Mutagenetix > Incidental Mutation

Incidental Mutation 'R9760:Micall1'

732974

Institutional Source

Beutler Lab

Gene Symbol

Micall1

Ensembl Gene

ENSMUSG00000033039

Gene Name

microtubule associated monooxygenase, calponin and LIM domain containing -like 1

Synonyms

Mus EST 820961, D15Mit260, D15N2e

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R9760 (G1)

Quality Score

222.009

Status

Not validated

Chromosome

Chromosomal Location

78993098-79021100 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 79005032 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 168 (C168Y)

Ref Sequence

ENSEMBL: ENSMUSP00000042053 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040320] [ENSMUST00000229031]

AlphaFold

Q8BGT6

Predicted Effect

unknown
Transcript: ENSMUST00000040320
AA Change: C168Y

SMART Domains

Protein: ENSMUSP00000042053
Gene: ENSMUSG00000033039
AA Change: C168Y

Domain	Start	End	E-Value	Type
CH	4	103	5.64e-19	SMART
low complexity region	113	135	N/A	INTRINSIC
LIM	164	219	1.15e-5	SMART
low complexity region	241	250	N/A	INTRINSIC
low complexity region	375	394	N/A	INTRINSIC
low complexity region	414	467	N/A	INTRINSIC
low complexity region	477	497	N/A	INTRINSIC
low complexity region	515	530	N/A	INTRINSIC
low complexity region	571	586	N/A	INTRINSIC
DUF3585	685	825	5.07e-68	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000229004

Predicted Effect

probably damaging
Transcript: ENSMUST00000229031
AA Change: C168Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 99.1%
20x: 97.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abat	T	C	16: 8,399,794 (GRCm39)		probably null	Het
Adamts4	G	A	1: 171,086,334 (GRCm39)	D709N	probably benign	Het
Arhgef12	G	T	9: 42,903,318 (GRCm39)	D745E	probably damaging	Het
Cep250	A	G	2: 155,818,473 (GRCm39)	K883E	probably benign	Het
Cfdp1	T	C	8: 112,495,415 (GRCm39)	I268V	probably benign	Het
Cgnl1	G	T	9: 71,552,853 (GRCm39)	S1037*	probably null	Het
Clca3b	T	C	3: 144,552,610 (GRCm39)	N142S	probably benign	Het
Col6a6	A	T	9: 105,659,253 (GRCm39)	I564N	probably damaging	Het
Coq4	G	T	2: 29,678,482 (GRCm39)	R36L	probably benign	Het
Dact3	G	A	7: 16,620,131 (GRCm39)	S542N	unknown	Het
Dcaf1	A	G	9: 106,751,466 (GRCm39)	D1480G	unknown	Het
Dcdc2a	C	T	13: 25,389,443 (GRCm39)	T457I	probably damaging	Het
Ddi2	A	G	4: 141,411,196 (GRCm39)	V572A	probably damaging	Het
Ddx54	C	T	5: 120,761,672 (GRCm39)	R483C	probably benign	Het
Dennd5b	G	A	6: 148,969,997 (GRCm39)	S152F	probably benign	Het
Efcab14	C	T	4: 115,616,072 (GRCm39)	H252Y	probably benign	Het
Efhb	A	T	17: 53,770,298 (GRCm39)	F4I	probably damaging	Het
Esrrg	A	G	1: 187,775,569 (GRCm39)	D32G	probably benign	Het
Far2	C	A	6: 148,060,448 (GRCm39)	A267E	probably damaging	Het
Fhip2b	A	C	14: 70,827,621 (GRCm39)	V158G	possibly damaging	Het
Flnb	T	C	14: 7,929,846 (GRCm38)	I1992T	probably damaging	Het
Fmnl2	A	G	2: 52,944,527 (GRCm39)	S169G		Het
Galnt10	G	A	11: 57,656,514 (GRCm39)	V233I	probably benign	Het
Gbf1	C	A	19: 46,244,137 (GRCm39)	N210K	probably benign	Het
Gbp3	T	G	3: 142,276,283 (GRCm39)	S460A	probably benign	Het
Gm12185	T	A	11: 48,806,168 (GRCm39)	H341L	probably benign	Het
Gm5592	A	T	7: 40,939,234 (GRCm39)	I839F	possibly damaging	Het
Gnptab	A	G	10: 88,267,310 (GRCm39)	D467G	probably damaging	Het
Grin3a	A	T	4: 49,714,213 (GRCm39)	M844K	probably damaging	Het
Gtf2a1l	A	G	17: 89,019,020 (GRCm39)	D368G	probably benign	Het
Herc2	G	C	7: 55,813,659 (GRCm39)		probably null	Het
Hycc1	T	C	5: 24,184,572 (GRCm39)	Q302R	possibly damaging	Het
Ins2	T	A	7: 142,233,185 (GRCm39)	H29L	probably damaging	Het
Ipo13	T	A	4: 117,762,778 (GRCm39)	Y275F	probably benign	Het
Kcnj15	T	A	16: 95,096,483 (GRCm39)	M35K	probably benign	Het
Kif21b	G	A	1: 136,076,421 (GRCm39)	V321M	probably damaging	Het
Lcmt1	T	A	7: 123,029,375 (GRCm39)	Y332*	probably null	Het
Malt1	A	G	18: 65,581,283 (GRCm39)	Q237R	probably benign	Het
Muc5ac	A	G	7: 141,360,985 (GRCm39)	K1432R	probably benign	Het
Nacad	A	G	11: 6,551,662 (GRCm39)	S510P	probably benign	Het
Nub1	T	C	5: 24,897,965 (GRCm39)	L117P	possibly damaging	Het
Or10al3	T	C	17: 38,012,434 (GRCm39)	L291P	probably damaging	Het
Or5af1	A	G	11: 58,722,852 (GRCm39)	R291G	probably damaging	Het
Or8g24	A	T	9: 38,989,271 (GRCm39)	F257I	possibly damaging	Het
Pkdrej	T	C	15: 85,705,268 (GRCm39)	K223E	probably benign	Het
Prkdc	T	A	16: 15,657,044 (GRCm39)	Y4046*	probably null	Het
Prpf8	A	G	11: 75,394,257 (GRCm39)	N1767D	probably benign	Het
Prr27	C	A	5: 87,990,994 (GRCm39)	P202Q	probably benign	Het
R3hdm2	G	T	10: 127,280,182 (GRCm39)	M18I	unknown	Het
Rab35	C	A	5: 115,778,224 (GRCm39)	D63E	probably damaging	Het
Ribc2	T	A	15: 85,027,568 (GRCm39)	Y350N	probably benign	Het
Sec63	A	G	10: 42,704,944 (GRCm39)	I733V	probably benign	Het
Slc23a3	ATT	ATTT	1: 75,109,925 (GRCm39)		probably null	Het
Slc38a4	T	C	15: 96,896,332 (GRCm39)	K512E	probably damaging	Het
Stk35	G	A	2: 129,642,605 (GRCm39)	V49I	probably benign	Het
Tango6	A	G	8: 107,576,911 (GRCm39)	E1055G	probably damaging	Het
Tas2r140	A	G	6: 40,469,036 (GRCm39)	I289V	probably benign	Het
Thoc2l	T	C	5: 104,667,101 (GRCm39)	V541A	probably benign	Het
Tll2	C	T	19: 41,119,084 (GRCm39)	V215M	probably damaging	Het
Tmem30a	A	T	9: 79,687,874 (GRCm39)	N98K	probably benign	Het
Trav9n-4	C	A	14: 53,532,290 (GRCm39)	A48E	probably benign	Het
Vinac1	A	T	2: 128,880,499 (GRCm39)	S476T		Het
Vmn1r192	A	G	13: 22,372,010 (GRCm39)	F70S	probably damaging	Het
Wdr89	A	G	12: 75,680,026 (GRCm39)	V76A	probably damaging	Het
Zfp229	A	T	17: 21,965,275 (GRCm39)	T502S	probably damaging	Het

