Mutagenetix > Incidental Mutation

Incidental Mutation 'R9752:Or7e176'

732581

Institutional Source

Beutler Lab

Gene Symbol

Or7e176

Ensembl Gene

ENSMUSG00000066897

Gene Name

olfactory receptor family 7 subfamily E member 176

Synonyms

Olfr872, GA_x6K02T2PVTD-13999915-14000844, MOR145-3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R9752 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20148458-20172209 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 20171204 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 23 (V23I)

Ref Sequence

ENSEMBL: ENSMUSP00000083665 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086474]

AlphaFold

E9PVW2

Predicted Effect

probably benign
Transcript: ENSMUST00000086474
AA Change: V23I

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000083665
Gene: ENSMUSG00000066897
AA Change: V23I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	45	321	3.8e-45	PFAM
Pfam:7TM_GPCR_Srsx	49	310	1.4e-9	PFAM
Pfam:7tm_1	55	304	4.2e-21	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 99.3%
20x: 98.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano2	C	T	6: 125,840,499 (GRCm39)	T390I	probably damaging	Het
Cc2d2b	T	C	19: 40,781,271 (GRCm39)	V772A	probably damaging	Het
Cd109	A	G	9: 78,614,834 (GRCm39)	I1294V	probably benign	Het
Cic	A	G	7: 24,971,403 (GRCm39)	E378G	probably damaging	Het
Clhc1	C	T	11: 29,507,778 (GRCm39)	P175L	probably benign	Het
Clip1	T	C	5: 123,760,009 (GRCm39)	E783G	probably damaging	Het
Cnot1	A	G	8: 96,488,019 (GRCm39)	Y546H	probably damaging	Het
Cog7	C	A	7: 121,580,639 (GRCm39)		probably null	Het
Cr2	T	C	1: 194,823,575 (GRCm39)	T1008A	probably benign	Het
Diaph1	T	C	18: 38,036,124 (GRCm39)	T196A	unknown	Het
Entr1	T	C	2: 26,276,990 (GRCm39)	N247D	probably benign	Het
Flg2	A	T	3: 93,108,467 (GRCm39)	D165V	probably damaging	Het
Fstl1	T	G	16: 37,649,534 (GRCm39)	N249K	probably damaging	Het
Gatad2a	A	G	8: 70,364,839 (GRCm39)	V515A	probably benign	Het
Gbp2b	A	T	3: 142,313,917 (GRCm39)	D399V	probably benign	Het
Gna14	G	A	19: 16,586,781 (GRCm39)	V319I	probably benign	Het
Grip1	A	G	10: 119,871,256 (GRCm39)	D661G	possibly damaging	Het
Gtf3c4	C	A	2: 28,724,126 (GRCm39)	L535F	probably damaging	Het
Haus8	A	C	8: 71,715,731 (GRCm39)	S36A	probably damaging	Het
Hectd4	A	T	5: 121,448,744 (GRCm39)	Y364F	probably benign	Het
Hectd4	C	T	5: 121,472,415 (GRCm39)	A2717V	possibly damaging	Het
Hsf5	C	T	11: 87,513,709 (GRCm39)	T258M	probably benign	Het
Ifi209	A	T	1: 173,472,235 (GRCm39)	I359F	probably damaging	Het
Ighmbp2	A	G	19: 3,324,360 (GRCm39)	V302A	probably benign	Het
Iqgap3	C	A	3: 88,016,176 (GRCm39)	F986L	probably damaging	Het
Itgav	T	A	2: 83,600,451 (GRCm39)		probably null	Het
Kcp	A	T	6: 29,497,754 (GRCm39)	D502E	probably damaging	Het
Kif15	T	C	9: 122,824,890 (GRCm39)		probably null	Het
Lipo2	C	G	19: 33,723,221 (GRCm39)	K189N	possibly damaging	Het
Lrch4	T	A	5: 137,636,218 (GRCm39)	S390T	probably benign	Het
Mast2	G	T	4: 116,179,508 (GRCm39)	H389N	probably benign	Het
Med13	A	G	11: 86,174,147 (GRCm39)	Y1784H	possibly damaging	Het
Micu3	A	G	8: 40,833,751 (GRCm39)	K462E	possibly damaging	Het
Mrps10	T	C	17: 47,683,572 (GRCm39)		probably null	Het
Msh6	T	C	17: 88,293,963 (GRCm39)	L906P	probably damaging	Het
Nphp4	A	G	4: 152,621,737 (GRCm39)	E531G	probably benign	Het
Nrp2	A	T	1: 62,851,726 (GRCm39)	Y825F	probably benign	Het
Ntn1	C	T	11: 68,276,712 (GRCm39)	V79M	possibly damaging	Het
Nxnl2	A	G	13: 51,325,507 (GRCm39)	D50G	probably damaging	Het
Or1e16	T	A	11: 73,286,479 (GRCm39)	Y123F	possibly damaging	Het
Or2t26	T	C	11: 49,039,681 (GRCm39)	M199T	probably benign	Het
Or52h9	A	T	7: 104,202,530 (GRCm39)	I135F	possibly damaging	Het
Or52r1c	T	A	7: 102,735,669 (GRCm39)	C310S	probably benign	Het
Or6c219	T	C	10: 129,780,990 (GRCm39)	R314G	probably benign	Het
Ppp1r42	A	G	1: 10,073,674 (GRCm39)		probably benign	Het
Ppp4r3a	A	T	12: 101,008,763 (GRCm39)	N692K	probably benign	Het
Prr11	T	C	11: 86,994,416 (GRCm39)	T77A	possibly damaging	Het
Resf1	T	A	6: 149,228,068 (GRCm39)	N371K	probably benign	Het
Rrn3	A	G	16: 13,631,095 (GRCm39)	I644V	probably benign	Het
Scd4	T	C	19: 44,322,475 (GRCm39)	S23P	probably benign	Het
Slc26a2	T	A	18: 61,335,010 (GRCm39)	S148C	probably benign	Het
Slc7a8	T	C	14: 54,995,931 (GRCm39)	E157G	probably benign	Het
Spi1	A	G	2: 90,943,666 (GRCm39)	H70R	probably benign	Het
Styk1	CTCTTCATGATTTTCTT	CTCTT	6: 131,278,612 (GRCm39)		probably benign	Het
Tlr4	A	G	4: 66,757,912 (GRCm39)	N235S	probably benign	Het
Trpc6	C	A	9: 8,643,641 (GRCm39)	S475R	probably benign	Het
Usp34	C	T	11: 23,409,182 (GRCm39)	P2674L	probably benign	Het
Usp48	G	A	4: 137,341,137 (GRCm39)	G379D	probably damaging	Het
Vkorc1l1	T	A	5: 130,011,078 (GRCm39)	C97*	probably null	Het
Wfikkn2	T	C	11: 94,129,211 (GRCm39)	E310G	probably benign	Het
Zfp266	T	A	9: 20,411,496 (GRCm39)	N227I	probably benign	Het
Zfp335	C	T	2: 164,749,347 (GRCm39)		probably null	Het
Zfp677	T	A	17: 21,618,511 (GRCm39)	C523S	probably damaging	Het
Zrsr2	GCTCCTCCTCCTCCTCCT	GCTCCTCCTCCTCCT	X: 162,719,750 (GRCm39)		probably benign	Het

