438A13Rik	A	G	3: 37,011,740	I54M		Het
Abca4	A	G	3: 122,087,477	N514D	probably benign	Het
Adgre1	G	A	17: 57,450,101	R786H	probably null	Het
Ankrd7	G	A	6: 18,868,025	V97I	probably damaging	Het
Bmper	C	T	9: 23,406,713	P543S	possibly damaging	Het
Brwd1	A	T	16: 95,993,815	M2233K	possibly damaging	Het
C1s2	G	T	6: 124,625,594	P553T	probably damaging	Het
Cachd1	G	A	4: 100,966,241	V497I	possibly damaging	Het
Cacng4	A	G	11: 107,735,193	S191P	probably damaging	Het
Cd109	C	A	9: 78,698,160	T1015K	probably damaging	Het
Clstn2	A	G	9: 97,457,650	L756P	probably damaging	Het
Crybg3	A	T	16: 59,557,524	D1122E	possibly damaging	Het
Csf2	A	T	11: 54,249,594	L6*	probably null	Het
Csnk1g2	T	A	10: 80,637,911	Y71N	possibly damaging	Het
Dlg2	C	A	7: 90,915,523	H116N	probably benign	Het
Dnah14	C	T	1: 181,792,045	S3978L	probably damaging	Het
Dpp8	C	T	9: 65,053,171	T328I	probably null	Het
Dysf	T	C	6: 84,186,468	V1625A	probably damaging	Het
Egf	C	A	3: 129,754,889	V26F	probably damaging	Het
Fam126a	C	T	5: 23,991,750	E47K	probably benign	Het
Fam20a	T	A	11: 109,675,166	Y414F	probably damaging	Het
Fsip2	T	C	2: 82,987,897	I4658T	probably benign	Het
Gm11639	G	A	11: 104,893,085	G2754E	probably benign	Het
Gm21319	T	C	12: 87,773,756	N11S	possibly damaging	Het
Igflr1	A	T	7: 30,567,228	Q167L	possibly damaging	Het
Krt79	T	G	15: 101,930,761	E424D	probably benign	Het
Map3k6	A	G	4: 133,251,857		probably null	Het
Mcpt4	A	G	14: 56,060,054	I215T	probably damaging	Het
Med13	T	C	11: 86,299,158	Y975C	probably damaging	Het
Meioc	T	A	11: 102,675,593	Y678*	probably null	Het
Myof	T	C	19: 37,936,370	T1190A	probably benign	Het
Nap1l4	A	C	7: 143,534,395		probably benign	Het
Ncapg	T	C	5: 45,693,853	V796A	probably damaging	Het
Olfr1167	T	A	2: 88,149,270	I250L	probably benign	Het
Olfr1168	T	A	2: 88,184,916	V13E	possibly damaging	Het
Olfr1224-ps1	C	T	2: 89,156,438	V246M	possibly damaging	Het
Olfr1257	C	T	2: 89,881,612	T262I	probably benign	Het
Olfr493	T	C	7: 108,346,438	Y181C	probably benign	Het
Paxbp1	A	G	16: 91,027,300	S515P	probably benign	Het
Plce1	C	T	19: 38,728,970	S1401F	probably damaging	Het
Rptor	A	G	11: 119,887,138	K1043E	probably benign	Het
Rtn4rl2	T	G	2: 84,880,695	N75T	probably damaging	Het
Slc35f4	A	G	14: 49,298,834	I448T	possibly damaging	Het
Styk1	CTCTTCATGATTTTCTT	CTCTT	6: 131,301,649		probably benign	Het
Tbc1d5	A	G	17: 50,874,652	V351A	possibly damaging	Het
Tmem55b	A	G	14: 50,927,979	V257A	probably benign	Het
Trim43b	A	T	9: 89,089,517	D195E	probably benign	Het
Trim65	C	A	11: 116,130,738	A90S	probably benign	Het
Trip11	C	T	12: 101,884,506	V1100I	possibly damaging	Het
Tyrp1	A	G	4: 80,840,775	E295G	probably null	Het
Ube2g2	G	A	10: 77,644,473	V138I	probably benign	Het
Wdr60	T	A	12: 116,241,783		probably null	Het

Other mutations in Tas2r115

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00565:Tas2r115	APN	6	132737778	missense	probably benign	0.11
IGL01285:Tas2r115	APN	6	132737678	missense	probably damaging	1.00
IGL01516:Tas2r115	APN	6	132737613	missense	probably damaging	0.98
IGL01637:Tas2r115	APN	6	132737629	missense	probably damaging	0.99
IGL02041:Tas2r115	APN	6	132737467	missense	probably benign	0.13
IGL02178:Tas2r115	APN	6	132737308	missense	probably benign	0.11
R0467:Tas2r115	UTSW	6	132737719	missense	probably benign	0.02
R0553:Tas2r115	UTSW	6	132737959	missense	probably benign	0.18
R1425:Tas2r115	UTSW	6	132737479	missense	probably benign	0.02
R1770:Tas2r115	UTSW	6	132737971	missense	probably damaging	1.00
R2120:Tas2r115	UTSW	6	132737507	missense	possibly damaging	0.51
R2136:Tas2r115	UTSW	6	132737346	missense	probably damaging	0.99
R2141:Tas2r115	UTSW	6	132737358	missense	probably benign	0.43
R2142:Tas2r115	UTSW	6	132737358	missense	probably benign	0.43
R4479:Tas2r115	UTSW	6	132737532	missense	probably damaging	0.98
R4687:Tas2r115	UTSW	6	132737284	missense	possibly damaging	0.80
R4948:Tas2r115	UTSW	6	132737161	missense	probably damaging	1.00
R5097:Tas2r115	UTSW	6	132737253	missense	probably damaging	1.00
R5856:Tas2r115	UTSW	6	132737538	missense	possibly damaging	0.86
R6927:Tas2r115	UTSW	6	132737932	missense	probably damaging	1.00
R7473:Tas2r115	UTSW	6	132737251	missense	probably damaging	1.00
R7688:Tas2r115	UTSW	6	132737680	missense	probably damaging	1.00
R8415:Tas2r115	UTSW	6	132737835	missense	probably damaging	1.00
R8495:Tas2r115	UTSW	6	132737924	missense	probably damaging	1.00
R9032:Tas2r115	UTSW	6	132737364	missense	probably benign	0.37
R9085:Tas2r115	UTSW	6	132737364	missense	probably benign	0.37
R9318:Tas2r115	UTSW	6	132737509	missense	probably benign	0.01
R9665:Tas2r115	UTSW	6	132737427	missense	probably benign	0.31
Z1088:Tas2r115	UTSW	6	132737081	nonsense	probably null
Z1176:Tas2r115	UTSW	6	132737856	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGGCAGTCTGAATCAGTCTTAATG -3'
(R):5'- CCTGGAAGAATTTCAGCCAAGC -3'

Sequencing Primer
(F):5'- TCTAGTCATCAGTAATGGGGATTG -3'
(R):5'- GCTGGAGGTAAAAATCCAAAATTCTG -3'

Posted On

2022-11-14