Other mutations in Micall1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Micall1	APN	15	78,999,221 (GRCm39)	splice site	probably benign
IGL00496:Micall1	APN	15	78,999,221 (GRCm39)	splice site	probably benign
IGL00508:Micall1	APN	15	79,014,768 (GRCm39)	missense	probably damaging	0.99
IGL01626:Micall1	APN	15	79,014,712 (GRCm39)	missense	possibly damaging	0.46
IGL01868:Micall1	APN	15	78,999,260 (GRCm39)	missense	probably benign	0.41
IGL03062:Micall1	APN	15	78,998,881 (GRCm39)	missense	probably damaging	1.00
R0086:Micall1	UTSW	15	79,009,689 (GRCm39)	utr 3 prime	probably benign
R0099:Micall1	UTSW	15	79,016,101 (GRCm39)	splice site	probably benign
R0282:Micall1	UTSW	15	79,016,101 (GRCm39)	splice site	probably benign
R0727:Micall1	UTSW	15	79,004,978 (GRCm39)	missense	probably benign	0.00
R1859:Micall1	UTSW	15	79,007,145 (GRCm39)	intron	probably benign
R2142:Micall1	UTSW	15	79,014,995 (GRCm39)	missense	probably damaging	0.98
R2228:Micall1	UTSW	15	79,014,036 (GRCm39)	missense	probably damaging	1.00
R3508:Micall1	UTSW	15	79,006,965 (GRCm39)	missense	probably damaging	1.00
R4858:Micall1	UTSW	15	79,007,146 (GRCm39)	intron	probably benign
R4888:Micall1	UTSW	15	79,016,048 (GRCm39)	nonsense	probably null
R5059:Micall1	UTSW	15	79,007,034 (GRCm39)	intron	probably benign
R5097:Micall1	UTSW	15	79,014,078 (GRCm39)	missense	probably benign	0.17
R5451:Micall1	UTSW	15	79,011,104 (GRCm39)	splice site	probably null
R5710:Micall1	UTSW	15	79,011,290 (GRCm39)	missense	probably damaging	1.00
R5727:Micall1	UTSW	15	79,014,678 (GRCm39)	missense	possibly damaging	0.64
R7135:Micall1	UTSW	15	78,993,624 (GRCm39)	missense	unknown
R7186:Micall1	UTSW	15	79,009,575 (GRCm39)	missense	unknown
R7297:Micall1	UTSW	15	79,005,097 (GRCm39)	missense	unknown
R7472:Micall1	UTSW	15	79,006,760 (GRCm39)	missense	unknown
R8494:Micall1	UTSW	15	79,005,080 (GRCm39)	missense	probably damaging	1.00
R8714:Micall1	UTSW	15	79,011,510 (GRCm39)	missense	probably benign	0.03
R8937:Micall1	UTSW	15	79,011,198 (GRCm39)	missense	probably damaging	1.00
R9440:Micall1	UTSW	15	79,011,159 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- TTCCTGGGTTAGCACAGTGG -3'
(R):5'- AGTTTGTTCCTCTAGAAAGGGAG -3'

Sequencing Primer
(F):5'- AGCACAGTGGGGCTTGATGTAC -3'
(R):5'- TGAGCTCTCAGCCAGAAGC -3'

Posted On

2022-11-14