Other mutations in Or7e176

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01904:Or7e176	APN	9	20,171,586 (GRCm39)	missense	probably damaging	0.99
IGL02048:Or7e176	APN	9	20,171,784 (GRCm39)	missense	possibly damaging	0.79
IGL02232:Or7e176	APN	9	20,171,511 (GRCm39)	missense	probably damaging	1.00
IGL02314:Or7e176	APN	9	20,171,774 (GRCm39)	missense	probably benign	0.03
IGL03290:Or7e176	APN	9	20,171,556 (GRCm39)	missense	probably damaging	1.00
R0410:Or7e176	UTSW	9	20,171,797 (GRCm39)	missense	probably benign	0.03
R1482:Or7e176	UTSW	9	20,172,020 (GRCm39)	missense	possibly damaging	0.89
R1521:Or7e176	UTSW	9	20,171,728 (GRCm39)	missense	possibly damaging	0.91
R4930:Or7e176	UTSW	9	20,171,313 (GRCm39)	missense	probably damaging	1.00
R5457:Or7e176	UTSW	9	20,171,574 (GRCm39)	missense	probably damaging	1.00
R5870:Or7e176	UTSW	9	20,171,874 (GRCm39)	missense	probably benign
R6141:Or7e176	UTSW	9	20,171,754 (GRCm39)	missense	probably benign	0.00
R7283:Or7e176	UTSW	9	20,171,555 (GRCm39)	missense	probably damaging	0.98
R8691:Or7e176	UTSW	9	20,171,747 (GRCm39)	missense	probably benign	0.36
R8882:Or7e176	UTSW	9	20,171,256 (GRCm39)	missense	probably benign	0.06
R9556:Or7e176	UTSW	9	20,171,651 (GRCm39)	missense	probably benign	0.00
R9618:Or7e176	UTSW	9	20,171,639 (GRCm39)	missense	possibly damaging	0.80
X0025:Or7e176	UTSW	9	20,171,782 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGAGGCTGGAAGCTATGGAC -3'
(R):5'- GTGGAGATCAAACAAATGTCAGACC -3'

Sequencing Primer
(F):5'- CTGGAAGCTATGGACCTCAAGTG -3'
(R):5'- TGTCAGACCAGGAAAGGTTG -3'

Posted On

2022-11-14